Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 706,479 C→A 51.7% R148L (CGT→CTT)  fldA ← flavodoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273706,4790CA51.7% 77.5 / inf 284R148L (CGT→CTT) fldAflavodoxin 1
Reads supporting (aligned to +/- strand):  ref base C (64/73);  new base A (64/83);  total (128/156)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 285 total aligned reads displayed.

AATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGC  >  CP009273/706330‑706626
                                                                                                                                                     |                                                                                                                                                   
 attattTTGATTGGCATGATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACg                                                                                                                                                    >  1:2819344/1‑150 (MQ=255)
   tattTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGt                                                                                                                                                  <  2:4502229/150‑1 (MQ=255)
   tattTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGt                                                                                                                                                  >  2:2876290/1‑150 (MQ=255)
       ttGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTtc                                                                                                                                              <  1:2870077/150‑1 (MQ=255)
       ttGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTtc                                                                                                                                              <  2:1333849/150‑1 (MQ=255)
         gATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTtcgt                                                                                                                                            >  1:2693911/1‑150 (MQ=255)
             ggCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGAt                                                                                                                                        >  2:2747875/1‑150 (MQ=255)
             ggCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGAt                                                                                                                                        <  1:2715030/150‑1 (MQ=255)
              gCATTATCTATTAATACGGAGTAGACATGAGTATACGCCGCATCACATCAGGCATTGAGTATTTCGTCGAGATGCAACTCTTCATAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATa                                                                                                                                       <  1:2331787/150‑1 (MQ=255)
                aTTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGc                                                                                                                                     >  2:4374701/1‑150 (MQ=255)
                    tCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGa                                                                                                                                 <  2:3467599/150‑1 (MQ=255)
                     cTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGAc                                                                                                                                >  2:2222025/1‑150 (MQ=255)
                      tATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGAcc                                                                                                                               <  1:3803975/150‑1 (MQ=255)
                       aTTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCg                                                                                                                              >  2:676003/1‑150 (MQ=255)
                           aTACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACaa                                                                                                                          >  2:4016182/1‑150 (MQ=255)
                            tACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACaaa                                                                                                                         >  2:119311/1‑150 (MQ=255)
                            tACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACaaa                                                                                                                         >  2:1579124/1‑150 (MQ=255)
                             aCGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAg                                                                                                                        <  1:4271447/150‑1 (MQ=255)
                             aCGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAg                                                                                                                        <  1:27771/150‑1 (MQ=255)
                               ggCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTg                                                                                                                      <  1:2220102/150‑1 (MQ=255)
                               ggCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTg                                                                                                                      >  2:2154561/1‑150 (MQ=255)
                                 cGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGgt                                                                                                                    <  1:1138137/150‑1 (MQ=255)
                                  gTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGgtc                                                                                                                   <  1:3469719/150‑1 (MQ=255)
                                     gACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGgtcgtc                                                                                                                <  1:593235/150‑1 (MQ=255)
                                        aTGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTCCACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATc                                                                                                             >  1:4358244/1‑150 (MQ=255)
                                          gAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTg                                                                                                           <  1:4315771/150‑1 (MQ=255)
                                          gAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTg                                                                                                           <  1:616869/150‑1 (MQ=255)
                                           aGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGc                                                                                                          >  2:4248845/1‑150 (MQ=255)
                                           aGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGc                                                                                                          >  2:1387563/1‑150 (MQ=255)
                                            gTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGcc                                                                                                         <  1:3827416/150‑1 (MQ=255)
                                            gTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGcc                                                                                                         >  1:3276370/1‑150 (MQ=255)
                                             tCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCa                                                                                                        <  2:3616179/150‑1 (MQ=255)
                                             tCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCa                                                                                                        >  1:2342687/1‑150 (MQ=255)
                                                    cgcATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCttt                                                                                                 <  1:1625404/150‑1 (MQ=255)
                                                     gcATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCtttt                                                                                                >  2:492364/1‑150 (MQ=255)
                                                      catcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTg                                                                                               <  2:3503698/150‑1 (MQ=255)
                                                        tcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGAt                                                                                             <  2:4078606/150‑1 (MQ=255)
                                                        tcacatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGAt                                                                                             <  2:863327/150‑1 (MQ=255)
                                                          acatcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGc                                                                                           >  2:1011458/1‑150 (MQ=255)
                                                            atcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCtt                                                                                         >  2:3599224/1‑150 (MQ=255)
                                                            atcaGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCtt                                                                                         >  2:1013320/1‑150 (MQ=255)
                                                               aGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGa                                                                                      <  1:1977460/150‑1 (MQ=255)
                                                                ggCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGaa                                                                                     >  2:860513/1‑150 (MQ=255)
                                                                   aTTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATg                                                                                  >  2:659479/1‑150 (MQ=255)
                                                                       agaATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAg                                                                              >  1:1535842/1‑150 (MQ=255)
                                                                        gaATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGc                                                                             <  2:2311655/150‑1 (MQ=255)
                                                                        gaATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGc                                                                             <  2:2003957/150‑1 (MQ=255)
                                                                         aaTTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGcc                                                                            <  2:3078652/150‑1 (MQ=255)
                                                                           tttCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCg                                                                          <  1:4177873/150‑1 (MQ=255)
                                                                              cgtcgAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGGTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAg                                                                       <  2:3000134/150‑1 (MQ=255)
                                                                                 cgAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTg                                                                    <  1:3617597/150‑1 (MQ=255)
                                                                                   agaTGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGc                                                                  >  1:796190/1‑150 (MQ=255)
                                                                                     aTGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCa                                                                >  1:379812/1‑150 (MQ=255)
                                                                                       gCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGt                                                              >  1:2404436/1‑150 (MQ=255)
                                                                                          aCTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGAc                                                           >  1:133108/1‑150 (MQ=255)
                                                                                           ctctTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGAcc                                                          <  2:3961830/150‑1 (MQ=255)
                                                                                           ctctTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGAcc                                                          >  1:2201235/1‑150 (MQ=255)
                                                                                            tctTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCa                                                         <  1:2320842/150‑1 (MQ=255)
                                                                                             ctTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCaa                                                        <  2:1856344/150‑1 (MQ=255)
                                                                                               tCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACg                                                      <  1:2785687/150‑1 (MQ=255)
                                                                                               tCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACg                                                      <  1:2785635/150‑1 (MQ=255)
                                                                                                cAGAAATCTGTTTAACCCATTTTTCTACACGTTCGGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGa                                                     >  2:3436424/1‑150 (MQ=255)
                                                                                                 aGAAATCTGTTTAACCTATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGAt                                                    >  1:3551942/1‑150 (MQ=255)
                                                                                                 aGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGAt                                                    >  1:3854338/1‑150 (MQ=255)
                                                                                                   aaaTCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATgg                                                  >  1:2707667/1‑150 (MQ=255)
                                                                                                     aTCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGtt                                                <  2:1430211/150‑1 (MQ=255)
                                                                                                         gTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCAc                                            >  2:535292/1‑150 (MQ=255)
                                                                                                         gTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTGTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCAc                                            >  2:1109931/1‑150 (MQ=255)
                                                                                                          tttAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCAcc                                           <  2:2819339/150‑1 (MQ=255)
                                                                                                              aCCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCCGTTGGCCAGTGACCAACGATGGTTGCACcgcgc                                       >  2:2654104/1‑150 (MQ=255)
                                                                                                                ccATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCgg                                     >  1:2532366/1‑150 (MQ=255)
                                                                                                                    ttttCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCa                                 >  1:1025429/1‑150 (MQ=255)
                                                                                                                    ttttCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCa                                 <  2:2017077/150‑1 (MQ=255)
                                                                                                                     tttCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCaa                                <  1:2048011/150‑1 (MQ=255)
                                                                                                                      ttCTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAat                               <  1:4473207/150‑1 (MQ=255)
                                                                                                                        cTACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatga                             >  2:3275173/1‑150 (MQ=255)
                                                                                                                         tACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgat                            <  2:1694659/150‑1 (MQ=255)
                                                                                                                         tACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgat                            >  2:374915/1‑150 (MQ=255)
                                                                                                                         tACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgat                            >  1:2190857/1‑150 (MQ=255)
                                                                                                                         tACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgat                            <  2:1844161/150‑1 (MQ=255)
                                                                                                                         tACACGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgat                            >  2:2387239/1‑150 (MQ=255)
                                                                                                                          acacGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAatgatg                           >  1:2703225/1‑150 (MQ=255)
                                                                                                                           cacGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGt                          >  1:3615473/1‑150 (MQ=255)
                                                                                                                             cGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCTGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcg                        <  2:3900206/150‑1 (MQ=255)
                                                                                                                             cGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcg                        <  2:1534882/150‑1 (MQ=255)
                                                                                                                             cGTTCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcg                        <  1:3239629/150‑1 (MQ=255)
                                                                                                                              gTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcgc                       >  1:1783818/1‑150 (MQ=255)
                                                                                                                               ttCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcgcg                      <  1:2955554/150‑1 (MQ=255)
                                                                                                                               gtCAGCGGTCAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTcgcg                      <  2:3832188/149‑1 (MQ=255)
                                                                                                                                  aGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGAt                   >  2:1413087/1‑150 (MQ=255)
                                                                                                                                    cGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATgg                 <  1:3004130/150‑1 (MQ=255)
                                                                                                                                     ggTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGt                <  1:853479/150‑1 (MQ=255)
                                                                                                                                       tCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGc              <  1:1856832/150‑1 (MQ=255)
                                                                                                                                        cAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGcc             <  1:4111106/150‑1 (MQ=255)
                                                                                                                                        cAGTTCCGGCTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGcc             <  1:2576698/150‑1 (MQ=255)
                                                                                                                                          gTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCa           <  1:2168121/150‑1 (MQ=255)
                                                                                                                                                 cTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTc    <  2:2520905/150‑1 (MQ=255)
                                                                                                                                                 cTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTc    >  1:4002275/1‑150 (MQ=255)
                                                                                                                                                 cTGAAGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTc    <  2:1822875/150‑1 (MQ=255)
                                                                                                                                                   gACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGc  <  1:4322275/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTTCTACACGTTCAGCGGTCAGTTCCGGCTGACGGTCTTCGTCGATAGCCAGACCGACAAAGTGGTCGTCATCTGCCAGACCTTTTGATGCTTCGAAATGATAGCCCGCAGTTGGCCAGTGACCAACGATGGTTGCACCGCGCGGTTCAATGATGTCGCGGATGGTGCCCAATGCGTCGC  >  CP009273/706330‑706626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑