Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 623,935 C→A 6.9% I262I (ATC→ATA)  entB → isochorismatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273623,9350CA 6.9% 920.3 / 29.4 246I262I (ATC→ATA) entBisochorismatase
Reads supporting (aligned to +/- strand):  ref base C (89/140);  new base A (11/6);  total (100/146)
Fisher's exact test for biased strand distribution p-value = 4.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 256 total aligned reads displayed.

GCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTT  >  CP009273/623786‑624084
                                                                                                                                                     |                                                                                                                                                     
gCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATc                                                                                                                                                       <  1:501197/150‑1 (MQ=255)
gCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCAATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATc                                                                                                                                                       <  1:33965/150‑1 (MQ=255)
    gCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACt                                                                                                                                                   <  2:2428095/150‑1 (MQ=255)
     cAAAGCGGCGCTGCGTGAGGTTATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACtt                                                                                                                                                  <  1:17877/150‑1 (MQ=255)
     cAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACtt                                                                                                                                                  <  2:2597051/150‑1 (MQ=255)
      aaaGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACttt                                                                                                                                                 >  2:3959229/1‑150 (MQ=255)
         gcggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTc                                                                                                                                              <  1:2709293/150‑1 (MQ=255)
           ggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCAt                                                                                                                                            >  1:1354003/1‑150 (MQ=255)
           ggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCAt                                                                                                                                            >  1:2094333/1‑150 (MQ=255)
               cTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTg                                                                                                                                        >  1:1140968/1‑150 (MQ=255)
               cTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTg                                                                                                                                        <  2:3213418/150‑1 (MQ=255)
                   gTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGa                                                                                                                                    >  1:3818664/1‑150 (MQ=255)
                     gAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGaaa                                                                                                                                  >  2:3383343/1‑150 (MQ=255)
                       ggTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGaaaaa                                                                                                                                <  2:3793455/150‑1 (MQ=255)
                       ggTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGATGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGaaaaa                                                                                                                                <  2:3798292/150‑1 (MQ=255)
                         tGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAcc                                                                                                                              <  1:4356933/150‑1 (MQ=255)
                         tGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAcc                                                                                                                              <  2:858217/150‑1 (MQ=255)
                          gATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAccc                                                                                                                             >  2:3862348/1‑150 (MQ=255)
                          gATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAccc                                                                                                                             <  1:4074355/150‑1 (MQ=255)
                           aTCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCg                                                                                                                            <  1:245792/150‑1 (MQ=255)
                                 ccGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATc                                                                                                                      <  2:2992047/150‑1 (MQ=255)
                                  cGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCg                                                                                                                     <  2:597965/150‑1 (MQ=255)
                                   gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGa                                                                                                                    <  1:2627488/150‑1 (MQ=255)
                                   gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGa                                                                                                                    >  2:3234345/1‑150 (MQ=255)
                                   gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGa                                                                                                                    >  2:4537240/1‑150 (MQ=255)
                                        tGGCCGACTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCt                                                                                                               <  2:2238628/150‑1 (MQ=255)
                                           actaGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCtggt                                                                                                            >  2:3399151/4‑150 (MQ=255)
                                             gAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATAACGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCtggtgg                                                                                                          >  2:659121/1‑150 (MQ=255)
                                                  cGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCt                                                                                                     >  2:2957896/1‑150 (MQ=255)
                                                   gATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTa                                                                                                    <  1:2756543/150‑1 (MQ=255)
                                                       aaCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTActct                                                                                                <  2:1348157/150‑1 (MQ=255)
                                                       aaCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTActct                                                                                                <  2:496702/150‑1 (MQ=255)
                                                       aaCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTActct                                                                                                <  2:1348565/150‑1 (MQ=255)
                                                          cGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTccc                                                                                             <  1:1240448/150‑1 (MQ=255)
                                                           gTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATTGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCcg                                                                                            >  1:3692482/1‑150 (MQ=255)
                                                             tCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCcgcg                                                                                          >  1:1851234/1‑150 (MQ=255)
                                                               gATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAg                                                                                        <  1:1084718/150‑1 (MQ=255)
                                                                 tGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGt                                                                                      <  2:2015045/150‑1 (MQ=255)
                                                                  gacgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTg                                                                                     <  1:1587274/150‑1 (MQ=255)
                                                                  gacgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTg                                                                                     <  1:415359/150‑1 (MQ=255)
                                                                   acgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGa                                                                                    <  1:3445103/150‑1 (MQ=255)
                                                                    cgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGaa                                                                                   <  1:4296740/150‑1 (MQ=255)
                                                                       cAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAaata                                                                                >  2:4252254/1‑150 (MQ=255)
                                                                         aCCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAaataat                                                                              <  2:1370661/150‑1 (MQ=255)
                                                                          ccTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATg                                                                             >  2:3896849/1‑150 (MQ=255)
                                                                          ccTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATg                                                                             <  1:990073/150‑1 (MQ=255)
                                                                           cTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATgg                                                                            <  2:4263268/150‑1 (MQ=255)
                                                                            tGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATggc                                                                           >  2:954882/1‑149 (MQ=255)
                                                                             gATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAt                                                                          <  1:2897136/150‑1 (MQ=255)
                                                                              aTCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAtt                                                                         >  1:525091/1‑150 (MQ=255)
                                                                              aTCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAtt                                                                         >  1:524956/1‑150 (MQ=255)
                                                                                  aCTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAg                                                                     <  1:3190707/150‑1 (MQ=255)
                                                                                   cTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGc                                                                    >  1:3226627/1‑150 (MQ=255)
                                                                                     aCGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCgg                                                                  <  1:452220/150‑1 (MQ=255)
                                                                                      cGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGt                                                                 >  1:3323986/1‑150 (MQ=255)
                                                                                       ggTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTa                                                                >  1:4345283/1‑150 (MQ=255)
                                                                                       ggTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTa                                                                <  2:191216/150‑1 (MQ=255)
                                                                                       ggTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTa                                                                <  2:189291/150‑1 (MQ=255)
                                                                                         tCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaa                                                              >  2:1597752/1‑150 (MQ=255)
                                                                                           tGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaaaa                                                            <  1:310688/150‑1 (MQ=255)
                                                                                           tGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTaaaaa                                                            <  1:3029715/150‑1 (MQ=255)
                                                                                            ggATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAAt                                                           <  2:2312334/150‑1 (MQ=255)
                                                                                               ttCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTc                                                        <  1:1701682/150‑1 (MQ=255)
                                                                                               ttCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTc                                                        <  1:3959229/150‑1 (MQ=255)
                                                                                                     gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTa                                                  <  1:3286807/150‑1 (MQ=255)
                                                                                                     gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTa                                                  >  1:2602071/1‑150 (MQ=255)
                                                                                                      cgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTaa                                                 <  1:3603845/150‑1 (MQ=255)
                                                                                                        cATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAAcc                                               <  2:3027593/150‑1 (MQ=255)
                                                                                                         atgatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCg                                              <  1:1962340/150‑1 (MQ=255)
                                                                                                         atgatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCg                                              <  1:4208121/150‑1 (MQ=255)
                                                                                                         atgatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCg                                              <  1:2339851/150‑1 (MQ=255)
                                                                                                         atgatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCg                                              >  1:1473527/1‑150 (MQ=255)
                                                                                                             tgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGgcgc                                          <  1:3435282/150‑1 (MQ=255)
                                                                                                               gcgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAg                                        <  2:1224795/150‑1 (MQ=255)
                                                                                                                  cTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTa                                     >  2:2198657/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    <  2:2812366/150‑1 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    >  2:3526458/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTCCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    >  2:1696073/1‑150 (MQ=255)
                                                                                                                    ggcggcGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaaa                                   >  1:4222034/1‑150 (MQ=255)
                                                                                                                       ggcgCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGt                                <  2:1273702/150‑1 (MQ=255)
                                                                                                                        gcgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTa                               <  2:659442/150‑1 (MQ=255)
                                                                                                                          gcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATc                             <  2:3122351/150‑1 (MQ=255)
                                                                                                                          gcgcTGGCGCAAAGTGCATGGTGATATCGACTGTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATc                             >  2:1067633/1‑150 (MQ=255)
                                                                                                                           cgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCg                            <  2:3756820/150‑1 (MQ=255)
                                                                                                                              tGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAATATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCt                         >  2:4274969/1‑150 (MQ=255)
                                                                                                                                   cAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGcc                    <  1:2957896/150‑1 (MQ=255)
                                                                                                                                    aaaGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCa                   >  1:1310716/1‑150 (MQ=255)
                                                                                                                                    aaaGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCa                   >  1:2777683/1‑150 (MQ=255)
                                                                                                                                         gCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgcg              >  1:1422963/1‑150 (MQ=255)
                                                                                                                                          cATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgcgc             >  1:2862250/1‑150 (MQ=255)
                                                                                                                                           aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCt            >  2:3102358/1‑150 (MQ=255)
                                                                                                                                           aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCt            <  1:624399/150‑1 (MQ=255)
                                                                                                                                            tGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTg           <  2:1473527/150‑1 (MQ=255)
                                                                                                                                              gTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTCCGCCACGGCGCTGGc         >  2:1208886/1‑150 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     >  1:2265726/1‑150 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     >  2:4433673/1‑150 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     >  1:2265722/1‑150 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     >  2:2287625/1‑150 (MQ=255)
                                                                                                                                                     cGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttgtt  >  2:3365127/1‑150 (MQ=255)
                                                                                                                                                     cGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttgtt  <  1:684095/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTT  >  CP009273/623786‑624084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑