Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 958,417 A→T 7.7% N323Y (AAC→TAC)  rpsA → 30S ribosomal subunit protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273958,4170AT 7.7% 929.2 / 50.7 286N323Y (AAC→TAC) rpsA30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base A (112/152);  new base T (13/9);  total (125/161)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 288 total aligned reads displayed.

GTTATCCGGAAGGTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAG  >  CP009273/958268‑958566
                                                                                                                                                     |                                                                                                                                                     
gTTATCCGGAAGGTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTa                                                                                                                                                       <  1:3930764/150‑1 (MQ=255)
  tATCCGGAAGGTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAAc                                                                                                                                                     <  2:2237608/150‑1 (MQ=255)
   aTCCGGAAGGTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACg                                                                                                                                                    >  1:4072767/1‑150 (MQ=255)
    tCCGGAAGGTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGt                                                                                                                                                   >  2:3385998/1‑150 (MQ=255)
           ggTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGAt                                                                                                                                            >  1:1836911/1‑150 (MQ=255)
            gTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATg                                                                                                                                           <  2:2415796/150‑1 (MQ=255)
             tACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGt                                                                                                                                          <  2:235096/150‑1 (MQ=255)
              aCCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTa                                                                                                                                         <  1:3809476/150‑1 (MQ=255)
                 aagctgactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTg                                                                                                                                      >  2:3955823/4‑150 (MQ=255)
                 aaaCTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTg                                                                                                                                      >  2:1699011/1‑150 (MQ=255)
                 aaaCTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTg                                                                                                                                      <  1:2702258/150‑1 (MQ=255)
                 aaaCTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTg                                                                                                                                      <  1:3954713/150‑1 (MQ=255)
                  aaCTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTgg                                                                                                                                     >  1:2202707/1‑150 (MQ=255)
                  aaCTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTgg                                                                                                                                     >  2:667411/1‑150 (MQ=255)
                   actgactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa                                                                                                                                    <  2:3761806/150‑1 (MQ=255)
                   actgactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGa                                                                                                                                    <  2:4187363/150‑1 (MQ=255)
                    ctgactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTGGaa                                                                                                                                   >  2:3130858/1‑150 (MQ=255)
                    ctgactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGaa                                                                                                                                   <  2:1195434/150‑1 (MQ=255)
                    ctgactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGaa                                                                                                                                   <  1:3218545/150‑1 (MQ=255)
                      gactgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGt                                                                                                                                 <  2:1445348/150‑1 (MQ=255)
                        ctgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTGGAAGTTa                                                                                                                               >  1:524482/1‑150 (MQ=255)
                        ctgGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTa                                                                                                                               >  1:2497602/1‑150 (MQ=255)
                          ggTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTGCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATg                                                                                                                             <  1:4442313/150‑1 (MQ=255)
                             cgcgTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGtt                                                                                                                          <  1:377448/150‑1 (MQ=255)
                                 tGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTgg                                                                                                                      >  2:1360772/1‑150 (MQ=255)
                                   aCCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGat                                                                                                                    <  1:576894/150‑1 (MQ=255)
                                     cAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGatat                                                                                                                  >  2:2272284/1‑150 (MQ=255)
                                      aaCCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATc                                                                                                                 >  2:4329697/1‑150 (MQ=255)
                                          tGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATcgacg                                                                                                             >  1:4260226/1‑150 (MQ=255)
                                           gACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATcgacga                                                                                                            >  1:4133856/1‑150 (MQ=255)
                                             ccGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACgaag                                                                                                          <  2:2757393/150‑1 (MQ=255)
                                             ccGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACgaag                                                                                                          <  2:1244761/150‑1 (MQ=255)
                                               gACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACgaagaa                                                                                                        <  1:2801479/150‑1 (MQ=255)
                                                  tACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgt                                                                                                     <  2:3781868/150‑1 (MQ=255)
                                                   aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTTACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtc                                                                                                    <  2:3239326/150‑1 (MQ=255)
                                                   aCGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtc                                                                                                    <  1:1279723/150‑1 (MQ=255)
                                                    cGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcg                                                                                                   >  1:3044815/1‑150 (MQ=255)
                                                     ggCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgt                                                                                                  >  2:1812164/1‑150 (MQ=255)
                                                     ggCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgt                                                                                                  >  2:2576570/1‑150 (MQ=255)
                                                      gctgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgtc                                                                                                 >  2:4320225/1‑150 (MQ=255)
                                                      gctgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgtc                                                                                                 <  2:573256/150‑1 (MQ=255)
                                                       ctgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATTGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgtcg                                                                                                >  1:2928430/1‑150 (MQ=255)
                                                        tgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgtcgt                                                                                               >  1:1455416/1‑150 (MQ=255)
                                                        tgctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgtcgt                                                                                               >  1:2873606/1‑150 (MQ=255)
                                                        tgctTAGTTGAAATCGAAGAAGTCGTTGAAGTCCTGGTACACGTATCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGATAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAAcgtcgtcgt                                                                                               <  2:2648497/150‑1 (MQ=255)
                                                         gctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTa                                                                                              <  1:1479119/150‑1 (MQ=255)
                                                         gctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGGTGTTTACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTa                                                                                              >  1:1934101/1‑150 (MQ=255)
                                                          ctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTAt                                                                                             <  1:3094281/150‑1 (MQ=255)
                                                          ctTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGGTAACGTTGGCGATGTCGTGGAAGTTATGGTTCTGGCTATCGACGAAGACCGTCGTCGTAt                                                                                             >  2:4362390/1‑150 (MQ=255)
                                                              gTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTcc                                                                                         >  2:2412812/1‑150 (MQ=255)
                                                              gTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTcc                                                                                         <  2:4330186/150‑1 (MQ=255)
                                                              gTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTcc                                                                                         <  2:2084549/150‑1 (MQ=255)
                                                                   aaTCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTggg                                                                                    <  1:2517713/150‑1 (MQ=255)
                                                                        aagtagGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACCTCCACCAGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTATGGATATCGACGAAGAACGTCGTCGTATCTCTCTGGGTCTGa                                                                               <  2:3667706/150‑1 (MQ=255)
                                                                        aagaagGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGa                                                                               >  1:2603716/1‑150 (MQ=255)
                                                                        aagaagGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGa                                                                               >  2:4497055/1‑150 (MQ=255)
                                                                           aagGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAAc                                                                            <  2:3460932/150‑1 (MQ=255)
                                                                                  tGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCaa                                                                     >  1:812543/1‑150 (MQ=255)
                                                                                    aaGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAg                                                                   >  2:3403069/1‑150 (MQ=255)
                                                                                      ggCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCt                                                                 >  2:2164987/1‑150 (MQ=255)
                                                                                        ccTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTaa                                                               <  1:3876055/150‑1 (MQ=255)
                                                                                         cTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAAc                                                              >  1:1043751/1‑150 (MQ=255)
                                                                                         cTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAAc                                                              >  2:3091190/1‑150 (MQ=255)
                                                                                          tGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGTTATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAAcc                                                             >  2:4416108/1‑150 (MQ=255)
                                                                                           ggTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAAccc                                                            <  1:4320225/150‑1 (MQ=255)
                                                                                            gTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCg                                                           <  1:2238563/150‑1 (MQ=255)
                                                                                             tACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGt                                                          >  1:2541064/1‑150 (MQ=255)
                                                                                                acGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGgc                                                       >  1:1061725/1‑150 (MQ=255)
                                                                                                 cGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGgca                                                      >  1:2900453/1‑150 (MQ=255)
                                                                                                  gTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGgcag                                                     <  1:1537786/150‑1 (MQ=255)
                                                                                                     tCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGcagcag                                                  <  2:4106888/150‑1 (MQ=255)
                                                                                                         aaaTGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTcg                                              >  2:1114542/1‑150 (MQ=255)
                                                                                                         aaaTGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTcg                                              <  2:2434138/150‑1 (MQ=255)
                                                                                                            tggactggacCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCgg                                           <  2:2733696/150‑1 (MQ=255)
                                                                                                            tggactggacCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCgg                                           >  1:4103142/1‑150 (MQ=255)
                                                                                                               actggacCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGGAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGACTGAAACAGAGCAAAGCTAACCCGTGGCAGCAGTTCGCGGaaa                                        >  1:3252608/1‑150 (MQ=255)
                                                                                                                       aacaaAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCacaac                                >  1:3722738/1‑150 (MQ=255)
                                                                                                                        acaaAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCacaaca                               >  1:2277028/1‑150 (MQ=255)
                                                                                                                         caaAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCAcaacaa                              <  1:2840408/150‑1 (MQ=255)
                                                                                                                          aaaaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAg                             >  1:3998189/1‑150 (MQ=255)
                                                                                                                            aaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAggg                           >  1:3179733/1‑150 (MQ=255)
                                                                                                                            aaaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAggg                           >  1:901330/1‑150 (MQ=255)
                                                                                                                             aaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGACACCCACAACAAGGGc                          >  2:281147/1‑150 (MQ=255)
                                                                                                                             aaCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGc                          >  2:4193076/1‑150 (MQ=255)
                                                                                                                              aCATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCg                         <  1:2511940/150‑1 (MQ=255)
                                                                                                                               cATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGa                        <  2:1874566/150‑1 (MQ=255)
                                                                                                                                aTCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACCACCAGGGCGAc                       >  2:2970647/1‑150 (MQ=255)
                                                                                                                                  ccACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCg                     <  1:3386206/150‑1 (MQ=255)
                                                                                                                                      ccGTCCAAAGTTGTTTACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGtt                 <  1:745923/150‑1 (MQ=255)
                                                                                                                                      ccGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGtt                 >  1:3343670/1‑150 (MQ=255)
                                                                                                                                       cGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTg                >  1:2671656/1‑150 (MQ=255)
                                                                                                                                          ccAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAg             <  2:829623/150‑1 (MQ=255)
                                                                                                                                              aGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTaa         <  2:1470948/150‑1 (MQ=255)
                                                                                                                                               gttgttAACGTTGGCGATGTAGTGGAATTTATGGTTCTTGATATCGACGAAGAACGTAGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTaaa        <  2:3839653/150‑1 (MQ=255)
                                                                                                                                               gttgttAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTaaa        <  2:316958/150‑1 (MQ=255)
                                                                                                                                                  gttAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATc     <  1:4510195/150‑1 (MQ=255)
                                                                                                                                                   ttAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCa    <  2:1286661/150‑1 (MQ=255)
                                                                                                                                                    tAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCaa   <  2:3279742/150‑1 (MQ=255)
                                                                                                                                                    tAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCGCCCTGGGTCTGAAACAGTGCAACGCTAACCCGGGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCaa   <  2:2503101/150‑1 (MQ=255)
                                                                                                                                                     aaCGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAg  >  1:274122/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTATCCGGAAGGTACCAAACTGACTGGTCGCGTGACCAACCTGACCGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGCCTGGTACACGTTTCCGAAATGGACTGGACCAACAAAAACATCCACCCGTCCAAAGTTGTTAACGTTGGCGATGTAGTGGAAGTTATGGTTCTGGATATCGACGAAGAACGTCGTCGTATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAG  >  CP009273/958268‑958566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑