Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 440,793 G→T 5.3% R444S (CGC→AGC)  yajR ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273440,7930GT 5.3% 987.7 / 20.7 262R444S (CGC→AGC) yajRputative transporter
Reads supporting (aligned to +/- strand):  ref base G (84/164);  new base T (3/11);  total (87/175)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 269 total aligned reads displayed.

CGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGA  >  CP009273/440644‑440942
                                                                                                                                                     |                                                                                                                                                     
cGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCg                                                                                                                                                       >  1:920796/1‑150 (MQ=255)
  ttttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGAt                                                                                                                                                     <  1:1691538/150‑1 (MQ=255)
   tttGTTCTTGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGAtt                                                                                                                                                    <  2:3982384/150‑1 (MQ=255)
    ttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAACGCGATTc                                                                                                                                                   <  2:4295419/150‑1 (MQ=255)
    ttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTc                                                                                                                                                   <  2:4086107/150‑1 (MQ=255)
    ttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTc                                                                                                                                                   <  1:1874309/150‑1 (MQ=255)
    ttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTc                                                                                                                                                   <  2:1986861/150‑1 (MQ=255)
    ttGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTc                                                                                                                                                   <  2:1554570/150‑1 (MQ=255)
     tGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCg                                                                                                                                                  <  2:2335777/150‑1 (MQ=255)
          aTGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACt                                                                                                                                             <  1:247488/150‑1 (MQ=255)
            gTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACttt                                                                                                                                           <  1:3595234/150‑1 (MQ=255)
            gTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACttt                                                                                                                                           <  1:3981606/150‑1 (MQ=255)
            gTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACttt                                                                                                                                           <  1:135262/150‑1 (MQ=255)
            gTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACttt                                                                                                                                           <  2:1239658/150‑1 (MQ=255)
              cAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGc                                                                                                                                         <  1:1469943/150‑1 (MQ=255)
                gATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTg                                                                                                                                       <  2:892424/150‑1 (MQ=255)
                gATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTg                                                                                                                                       >  2:2178433/1‑150 (MQ=255)
                gATGCGGAATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTg                                                                                                                                       <  2:2970503/150‑1 (MQ=255)
                 aTGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGt                                                                                                                                      >  1:1278122/1‑150 (MQ=255)
                  tGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTc                                                                                                                                     <  1:1759739/150‑1 (MQ=255)
                   gCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCg                                                                                                                                    <  2:1049243/150‑1 (MQ=255)
                    cGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGa                                                                                                                                   >  1:1081470/1‑150 (MQ=255)
                      gCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGAtt                                                                                                                                 <  2:111016/150‑1 (MQ=255)
                        aTGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGAtttt                                                                                                                               <  2:4243658/150‑1 (MQ=255)
                        aTGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAtttt                                                                                                                               <  1:3116149/150‑1 (MQ=255)
                                      cGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAAt                                                                                                                 <  1:669038/150‑1 (MQ=255)
                                       ggCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATg                                                                                                                <  1:1333040/150‑1 (MQ=255)
                                         ccTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGtt                                                                                                              <  2:1413591/150‑1 (MQ=255)
                                          cTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttc                                                                                                             <  1:4084674/150‑1 (MQ=255)
                                          atACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttc                                                                                                             <  1:4385524/149‑1 (MQ=255)
                                           tACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttct                                                                                                            <  2:496224/150‑1 (MQ=255)
                                           tACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttct                                                                                                            <  2:3589694/150‑1 (MQ=255)
                                           tACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttct                                                                                                            <  1:2674173/150‑1 (MQ=255)
                                           tACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttct                                                                                                            >  2:1115620/1‑150 (MQ=255)
                                            aCAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCC‑GACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttc                                                                                                          <  1:3001453/150‑1 (MQ=255)
                                              aaaGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttct                                                                                                         <  2:4310230/150‑1 (MQ=255)
                                              aaaGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttct                                                                                                         <  2:2299751/150‑1 (MQ=255)
                                              aaaGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttct                                                                                                         <  1:3529414/150‑1 (MQ=255)
                                                aGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttcttc                                                                                                       >  1:3834607/1‑150 (MQ=255)
                                                aGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGttcttcttc                                                                                                       <  2:1081470/150‑1 (MQ=255)
                                                      gTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAAt                                                                                                 >  1:563213/1‑150 (MQ=255)
                                                      gTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAAt                                                                                                 <  1:3086307/150‑1 (MQ=255)
                                                      gTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAAt                                                                                                 <  1:769491/150‑1 (MQ=255)
                                                          ttCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAac                                                                                             <  2:2726460/150‑1 (MQ=255)
                                                            cAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAacac                                                                                           <  1:1156581/150‑1 (MQ=255)
                                                             aGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACAct                                                                                          >  2:4480914/1‑150 (MQ=255)
                                                             aGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACAct                                                                                          <  1:4513269/150‑1 (MQ=255)
                                                             aGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACAct                                                                                          <  1:2599415/150‑1 (MQ=255)
                                                                 aCTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCTATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACActtctt                                                                                      <  1:4307069/150‑1 (MQ=255)
                                                                  cTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCttt                                                                                     <  2:1274179/150‑1 (MQ=255)
                                                                  cTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCttt                                                                                     <  2:2328661/150‑1 (MQ=255)
                                                                      gTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATg                                                                                 >  1:4300230/1‑150 (MQ=255)
                                                                       tAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGc                                                                                >  1:1255878/1‑150 (MQ=255)
                                                                        aGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGcc                                                                               <  2:346280/150‑1 (MQ=255)
                                                                        aGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGcc                                                                               >  2:2939150/1‑150 (MQ=255)
                                                                        aGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGcc                                                                               <  2:2870409/150‑1 (MQ=255)
                                                                        aGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGcc                                                                               >  2:2134610/1‑150 (MQ=255)
                                                                         ggCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCt                                                                              >  1:3308548/1‑150 (MQ=255)
                                                                          gCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCtt                                                                             <  1:2674766/150‑1 (MQ=255)
                                                                             tcaTAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAg                                                                          <  1:2909046/148‑1 (MQ=255)
                                                                              gATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGt                                                                         <  2:3268711/150‑1 (MQ=255)
                                                                               aTAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGtt                                                                        <  2:3088005/150‑1 (MQ=255)
                                                                                 aaGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCACTCAACACTTCTTTGATGCCTTCAGTTTc                                                                      >  2:1318349/1‑150 (MQ=255)
                                                                                    cGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAg                                                                   <  2:1941609/150‑1 (MQ=255)
                                                                                      tAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCa                                                                 >  2:3121255/1‑150 (MQ=255)
                                                                                      tAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGAGTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCa                                                                 <  1:3495151/150‑1 (MQ=255)
                                                                                       aGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGACTGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCaa                                                                >  2:3445629/1‑150 (MQ=255)
                                                                                         cgcAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAAc                                                              >  1:3242411/1‑150 (MQ=255)
                                                                                          gcagcaGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACg                                                             >  1:3344855/1‑150 (MQ=255)
                                                                                               agGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACtt                                                        <  1:1300750/150‑1 (MQ=255)
                                                                                               agGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACtt                                                        <  1:1302267/150‑1 (MQ=255)
                                                                                               agGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACtt                                                        >  1:2826867/1‑150 (MQ=255)
                                                                                                  cAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGAATTTCACATAAGCTGAATGGTCTTCATCTGCAATCAAAACATCTTTGATGCCTTCAGTTTCTAGCAAACGCAcaatta                                                     >  2:1667372/1‑145 (MQ=255)
                                                                                                       cgccgcGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGcc                                                <  2:4020999/150‑1 (MQ=255)
                                                                                                        gccgcGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCt                                               <  2:4201199/150‑1 (MQ=255)
                                                                                                        gccgcGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCt                                               <  2:4201381/150‑1 (MQ=255)
                                                                                                         ccgcGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTc                                              <  2:4027834/150‑1 (MQ=255)
                                                                                                                 tttATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCgg                                      <  1:988000/150‑1 (MQ=255)
                                                                                                                  ttATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGc                                     >  1:2072320/1‑150 (MQ=255)
                                                                                                                     tGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAAt                                  <  1:31460/150‑1 (MQ=255)
                                                                                                                       ccTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGt                                <  1:1516000/150‑1 (MQ=255)
                                                                                                                       ccTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGt                                <  1:3581999/150‑1 (MQ=255)
                                                                                                                         tGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTc                              >  2:3908714/1‑150 (MQ=255)
                                                                                                                         tGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTc                              >  2:3909890/1‑150 (MQ=255)
                                                                                                                          gACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCg                             <  2:1983410/150‑1 (MQ=255)
                                                                                                                           aCGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGc                            >  1:232181/1‑150 (MQ=255)
                                                                                                                             gAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCg                          >  2:642800/1‑150 (MQ=255)
                                                                                                                               aTTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCggg                        <  2:2621706/150‑1 (MQ=255)
                                                                                                                                   ccTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGAttt                    <  1:900455/150‑1 (MQ=255)
                                                                                                                                   ccTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGAttt                    >  2:489210/1‑150 (MQ=255)
                                                                                                                                    cTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTAAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTc                   >  2:1683270/1‑150 (MQ=255)
                                                                                                                                     tGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCa                  <  2:3403676/150‑1 (MQ=255)
                                                                                                                                     tGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCa                  <  1:2893660/150‑1 (MQ=255)
                                                                                                                                        tCTATCTCACAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATg               <  2:3993617/150‑1 (MQ=255)
                                                                                                                                        tCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATg               <  1:1989542/150‑1 (MQ=255)
                                                                                                                                           aTCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATgcgc            >  2:2922753/1‑150 (MQ=255)
                                                                                                                                               cAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAAc        >  2:2110110/1‑150 (MQ=255)
                                                                                                                                                aaaGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAAct       >  2:2440041/1‑150 (MQ=255)
                                                                                                                                                   gCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAActgct    <  2:1255953/150‑1 (MQ=255)
                                                                                                                                                     gATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAAAGTTCGCCGGGATTTCAATGCGCAAACTGCTGa  >  1:2177995/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTTTGTTCATGTCAGATGCGGCATGAACGCCTGATTCGGCCTACAAAGGTTTGTAATTCAGAAACTTTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGA  >  CP009273/440644‑440942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑