Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 437,076 A→T 7.8% K24N (AAA→AAT)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273437,0760AT 7.8% 642.3 / 32.3 201K24N (AAA→AAT) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (93/92);  new base T (11/5);  total (104/97)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

ATGCAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCC  >  CP009273/436929‑437224
                                                                                                                                                   |                                                                                                                                                    
aTGCAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAAt                                                                                                                                                    >  2:570824/1‑150 (MQ=255)
   cAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCt                                                                                                                                                 >  1:1729550/1‑150 (MQ=255)
    aGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAATATCCtt                                                                                                                                                >  1:504952/1‑150 (MQ=255)
    aGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCtt                                                                                                                                                <  2:2902539/150‑1 (MQ=255)
     gCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTa                                                                                                                                               >  1:2246518/1‑150 (MQ=255)
       cGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTAcc                                                                                                                                             <  1:12071/150‑1 (MQ=255)
       cGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTAcc                                                                                                                                             <  1:4099207/150‑1 (MQ=255)
        gCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCg                                                                                                                                            >  2:1345145/1‑150 (MQ=255)
          aGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCggg                                                                                                                                          <  1:1076637/150‑1 (MQ=255)
          aGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCggg                                                                                                                                          >  2:913922/1‑150 (MQ=255)
            gtgtGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGaa                                                                                                                                        >  2:4520945/1‑150 (MQ=255)
             tgtgGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAAc                                                                                                                                       <  2:3687220/150‑1 (MQ=255)
               tgGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACAt                                                                                                                                     <  1:2801051/150‑1 (MQ=255)
                gggCTGCTGTATTTTTCGGTTTACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACAtt                                                                                                                                    <  1:4287393/150‑1 (MQ=255)
                 ggCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTc                                                                                                                                   <  2:4179496/150‑1 (MQ=255)
                   ctgctgTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGt                                                                                                                                 >  2:393103/1‑150 (MQ=255)
                   ctgctgTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGt                                                                                                                                 >  2:1545297/1‑150 (MQ=255)
                   ctgctgTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGt                                                                                                                                 <  2:4195690/150‑1 (MQ=255)
                        gTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGt                                                                                                                            <  2:92906/150‑1 (MQ=255)
                         tATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGtt                                                                                                                           >  1:2416653/1‑150 (MQ=255)
                           tttttCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGtttt                                                                                                                         <  1:218857/150‑1 (MQ=255)
                              ttCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTaaa                                                                                                                      <  1:4344576/150‑1 (MQ=255)
                              ttCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTaaa                                                                                                                      <  2:648855/150‑1 (MQ=255)
                                 ccTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAATGCa                                                                                                                   >  2:2794657/1‑150 (MQ=255)
                                    ataCAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTa                                                                                                                <  2:2595271/150‑1 (MQ=255)
                                        aaGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTatgat                                                                                                            <  1:243656/150‑1 (MQ=255)
                                         aGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTatgatg                                                                                                           >  1:3073919/1‑150 (MQ=255)
                                          gTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTAtgatga                                                                                                          <  1:990020/150‑1 (MQ=255)
                                           tCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATgag                                                                                                         >  1:308331/1‑150 (MQ=255)
                                            cGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATgaga                                                                                                        >  2:1575927/1‑150 (MQ=255)
                                            cGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATgaga                                                                                                        >  2:1576638/1‑150 (MQ=255)
                                              cTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGAcg                                                                                                      >  2:1906058/1‑150 (MQ=255)
                                               ttAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGAcgc                                                                                                     >  2:877637/1‑150 (MQ=255)
                                                tAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGAcgct                                                                                                    <  1:262650/150‑1 (MQ=255)
                                                 aaGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGAcgctc                                                                                                   >  2:4390058/1‑150 (MQ=255)
                                                 aaGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGAcgctc                                                                                                   >  2:900359/1‑150 (MQ=255)
                                                    gCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGAcgctcgct                                                                                                <  2:763165/150‑1 (MQ=255)
                                                     cTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAATATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTg                                                                                               >  2:3616031/1‑150 (MQ=255)
                                                       tGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAATATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTgtc                                                                                             >  2:2635522/1‑150 (MQ=255)
                                                        gCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCGTGCGCTTGATAAAAATCCTTACCGGGAACATGCGTAACGTTGTAAAGCACTATGATGTGACGCTCGCTgtcg                                                                                            >  2:3973772/1‑150 (MQ=255)
                                                          cAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTgtcgtc                                                                                          >  2:1028331/1‑150 (MQ=255)
                                                           aaCGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTcc                                                                                         <  1:2386730/150‑1 (MQ=255)
                                                                   aTTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTCTGATGAGACGCTCGCTGTCGTCCGCCACTgg                                                                                 >  2:3142518/1‑150 (MQ=255)
                                                                   aTTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTgg                                                                                 >  2:3143080/1‑150 (MQ=255)
                                                                     tGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGa                                                                               >  2:2074369/1‑150 (MQ=255)
                                                                      gccgccATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGAt                                                                              >  1:1332582/1‑150 (MQ=255)
                                                                       ccgccATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATa                                                                             <  1:156477/150‑1 (MQ=255)
                                                                        cgccATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATaa                                                                            >  2:3017253/1‑150 (MQ=255)
                                                                           cATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACAt                                                                         <  1:703202/150‑1 (MQ=255)
                                                                           cATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACAt                                                                         <  1:14329/150‑1 (MQ=255)
                                                                           cATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACAt                                                                         <  1:2321366/150‑1 (MQ=255)
                                                                             tGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCg                                                                       <  1:1870620/150‑1 (MQ=255)
                                                                                  tttATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGtt                                                                  >  1:3955574/1‑150 (MQ=255)
                                                                                   ttATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTc                                                                 <  2:3943993/150‑1 (MQ=255)
                                                                                    tATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTcg                                                                >  1:4522863/1‑150 (MQ=255)
                                                                                     aTCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTcgc                                                               >  2:3798891/1‑150 (MQ=255)
                                                                                           aaaTTGTTCCCGGAAATCACCATCAACAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCaaaa                                                         <  2:2441867/150‑1 (MQ=255)
                                                                                            aaTTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAg                                                        <  1:3078557/150‑1 (MQ=255)
                                                                                            aaTTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAg                                                        <  1:3077478/150‑1 (MQ=255)
                                                                                             aTTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAATATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGa                                                       >  2:839038/1‑150 (MQ=255)
                                                                                             aTTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAATATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGa                                                       >  2:3954727/1‑150 (MQ=255)
                                                                                               tGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATg                                                     <  1:1898812/150‑1 (MQ=255)
                                                                                                gTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGa                                                    >  2:3468211/1‑150 (MQ=255)
                                                                                                gTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGa                                                    <  1:4478643/150‑1 (MQ=255)
                                                                                                 ttCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGaa                                                   >  1:4064834/1‑150 (MQ=255)
                                                                                                   cccGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGaaaa                                                 >  2:3311520/1‑150 (MQ=255)
                                                                                                     cGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAAcc                                               <  2:1233546/150‑1 (MQ=255)
                                                                                                      ggAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGACCATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCa                                              >  2:681562/1‑150 (MQ=255)
                                                                                                      ggAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGACGTTCGCGCAAAAGATGAAAACCa                                              >  2:1240475/1‑150 (MQ=255)
                                                                                                       gAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAg                                             <  1:4124990/150‑1 (MQ=255)
                                                                                                        aaaTCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGc                                            >  2:982295/1‑150 (MQ=255)
                                                                                                        aaaTCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGc                                            <  1:2679585/150‑1 (MQ=255)
                                                                                                          aTCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGt                                          <  1:2155442/150‑1 (MQ=255)
                                                                                                          aTCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGt                                          >  1:785198/1‑150 (MQ=255)
                                                                                                            cACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCt                                        >  2:3106384/1‑150 (MQ=255)
                                                                                                             aCCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTg                                       <  2:1720164/150‑1 (MQ=255)
                                                                                                              ccATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTgg                                      <  2:2516696/150‑1 (MQ=255)
                                                                                                               cATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGc                                     >  2:3327534/1‑150 (MQ=255)
                                                                                                                aTCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAATATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCt                                    <  1:2462006/150‑1 (MQ=255)
                                                                                                                  cAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTAt                                  <  2:1686452/150‑1 (MQ=255)
                                                                                                                     aaGCCAATCTGTGCGCTTGCGCTTTATAAATATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTcg                               <  2:4459369/150‑1 (MQ=255)
                                                                                                                     aaGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTcg                               <  1:3898343/150‑1 (MQ=255)
                                                                                                                         cAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGAc                           <  2:3844704/150‑1 (MQ=255)
                                                                                                                          aaTCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACg                          <  1:913922/150‑1 (MQ=255)
                                                                                                                          aaTCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACg                          >  2:161489/1‑150 (MQ=255)
                                                                                                                            tCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGct                        <  2:4221007/150‑1 (MQ=255)
                                                                                                                            tCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGct                        >  1:3482945/1‑150 (MQ=255)
                                                                                                                             cTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGctc                       >  1:1037569/1‑150 (MQ=255)
                                                                                                                             cTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGctc                       >  1:2521698/1‑150 (MQ=255)
                                                                                                                               gtgCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTg                     <  1:564797/150‑1 (MQ=255)
                                                                                                                               gtgCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTg                     <  1:646421/150‑1 (MQ=255)
                                                                                                                                  cgcttgcgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGAcc                  >  2:408091/1‑150 (MQ=255)
                                                                                                                                  cgctagcgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTATAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACAGGGATAACATCGAAGATCGCGCAAAAGAAGAAAACCAGCGTCTGGCTATTCGCGACTCTCTGAcc                  >  1:3839969/1‑150 (MQ=255)
                                                                                                                                   gcttgcgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGAccc                 >  1:2832548/1‑150 (MQ=255)
                                                                                                                                        cgcttTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTAtt            >  2:3045920/1‑150 (MQ=255)
                                                                                                                                               taAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTa     >  1:2902201/1‑150 (MQ=255)
                                                                                                                                               taAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTa     <  1:1543666/150‑1 (MQ=255)
                                                                                                                                                aaaaaTCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTAt    <  1:2006508/150‑1 (MQ=255)
                                                                                                                                                aaaaaTCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACGGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTAt    >  2:3699172/1‑150 (MQ=255)
                                                                                                                                                  aaaTCCTGACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATcc  <  1:3729502/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATGCAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAAAACCAGCGTCTGGCTATTCGCGACGCTCTGACCCGTATTCCGGGTATCC  >  CP009273/436929‑437224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑