Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,150,407 G→T 44.3% P280P (CCG→CCT)  yceG → septation protein, ampicillin sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,150,4070GT44.3% 226.4 / inf 275P280P (CCG→CCT) yceGseptation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base G (72/81);  new base T (78/44);  total (150/125)
Fisher's exact test for biased strand distribution p-value = 7.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 286 total aligned reads displayed.

GTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGATTA  >  CP009273/1150258‑1150556
                                                                                                                                                     |                                                                                                                                                     
gTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTgccg                                                                                                                                                       <  2:2258648/150‑1 (MQ=255)
    ttATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAg                                                                                                                                                   >  2:2829691/1‑150 (MQ=255)
    ttATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg                                                                                                                                                   <  2:539144/150‑1 (MQ=255)
     tATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTCCGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACTCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAgg                                                                                                                                                  >  2:1326277/1‑150 (MQ=255)
     tATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAgg                                                                                                                                                  <  1:4174034/150‑1 (MQ=255)
        cAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGc                                                                                                                                               >  1:2318277/1‑150 (MQ=255)
          aCCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGa                                                                                                                                             <  1:652922/150‑1 (MQ=255)
           ccGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGAt                                                                                                                                            <  2:3227761/150‑1 (MQ=255)
                          tATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                             <  1:2892312/150‑1 (MQ=255)
                           aTGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCg                                                                                                                            >  2:1655846/1‑150 (MQ=255)
                           aTGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCg                                                                                                                            >  2:268302/1‑150 (MQ=255)
                                ccTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTc                                                                                                                       <  1:1223455/150‑1 (MQ=255)
                                 cTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCg                                                                                                                      >  1:1506125/1‑150 (MQ=255)
                                 cTGCAGACCGACCCGACCGTGATTTAAGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCg                                                                                                                      >  2:4400201/1‑150 (MQ=255)
                                    cAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTg                                                                                                                   >  1:2872036/1‑150 (MQ=255)
                                     aGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGa                                                                                                                  >  2:565934/1‑150 (MQ=255)
                                     aGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGa                                                                                                                  >  2:3087981/1‑150 (MQ=255)
                                       accgaccCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAg                                                                                                                <  2:3843707/150‑1 (MQ=255)
                                            cccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgctgc                                                                                                           >  1:627642/1‑150 (MQ=255)
                                            cccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgctgc                                                                                                           >  1:1416909/1‑150 (MQ=255)
                                             ccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgctgct                                                                                                          <  2:2231859/150‑1 (MQ=255)
                                                    tGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATc                                                                                                   >  1:3630402/1‑150 (MQ=255)
                                                     gATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATcc                                                                                                  <  1:1039646/150‑1 (MQ=255)
                                                     gATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATcc                                                                                                  <  2:3365022/150‑1 (MQ=255)
                                                         tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCa                                                                                              >  1:1977284/1‑150 (MQ=255)
                                                         tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCa                                                                                              >  1:692826/1‑150 (MQ=255)
                                                         tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCa                                                                                              >  1:3059471/1‑150 (MQ=255)
                                                         tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCa                                                                                              >  2:3126722/1‑150 (MQ=255)
                                                         tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCa                                                                                              >  1:1150886/1‑150 (MQ=255)
                                                            gggATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCaaaa                                                                                           >  2:1555438/1‑150 (MQ=255)
                                                            gggATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCaaaa                                                                                           >  2:1555184/1‑150 (MQ=255)
                                                               aTGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACg                                                                                        <  1:1488016/150‑1 (MQ=255)
                                                               aTGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACg                                                                                        <  2:730791/150‑1 (MQ=255)
                                                                    agagCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTa                                                                                   >  1:854205/1‑150 (MQ=255)
                                                                      agCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTAtc                                                                                 >  1:395267/1‑150 (MQ=255)
                                                                         gTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATctct                                                                              <  2:3280946/150‑1 (MQ=255)
                                                                           tataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAt                                                                            >  2:3698527/1‑150 (MQ=255)
                                                                            ataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAtt                                                                           >  1:791136/1‑150 (MQ=255)
                                                                             taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAttt                                                                          <  1:337377/150‑1 (MQ=255)
                                                                              aaTGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAtttt                                                                         >  2:3580115/1‑150 (MQ=255)
                                                                               aTGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTtg                                                                        <  1:3277792/150‑1 (MQ=255)
                                                                                 ggCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTtgtg                                                                      >  1:396255/1‑150 (MQ=255)
                                                                                 ggCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTtgtg                                                                      <  1:2841067/150‑1 (MQ=255)
                                                                                    aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGcc                                                                   >  1:4234886/1‑150 (MQ=255)
                                                                                     aaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCg                                                                  >  2:1773211/1‑150 (MQ=255)
                                                                                        tttCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATg                                                               >  1:4356870/1‑150 (MQ=255)
                                                                                          tctcGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGt                                                             <  1:1043611/150‑1 (MQ=255)
                                                                                          tctcGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGt                                                             <  1:1834872/150‑1 (MQ=255)
                                                                                             cGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTcaa                                                          >  2:4503113/1‑150 (MQ=255)
                                                                                              gTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAg                                                         >  2:207080/1‑150 (MQ=255)
                                                                                                gCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAggt                                                       <  2:1846355/150‑1 (MQ=255)
                                                                                                 cAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATGACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAggtg                                                      <  2:3702115/150‑1 (MQ=255)
                                                                                                      cTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTcac                                                 >  1:483206/1‑150 (MQ=255)
                                                                                                       tGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTcaca                                                <  1:1270374/150‑1 (MQ=255)
                                                                                                       tGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTcaca                                                <  1:2279306/150‑1 (MQ=255)
                                                                                                       tGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTcaca                                                <  2:1163592/150‑1 (MQ=255)
                                                                                                       tGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTcaca                                                <  2:1164072/150‑1 (MQ=255)
                                                                                                         gAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACg                                              <  2:1938644/150‑1 (MQ=255)
                                                                                                           aaCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGtt                                            <  2:692826/150‑1 (MQ=255)
                                                                                                           aaCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGtt                                            <  2:3052883/150‑1 (MQ=255)
                                                                                                            aCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTACAGGTGGTCACACGttt                                           >  1:4409168/1‑150 (MQ=255)
                                                                                                            aCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGttt                                           <  2:869589/150‑1 (MQ=255)
                                                                                                            aCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGttt                                           >  1:3484147/1‑150 (MQ=255)
                                                                                                             cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTa                                          >  2:4172495/1‑150 (MQ=255)
                                                                                                             cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTa                                          <  1:1631340/150‑1 (MQ=255)
                                                                                                             cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTa                                          >  1:2545293/1‑150 (MQ=255)
                                                                                                              gCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTaa                                         <  1:50468/150‑1 (MQ=255)
                                                                                                                cGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATa                                       >  2:1809291/1‑150 (MQ=255)
                                                                                                                cGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATa                                       >  2:1807476/1‑150 (MQ=255)
                                                                                                                cGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATa                                       <  2:2330267/150‑1 (MQ=255)
                                                                                                                 gACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATAc                                      <  1:2388271/150‑1 (MQ=255)
                                                                                                                 gACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATAc                                      <  1:2127908/150‑1 (MQ=255)
                                                                                                                  aCAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATAcc                                     <  1:1268088/150‑1 (MQ=255)
                                                                                                                  aCAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATAcc                                     <  2:937891/150‑1 (MQ=255)
                                                                                                                   cAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCa                                    >  1:4144740/1‑150 (MQ=255)
                                                                                                                    aGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCaa                                   >  2:946106/1‑150 (MQ=255)
                                                                                                                      cGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATc                                 <  2:3205805/150‑1 (MQ=255)
                                                                                                                      cGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATc                                 <  1:3532310/150‑1 (MQ=255)
                                                                                                                      cGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATc                                 <  1:3532381/150‑1 (MQ=255)
                                                                                                                          taACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGc                             >  1:3114321/1‑150 (MQ=255)
                                                                                                                          taACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGc                             <  2:2534548/150‑1 (MQ=255)
                                                                                                                             cacCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAg                          >  1:4002970/1‑150 (MQ=255)
                                                                                                                               ccTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTc                        >  2:1098305/1‑150 (MQ=255)
                                                                                                                                cTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCa                       <  2:1514643/150‑1 (MQ=255)
                                                                                                                                  ataCCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATa                     >  1:1483199/1‑150 (MQ=255)
                                                                                                                                   taCCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGGTTAATACCAATCTTGCCAGTCATaa                    >  2:2127815/1‑150 (MQ=255)
                                                                                                                                     ccATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACa                  <  1:268302/150‑1 (MQ=255)
                                                                                                                                       aTTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAg                <  2:2318223/150‑1 (MQ=255)
                                                                                                                                       aTTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAg                >  2:1366296/1‑150 (MQ=255)
                                                                                                                                       aTTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGGGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAg                >  2:963978/1‑150 (MQ=255)
                                                                                                                                         tACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTc              >  1:2919420/1‑150 (MQ=255)
                                                                                                                                          aCCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCt             <  1:1775516/150‑1 (MQ=255)
                                                                                                                                          aCCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCt             <  1:2979988/150‑1 (MQ=255)
                                                                                                                                               tCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCa        <  1:1381327/150‑1 (MQ=255)
                                                                                                                                                 tGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAgg      <  1:2253232/150‑1 (MQ=255)
                                                                                                                                                 tGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAgg      <  2:3967496/150‑1 (MQ=255)
                                                                                                                                                  gcctccAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGa     >  1:3851586/1‑150 (MQ=255)
                                                                                                                                                    cgccAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGAtt   <  1:2960836/150‑1 (MQ=255)
                                                                                                                                                    cgccAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGAtt   <  1:2738999/150‑1 (MQ=255)
                                                                                                                                                     gccAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGATTa  >  1:1536033/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGATTA  >  CP009273/1150258‑1150556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑