Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 943,573 G→T 92.0% A297S (GCC→TCC)  ycaM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273943,5730GT92.0% 841.3 / 62.2 286A297S (GCC→TCC) ycaMputative transporter
Reads supporting (aligned to +/- strand):  ref base G (12/11);  new base T (142/121);  total (154/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 286 total aligned reads displayed.

GTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAT  >  CP009273/943426‑943719
                                                                                                                                                   |                                                                                                                                                  
   tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCAtt                                                                                                                                               <  2:3996111/150‑1 (MQ=255)
   tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCAtt                                                                                                                                               <  1:2592454/150‑1 (MQ=255)
   tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCAtt                                                                                                                                               <  2:1100385/150‑1 (MQ=255)
   tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCAtt                                                                                                                                               <  1:1776410/150‑1 (MQ=255)
   tgtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCAtt                                                                                                                                               <  2:1397353/150‑1 (MQ=255)
    gtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATTTCTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTg                                                                                                                                              <  1:2928742/150‑1 (MQ=255)
    gtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTg                                                                                                                                              >  2:3612399/1‑150 (MQ=255)
        cATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGaa                                                                                                                                          >  2:2099953/1‑150 (MQ=255)
         aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAAt                                                                                                                                         >  2:4361176/1‑150 (MQ=255)
         aTTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAAt                                                                                                                                         <  2:615564/150‑1 (MQ=255)
          ttCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATa                                                                                                                                        <  2:1660132/150‑1 (MQ=255)
           tCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATAc                                                                                                                                       <  2:3399041/150‑1 (MQ=255)
              gggCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATGTACTCCATTGCGAATACCCt                                                                                                                                    >  2:1306738/1‑150 (MQ=255)
               ggCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTg                                                                                                                                   >  1:4020120/1‑150 (MQ=255)
               ggCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTg                                                                                                                                   >  1:1368989/1‑150 (MQ=255)
                    gCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACa                                                                                                                              >  1:3802316/1‑150 (MQ=255)
                      tGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAg                                                                                                                            <  1:3048035/150‑1 (MQ=255)
                       ggCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGt                                                                                                                           <  2:84404/150‑1 (MQ=255)
                        gCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTa                                                                                                                          <  2:586810/150‑1 (MQ=255)
                           aTGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcg                                                                                                                       <  2:494080/150‑1 (MQ=255)
                              gggATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcggcg                                                                                                                    >  2:1503255/1‑150 (MQ=255)
                                gatgatGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCt                                                                                                                  >  2:2969617/1‑150 (MQ=255)
                                 atgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTg                                                                                                                 <  2:1355872/150‑1 (MQ=255)
                                 atgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTg                                                                                                                 <  2:4253016/150‑1 (MQ=255)
                                  tgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTgg                                                                                                                >  1:3989720/1‑150 (MQ=255)
                                   gatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGt                                                                                                               >  2:352483/1‑150 (MQ=255)
                                    atgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTa                                                                                                              >  1:1608530/1‑150 (MQ=255)
                                     tgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAt                                                                                                             >  1:210463/1‑150 (MQ=255)
                                     tgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAt                                                                                                             <  1:4283534/150‑1 (MQ=255)
                                         tGATTCGCGTAATATCCCGGATGACTTAATGACCACCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                         <  1:832017/150‑1 (MQ=255)
                                         tGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                         <  1:197752/150‑1 (MQ=255)
                                         tGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                         <  1:697055/150‑1 (MQ=255)
                                          gATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCg                                                                                                        >  1:1785172/1‑150 (MQ=255)
                                           aTTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                       >  2:4451658/1‑150 (MQ=255)
                                             tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGAtt                                                                                                     <  1:543680/150‑1 (MQ=255)
                                              cgcgTAATATCCCGGATGACTGAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTg                                                                                                    <  2:2868648/150‑1 (MQ=255)
                                               gcgTAATATCCCGGATGACTTACTGACCCACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGa                                                                                                   <  2:2368161/150‑1 (MQ=255)
                                                cgTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTCTTATCGATTGAt                                                                                                  >  2:2729642/1‑150 (MQ=255)
                                                cgTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGAt                                                                                                  >  2:2345153/1‑150 (MQ=255)
                                                 gTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                                 >  2:2646377/1‑150 (MQ=255)
                                                 gTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATg                                                                                                 <  2:2214941/150‑1 (MQ=255)
                                                  tAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGAAGCGGCGCTGGTATTCTCGATTGATGc                                                                                                >  1:3675651/1‑150 (MQ=255)
                                                      atCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCg                                                                                            <  2:2728091/150‑1 (MQ=255)
                                                         ccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCtt                                                                                         <  1:100739/150‑1 (MQ=255)
                                                         ccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCtt                                                                                         <  1:1459419/150‑1 (MQ=255)
                                                            gATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaaa                                                                                      <  2:66362/150‑1 (MQ=255)
                                                             aTGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAg                                                                                     <  2:1424760/150‑1 (MQ=255)
                                                               gACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTg                                                                                   <  2:2428360/150‑1 (MQ=255)
                                                                aCTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGc                                                                                  >  1:22636/1‑150 (MQ=255)
                                                                 cTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCt                                                                                 >  2:1468539/1‑150 (MQ=255)
                                                                    aaTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                              >  1:4396870/1‑150 (MQ=255)
                                                                    aaTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                              >  1:1307131/1‑150 (MQ=255)
                                                                    aaTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTAtt                                                                              >  1:2994341/1‑150 (MQ=255)
                                                                         ccAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTg                                                                         <  2:1141858/150‑1 (MQ=255)
                                                                           aaCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGAt                                                                       <  1:1925512/150‑1 (MQ=255)
                                                                           aaCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGAt                                                                       <  1:3124785/150‑1 (MQ=255)
                                                                             cGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGc                                                                     <  1:3909886/150‑1 (MQ=255)
                                                                              ggTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTTTTAGGTGATGCt                                                                    <  2:4393136/150‑1 (MQ=255)
                                                                              ggTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCt                                                                    <  1:87192/150‑1 (MQ=255)
                                                                                  aGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACa                                                                <  2:2291806/150‑1 (MQ=255)
                                                                                  aGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACa                                                                >  1:3148969/1‑150 (MQ=255)
                                                                                  aGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACa                                                                >  1:3149032/1‑150 (MQ=255)
                                                                                   gTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAg                                                               >  2:3063043/1‑150 (MQ=255)
                                                                                   gTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAg                                                               >  2:1885001/1‑150 (MQ=255)
                                                                                      ttACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCGATTAGGTGATGCTGACAGCaa                                                            >  1:1177577/1‑150 (MQ=255)
                                                                                         cGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAata                                                         >  1:2649656/1‑150 (MQ=255)
                                                                                             tttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATAtt                                                     >  1:1769999/1‑150 (MQ=255)
                                                                                              ttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTc                                                    >  1:4001493/1‑150 (MQ=255)
                                                                                              ttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTc                                                    <  1:2411703/150‑1 (MQ=255)
                                                                                               tCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcc                                                   <  1:1098174/150‑1 (MQ=255)
                                                                                               tCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcc                                                   <  2:3916057/150‑1 (MQ=255)
                                                                                                cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                  >  1:2629084/1‑150 (MQ=255)
                                                                                                cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCACATATATTcca                                                  >  2:3295615/1‑150 (MQ=255)
                                                                                                 aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                                 >  1:4521918/1‑150 (MQ=255)
                                                                                                 aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                                 <  1:2880416/150‑1 (MQ=255)
                                                                                                    aGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagcca                                              >  2:2072208/1‑150 (MQ=255)
                                                                                                            gAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACACTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGACAGTTTATGt                                      >  2:4315474/1‑150 (MQ=255)
                                                                                                             aGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTc                                     <  1:1414983/150‑1 (MQ=255)
                                                                                                               tATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGt                                   >  2:1984696/1‑150 (MQ=255)
                                                                                                               tATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCAGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGt                                   >  1:3697337/1‑150 (MQ=255)
                                                                                                                   acaacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                               >  1:4058171/1‑150 (MQ=255)
                                                                                                                   acaacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                               <  1:1313503/150‑1 (MQ=255)
                                                                                                                   acaacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                               <  1:722855/150‑1 (MQ=255)
                                                                                                                    caacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCaa                              >  2:337410/1‑150 (MQ=255)
                                                                                                                      acaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACg                            <  1:2044886/150‑1 (MQ=255)
                                                                                                                      acaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACg                            >  1:3518290/1‑150 (MQ=255)
                                                                                                                        aTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCt                          >  2:4326679/1‑150 (MQ=255)
                                                                                                                          gggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTc                        >  2:4090244/1‑150 (MQ=255)
                                                                                                                          gggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTc                        >  2:4195287/1‑150 (MQ=255)
                                                                                                                          gggTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTc                        >  2:1562026/1‑150 (MQ=255)
                                                                                                                              aaTACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGt                    >  2:2886566/1‑150 (MQ=255)
                                                                                                                               aTACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTa                   >  2:2486090/1‑150 (MQ=255)
                                                                                                                                   tttAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGcc               >  2:3815011/1‑150 (MQ=255)
                                                                                                                                     tAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCg             >  1:3620934/1‑150 (MQ=255)
                                                                                                                                     tAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCg             >  2:4490544/1‑150 (MQ=255)
                                                                                                                                       aTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGtt           <  1:972294/150‑1 (MQ=255)
                                                                                                                                       aTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGtt           >  1:1137095/1‑150 (MQ=255)
                                                                                                                                        tGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa          >  2:267267/1‑150 (MQ=255)
                                                                                                                                        tGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa          >  1:1536104/1‑150 (MQ=255)
                                                                                                                                          gTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAAt        <  2:1478181/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAT  >  CP009273/943426‑943719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑