Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,251,120 T→A 15.0% T97S (ACC→TCC)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,251,1200TA15.0% 620.6 / 88.3 220T97S (ACC→TCC) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base T (104/83);  new base A (21/12);  total (125/95)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 222 total aligned reads displayed.

CATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATC  >  CP009273/1250973‑1251267
                                                                                                                                                   |                                                                                                                                                   
cATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGgaga                                                                                                                                                   <  2:1291637/150‑1 (MQ=255)
cATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGa                                                                                                                                                   >  2:3506926/1‑150 (MQ=255)
  tGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAca                                                                                                                                                 >  1:2226035/1‑150 (MQ=255)
  tGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAca                                                                                                                                                 >  1:2096987/1‑150 (MQ=255)
      tataGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAcatcat                                                                                                                                             <  2:2581303/150‑1 (MQ=255)
       ataGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCAtt                                                                                                                                            <  2:2735291/150‑1 (MQ=255)
       ataGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCAtt                                                                                                                                            <  2:2735235/150‑1 (MQ=255)
        taGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTa                                                                                                                                           >  1:4201081/1‑150 (MQ=255)
          gTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGACCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTAcc                                                                                                                                         <  2:3681070/150‑1 (MQ=255)
           tCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTAccc                                                                                                                                        >  1:1486771/1‑150 (MQ=255)
           tCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTAccc                                                                                                                                        >  1:1485015/1‑150 (MQ=255)
             aCCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAg                                                                                                                                      <  1:325850/150‑1 (MQ=255)
              ccAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAgg                                                                                                                                     >  1:887330/1‑150 (MQ=255)
              ccAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAgg                                                                                                                                     >  1:887293/1‑150 (MQ=255)
                aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGtt                                                                                                                                   <  1:538397/150‑1 (MQ=255)
                aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGtt                                                                                                                                   >  2:2637503/1‑150 (MQ=255)
                 aTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTg                                                                                                                                  >  2:4222965/1‑150 (MQ=255)
                  ttAAGTTAATGCCGATGGCAGATGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGTGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGa                                                                                                                                 <  2:3517508/150‑1 (MQ=255)
                    aaGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGata                                                                                                                               <  1:423041/150‑1 (MQ=255)
                      gTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATAtt                                                                                                                             <  1:149698/150‑1 (MQ=255)
                      gTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATAtt                                                                                                                             <  1:268984/150‑1 (MQ=255)
                         aaTGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGcc                                                                                                                          >  1:1326768/1‑150 (MQ=255)
                         aaTGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGcc                                                                                                                          >  1:4498421/1‑150 (MQ=255)
                          aTGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCa                                                                                                                         >  2:547689/1‑150 (MQ=255)
                                 tGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTTACATCATTACCCAGGTTGATATTGCCAGGCgtgt                                                                                                                  >  2:4409749/1‑150 (MQ=255)
                                  ggCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGtt                                                                                                                 >  1:4273435/1‑150 (MQ=255)
                                  ggCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGtt                                                                                                                 <  2:1853577/150‑1 (MQ=255)
                                   gCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGttt                                                                                                                <  1:1422386/150‑1 (MQ=255)
                                     agagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTcc                                                                                                              <  2:253940/150‑1 (MQ=255)
                                      gagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCct                                                                                                             >  1:3929242/1‑150 (MQ=255)
                                       agGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctg                                                                                                            <  2:310646/150‑1 (MQ=255)
                                       agGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctg                                                                                                            <  2:2132338/150‑1 (MQ=255)
                                        ggTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgc                                                                                                           >  2:283399/1‑150 (MQ=255)
                                        ggTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgc                                                                                                           <  1:3441508/150‑1 (MQ=255)
                                        ggTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgc                                                                                                           >  1:2498564/1‑150 (MQ=255)
                                         gTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgct                                                                                                          >  1:2883708/1‑150 (MQ=255)
                                            tGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTggg                                                                                                       >  1:3311518/1‑150 (MQ=255)
                                                  acaacaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCAtt                                                                                                 <  1:1678063/150‑1 (MQ=255)
                                                  acaacaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCAtt                                                                                                 >  1:657715/1‑150 (MQ=255)
                                                     acaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACACAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTaca                                                                                              <  1:253006/150‑1 (MQ=255)
                                                       aTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACAcc                                                                                            <  2:3177171/150‑1 (MQ=255)
                                                         cTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCAt                                                                                          >  1:4104355/1‑150 (MQ=255)
                                                          tACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATa                                                                                         <  1:3760349/150‑1 (MQ=255)
                                                          tACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATa                                                                                         >  2:1037116/1‑150 (MQ=255)
                                                          tACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATa                                                                                         >  2:4335141/1‑150 (MQ=255)
                                                             tgtgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATagag                                                                                      >  2:2286468/1‑150 (MQ=255)
                                                             tgtgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATagag                                                                                      >  2:3168683/1‑150 (MQ=255)
                                                             tgtgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATagag                                                                                      >  2:3168612/1‑150 (MQ=255)
                                                                gAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGtt                                                                                   <  2:2283268/150‑1 (MQ=255)
                                                                          gCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGa                                                                         <  1:1010863/150‑1 (MQ=255)
                                                                           cTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGAt                                                                        <  2:4278351/150‑1 (MQ=255)
                                                                           cTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGAt                                                                        >  1:1310918/1‑150 (MQ=255)
                                                                           cTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGAt                                                                        <  2:2029600/150‑1 (MQ=255)
                                                                            tGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATc                                                                       <  1:1400420/150‑1 (MQ=255)
                                                                            tGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATc                                                                       >  1:3299359/1‑150 (MQ=255)
                                                                             gTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcg                                                                      >  1:2314962/1‑150 (MQ=255)
                                                                                aGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggcg                                                                   <  2:57340/150‑1 (MQ=255)
                                                                                 gggAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggcgg                                                                  >  1:485878/1‑150 (MQ=255)
                                                                                         tttttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCt                                                          >  1:2782285/1‑150 (MQ=255)
                                                                                           tttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGt                                                        <  2:3590004/150‑1 (MQ=255)
                                                                                              gCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAAt                                                     >  2:923721/1‑150 (MQ=255)
                                                                                                ccTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTc                                                   >  1:851273/1‑150 (MQ=255)
                                                                                                ccTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTc                                                   >  1:1367617/1‑150 (MQ=255)
                                                                                                 cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                  >  1:600297/1‑150 (MQ=255)
                                                                                                 cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                  <  2:2763322/150‑1 (MQ=255)
                                                                                                 cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                  >  2:2342490/1‑150 (MQ=255)
                                                                                                       aatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCAcc                                            >  1:4149759/1‑150 (MQ=255)
                                                                                                        atGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACca                                           >  2:3320907/1‑150 (MQ=255)
                                                                                                        atGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACca                                           >  2:3320364/1‑150 (MQ=255)
                                                                                                         tGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcat                                          >  1:2945170/1‑150 (MQ=255)
                                                                                                          gATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatc                                         >  1:3627383/1‑150 (MQ=255)
                                                                                                           aTTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatca                                        <  2:2172918/150‑1 (MQ=255)
                                                                                                           aTTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatca                                        <  1:1100289/150‑1 (MQ=255)
                                                                                                           aTTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAAGTCTGTCACcatca                                        <  2:2173922/150‑1 (MQ=255)
                                                                                                           aTTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatca                                        <  2:1105901/150‑1 (MQ=255)
                                                                                                            ttCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcatcat                                       >  2:3465858/1‑150 (MQ=255)
                                                                                                             tCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCAtt                                      >  2:2372032/1‑150 (MQ=255)
                                                                                                              ccTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTa                                     >  2:3895619/1‑150 (MQ=255)
                                                                                                               cTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTaa                                    <  2:2872594/150‑1 (MQ=255)
                                                                                                                   tGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGt                                >  2:4133418/1‑150 (MQ=255)
                                                                                                                        atCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAatat                           <  1:3218229/150‑1 (MQ=255)
                                                                                                                         tCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATAtt                          <  1:2567872/150‑1 (MQ=255)
                                                                                                                           cAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTgg                        >  2:740634/1‑150 (MQ=255)
                                                                                                                            agagGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGt                       >  1:2546844/1‑150 (MQ=255)
                                                                                                                            agagGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGt                       <  1:953909/150‑1 (MQ=255)
                                                                                                                               ggCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGcc                    <  1:3390684/150‑1 (MQ=255)
                                                                                                                                 cgtcgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCat                  <  2:2951343/150‑1 (MQ=255)
                                                                                                                                   tcgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCatat                <  1:3067658/150‑1 (MQ=255)
                                                                                                                                    cgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATAtt               <  2:3820958/150‑1 (MQ=255)
                                                                                                                                     gtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTg              <  2:1375743/150‑1 (MQ=255)
                                                                                                                                       tGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGAc            <  1:4092849/150‑1 (MQ=255)
                                                                                                                                        gACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACt           >  1:1906013/1‑150 (MQ=255)
                                                                                                                                        gACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACt           >  1:3289150/1‑150 (MQ=255)
                                                                                                                                          cATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTc         <  2:213049/150‑1 (MQ=255)
                                                                                                                                           aTTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTcc        <  1:399172/150‑1 (MQ=255)
                                                                                                                                           aTTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTcc        >  2:1059222/1‑150 (MQ=255)
                                                                                                                                            ttGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCt       <  2:2810730/150‑1 (MQ=255)
                                                                                                                                             tGACGTTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTTATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTg      <  2:1728041/150‑1 (MQ=255)
                                                                                                                                              gACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGt     <  2:1588352/150‑1 (MQ=255)
                                                                                                                                              gACGGTGACATCATTACCCAGGTTGATAGTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGt     <  2:1586412/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATC  >  CP009273/1250973‑1251267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑