Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 613,755 G→T 42.0% D16Y (GAT→TAT)  fepE → regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273613,7550GT42.0% 264.4 / 315.2 264D16Y (GAT→TAT) fepEregulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base G (91/62);  new base T (56/55);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 267 total aligned reads displayed.

GTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGG  >  CP009273/613606‑613904
                                                                                                                                                     |                                                                                                                                                     
gTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCg                                                                                                                                                       >  2:3509613/1‑150 (MQ=255)
 tGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGa                                                                                                                                                      >  1:2445586/1‑150 (MQ=255)
  ggaaggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGAt                                                                                                                                                     >  1:4250275/1‑150 (MQ=255)
      ggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATc                                                                                                                                                 <  1:685843/150‑1 (MQ=255)
      ggaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATc                                                                                                                                                 <  2:3848269/150‑1 (MQ=255)
       gaaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATcc                                                                                                                                                <  1:1224691/150‑1 (MQ=255)
        aaTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCct                                                                                                                                               >  1:217566/1‑150 (MQ=255)
         aTTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCctc                                                                                                                                              >  2:1184340/1‑150 (MQ=255)
          tttACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCctct                                                                                                                                             <  1:1090900/150‑1 (MQ=255)
           ttACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTg                                                                                                                                            <  2:1559722/150‑1 (MQ=255)
             aCAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGc                                                                                                                                          <  1:3532840/150‑1 (MQ=255)
               aaaCTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGt                                                                                                                                        >  2:4115435/1‑150 (MQ=255)
               aaaCTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGt                                                                                                                                        >  2:1865947/1‑150 (MQ=255)
                 aCTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCg                                                                                                                                      <  1:2243671/150‑1 (MQ=255)
                     cAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCa                                                                                                                                  <  2:1023346/150‑1 (MQ=255)
                     cAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCa                                                                                                                                  <  2:2527172/150‑1 (MQ=255)
                         ttCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGtaa                                                                                                                              <  2:3597974/150‑1 (MQ=255)
                           cGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGtaata                                                                                                                            >  1:3122811/1‑150 (MQ=255)
                             gATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTaataat                                                                                                                          <  2:190697/150‑1 (MQ=255)
                             gATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTaataat                                                                                                                          <  1:2849746/150‑1 (MQ=255)
                               tAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCACAGTAATCATGa                                                                                                                        >  2:3676820/1‑150 (MQ=255)
                               tAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCCTCGCACAGTAATAATGa                                                                                                                        >  2:3676734/1‑150 (MQ=255)
                                 aTTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGaaa                                                                                                                      <  2:3702068/150‑1 (MQ=255)
                                 aTTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGaaa                                                                                                                      >  2:277180/1‑150 (MQ=255)
                                 aTTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGaaa                                                                                                                      >  2:2382202/1‑150 (MQ=255)
                                  ttATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAAt                                                                                                                     >  2:1078583/1‑150 (MQ=255)
                                  ttATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAAt                                                                                                                     >  1:1826407/1‑150 (MQ=255)
                                   tATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAAtt                                                                                                                    >  1:3597286/1‑150 (MQ=255)
                                   tATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAAtc                                                                                                                    <  1:1236113/150‑2 (MQ=255)
                                    aTCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTg                                                                                                                   >  1:2002920/1‑150 (MQ=255)
                                    aTCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTg                                                                                                                   >  1:2003067/1‑150 (MQ=255)
                                    aTCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTg                                                                                                                   >  2:1726814/1‑150 (MQ=255)
                                      cACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGAt                                                                                                                 <  2:2280083/150‑1 (MQ=255)
                                          aaCTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTAc                                                                                                             >  2:2337199/1‑150 (MQ=255)
                                          aaCTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTAc                                                                                                             <  2:1292356/150‑1 (MQ=255)
                                           aCTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACt                                                                                                            <  1:981523/150‑1 (MQ=255)
                                             tGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTta                                                                                                          >  1:3339626/1‑150 (MQ=255)
                                               gTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaat                                                                                                        >  1:2209826/1‑150 (MQ=255)
                                                 aaaGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaatct                                                                                                      >  1:3774438/1‑150 (MQ=255)
                                                  aaGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaatcta                                                                                                     >  1:760307/1‑150 (MQ=255)
                                                    gTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaatctaat                                                                                                   <  2:121452/150‑1 (MQ=255)
                                                     tGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTtaatctaatc                                                                                                  >  2:477056/1‑150 (MQ=255)
                                                      gAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTaatctaatct                                                                                                 >  1:1841014/1‑150 (MQ=255)
                                                         cgcCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAg                                                                                              >  2:3457157/1‑150 (MQ=255)
                                                          gcCGTGGATATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGt                                                                                             <  1:1972710/150‑1 (MQ=255)
                                                          gcCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGt                                                                                             <  1:3146877/150‑1 (MQ=255)
                                                          gcCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGt                                                                                             <  1:3146931/150‑1 (MQ=255)
                                                            cGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGttt                                                                                           >  1:4265727/1‑150 (MQ=255)
                                                            cGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGttt                                                                                           >  1:4265766/1‑150 (MQ=255)
                                                            cGTGGAGATTTCCCCCTATCTGACTCATTTTCAGGTTATACCCCATGTCATCACTGATTATTAAACAGGGAAGTGACGCTCATTTTCCATATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAtttt                                                                                           <  2:4372802/150‑1 (MQ=255)
                                                             gTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGtttt                                                                                          <  1:2531562/150‑1 (MQ=255)
                                                             gTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCTAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGtttt                                                                                          >  1:318957/1‑150 (MQ=255)
                                                               tgagaTTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTAt                                                                                        <  2:2713059/149‑1 (MQ=255)
                                                                  gaTTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGc                                                                                     <  1:1458083/150‑1 (MQ=255)
                                                                   aTTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCg                                                                                    >  1:1401475/1‑150 (MQ=255)
                                                                        ccccTATTGGACTCATTTTCAGGTTATACCCCATGTCATCAGTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCa                                                                               >  1:2922598/1‑150 (MQ=255)
                                                                        ccccTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCa                                                                               >  1:650935/1‑150 (MQ=255)
                                                                        ccccTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCa                                                                               >  1:728493/1‑150 (MQ=255)
                                                                               tGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAAc                                                                        >  1:2780103/1‑150 (MQ=255)
                                                                                   cTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTc                                                                    >  1:4141377/1‑150 (MQ=255)
                                                                                   cTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTc                                                                    >  1:4418888/1‑150 (MQ=255)
                                                                                   cTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTc                                                                    >  1:4141383/1‑150 (MQ=255)
                                                                                   cTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTc                                                                    <  1:578929/150‑1 (MQ=255)
                                                                                     cATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCAt                                                                  <  1:4172189/150‑1 (MQ=255)
                                                                                         ttCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCg                                                              <  1:3788694/150‑1 (MQ=255)
                                                                                           cAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGt                                                            >  2:1698620/1‑150 (MQ=255)
                                                                                            aGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTc                                                           <  1:3993299/150‑1 (MQ=255)
                                                                                                tataCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGttt                                                       <  2:4233716/150‑1 (MQ=255)
                                                                                                tataCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGttt                                                       >  1:3007645/1‑150 (MQ=255)
                                                                                                  taCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGttttt                                                     <  1:157427/150‑1 (MQ=255)
                                                                                                  taCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGttttt                                                     <  1:3584586/150‑1 (MQ=255)
                                                                                                  taCCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGttttt                                                     <  1:3509613/150‑1 (MQ=255)
                                                                                                       cATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGtt                                                >  1:1319056/1‑150 (MQ=255)
                                                                                                        aTGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGACATGGCGGTCGTTTTTGCGttt                                               >  1:3027681/1‑150 (MQ=255)
                                                                                                          gTCATCACTGAATATTAAACAGGGAAGTCACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGAAATAATGAAATTGATTTACTTAATATAATCTCAGTTTTATGGCGGGCCAAAAAAAAGGTCATGACGGTCGTTTTTGCGttttc                                             >  2:2058519/1‑148 (MQ=255)
                                                                                                           tcatcaCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGGCATGGCGGTCGTTTTTGCGTTTGcc                                            >  1:2071948/1‑150 (MQ=255)
                                                                                                               caCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTgcg                                        >  1:3417952/1‑150 (MQ=255)
                                                                                                               caCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTgcg                                        >  1:4296012/1‑150 (MQ=255)
                                                                                                               caCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTgcg                                        <  1:2271777/150‑1 (MQ=255)
                                                                                                                aCTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTgcgc                                       <  2:645381/150‑1 (MQ=255)
                                                                                                                 cTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCa                                      >  2:2331186/1‑150 (MQ=255)
                                                                                                                  tGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAg                                     >  1:1179085/1‑150 (MQ=255)
                                                                                                                  tGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAg                                     <  2:3312628/150‑1 (MQ=255)
                                                                                                                     atatTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCt                                  >  1:4492187/1‑150 (MQ=255)
                                                                                                                     atatTAAACAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCt                                  <  1:544033/150‑1 (MQ=255)
                                                                                                                        ttAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGc                               <  2:3650710/150‑1 (MQ=255)
                                                                                                                           aaCAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGa                            >  2:2099556/1‑150 (MQ=255)
                                                                                                                           aaCAGGGAAGTGACGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGa                            <  1:1039682/150‑1 (MQ=255)
                                                                                                                                 gAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCtt                      >  2:146929/1‑150 (MQ=255)
                                                                                                                                 gAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCtt                      >  2:146850/1‑150 (MQ=255)
                                                                                                                                   aGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTc                    >  2:836100/1‑150 (MQ=255)
                                                                                                                                      gACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTAATc                 >  2:3623863/1‑150 (MQ=255)
                                                                                                                                       aCGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATcc                >  2:2183363/1‑150 (MQ=255)
                                                                                                                                        cGCTCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATATCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCt               <  2:3043135/150‑1 (MQ=255)
                                                                                                                                        cGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCt               >  1:3291777/1‑150 (MQ=255)
                                                                                                                                           tCATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTTCCTGCGCAGGCTTGCTGATCTCTTTCATCCAGcc            >  1:382059/1‑150 (MQ=255)
                                                                                                                                            cATTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCg           <  2:512484/150‑1 (MQ=255)
                                                                                                                                             aTTTTCCCTATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGc          >  2:1629312/1‑150 (MQ=255)
                                                                                                                                                ttCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTAATCCTGCCGCaaa       >  1:3288955/1‑150 (MQ=255)
                                                                                                                                                     gATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATgg  <  2:295125/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGTCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAAAAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATCCTGCCGCAAAAATGG  >  CP009273/613606‑613904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑