BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,341,061	C→A	48.5%	Y3* (TAC→TAA)	ymiB →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,341,061	0	C	A	48.5%	118.5 / inf	258	Y3* (TAC→TAA)	ymiB	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (53/80); new base A (57/68); total (110/148)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 258 total aligned reads displayed.

ACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATC  >  CP009273/1340914‑1341208

aCATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAAt                                                                                                                                                   <  1:352189/150‑1 (MQ=255)

  aTCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGa                                                                                                                                                 >  1:1020120/1‑150 (MQ=255)

       tgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTa                                                                                                                                            <  2:4092899/150‑1 (MQ=255)

        gctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTaa                                                                                                                                           >  1:4499998/1‑150 (MQ=255)

          tttCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAAcg                                                                                                                                         >  2:548235/1‑150 (MQ=255)

              cGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAAcggcgg                                                                                                                                     <  1:4067382/150‑1 (MQ=255)

               gTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACggcggc                                                                                                                                    >  1:2852941/1‑150 (MQ=255)

               gTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACggcggc                                                                                                                                    >  1:565839/1‑150 (MQ=255)

                tCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCt                                                                                                                                   <  1:762392/150‑1 (MQ=255)

                    ccGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAAc                                                                                                                               >  1:2601233/1‑150 (MQ=255)

                     cGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACg                                                                                                                              <  2:1024845/150‑1 (MQ=255)

                       ccAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCa                                                                                                                            <  2:2019105/150‑1 (MQ=255)

                         aGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGACCAACATGACTTAAATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                          <  2:668257/150‑1 (MQ=255)

                         aGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                          <  2:4192810/150‑1 (MQ=255)

                         aGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                          <  2:4194311/150‑1 (MQ=255)

                         aGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                          <  2:2295425/150‑1 (MQ=255)

                         aGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCAtt                                                                                                                          >  1:4188345/1‑150 (MQ=255)

                         aGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATAAACGGCGGCTAAACGCAtt                                                                                                                          >  1:4188322/1‑150 (MQ=255)

                           tATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGt                                                                                                                        >  1:3736530/1‑150 (MQ=255)

                              ggATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGtttt                                                                                                                     <  2:928904/150‑1 (MQ=255)

                                    tgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTtat                                                                                                               <  1:1050071/150‑1 (MQ=255)

                                     gCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTtata                                                                                                              <  1:575506/150‑1 (MQ=255)

                                       gATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTtatata                                                                                                            >  2:3999853/1‑150 (MQ=255)

                                        aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTatatat                                                                                                           <  2:4539840/150‑1 (MQ=255)

                                         tCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtatt                                                                                                          <  2:3163279/150‑1 (MQ=255)

                                          ccGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattt                                                                                                         <  2:275479/150‑1 (MQ=255)

                                          ccGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattt                                                                                                         <  2:1683443/150‑1 (MQ=255)

                                           cGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattta                                                                                                        >  2:1138262/1‑150 (MQ=255)

                                           cGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattta                                                                                                        >  1:2947016/1‑150 (MQ=255)

                                            gagaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtatttat                                                                                                       <  2:4337327/150‑1 (MQ=255)

                                             agaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtatttatt                                                                                                      <  2:4228864/150‑1 (MQ=255)

                                              gaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATatttattt                                                                                                     >  1:3545798/1‑150 (MQ=255)

                                               aaaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTg                                                                                                    <  1:3387639/150‑1 (MQ=255)

                                                aaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCCTTGTTTTTTTTATATATTTATTTGt                                                                                                   >  1:3553583/1‑150 (MQ=255)

                                                aaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGt                                                                                                   >  1:3755523/1‑150 (MQ=255)

                                                aaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGt                                                                                                   <  1:2773934/150‑1 (MQ=255)

                                                   ttGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAAt                                                                                                <  1:3203450/150‑1 (MQ=255)

                                                   ttGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAAt                                                                                                <  2:3446600/150‑1 (MQ=255)

                                                     gTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATcc                                                                                              <  1:3603425/150‑1 (MQ=255)

                                                       aCAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAg                                                                                            <  2:1624942/150‑1 (MQ=255)

                                                          aCGTTGTGGACTCCCTAACGGTTTTCATGATGCATCTCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAACCGCATTGTTTTTTTTATATATTTATTTGTAATCCAGttt                                                                                         <  1:3299688/150‑1 (MQ=255)

                                                            gTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTg                                                                                       >  1:2481909/1‑150 (MQ=255)

                                                              tgtgGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGa                                                                                     <  1:4465403/150‑1 (MQ=255)

                                                                    cTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACg                                                                               >  1:4365109/1‑150 (MQ=255)

                                                                        cTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg                                                                           <  1:4228669/150‑1 (MQ=255)

                                                                        cTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg                                                                           <  1:564051/150‑1 (MQ=255)

                                                                         tAACTGTTTTCATGATGAATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAAAAACATTACTTAAATTAAATTAACGGCGGCTAAACGCTTTGTTTTTTTTATATATTTATTTGTAATCCTGTTTTGGAAAAACGCCAGt                                                                          <  1:238254/150‑1 (MQ=255)

                                                                         tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGt                                                                          <  2:3033874/150‑1 (MQ=255)

                                                                         tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGt                                                                          <  1:4077052/150‑1 (MQ=255)

                                                                          aaCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtt                                                                         >  1:4312646/1‑150 (MQ=255)

                                                                          aaCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtt                                                                         <  2:1272638/150‑1 (MQ=255)

                                                                           aCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGttt                                                                        <  1:1935811/150‑1 (MQ=255)

                                                                            cGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtttt                                                                       <  2:2383993/150‑1 (MQ=255)

                                                                               ttttCATGATGCATATCCTCCGGTTAACATCTGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTTAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaa                                                                    <  2:4261446/150‑1 (MQ=255)

                                                                               ttttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaa                                                                    >  2:3744527/1‑150 (MQ=255)

                                                                                   cATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACAAGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGa                                                                >  2:1857434/1‑150 (MQ=255)

                                                                                       atgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGt                                                            <  1:132775/150‑1 (MQ=255)

                                                                                       atgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGt                                                            <  2:3942936/150‑1 (MQ=255)

                                                                                          cATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAACAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg                                                         <  1:22660/150‑1 (MQ=255)

                                                                                          cATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg                                                         <  1:1113196/150‑1 (MQ=255)

                                                                                          cATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg                                                         <  1:23357/150‑1 (MQ=255)

                                                                                            tatCCTCCGGTTAACAGCGGAGTCCGCTGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCGGtt                                                       <  2:2866288/150‑1 (MQ=255)

                                                                                             atCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa                                                      <  1:3108302/150‑1 (MQ=255)

                                                                                              tcctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaa                                                     <  1:3360594/150‑1 (MQ=255)

                                                                                                     gTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCt                                              >  1:257945/1‑150 (MQ=255)

                                                                                                       tAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGc                                            >  2:216845/1‑150 (MQ=255)

                                                                                                       tAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGc                                            >  2:2526775/1‑150 (MQ=255)

                                                                                                        aaCAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGcc                                           >  2:3584288/1‑150 (MQ=255)

                                                                                                           aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTgg                                        <  1:3029058/150‑1 (MQ=255)

                                                                                                           aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTgg                                        >  2:2824528/1‑150 (MQ=255)

                                                                                                           aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTgg                                        >  2:4442519/1‑150 (MQ=255)

                                                                                                           aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGACAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTgg                                        >  2:3071044/1‑150 (MQ=255)

                                                                                                                aGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGAtata                                   >  2:614489/1‑150 (MQ=255)

                                                                                                                aGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGAtata                                   >  1:3965234/1‑150 (MQ=255)

                                                                                                                aGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGAtata                                   <  2:3625447/150‑1 (MQ=255)

                                                                                                                   ccGCGGTTAAATAAAAGTAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACg                                <  2:373386/150‑1 (MQ=255)

                                                                                                                   ccGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACg                                >  2:1814658/1‑150 (MQ=255)

                                                                                                                      cgGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAg                             >  2:1886139/1‑150 (MQ=255)

                                                                                                                         ttAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTa                          >  2:551146/1‑150 (MQ=255)

                                                                                                                          taaataaaAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTag                         >  1:3769392/1‑150 (MQ=255)

                                                                                                                          taaataaaAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTag                         >  1:3770767/1‑150 (MQ=255)

                                                                                                                          taaataaaAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTag                         >  1:4536421/1‑150 (MQ=255)

                                                                                                                           aaataaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTaga                        <  2:3755523/150‑1 (MQ=255)

                                                                                                                            aataaaAGGAACAACATGACTTACATGAAATTAACGGCGACTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTagag                       >  2:1636536/1‑150 (MQ=255)

                                                                                                                             ataaaAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGc                      >  1:1362367/1‑150 (MQ=255)

                                                                                                                             ataaaAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGc                      <  2:1369971/150‑1 (MQ=255)

                                                                                                                               aaaaGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCgg                    <  2:1515280/150‑1 (MQ=255)

                                                                                                                                  aGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGa                 <  1:939844/150‑1 (MQ=255)

                                                                                                                                   ggAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGaa                <  1:1770896/150‑1 (MQ=255)

                                                                                                                                   ggAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGaa                <  1:1771139/150‑1 (MQ=255)

                                                                                                                                    gAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGaaa               >  1:3414906/1‑150 (MQ=255)

                                                                                                                                        aacaTGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATaaa           >  1:3087610/1‑150 (MQ=255)

                                                                                                                                         acaTGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAAc          <  1:644886/150‑1 (MQ=255)

                                                                                                                                         acaTGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAAc          <  1:1516628/150‑1 (MQ=255)

                                                                                                                                            tGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGc       >  2:2872552/1‑150 (MQ=255)

                                                                                                                                            tGACTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGc       <  1:2057410/150‑1 (MQ=255)

                                                                                                                                              aCTTACATGAAATTAACGGCGGCTAAACGCTTTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGccc     <  2:2645160/150‑1 (MQ=255)

                                                                                                                                              aCTTAAATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGccc     >  2:2540654/1‑150 (MQ=255)

                                                                                                                                                ttACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCccat   >  1:1071705/1‑150 (MQ=255)

                                                                                                                                                ttACAAGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCccat   >  1:2225214/1‑150 (MQ=255)

ACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATC  >  CP009273/1340914‑1341208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`