Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 530,466 C→A 93.6% F365L (TTC→TTA)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273530,4660CA93.6% 713.5 / 37.1 234F365L (TTC→TTA) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base A (96/122);  total (103/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 234 total aligned reads displayed.

GTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC  >  CP009273/530321‑530610
                                                                                                                                                 |                                                                                                                                                
gTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACa                                                                                                                                              >  2:2692320/1‑150 (MQ=255)
  aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAc                                                                                                                                            >  1:305476/1‑150 (MQ=255)
  aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAc                                                                                                                                            <  1:859250/150‑1 (MQ=255)
     gTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTg                                                                                                                                         <  1:3581224/150‑1 (MQ=255)
     gTCTCGGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTg                                                                                                                                         <  2:554960/150‑1 (MQ=255)
       ctctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGcc                                                                                                                                       <  2:1233874/150‑1 (MQ=255)
        tctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCg                                                                                                                                      <  2:3243221/150‑1 (MQ=255)
         ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     >  2:3309324/1‑150 (MQ=255)
         ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     >  1:169880/1‑150 (MQ=255)
         ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     >  1:2465812/1‑150 (MQ=255)
         ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     >  2:1100570/1‑150 (MQ=255)
         ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGACGTGTCGTAAAGAATGGGTCGCCGACTGACAGCAGCGTAAACGCACTATGCTGCGAAAAACCCACTTATACAACGTGCCgg                                                                                                                                     >  1:3219776/1‑150 (MQ=255)
           gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTg                                                                                                                                   >  2:764361/1‑150 (MQ=255)
             tGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGaa                                                                                                                                 >  1:2624218/1‑150 (MQ=255)
             tGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGaa                                                                                                                                 >  1:2624258/1‑150 (MQ=255)
                tAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAAcc                                                                                                                              <  2:3854461/150‑1 (MQ=255)
                tAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCATGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAAAGCACTTTGCTGCGCAAAACCCACTTTGACAACGTGCCGGTGAAAcc                                                                                                                              <  2:3854590/150‑1 (MQ=255)
                    gcggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAg                                                                                                                          >  2:2335770/1‑150 (MQ=255)
                     cggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGGCAACGTGCCGGTGAAACCGCAgc                                                                                                                         >  2:1440932/1‑150 (MQ=255)
                     cggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgc                                                                                                                         >  2:1833302/1‑150 (MQ=255)
                     cggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAgc                                                                                                                         <  1:821050/150‑1 (MQ=255)
                     cggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAgc                                                                                                                         <  1:820380/150‑1 (MQ=255)
                     cggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAgc                                                                                                                         <  1:1066642/150‑1 (MQ=255)
                        cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGcgcg                                                                                                                      <  1:2413703/150‑1 (MQ=255)
                        cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGcgcg                                                                                                                      >  1:3846301/1‑150 (MQ=255)
                        cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGcgcg                                                                                                                      >  1:381385/1‑150 (MQ=255)
                         gcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCgt                                                                                                                     >  1:3650856/1‑150 (MQ=255)
                         gcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCgt                                                                                                                     <  2:3748676/150‑1 (MQ=255)
                         gcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgt                                                                                                                     >  2:42748/1‑150 (MQ=255)
                              cacTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATg                                                                                                                <  1:4139294/150‑1 (MQ=255)
                                ctgctgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGaa                                                                                                              >  2:2909902/1‑150 (MQ=255)
                                     ggTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGAt                                                                                                         <  2:1889132/150‑1 (MQ=255)
                                     ggTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGAt                                                                                                         <  1:2109786/150‑1 (MQ=255)
                                     ggTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGAt                                                                                                         <  2:4140723/150‑1 (MQ=255)
                                        tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGaa                                                                                                      <  2:4318306/150‑1 (MQ=255)
                                         gAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAAc                                                                                                     <  1:2204367/150‑1 (MQ=255)
                                         gAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAAc                                                                                                     <  2:1222671/150‑1 (MQ=255)
                                           aGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaa                                                                                                   >  2:2639183/1‑150 (MQ=255)
                                             tGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAg                                                                                                 <  2:2035457/150‑1 (MQ=255)
                                             tGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAg                                                                                                 <  1:3547461/150‑1 (MQ=255)
                                              ggCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGc                                                                                                <  1:1000662/150‑1 (MQ=255)
                                                 gcAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCtt                                                                                             >  1:2793839/1‑150 (MQ=255)
                                                  cAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCttt                                                                                            <  1:255819/150‑1 (MQ=255)
                                                    ggAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTgg                                                                                          <  1:3723348/150‑1 (MQ=255)
                                                       gaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcg                                                                                       <  1:2338076/150‑1 (MQ=255)
                                                          gCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGa                                                                                    <  2:155226/150‑1 (MQ=255)
                                                           cAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt                                                                                   <  1:3889475/150‑1 (MQ=255)
                                                           cAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt                                                                                   <  1:4008865/150‑1 (MQ=255)
                                                            aaaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGagg                                                                                  >  2:2692851/1‑148 (MQ=255)
                                                            aaaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtg                                                                                  >  2:1385727/1‑150 (MQ=255)
                                                             aaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgt                                                                                 >  1:2706370/1‑150 (MQ=255)
                                                               aaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgttt                                                                               <  1:2241805/150‑1 (MQ=255)
                                                                  cGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgtttgtt                                                                            >  2:54701/1‑150 (MQ=255)
                                                                  cGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgtgtgtt                                                                            >  2:213959/1‑150 (MQ=255)
                                                                   gggTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTa                                                                           <  2:1719545/150‑1 (MQ=255)
                                                                   gggTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTa                                                                           <  1:1814915/150‑1 (MQ=255)
                                                                     gtcgtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATg                                                                         <  2:694060/150‑1 (MQ=255)
                                                                       cgtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTc                                                                       >  1:212795/1‑150 (MQ=255)
                                                                        gtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTca                                                                      >  2:3093494/1‑150 (MQ=255)
                                                                          cTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacc                                                                    <  1:1100570/150‑1 (MQ=255)
                                                                          cTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacc                                                                    <  1:3309624/150‑1 (MQ=255)
                                                                           tGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacca                                                                   >  2:1925011/1‑150 (MQ=255)
                                                                               gtgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAt                                                               >  2:322760/1‑150 (MQ=255)
                                                                                tgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAtt                                                              <  2:353230/150‑1 (MQ=255)
                                                                                tgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAtt                                                              <  2:27633/150‑1 (MQ=255)
                                                                                tgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAtt                                                              >  1:63909/1‑150 (MQ=255)
                                                                                 gtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTg                                                             >  1:555060/1‑150 (MQ=255)
                                                                                 gtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTg                                                             >  1:554690/1‑150 (MQ=255)
                                                                                   cGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGt                                                           <  1:1420396/150‑1 (MQ=255)
                                                                                   cGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGt                                                           <  2:4275800/150‑1 (MQ=255)
                                                                                      aaaGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTg                                                        <  2:1226881/150‑1 (MQ=255)
                                                                                         gAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTca                                                     >  1:2721762/1‑150 (MQ=255)
                                                                                          aaTGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTcac                                                    >  1:3329180/1‑150 (MQ=255)
                                                                                              ggTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAAt                                                <  1:2364439/150‑1 (MQ=255)
                                                                                               gTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATc                                               <  1:3301706/150‑1 (MQ=255)
                                                                                               gTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTACACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATc                                               <  1:3666919/150‑1 (MQ=255)
                                                                                                tCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCg                                              <  2:802599/150‑1 (MQ=255)
                                                                                                  gCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCt                                            <  1:656624/150‑1 (MQ=255)
                                                                                                   ccGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAGGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTg                                           >  1:2826391/1‑150 (MQ=255)
                                                                                                      aCTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCgg                                        >  1:1552332/1‑150 (MQ=255)
                                                                                                            agcagcGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACaaa                                  <  2:88216/150‑1 (MQ=255)
                                                                                                            agcagcGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACaaa                                  <  2:88300/150‑1 (MQ=255)
                                                                                                             gcagcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAt                                 <  1:1279605/150‑1 (MQ=255)
                                                                                                              cagcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtg                                <  2:1747007/150‑1 (MQ=255)
                                                                                                               agcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgc                               <  2:335141/150‑1 (MQ=255)
                                                                                                               agcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgc                               <  2:2199131/150‑1 (MQ=255)
                                                                                                                         cACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCtt                     <  1:1837330/150‑1 (MQ=255)
                                                                                                                          aCTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCTCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCttt                    >  2:2706704/1‑150 (MQ=255)
                                                                                                                          aCTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCttt                    >  2:3675904/1‑150 (MQ=255)
                                                                                                                            tttGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTaa                  >  1:3396831/1‑150 (MQ=255)
                                                                                                                            tttGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTaa                  <  1:3812768/150‑1 (MQ=255)
                                                                                                                                ctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAc              <  1:1915314/150‑1 (MQ=255)
                                                                                                                                ctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAc              >  1:517708/1‑150 (MQ=255)
                                                                                                                                 tgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAcc             <  1:2909902/150‑1 (MQ=255)
                                                                                                                                 tgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAcc             <  1:4342404/150‑1 (MQ=255)
                                                                                                                                    gcAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGcc          <  2:2053234/150‑1 (MQ=255)
                                                                                                                                        aaCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTg      >  1:3708546/1‑150 (MQ=255)
                                                                                                                                            cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATc  >  2:482091/1‑150 (MQ=255)
                                                                                                                                            cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATc  <  2:2170927/150‑1 (MQ=255)
                                                                                                                                            cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATc  <  2:3390178/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC  >  CP009273/530321‑530610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑