Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 55,590 G→T 91.9% T507K (ACG→AAG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927355,5900GT91.9% 688.1 / 53.7 247T507K (ACG→AAG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base G (3/17);  new base T (95/132);  total (98/149)
Fisher's exact test for biased strand distribution p-value = 1.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

Only 100 of 252 total aligned reads displayed.

TTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTG  >  CP009273/55443‑55735
                                                                                                                                                   |                                                                                                                                                 
 tAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCtttt                                                                                                                                                <  2:2846815/150‑1 (MQ=255)
     ggAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGt                                                                                                                                            <  2:2241393/150‑1 (MQ=255)
       aGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtat                                                                                                                                          <  2:115680/150‑1 (MQ=255)
        ggCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGtata                                                                                                                                         >  2:2765378/1‑150 (MQ=255)
         gCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATaa                                                                                                                                        <  1:1008753/150‑1 (MQ=255)
           aaTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAcc                                                                                                                                      >  1:572936/1‑150 (MQ=255)
           aaTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAcc                                                                                                                                      >  1:77479/1‑150 (MQ=255)
           aaTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAcc                                                                                                                                      >  2:1093436/1‑150 (MQ=255)
           aaTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAcc                                                                                                                                      >  2:2799055/1‑150 (MQ=255)
            aTACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAAccc                                                                                                                                     >  1:2038937/1‑150 (MQ=255)
               cGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAAAACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGa                                                                                                                                  >  1:2923223/1‑150 (MQ=255)
                ggTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAg                                                                                                                                 <  1:3390829/150‑1 (MQ=255)
                 gTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGc                                                                                                                                <  2:1319746/150‑1 (MQ=255)
                   cAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCa                                                                                                                              <  1:4481577/150‑1 (MQ=255)
                    aaGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAg                                                                                                                             >  1:24084/1‑150 (MQ=255)
                       aCCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTTGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCAt                                                                                                                          <  2:2874266/150‑1 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                    >  1:2551278/1‑150 (MQ=255)
                             gTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCat                                                                                                                    >  1:3628822/1‑150 (MQ=255)
                              tAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCata                                                                                                                   <  1:1177050/150‑1 (MQ=255)
                               aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCatat                                                                                                                  <  2:1652651/150‑1 (MQ=255)
                               aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCatat                                                                                                                  >  2:3704535/1‑150 (MQ=255)
                               aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCatat                                                                                                                  >  2:1074878/1‑150 (MQ=255)
                               aaGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCatat                                                                                                                  >  1:2838385/1‑150 (MQ=255)
                                aGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATc                                                                                                                 <  2:3846614/150‑1 (MQ=255)
                                 gTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcg                                                                                                                >  2:2125650/1‑150 (MQ=255)
                                 gTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATcg                                                                                                                <  1:2032822/150‑1 (MQ=255)
                                      gTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTc                                                                                                           <  1:3072188/150‑1 (MQ=255)
                                      gTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTc                                                                                                           <  1:3072156/150‑1 (MQ=255)
                                        cccGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCaa                                                                                                         >  1:1553496/1‑150 (MQ=255)
                                        cccGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCaa                                                                                                         >  1:1555014/1‑150 (MQ=255)
                                          cGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAg                                                                                                       <  2:2426870/150‑1 (MQ=255)
                                            gAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGAc                                                                                                     <  2:3546839/150‑1 (MQ=255)
                                             aaCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGAcc                                                                                                    <  1:2215540/150‑1 (MQ=255)
                                             aaCAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGAcc                                                                                                    <  1:1344919/150‑1 (MQ=255)
                                               cAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAt                                                                                                  <  1:2429458/150‑1 (MQ=255)
                                                  gCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtttt                                                                                               <  2:67739/150‑1 (MQ=255)
                                                  gCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtttt                                                                                               <  2:905702/150‑1 (MQ=255)
                                                    cAGAGTAGTCAGATTTCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGc                                                                                             <  1:3981201/150‑1 (MQ=255)
                                                    cAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGc                                                                                             <  1:3785638/150‑1 (MQ=255)
                                                    cAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGc                                                                                             <  1:2492067/150‑1 (MQ=255)
                                                    cAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGc                                                                                             <  1:3784816/150‑1 (MQ=255)
                                                      gagTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCg                                                                                           >  1:1692336/1‑150 (MQ=255)
                                                       agtagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATGTTGCCGt                                                                                          >  2:4266323/1‑150 (MQ=255)
                                                        gtagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTc                                                                                         <  1:1713306/150‑1 (MQ=255)
                                                        gtagtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTc                                                                                         <  1:2496943/150‑1 (MQ=255)
                                                          agtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                                       <  1:833518/150‑1 (MQ=255)
                                                           gtCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAAc                                                                                      <  1:4219477/150‑1 (MQ=255)
                                                            tCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACt                                                                                     <  1:2508305/150‑1 (MQ=255)
                                                              aGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACttt                                                                                   <  1:528585/150‑1 (MQ=255)
                                                              aGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACttt                                                                                   >  1:3043504/1‑150 (MQ=255)
                                                                 ttGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGaa                                                                                <  1:446664/150‑1 (MQ=255)
                                                                      gcagAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCg                                                                           <  1:1236592/150‑1 (MQ=255)
                                                                          agaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                                       <  2:1263481/150‑1 (MQ=255)
                                                                           gaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAtt                                                                      >  1:2761964/1‑150 (MQ=255)
                                                                           gaCGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGAACATTTTGCCGTCAACTTTGAATTGCGGCAtt                                                                      >  1:2762005/1‑150 (MQ=255)
                                                                                   gTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGtt                                                              <  1:2263204/150‑1 (MQ=255)
                                                                                    tAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTa                                                             >  1:2878762/1‑150 (MQ=255)
                                                                                     aGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTaa                                                            <  2:3302233/150‑1 (MQ=255)
                                                                                     aGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTaa                                                            >  1:1919803/1‑150 (MQ=255)
                                                                                      gTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAAc                                                           <  1:2291363/150‑1 (MQ=255)
                                                                                       ttATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACg                                                          <  1:624329/150‑1 (MQ=255)
                                                                                         ataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGa                                                        >  2:1749672/1‑150 (MQ=255)
                                                                                          taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAt                                                       <  2:4138985/150‑1 (MQ=255)
                                                                                           aGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAtt                                                      <  1:3604813/150‑1 (MQ=255)
                                                                                           aGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAtt                                                      <  1:4120537/150‑1 (MQ=255)
                                                                                            gATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTc                                                     <  2:3940416/150‑1 (MQ=255)
                                                                                              tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGt                                                   <  2:2230003/150‑1 (MQ=255)
                                                                                                tCGCTCTGATCGCGATACGGCACGTACAAAGACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTcc                                                 <  2:3637523/150‑1 (MQ=255)
                                                                                                 cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                                >  2:1140400/1‑150 (MQ=255)
                                                                                                 cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                                <  1:2767176/150‑1 (MQ=255)
                                                                                                 cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                                >  2:1778016/1‑150 (MQ=255)
                                                                                                 cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGGTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                                                <  1:4282584/150‑1 (MQ=255)
                                                                                                   ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGc                                              >  2:551122/1‑150 (MQ=255)
                                                                                                   ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGc                                              >  2:4542608/1‑150 (MQ=255)
                                                                                                   ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGc                                              >  1:2106820/1‑150 (MQ=255)
                                                                                                     ctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGcgt                                            >  2:2636428/1‑148 (MQ=255)
                                                                                                      tGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTg                                           >  1:2753613/1‑150 (MQ=255)
                                                                                                         tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTc                                        <  2:1475563/150‑1 (MQ=255)
                                                                                                          cgcgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                                       >  2:404156/1‑150 (MQ=255)
                                                                                                           gcgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgt                                      <  2:2933110/150‑1 (MQ=255)
                                                                                                               tACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGttt                                  <  2:2038937/150‑1 (MQ=255)
                                                                                                                       gTACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATGTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt                          <  2:1923195/150‑1 (MQ=255)
                                                                                                                        tACAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta                         >  2:120399/1‑150 (MQ=255)
                                                                                                                         aCAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtat                        <  1:672695/150‑1 (MQ=255)
                                                                                                                          cAAATACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtata                       <  1:2133312/150‑1 (MQ=255)
                                                                                                                           aaaTACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAc                      >  2:2509883/1‑150 (MQ=255)
                                                                                                                              tACTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAg                   <  2:4345988/150‑1 (MQ=255)
                                                                                                                                cTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTc                 <  2:303908/150‑1 (MQ=255)
                                                                                                                                cTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTc                 <  2:77479/150‑1 (MQ=255)
                                                                                                                                cTGCGCGCGCGGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTc                 <  2:1555014/150‑1 (MQ=255)
                                                                                                                                     cgcgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGAt            <  1:2154752/150‑1 (MQ=255)
                                                                                                                                       cgcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTg          <  2:2699665/150‑1 (MQ=255)
                                                                                                                                        gcgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTgg         <  2:3678815/150‑1 (MQ=255)
                                                                                                                                         cgGTTCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGt        <  2:2601022/150‑1 (MQ=255)
                                                                                                                                           gTTCCAGCTTTTGGGTATACCCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGttt      <  2:2469610/150‑1 (MQ=255)
                                                                                                                                            ttCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTTAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTg     >  2:811523/1‑150 (MQ=255)
                                                                                                                                            ttCCAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTg     >  2:811541/1‑150 (MQ=255)
                                                                                                                                              ccAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCt   <  2:1225728/150‑1 (MQ=255)
                                                                                                                                              ccAGCTTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCt   <  1:187720/150‑1 (MQ=255)
                                                                                                                                               cAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTg  >  2:3620087/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGGTCCCGGAACAGGCCAGAGTAGTCAGATTGCAGCAGAGACGAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACCAGTCAAGATTGGTTTGCTG  >  CP009273/55443‑55735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑