Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 441,811 A→C 5.7% I104M (ATT→ATG)  yajR ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273441,8110AC 5.7% 997.8 / 44.6 327I104M (ATT→ATG) yajRputative transporter
Reads supporting (aligned to +/- strand):  ref base A (149/159);  new base C (6/13);  total (155/172)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 334 total aligned reads displayed.

CACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAAT  >  CP009273/441664‑441957
                                                                                                                                                   |                                                                                                                                                  
cACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGaataat                                                                                                                                                  >  1:1650096/1‑150 (MQ=255)
      cGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCa                                                                                                                                            <  2:4397333/150‑1 (MQ=255)
      cGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCa                                                                                                                                            <  2:2154213/150‑1 (MQ=255)
       gCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAg                                                                                                                                           >  1:2289439/1‑150 (MQ=255)
           tGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATgg                                                                                                                                       <  2:4048135/150‑1 (MQ=255)
             gCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAg                                                                                                                                     <  1:3265031/150‑1 (MQ=255)
              cataGGTAATTCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGTATAGCAGCGCCATAACGGCGTCTGCAATTGCTCCAGAGCATTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGt                                                                                                                                    <  2:2904436/147‑1 (MQ=255)
              cAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGt                                                                                                                                    >  2:648978/1‑150 (MQ=255)
              cAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGt                                                                                                                                    >  2:3509852/1‑150 (MQ=255)
              cAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGt                                                                                                                                    >  2:2705985/1‑150 (MQ=255)
               aaaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTc                                                                                                                                   >  1:2112002/1‑150 (MQ=255)
               aaaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTc                                                                                                                                   <  1:1770906/150‑1 (MQ=255)
                aaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCg                                                                                                                                  >  2:1398606/1‑150 (MQ=255)
                aaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCg                                                                                                                                  >  2:3666542/1‑150 (MQ=255)
                 aGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCgg                                                                                                                                 >  2:685471/1‑150 (MQ=255)
                  ggTAATGCCAAAGCTCACGCCGATAAACGCGATCGCTTTGGTGCGGTTTTGTTCGCGTGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGa                                                                                                                                <  2:4515666/150‑1 (MQ=255)
                   gTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGaa                                                                                                                               >  2:4431035/1‑150 (MQ=255)
                    tAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGaaa                                                                                                                              <  2:1831358/150‑1 (MQ=255)
                     aaTGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAg                                                                                                                             >  1:112532/1‑150 (MQ=255)
                      aTGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAgc                                                                                                                            <  1:2585588/150‑1 (MQ=255)
                      aTGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAgc                                                                                                                            >  2:357868/1‑150 (MQ=255)
                        gCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAgcgc                                                                                                                          >  1:2738622/1‑150 (MQ=255)
                         ccAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGACAGCGCa                                                                                                                         >  2:4367690/1‑150 (MQ=255)
                          cAAAGCTCACGCCGTTAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAg                                                                                                                        <  2:1016319/150‑1 (MQ=255)
                          cAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGACAGCGCAg                                                                                                                        >  2:3935475/1‑150 (MQ=255)
                          cAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAg                                                                                                                        <  2:1264656/150‑1 (MQ=255)
                          cAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAg                                                                                                                        >  1:597691/1‑150 (MQ=255)
                          cAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAg                                                                                                                        >  2:1674808/1‑150 (MQ=255)
                           aaaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGc                                                                                                                       >  1:885440/1‑150 (MQ=255)
                           aaaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGc                                                                                                                       <  2:493283/150‑1 (MQ=255)
                           aaaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGc                                                                                                                       >  1:3171706/1‑150 (MQ=255)
                            aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                      >  2:3133384/1‑150 (MQ=255)
                             aGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGa                                                                                                                     >  2:1900722/1‑150 (MQ=255)
                               cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                   >  2:3719871/1‑150 (MQ=255)
                               cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                   <  2:383105/150‑1 (MQ=255)
                               cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                   >  2:1457431/1‑150 (MQ=255)
                               cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                   <  2:1934532/150‑1 (MQ=255)
                                 cACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAAc                                                                                                                 <  2:1080128/150‑1 (MQ=255)
                                      cGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAc                                                                                                            <  2:2688692/150‑1 (MQ=255)
                                       gATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAcc                                                                                                           <  1:3341499/150‑1 (MQ=255)
                                       gATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAcc                                                                                                           <  1:1065623/150‑1 (MQ=255)
                                        aTAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcg                                                                                                          >  1:1373611/1‑150 (MQ=255)
                                           aaCGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcggcg                                                                                                       <  2:2648388/150‑1 (MQ=255)
                                              gCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCa                                                                                                    >  2:1515070/1‑150 (MQ=255)
                                                  tCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAaca                                                                                                <  1:992374/150‑1 (MQ=255)
                                                    gCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACAcc                                                                                              <  2:2739044/150‑1 (MQ=255)
                                                        tGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCa                                                                                          <  2:3537429/150‑1 (MQ=255)
                                                         ggTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAg                                                                                         <  1:3809565/150‑1 (MQ=255)
                                                               gTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCTTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAcc                                                                                   <  1:4229652/150‑1 (MQ=255)
                                                                ttttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCg                                                                                  <  2:4239326/150‑1 (MQ=255)
                                                                 tttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGa                                                                                 <  1:4216829/150‑1 (MQ=255)
                                                                    gTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACaa                                                                              >  2:3368584/1‑150 (MQ=255)
                                                                     ttCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAt                                                                             >  2:1790887/1‑150 (MQ=255)
                                                                       ctcgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCTGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTa                                                                           <  2:2329448/148‑1 (MQ=255)
                                                                       cgcgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTa                                                                           <  2:2329782/150‑1 (MQ=255)
                                                                         cgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAAt                                                                         <  1:1004125/150‑1 (MQ=255)
                                                                         cgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAAt                                                                         <  2:4294196/150‑1 (MQ=255)
                                                                           cgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATgg                                                                       >  2:2411655/1‑150 (MQ=255)
                                                                            gTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGt                                                                      >  2:2732805/1‑150 (MQ=255)
                                                                            gTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGt                                                                      >  1:4034322/1‑150 (MQ=255)
                                                                              gagaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGttt                                                                    <  1:1353863/150‑1 (MQ=255)
                                                                              gagaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGttt                                                                    >  2:611284/1‑150 (MQ=255)
                                                                               agaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTg                                                                   <  1:3509852/150‑1 (MQ=255)
                                                                                gaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGc                                                                  >  1:3471897/1‑150 (MQ=255)
                                                                                gaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGc                                                                  >  2:124197/1‑150 (MQ=255)
                                                                                    ggAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAcc                                                              <  2:3891401/150‑1 (MQ=255)
                                                                                    ggAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAcc                                                              >  1:3895848/1‑150 (MQ=255)
                                                                                    ggAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAcc                                                              >  1:4522881/1‑150 (MQ=255)
                                                                                     gAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCa                                                             <  1:2654535/150‑1 (MQ=255)
                                                                                          cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                        <  1:1731392/150‑1 (MQ=255)
                                                                                              gcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCg                                                    <  1:2208663/150‑1 (MQ=255)
                                                                                              gcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCg                                                    <  2:2225861/150‑1 (MQ=255)
                                                                                                 aTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaa                                                 >  2:3629135/1‑150 (MQ=255)
                                                                                                   aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                               >  1:2403733/1‑150 (MQ=255)
                                                                                                   aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                               >  1:1785373/1‑150 (MQ=255)
                                                                                                     cggcggcggCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCa                                             <  1:1457431/150‑1 (MQ=255)
                                                                                                     cggcggcggCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCa                                             <  1:3719871/150‑1 (MQ=255)
                                                                                                      ggcggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAg                                            >  1:1814869/1‑150 (MQ=255)
                                                                                                          gcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCa                                        <  1:2820547/150‑1 (MQ=255)
                                                                                                            ggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCaaa                                      >  2:14059/1‑150 (MQ=255)
                                                                                                            ggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCaaa                                      >  2:4104819/1‑150 (MQ=255)
                                                                                                               aaTTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACgg                                   >  2:729188/1‑150 (MQ=255)
                                                                                                                 ttGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGaa                                 >  1:943503/1‑150 (MQ=255)
                                                                                                                  tGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAAt                                <  2:3473855/150‑1 (MQ=255)
                                                                                                                     tCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTg                             <  1:743962/150‑1 (MQ=255)
                                                                                                                     tCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTg                             >  2:3271071/1‑150 (MQ=255)
                                                                                                                          agCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaaaa                        <  2:1358338/150‑1 (MQ=255)
                                                                                                                                 tAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTg                 <  1:914983/150‑1 (MQ=255)
                                                                                                                                 tAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTg                 <  1:4097612/150‑1 (MQ=255)
                                                                                                                                    cgcCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGTAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGt              <  1:2915917/150‑1 (MQ=255)
                                                                                                                                     gcCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTc             <  2:1165627/150‑1 (MQ=255)
                                                                                                                                      cccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCa            <  1:756342/150‑1 (MQ=255)
                                                                                                                                      cccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCa            <  1:357868/150‑1 (MQ=255)
                                                                                                                                        gggCCCAGATTAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACTCTACCGGCTGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAATCGGAATCTGTAAAACGGCCTGATTCAGa          <  2:2490083/149‑1 (MQ=255)
                                                                                                                                        cGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGa          >  2:39180/1‑150 (MQ=255)
                                                                                                                                        cGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGa          >  1:2682838/1‑150 (MQ=255)
                                                                                                                                            ccAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCAt      <  2:1226827/150‑1 (MQ=255)
                                                                                                                                               gAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaaa   <  2:2560735/150‑1 (MQ=255)
                                                                                                                                               gAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaaa   <  2:807867/150‑1 (MQ=255)
                                                                                                                                                aataagTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAAt  <  2:2738344/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAAT  >  CP009273/441664‑441957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑