BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	734,498	C→A	7.7%	I14I (ATC→ATA)	ybgA →	DUF1722 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	734,498	0	C	A	7.7%	932.8 / 34.0	252	I14I (ATC→ATA)	ybgA	DUF1722 family protein
Reads supporting (aligned to +/- strand): ref base C (127/105); new base A (15/5); total (142/110)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCA  >  CP009273/734349‑734647

ttGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATc                                                                                                                                                       >  2:3594390/1‑150 (MQ=255)

 tGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCt                                                                                                                                                      >  1:4409135/1‑150 (MQ=255)

 tGCGGGGAGCAGGCTAACGTAAATATGTCCTGCAACAACAGGATTAAATTCAAAAATATCATATCTAAACGTCTACACTTACTCCTGTAAAACGAACAGGATCATTAATAAATCTAAAACGATTAGATGACAGCACACTAATCCGTATCt                                                                                                                                                      >  2:2620734/1‑150 (MQ=255)

    ggggAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTg                                                                                                                                                   >  1:2572942/1‑150 (MQ=255)

    ggggAGCAGGCTGTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTg                                                                                                                                                   <  2:3606408/150‑1 (MQ=255)

      ggAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTgac                                                                                                                                                 <  2:4096477/150‑3 (MQ=255)

          cAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTc                                                                                                                                             <  2:1326386/150‑1 (MQ=255)

            ggCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCAt                                                                                                                                           <  2:626214/150‑1 (MQ=255)

            ggCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCAt                                                                                                                                           <  2:55200/150‑1 (MQ=255)

              cTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGa                                                                                                                                         >  1:338848/1‑150 (MQ=255)

                 tCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGtt                                                                                                                                      >  1:1132780/1‑150 (MQ=255)

                 tCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGtt                                                                                                                                      >  2:1986561/1‑150 (MQ=255)

                    tAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACa                                                                                                                                   >  1:3882370/1‑150 (MQ=255)

                    tAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACa                                                                                                                                   >  1:3485128/1‑150 (MQ=255)

                       aTTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCg                                                                                                                                >  2:3570366/1‑150 (MQ=255)

                       aTTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCg                                                                                                                                <  1:3783372/150‑1 (MQ=255)

                            tCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGa                                                                                                                           >  2:4511892/1‑150 (MQ=255)

                            tCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGa                                                                                                                           >  1:3595578/1‑150 (MQ=255)

                             ccTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGaa                                                                                                                          >  2:1548409/1‑150 (MQ=255)

                               tGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAg                                                                                                                        >  2:2873127/1‑150 (MQ=255)

                               tGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAg                                                                                                                        >  1:3892171/1‑150 (MQ=255)

                                gCTAAAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGa                                                                                                                       <  1:755651/150‑1 (MQ=255)

                                   acaacaGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGACAGAACa                                                                                                                    >  1:2907099/1‑150 (MQ=255)

                                    caacaGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACAt                                                                                                                   >  2:889279/1‑150 (MQ=255)

                                     aacaGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATg                                                                                                                  >  1:928989/1‑150 (MQ=255)

                                        aGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCt                                                                                                               <  1:2618604/150‑1 (MQ=255)

                                        aGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCt                                                                                                               <  2:709955/150‑1 (MQ=255)

                                          gATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTa                                                                                                             >  1:2305359/1‑150 (MQ=255)

                                            ttAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGAGTAGATGACAGCACCCTAATCCGTATATTTGCCATTCATGAGTTACATCGACTGAAAGAACATGGCTTAAc                                                                                                           >  1:1807355/1‑150 (MQ=255)

                                              aaCTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAAcgc                                                                                                         >  2:2966416/1‑150 (MQ=255)

                                                 ttCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAAcgcgcg                                                                                                      <  1:3593564/150‑1 (MQ=255)

                                                   cacaAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCggg                                                                                                    >  1:1445035/1‑150 (MQ=255)

                                                   cacaAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCggg                                                                                                    <  1:1590089/150‑1 (MQ=255)

                                                      aaaTATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgcg                                                                                                 >  2:2243569/1‑150 (MQ=255)

                                                      aaaTATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgcg                                                                                                 <  1:3935632/150‑1 (MQ=255)

                                                      aaaTATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgcg                                                                                                 <  1:1677780/150‑1 (MQ=255)

                                                        atatCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCt                                                                                               <  1:4218076/150‑1 (MQ=255)

                                                         tatCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCtt                                                                                              >  1:2794424/1‑150 (MQ=255)

                                                         tatCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCtt                                                                                              <  1:2838233/150‑1 (MQ=255)

                                                         tatCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCtt                                                                                              >  2:1453495/1‑150 (MQ=255)

                                                          atCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtc                                                                                             >  2:3785886/1‑150 (MQ=255)

                                                          atCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtc                                                                                             >  2:3786653/1‑150 (MQ=255)

                                                            cATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtctc                                                                                           <  1:3970000/150‑1 (MQ=255)

                                                            cATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtctc                                                                                           >  2:879544/1‑150 (MQ=255)

                                                               ttCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGAt                                                                                        >  1:4328765/1‑150 (MQ=255)

                                                                tctcAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGAtt                                                                                       <  1:3145493/150‑1 (MQ=255)

                                                                tctcAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGAtt                                                                                       <  1:2352965/150‑1 (MQ=255)

                                                                tctcAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGAtt                                                                                       <  2:3328540/150‑1 (MQ=255)

                                                                tatcAACGTCTACACTTACTCCTTTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACTGCACCGTAATCCGTATCTTTGCCCTTCATGTGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGATTCTCGAtt                                                                                       <  2:4253254/148‑1 (MQ=255)

                                                                   cAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATc                                                                                    >  1:407719/1‑150 (MQ=255)

                                                                   cAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATc                                                                                    <  1:2857191/150‑1 (MQ=255)

                                                                     aCGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATcac                                                                                  <  1:1339562/150‑1 (MQ=255)

                                                                       gTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAg                                                                                <  2:2510089/150‑1 (MQ=255)

                                                                         cTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGcc                                                                              >  2:2060787/1‑150 (MQ=255)

                                                                         cTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGcc                                                                              >  2:1735860/1‑150 (MQ=255)

                                                                           acacTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGc                                                                            >  1:197564/1‑150 (MQ=255)

                                                                             acTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCta                                                                          >  1:2662830/1‑150 (MQ=255)

                                                                              cTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCtat                                                                         <  2:1178969/150‑1 (MQ=255)

                                                                               ttACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCtata                                                                        <  2:3633331/150‑1 (MQ=255)

                                                                                tACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCtatta                                                                       <  1:4529597/150‑3 (MQ=255)

                                                                                 aCTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATaaa                                                                      >  1:3573724/1‑150 (MQ=255)

                                                                                  cTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAAc                                                                     >  1:684755/1‑150 (MQ=255)

                                                                                  cTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAAc                                                                     >  1:4289450/1‑150 (MQ=255)

                                                                                     cTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCg                                                                  >  2:3200272/1‑150 (MQ=255)

                                                                                      tGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGt                                                                 >  2:1526693/1‑150 (MQ=255)

                                                                                             cGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTg                                                          >  1:2706667/1‑150 (MQ=255)

                                                                                              gCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTgg                                                         >  1:4524843/1‑150 (MQ=255)

                                                                                                 cAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGgcgc                                                      >  1:3297311/1‑150 (MQ=255)

                                                                                                   ggAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCAt                                                    >  1:2965385/1‑150 (MQ=255)

                                                                                                     aGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATtc                                                  >  1:1118459/1‑150 (MQ=255)

                                                                                                        aGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTCTAAACTCGTCTTTCTGGCGCATTCTCa                                               >  1:3249613/1‑150 (MQ=255)

                                                                                                        aGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCa                                               <  2:1519247/150‑1 (MQ=255)

                                                                                                        aGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCa                                               <  1:1202253/150‑1 (MQ=255)

                                                                                                         gTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAg                                              <  2:2576258/150‑1 (MQ=255)

                                                                                                           aaTGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGcc                                            <  1:4233982/150‑1 (MQ=255)

                                                                                                            aTGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCg                                           >  1:743493/1‑150 (MQ=255)

                                                                                                                aTCTACACCGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGt                                       <  2:4410858/150‑1 (MQ=255)

                                                                                                                 tCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTa                                      <  2:3359205/150‑1 (MQ=255)

                                                                                                                 tCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTa                                      <  1:2483641/150‑1 (MQ=255)

                                                                                                                    aCAACGATATGATGACAGCACCCTAATCCGAATCTTTGACCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTATCTGGCGCATTCTCAGCCGGAGAACCg                                   >  1:3390172/1‑150 (MQ=255)

                                                                                                                     cAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGc                                  >  1:3111264/1‑150 (MQ=255)

                                                                                                                       aCGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCaa                                <  2:801085/150‑1 (MQ=255)

                                                                                                                         gATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAAc                              >  1:1588831/1‑150 (MQ=255)

                                                                                                                          aTTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACt                             >  1:1356060/1‑150 (MQ=255)

                                                                                                                             tgatgaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTgg                          <  1:538936/150‑1 (MQ=255)

                                                                                                                                tgaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGccc                       <  2:1132780/150‑1 (MQ=255)

                                                                                                                                 gaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCg                      >  1:549916/1‑150 (MQ=255)

                                                                                                                                 gaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCg                      >  1:3760434/1‑150 (MQ=255)

                                                                                                                                     gCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCg                  <  1:1315134/150‑1 (MQ=255)

                                                                                                                                          cTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCt             <  2:2556688/150‑1 (MQ=255)

                                                                                                                                           tAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTg            >  2:112811/1‑150 (MQ=255)

                                                                                                                                            aaTCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGa           >  2:3072603/1‑150 (MQ=255)

                                                                                                                                            aaTCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGa           <  2:2377312/150‑1 (MQ=255)

                                                                                                                                             aTCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGat          >  2:2249343/1‑150 (MQ=255)

                                                                                                                                             aTCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGat          >  2:2892402/1‑150 (MQ=255)

                                                                                                                                               ccGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGatat        >  1:833690/1‑150 (MQ=255)

                                                                                                                                               ccGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGatat        >  1:3115855/1‑150 (MQ=255)

                                                                                                                                               ccGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGatat        <  2:1636412/150‑1 (MQ=255)

                                                                                                                                                     cTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCa  >  1:3470669/1‑150 (MQ=255)

                                                                                                                                                     cTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCa  >  1:3470875/1‑150 (MQ=255)

TTGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCA  >  CP009273/734349‑734647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`