BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,060,203	G→A	9.7%	V238I (GTT→ATT)	yccE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,060,203	0	G	A	9.7%	779.2 / 56.9	246	V238I (GTT→ATT)	yccE	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (127/95); new base A (11/13); total (138/108)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 246 total aligned reads displayed.

ATCTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTAT  >  CP009273/1060055‑1060351

atCTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGt                                                                                                                                                     >  2:2144280/1‑150 (MQ=255)

 tCTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGtt                                                                                                                                                    <  2:1697581/150‑1 (MQ=255)

  cTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGttt                                                                                                                                                   <  2:3781297/150‑1 (MQ=255)

  cTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAAttt                                                                                                                                                   <  2:2771994/150‑1 (MQ=255)

  cTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAAttt                                                                                                                                                   <  2:2772333/150‑1 (MQ=255)

    tttATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTAc                                                                                                                                                 >  1:3951039/1‑150 (MQ=255)

        ttCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACg                                                                                                                                             <  1:756315/150‑1 (MQ=255)

         tCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGa                                                                                                                                            >  1:1502128/1‑150 (MQ=255)

         tCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGa                                                                                                                                            >  1:711400/1‑150 (MQ=255)

          cAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGaa                                                                                                                                           <  2:1776881/150‑1 (MQ=255)

           aGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAAt                                                                                                                                          >  2:2456681/1‑150 (MQ=255)

           aGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAAt                                                                                                                                          <  2:1668103/150‑1 (MQ=255)

            gCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAAtt                                                                                                                                         <  2:301737/150‑1 (MQ=255)

            gCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAAtt                                                                                                                                         <  1:561178/150‑1 (MQ=255)

            gCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAAtt                                                                                                                                         <  2:474153/150‑1 (MQ=255)

            gCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAAtt                                                                                                                                         <  2:474584/150‑1 (MQ=255)

              gTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCa                                                                                                                                       >  2:3979586/1‑150 (MQ=255)

              gTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCa                                                                                                                                       <  1:3315170/150‑1 (MQ=255)

               tGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCAt                                                                                                                                      >  1:1625521/1‑150 (MQ=255)

                gAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCAtt                                                                                                                                     <  1:1916136/150‑1 (MQ=255)

                gAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCAtt                                                                                                                                     >  2:2219855/1‑150 (MQ=255)

                     tAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAAt                                                                                                                                >  2:2822842/1‑150 (MQ=255)

                     tAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTACTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTATAAt                                                                                                                                >  2:2822775/1‑150 (MQ=255)

                      aaCCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATg                                                                                                                               <  2:4367769/150‑1 (MQ=255)

                       aCCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATgg                                                                                                                              <  2:1054144/150‑1 (MQ=255)

                         cAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGtt                                                                                                                            >  2:1843498/1‑150 (MQ=255)

                          agtcATTATGATTAAAATATGCTCCTGTAGATGGCGATGTATATTGTCTGTTTAGATCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTg                                                                                                                           <  2:933503/147‑1 (MQ=255)

                          aGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTTTTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTg                                                                                                                           >  2:3454402/1‑150 (MQ=255)

                          aGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTg                                                                                                                           <  2:1174240/150‑1 (MQ=255)

                          aGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTg                                                                                                                           <  2:1174129/150‑1 (MQ=255)

                          aGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTg                                                                                                                           <  1:3455365/150‑1 (MQ=255)

                          aGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTg                                                                                                                           <  2:933250/150‑1 (MQ=255)

                           ggCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGa                                                                                                                          >  1:2004750/1‑150 (MQ=255)

                           ggCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGa                                                                                                                          <  1:3856243/150‑1 (MQ=255)

                            gCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGAt                                                                                                                         >  1:3659362/1‑150 (MQ=255)

                              aTTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATaa                                                                                                                       <  2:2008720/150‑1 (MQ=255)

                                 aGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATaaaaa                                                                                                                    <  2:2921275/150‑1 (MQ=255)

                                  ggATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATaaaaaa                                                                                                                   >  1:3580119/1‑150 (MQ=255)

                                  ggATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATaaaaaa                                                                                                                   >  1:3408799/1‑150 (MQ=255)

                                    aTTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAAc                                                                                                                 >  2:1219067/1‑150 (MQ=255)

                                      tAAAATATGCTCCTGTAGATGTCGATGGATATTGTCTGTTAAGAGCTATACTGTTTTTAAAACAACATGATTATTCATGGGCGCTTGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGa                                                                                                               <  2:4045986/150‑1 (MQ=255)

                                       aaaaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGAt                                                                                                              >  1:3651900/1‑150 (MQ=255)

                                       aaaaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGAt                                                                                                              >  1:569157/1‑150 (MQ=255)

                                        aaaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATc                                                                                                             >  1:2839387/1‑150 (MQ=255)

                                        aaaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATc                                                                                                             >  2:4289509/1‑150 (MQ=255)

                                         aaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCg                                                                                                            >  1:1189780/1‑150 (MQ=255)

                                         aaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCg                                                                                                            >  1:326319/1‑150 (MQ=255)

                                         aaTATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCg                                                                                                            >  1:735448/1‑150 (MQ=255)

                                          atatGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGa                                                                                                           <  1:2546828/150‑1 (MQ=255)

                                             tGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGc                                                                                                        >  2:3548743/1‑150 (MQ=255)

                                             tGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGc                                                                                                        >  2:1859954/1‑150 (MQ=255)

                                             tGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGc                                                                                                        >  2:3548780/1‑150 (MQ=255)

                                                tCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGctct                                                                                                     >  2:3573911/1‑150 (MQ=255)

                                                 ccTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCtt                                                                                                    <  1:125443/150‑1 (MQ=255)

                                                       gATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGAt                                                                                              >  1:4031236/1‑150 (MQ=255)

                                                         tGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATAc                                                                                            <  2:3951039/150‑1 (MQ=255)

                                                            cGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGc                                                                                         >  1:3176619/1‑150 (MQ=255)

                                                                 gATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCt                                                                                    >  2:2945246/1‑150 (MQ=255)

                                                                   tatTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCtat                                                                                  >  1:3957816/1‑150 (MQ=255)

                                                                        tCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAAtct                                                                             >  1:2831536/1‑150 (MQ=255)

                                                                              aaGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGa                                                                       >  1:2712450/1‑150 (MQ=255)

                                                                                gagCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGTTACGGCATTCTATAATCTCAGGGAAg                                                                     >  1:4403683/1‑150 (MQ=255)

                                                                                 agCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGa                                                                    >  1:2276239/1‑150 (MQ=255)

                                                                                  gCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGAt                                                                   <  2:2207655/150‑1 (MQ=255)

                                                                                   cTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATg                                                                  >  2:34046/1‑150 (MQ=255)

                                                                                   cTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATg                                                                  <  1:297189/150‑1 (MQ=255)

                                                                                     ataCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTa                                                                >  2:2281409/1‑150 (MQ=255)

                                                                                       aCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTaaa                                                              >  2:1906379/1‑150 (MQ=255)

                                                                                       aCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGAT‑AAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAg                                                             >  2:1420703/1‑150 (MQ=255)

                                                                                        cTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGCATGATCAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAg                                                             <  2:3027692/150‑1 (MQ=255)

                                                                                               tAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTAt                                                      >  2:1117514/1‑150 (MQ=255)

                                                                                                aaaaCAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTAtt                                                     <  2:266608/150‑1 (MQ=255)

                                                                                                     aacaTGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCg                                                >  1:3779057/1‑150 (MQ=255)

                                                                                                     aacaTGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCg                                                >  2:337469/1‑150 (MQ=255)

                                                                                                      acaTGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGt                                               >  1:798058/1‑150 (MQ=255)

                                                                                                         tGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGa                                            >  2:3285911/1‑150 (MQ=255)

                                                                                                          gATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGAt                                           >  1:3430925/1‑150 (MQ=255)

                                                                                                          gATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGAt                                           <  1:4127345/150‑1 (MQ=255)

                                                                                                             tattCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTa                                        <  2:2768620/150‑1 (MQ=255)

                                                                                                               ttCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACa                                      <  2:2484411/150‑1 (MQ=255)

                                                                                                               gtCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACa                                      <  2:182923/149‑1 (MQ=255)

                                                                                                                 cATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAAt                                    >  2:408617/1‑150 (MQ=255)

                                                                                                                   tGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCt                                  <  2:569157/150‑1 (MQ=255)

                                                                                                                     ggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGa                                <  1:2807124/150‑1 (MQ=255)

                                                                                                                      gcgcTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGAc                               <  1:2456681/150‑1 (MQ=255)

                                                                                                                         cTGGTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAAc                            >  2:2467290/1‑150 (MQ=255)

                                                                                                                           ggTCAGTTATAAGATGCAAAAGGAAATTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACATCCa                          <  2:3359686/150‑1 (MQ=255)

                                                                                                                               aGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAAtt                      >  2:1740119/1‑150 (MQ=255)

                                                                                                                               aGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAAtt                      >  2:1740093/1‑150 (MQ=255)

                                                                                                                                 ttATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCa                    <  2:3469937/150‑1 (MQ=255)

                                                                                                                                    taAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAggg                 <  1:2281409/150‑1 (MQ=255)

                                                                                                                                          gCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCagag           <  1:650102/150‑1 (MQ=255)

                                                                                                                                          gCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCagag           >  2:3416482/1‑150 (MQ=255)

                                                                                                                                           cAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagt          >  1:85491/1‑150 (MQ=255)

                                                                                                                                           cAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagt          >  1:2152896/1‑150 (MQ=255)

                                                                                                                                           cAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagt          >  1:1125724/1‑150 (MQ=255)

                                                                                                                                            aaaaGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagta         <  2:829869/150‑1 (MQ=255)

                                                                                                                                             aaaGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagtag        >  2:3917402/1‑150 (MQ=255)

                                                                                                                                              aaGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGagtagt       >  2:2253978/1‑150 (MQ=255)

                                                                                                                                                   aGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGCGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTAt  <  1:1843498/150‑1 (MQ=255)

ATCTTTTATTCAGCGTGAAACTAACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTAT  >  CP009273/1060055‑1060351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`