Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 481,667 G→A 100% A151T (GCG→ACG)  acrR → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273481,6670GA97.0% 915.1 / 11.4 264A151T (GCG→ACG) acrRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base A (133/123);  total (135/129)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

ACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT  >  CP009273/481520‑481812
                                                                                                                                                   |                                                                                                                                                 
  agaagaACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaa                                                                                                                                               >  2:4313442/1‑150 (MQ=255)
  agaagaACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaa                                                                                                                                               >  2:499290/1‑150 (MQ=255)
   gaagaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaaa                                                                                                                                              <  2:4187265/150‑1 (MQ=255)
    aagaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGaaaa                                                                                                                                             <  2:3956257/150‑1 (MQ=255)
     agaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAAt                                                                                                                                            <  2:593306/150‑1 (MQ=255)
        aCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGtt                                                                                                                                         <  1:1741920/150‑1 (MQ=255)
         cGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTg                                                                                                                                        <  2:633289/150‑1 (MQ=255)
         cGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                        <  2:441466/150‑1 (MQ=255)
         cGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTg                                                                                                                                        >  1:2187524/1‑150 (MQ=255)
            cGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCt                                                                                                                                     <  2:1572363/150‑1 (MQ=255)
            cGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCt                                                                                                                                     <  2:3467666/150‑1 (MQ=255)
             gTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTg                                                                                                                                    >  1:4312599/1‑150 (MQ=255)
                gATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCgg                                                                                                                                 <  1:2224945/150‑1 (MQ=255)
                  ttattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAt                                                                                                                               >  1:3246603/1‑150 (MQ=255)
                    attGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAttt                                                                                                                             <  2:1347433/150‑1 (MQ=255)
                        aTGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATg                                                                                                                         <  1:529086/150‑1 (MQ=255)
                         tGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGa                                                                                                                        <  1:3822251/150‑1 (MQ=255)
                         tGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGa                                                                                                                        <  1:3822072/150‑1 (MQ=255)
                          ggAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAc                                                                                                                       <  2:1185855/150‑1 (MQ=255)
                            agaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                     >  1:2542697/1‑150 (MQ=255)
                            agaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                     >  2:868010/1‑150 (MQ=255)
                            agaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgc                                                                                                                     >  1:2753569/1‑150 (MQ=255)
                            agaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTGATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTCATGAcgc                                                                                                                     >  2:3149954/1‑150 (MQ=255)
                                tataTTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcg                                                                                                                 >  2:2968992/1‑150 (MQ=255)
                                tataTTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcg                                                                                                                 >  2:361121/1‑150 (MQ=255)
                                   atTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAATATGTTGCCTGCGGATTTAATGACGCGTCgcgc                                                                                                              >  1:2600712/1‑150 (MQ=255)
                                   atTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCgcgc                                                                                                              >  1:2601798/1‑150 (MQ=255)
                                    ttCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACAGTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgca                                                                                                             >  2:525350/1‑150 (MQ=255)
                                      ccACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgcagc                                                                                                           <  1:363836/150‑1 (MQ=255)
                                            aTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                     >  1:3311291/1‑150 (MQ=255)
                                            aTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                     >  1:159625/1‑150 (MQ=255)
                                               cGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATtattat                                                                                                  <  1:499290/150‑1 (MQ=255)
                                                gAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATg                                                                                                 >  1:2747458/1‑150 (MQ=255)
                                                 aaTTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATgc                                                                                                <  2:3599726/150‑1 (MQ=255)
                                                      gTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGc                                                                                           >  2:1304844/1‑150 (MQ=255)
                                                       tCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                          >  2:844220/1‑150 (MQ=255)
                                                        cGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCta                                                                                         >  2:2508225/1‑150 (MQ=255)
                                                         ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        >  2:899596/1‑150 (MQ=255)
                                                         ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        <  2:1444399/150‑1 (MQ=255)
                                                         ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                        >  1:3963638/1‑150 (MQ=255)
                                                          gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtata                                                                                       >  2:1681916/1‑150 (MQ=255)
                                                                     gttgtGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTg                                                                            >  2:2480724/1‑150 (MQ=255)
                                                                       tgtgCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGAt                                                                          >  2:434556/1‑150 (MQ=255)
                                                                            aaCAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaa                                                                     <  2:3143934/150‑1 (MQ=255)
                                                                             aCAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaaa                                                                    >  1:4526977/1‑150 (MQ=255)
                                                                             aCAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaaa                                                                    <  2:4361854/150‑1 (MQ=255)
                                                                                ggCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCAGGCCTGAAGGAAAACTg                                                                 >  2:4420432/1‑150 (MQ=255)
                                                                                    caACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctc                                                             <  1:1087036/150‑1 (MQ=255)
                                                                                    caACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctc                                                             <  2:1659907/150‑1 (MQ=255)
                                                                                      aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                           <  2:3211383/150‑1 (MQ=255)
                                                                                      aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                           <  1:2271830/150‑1 (MQ=255)
                                                                                        gTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTg                                                         >  1:691558/1‑150 (MQ=255)
                                                                                         tAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGc                                                        >  2:3679320/1‑150 (MQ=255)
                                                                                            tctcTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGcccc                                                     <  1:2850628/150‑1 (MQ=255)
                                                                                               ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCa                                                  <  2:723517/150‑1 (MQ=255)
                                                                                               ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCa                                                  <  2:4059851/150‑1 (MQ=255)
                                                                                               ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCa                                                  <  2:724151/150‑1 (MQ=255)
                                                                                                tgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCaa                                                 <  2:869027/150‑1 (MQ=255)
                                                                                                tgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCaa                                                 >  2:2999848/1‑150 (MQ=255)
                                                                                                  tctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATc                                               <  2:3949028/150‑1 (MQ=255)
                                                                                                  tctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATc                                               >  1:1957758/1‑150 (MQ=255)
                                                                                                   ctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                              <  1:1335937/150‑1 (MQ=255)
                                                                                                   ctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                              <  1:1916505/150‑1 (MQ=255)
                                                                                                    tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                             <  1:1534927/150‑1 (MQ=255)
                                                                                                    tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                             >  1:4057280/1‑150 (MQ=255)
                                                                                                      gAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtttt                                           <  1:2975021/150‑1 (MQ=255)
                                                                                                       aaaGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTg                                          <  1:1321634/150‑1 (MQ=255)
                                                                                                         aGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGAt                                        <  1:217424/150‑1 (MQ=255)
                                                                                                          gTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATc                                       >  1:3837669/1‑150 (MQ=255)
                                                                                                            tATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                     >  2:2304018/1‑150 (MQ=255)
                                                                                                            tATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCtt                                     <  2:3407875/150‑1 (MQ=255)
                                                                                                             aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTa                                    >  2:1916090/1‑150 (MQ=255)
                                                                                                             aTGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTa                                    <  2:1736806/150‑1 (MQ=255)
                                                                                                                aCCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaa                                 >  1:3535828/1‑150 (MQ=255)
                                                                                                                 ccGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCAGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCAGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaaa                                >  2:1727124/1‑150 (MQ=255)
                                                                                                                  cGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaaaa                               >  1:2454480/1‑150 (MQ=255)
                                                                                                                    tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaaga                             >  1:1270433/1‑150 (MQ=255)
                                                                                                                      taGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaag                           >  1:887357/1‑150 (MQ=255)
                                                                                                                       aGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGc                          >  1:154135/1‑150 (MQ=255)
                                                                                                                        gAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGcc                         <  2:2596328/150‑1 (MQ=255)
                                                                                                                         aaCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                        >  1:2339362/1‑150 (MQ=255)
                                                                                                                           cAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                      >  2:1092393/1‑150 (MQ=255)
                                                                                                                           cAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                      >  1:333102/1‑150 (MQ=255)
                                                                                                                            aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgcg                     <  2:2787650/150‑1 (MQ=255)
                                                                                                                            aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgcg                     <  2:2187524/150‑1 (MQ=255)
                                                                                                                             aaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAATAAGCCCGCGa                    >  2:2570960/1‑150 (MQ=255)
                                                                                                                             aaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGa                    >  2:274303/1‑150 (MQ=255)
                                                                                                                              aCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAt                   >  1:2177282/1‑150 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                  >  2:1551744/1‑150 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                  >  2:2225776/1‑150 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                  <  1:3595144/150‑1 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                  <  1:3595120/150‑1 (MQ=255)
                                                                                                                                 ttAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAc                >  1:1091182/1‑150 (MQ=255)
                                                                                                                                    aaaCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGtt             >  2:4134491/1‑150 (MQ=255)
                                                                                                                                     aaCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTg            <  2:1624704/150‑1 (MQ=255)
                                                                                                                                         ttGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCAGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCAt        <  2:3273808/150‑1 (MQ=255)
                                                                                                                                          tGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATc       >  1:4332017/1‑150 (MQ=255)
                                                                                                                                              ttGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc   >  2:2671171/1‑150 (MQ=255)
                                                                                                                                              ttGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc   >  2:2018043/1‑150 (MQ=255)
                                                                                                                                              ttGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc   >  1:2390514/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT  >  CP009273/481520‑481812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑