Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 813,435 C→T 10.1% F312F (TTC→TTT)  moaA → molybdopterin biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273813,4350CT10.1% 848.8 / 64.4 266F312F (TTC→TTT) moaAmolybdopterin biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base C (120/119);  new base T (10/17);  total (130/136)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

CTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTCGCGGTGAAGA  >  CP009273/813286‑813584
                                                                                                                                                     |                                                                                                                                                     
cTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCACCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCTGGAGAAGAAACAGACCCATTTc                                                                                                                                                       >  2:1656578/1‑150 (MQ=255)
cTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTc                                                                                                                                                       >  2:2969773/1‑150 (MQ=255)
cTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTc                                                                                                                                                       >  2:1656340/1‑150 (MQ=255)
 tGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTcc                                                                                                                                                      >  1:4347661/1‑150 (MQ=255)
      gTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGTTCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCAt                                                                                                                                                 >  2:1303914/1‑150 (MQ=255)
      gTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCAt                                                                                                                                                 <  2:3739676/150‑1 (MQ=255)
      gTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCAt                                                                                                                                                 >  1:1751144/1‑150 (MQ=255)
      gTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCAt                                                                                                                                                 <  2:1384397/150‑1 (MQ=255)
           ctccATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCaaaa                                                                                                                                            <  1:1274683/150‑1 (MQ=255)
               aTTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAaccaac                                                                                                                                        <  1:3295095/150‑1 (MQ=255)
                 tGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTTCTGCATCAAAACAacac                                                                                                                                      <  1:3338983/150‑1 (MQ=255)
                  ggTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACAcc                                                                                                                                     <  1:2801733/150‑1 (MQ=255)
                  ggTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACAcc                                                                                                                                     <  1:2802347/150‑1 (MQ=255)
                       aCTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTAt                                                                                                                                >  2:1262767/1‑150 (MQ=255)
                          ccATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTTCTGCATCAAAACAACACCGGTATTAc                                                                                                                             <  2:2046669/150‑1 (MQ=255)
                          ccATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTAc                                                                                                                             >  1:2492412/1‑150 (MQ=255)
                          ccATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTAc                                                                                                                             >  2:545150/1‑150 (MQ=255)
                           cATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACg                                                                                                                            <  1:3277563/150‑1 (MQ=255)
                            aTCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGc                                                                                                                           >  1:1446626/1‑150 (MQ=255)
                            aTCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGc                                                                                                                           >  1:2587089/1‑150 (MQ=255)
                            aTCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGc                                                                                                                           <  2:101618/150‑1 (MQ=255)
                               tctGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCaaa                                                                                                                        >  2:4484449/1‑150 (MQ=255)
                                 tGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAAc                                                                                                                      <  1:3726715/150‑1 (MQ=255)
                                     tGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTAt                                                                                                                  >  1:2294595/1‑150 (MQ=255)
                                     tGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTAt                                                                                                                  >  1:4391080/1‑150 (MQ=255)
                                      gTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATc                                                                                                                 <  2:341931/150‑1 (MQ=255)
                                       tttGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATca                                                                                                                <  1:266804/150‑2 (MQ=255)
                                          ggTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTAc                                                                                                             >  2:2249599/1‑150 (MQ=255)
                                          ggTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTAc                                                                                                             >  2:2779235/1‑150 (MQ=255)
                                           gTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACa                                                                                                            <  2:1815772/150‑1 (MQ=255)
                                           gTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACa                                                                                                            >  1:1280425/1‑150 (MQ=255)
                                             gAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACAtt                                                                                                          <  1:3899961/150‑1 (MQ=255)
                                             gAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACAtt                                                                                                          <  2:283508/150‑1 (MQ=255)
                                               aGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTgg                                                                                                        >  1:4479287/1‑150 (MQ=255)
                                               aGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTgg                                                                                                        <  2:3235977/150‑1 (MQ=255)
                                                ggcggcGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTggc                                                                                                       >  1:7189/1‑150 (MQ=255)
                                                 gcggcgTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTggcg                                                                                                      <  1:1054413/150‑1 (MQ=255)
                                                 gcggcgTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTggcg                                                                                                      <  2:3877086/150‑1 (MQ=255)
                                                   ggcgTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTggcggc                                                                                                    >  2:624383/1‑150 (MQ=255)
                                                        tAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTaaaa                                                                                               <  2:216966/150‑1 (MQ=255)
                                                         aaCCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCAGTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAAc                                                                                              >  2:4463517/1‑150 (MQ=255)
                                                                    tCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgag                                                                                   <  2:1719568/150‑1 (MQ=255)
                                                                     ctgctgGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTTCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgaga                                                                                  >  1:2539929/1‑150 (MQ=255)
                                                                      tgctgGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgagag                                                                                 >  1:1121521/1‑150 (MQ=255)
                                                                       gctgGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGgagaga                                                                                >  2:1861913/1‑150 (MQ=255)
                                                                         tgGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTCTTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATc                                                                              >  2:2321001/1‑150 (MQ=255)
                                                                         tgGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTGATCGTACCTTGGCGGCTAAAACGTCACAAGGAGAGATc                                                                              >  2:4391779/1‑150 (MQ=255)
                                                                          ggAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCa                                                                             <  2:241796/150‑1 (MQ=255)
                                                                           gAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAg                                                                            <  1:3151036/150‑1 (MQ=255)
                                                                            aaGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGa                                                                           >  1:3664108/1‑150 (MQ=255)
                                                                             aGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGAt                                                                          <  1:3040423/150‑1 (MQ=255)
                                                                             aGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGAt                                                                          >  2:4103866/1‑150 (MQ=255)
                                                                              gACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATg                                                                         >  2:1125984/1‑150 (MQ=255)
                                                                              gACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATg                                                                         <  1:2390180/150‑1 (MQ=255)
                                                                               aCGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGa                                                                        <  2:1772619/150‑1 (MQ=255)
                                                                                       cAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTa                                                                >  1:1572888/1‑150 (MQ=255)
                                                                                           aaCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCa                                                            >  1:3885652/1‑150 (MQ=255)
                                                                                           aaCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCa                                                            >  1:137869/1‑150 (MQ=255)
                                                                                            aCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCAc                                                           >  1:3300819/1‑150 (MQ=255)
                                                                                            aCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCAc                                                           >  1:2337857/1‑150 (MQ=255)
                                                                                            aCAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCAc                                                           >  1:4255088/1‑150 (MQ=255)
                                                                                                        aGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTTCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATccc                                               <  2:237593/150‑1 (MQ=255)
                                                                                                        aGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATccc                                               >  1:1969201/1‑150 (MQ=255)
                                                                                                        aGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATccc                                               >  1:1969150/1‑150 (MQ=255)
                                                                                                            cgTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATAAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGAcc                                           <  2:3014042/150‑1 (MQ=255)
                                                                                                              tATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCg                                         >  1:980105/1‑150 (MQ=255)
                                                                                                              tATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCg                                         <  1:4067451/150‑1 (MQ=255)
                                                                                                                 ttCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTAt                                      <  1:3951206/150‑1 (MQ=255)
                                                                                                                  tCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTAtt                                     <  1:808364/150‑1 (MQ=255)
                                                                                                                       ggcgCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTAt                                >  1:4313784/1‑150 (MQ=255)
                                                                                                                       ggcgCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTAt                                >  2:3870697/1‑150 (MQ=255)
                                                                                                                        gcgcTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTAtt                               <  2:2937424/150‑1 (MQ=255)
                                                                                                                         cgcTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTc                              <  2:2667373/150‑1 (MQ=255)
                                                                                                                              cGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACg                         <  2:4303454/150‑1 (MQ=255)
                                                                                                                               gggAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACgg                        >  1:1285373/1‑150 (MQ=255)
                                                                                                                               gggAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGATAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACgg                        <  1:2768343/150‑1 (MQ=255)
                                                                                                                                 gagaAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGtt                      >  1:578743/1‑150 (MQ=255)
                                                                                                                                   gaagaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTc                    >  2:4543729/1‑150 (MQ=255)
                                                                                                                                    aagaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACCTTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCt                   <  2:3813764/150‑1 (MQ=255)
                                                                                                                                    aagaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCt                   >  2:1580148/1‑150 (MQ=255)
                                                                                                                                    aagaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCt                   >  2:711580/1‑150 (MQ=255)
                                                                                                                                    aagaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCt                   <  1:3566418/150‑1 (MQ=255)
                                                                                                                                    aagaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAAATTATCCCGACCCGTATTGCTATTCTTACGGTTTCt                   >  2:3840427/1‑150 (MQ=255)
                                                                                                                                     agaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTa                  >  2:4518533/1‑150 (MQ=255)
                                                                                                                                     agaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTATTACGGTTTCTa                  >  2:2734594/1‑150 (MQ=255)
                                                                                                                                     agaaACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAAATTAACGGACATTGGCGGCGAAAACTTCACACGGAGAGATCAGATGAGTCAGGTAAGCACTGAATATATACCGACCCGTATAGCTATTCTAACGGATTCTa                  >  2:803961/1‑150 (MQ=255)
                                                                                                                                        aaCAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAAtc               <  1:3377571/150‑1 (MQ=255)
                                                                                                                                          cAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAAtcgt             >  2:213025/1‑150 (MQ=255)
                                                                                                                                           aGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAAtcgtc            >  1:3504393/1‑150 (MQ=255)
                                                                                                                                           aGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAAtcgtc            >  1:3000048/1‑150 (MQ=255)
                                                                                                                                           aGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAAtcgtc            <  2:4151046/150‑1 (MQ=255)
                                                                                                                                            gACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAAtcgtcg           >  1:443657/1‑150 (MQ=255)
                                                                                                                                              cccATTTCCTGCATCACAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTcgcg         <  2:3545159/150‑1 (MQ=255)
                                                                                                                                              cccATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTcgcg         >  1:4245181/1‑150 (MQ=255)
                                                                                                                                              cccATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTcgcg         <  2:1280425/150‑1 (MQ=255)
                                                                                                                                              cccATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTcgcg         >  1:4245008/1‑150 (MQ=255)
                                                                                                                                                cATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTCGCGGt       <  1:1849319/150‑1 (MQ=255)
                                                                                                                                                  tttCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTCGCGGTga     <  2:3356549/150‑1 (MQ=255)
                                                                                                                                                  tttCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATAGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTCGCGGTga     <  1:1617912/150‑1 (MQ=255)
                                                                                                                                                   ttCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTCGCGGTgaa    >  1:2122035/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGGAGAGATCAGATGAGTCAGGTAAGCACTGAATTTATCCCGACCCGTATTGCTATTCTTACGGTTTCTAATCGTCGCGGTGAAGA  >  CP009273/813286‑813584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑