BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,343,867	G→A	7.9%	I166I (ATC→ATT)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,343,867	0	G	A	7.9%	721.3 / 36.9	215	I166I (ATC→ATT)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (88/110); new base A (10/7); total (98/117)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 223 total aligned reads displayed.

TAGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGA  >  CP009273/1343718‑1344016

tAGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATg                                                                                                                                                       <  1:56774/150‑1 (MQ=255)

tAGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATg                                                                                                                                                       >  1:2589746/1‑150 (MQ=255)

tAGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATg                                                                                                                                                       <  2:1928824/150‑1 (MQ=255)

tAGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATg                                                                                                                                                       <  2:1928715/150‑1 (MQ=255)

 aGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGa                                                                                                                                                      >  1:1873940/1‑150 (MQ=255)

 aGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGa                                                                                                                                                      >  1:3667088/1‑150 (MQ=255)

  gctgctTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGAt                                                                                                                                                     <  2:3329696/150‑1 (MQ=255)

    tgctTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGAttt                                                                                                                                                   <  1:437209/150‑1 (MQ=255)

     gctTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTg                                                                                                                                                  >  2:1631216/1‑150 (MQ=255)

     gctTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTg                                                                                                                                                  >  2:1631184/1‑150 (MQ=255)

     gctTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTg                                                                                                                                                  <  2:3710750/150‑1 (MQ=255)

        tCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCt                                                                                                                                               <  2:3404666/150‑1 (MQ=255)

         cGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTa                                                                                                                                              >  2:3870902/1‑150 (MQ=255)

            cAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATg                                                                                                                                           <  2:63669/150‑1 (MQ=255)

            cAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATg                                                                                                                                           >  2:1472939/1‑150 (MQ=255)

            cAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATg                                                                                                                                           <  1:1068688/150‑1 (MQ=255)

               cGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATAATTTGGCTAATGATc                                                                                                                                        <  1:3108535/150‑1 (MQ=255)

                gTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATca                                                                                                                                       >  1:2805319/1‑150 (MQ=255)

                        tcatcaGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTcc                                                                                                                               <  2:1169833/150‑1 (MQ=255)

                        tcatcaGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTcc                                                                                                                               >  2:509381/1‑150 (MQ=255)

                         catcaGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAAGCAATGATTTGGCTaa                                                                                                                                             <  1:3686769/135‑1 (MQ=255)

                         catcaGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTaa                                                                                                                                             >  2:3686745/1‑135 (MQ=255)

                         catcaGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTccc                                                                                                                              <  1:2229331/150‑1 (MQ=255)

                           tcaGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAg                                                                                                                            >  2:500261/1‑150 (MQ=255)

                                  aCAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTaa                                                                                                                     <  1:2013241/150‑1 (MQ=255)

                                    aGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTCATGATCACACAGTCCCAGGCAGTAAGa                                                                                                                   >  1:3945829/1‑150 (MQ=255)

                                    aGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGa                                                                                                                   >  1:4279239/1‑150 (MQ=255)

                                    aGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGa                                                                                                                   >  2:1813765/1‑150 (MQ=255)

                                    aGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGa                                                                                                                   >  2:4427000/1‑150 (MQ=255)

                                       gACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCg                                                                                                                <  2:2635508/150‑1 (MQ=255)

                                            gTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTc                                                                                                           <  1:4109565/150‑1 (MQ=255)

                                                gCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATAATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTc                                                                                                       <  1:1112840/150‑1 (MQ=255)

                                                  gTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAg                                                                                                     <  2:1396613/150‑1 (MQ=255)

                                                       tttGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCt                                                                                                <  2:2099729/150‑1 (MQ=255)

                                                           tATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGgcg                                                                                            >  2:1594527/1‑150 (MQ=255)

                                                             tctcCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGcgcg                                                                                          <  2:779148/150‑1 (MQ=255)

                                                              ctcCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGa                                                                                         >  1:1205007/1‑150 (MQ=255)

                                                                ccAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                                                       <  2:4388715/150‑1 (MQ=255)

                                                                 cAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCg                                                                                      <  2:2265112/150‑1 (MQ=255)

                                                                 cAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATAATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCg                                                                                      >  2:708172/1‑150 (MQ=255)

                                                                  aGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCgg                                                                                     >  1:2674755/1‑150 (MQ=255)

                                                                  aGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCgg                                                                                     >  1:2282932/1‑150 (MQ=255)

                                                                    cccGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGtt                                                                                   <  2:1258655/150‑1 (MQ=255)

                                                                     ccGGGTAACCAGCGTACCGGATGCCCGATATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTg                                                                                  <  2:2540431/150‑1 (MQ=255)

                                                                      cGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGt                                                                                 <  2:201153/150‑1 (MQ=255)

                                                                                  gTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                                                     <  1:401837/150‑1 (MQ=255)

                                                                                   tACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAgg                                                                    >  2:668841/1‑150 (MQ=255)

                                                                                     ccGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCg                                                                  >  2:3990669/1‑150 (MQ=255)

                                                                                      cGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGa                                                                 >  2:1043556/1‑150 (MQ=255)

                                                                                       ggATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAg                                                                <  1:1694691/150‑1 (MQ=255)

                                                                                         aTGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCt                                                              <  1:4192968/150‑1 (MQ=255)

                                                                                         aTGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCt                                                              <  1:4193203/150‑1 (MQ=255)

                                                                                            cccGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGc                                                           <  1:942703/150‑1 (MQ=255)

                                                                                             ccGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGcc                                                          <  2:477323/150‑1 (MQ=255)

                                                                                              cGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCa                                                         >  2:1264280/1‑150 (MQ=255)

                                                                                              cGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCa                                                         <  2:3012179/150‑1 (MQ=255)

                                                                                              cGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCa                                                         >  2:3437884/1‑150 (MQ=255)

                                                                                                 gaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATAATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGtt                                                      >  1:3874456/1‑150 (MQ=255)

                                                                                                        cTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGc                                               <  2:1873940/150‑1 (MQ=255)

                                                                                                          gAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCat                                             <  2:2461303/150‑1 (MQ=255)

                                                                                                             aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATAtt                                          <  2:3464346/150‑1 (MQ=255)

                                                                                                             aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATAtt                                          <  2:3464416/150‑1 (MQ=255)

                                                                                                             aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATAtt                                          <  2:221624/150‑1 (MQ=255)

                                                                                                             aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATAtt                                          <  2:221654/150‑1 (MQ=255)

                                                                                                                  tcacttGTCCGCGCCTGAAAGCCAATAACACCTATGATTGGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGTTAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTg                                     <  2:2920039/146‑1 (MQ=255)

                                                                                                                      ttGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAttt                                 <  2:4206168/150‑1 (MQ=255)

                                                                                                                      ttGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAttt                                 <  2:4207536/150‑1 (MQ=255)

                                                                                                                        gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTtgt                               <  1:4080494/150‑1 (MQ=255)

                                                                                                                         tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTtgtg                              >  2:2358435/1‑150 (MQ=255)

                                                                                                                          ccGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGc                             >  2:1618459/1‑150 (MQ=255)

                                                                                                                          ccGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGc                             <  1:4534013/150‑1 (MQ=255)

                                                                                                                           cgcgCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGcc                            <  1:1746877/150‑1 (MQ=255)

                                                                                                                            gcgcCTGAAAGCCAATATAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCa                           >  1:3204064/1‑150 (MQ=255)

                                                                                                                              gcCTGAAAGCCAATAAAACCAATAATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGCCGGGCATATTGCGTGATTTGTGCCAAc                         >  2:201347/1‑150 (MQ=255)

                                                                                                                               ccTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAAcc                        >  1:2852990/1‑150 (MQ=255)

                                                                                                                               ccTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAAcc                        >  1:1359182/1‑150 (MQ=255)

                                                                                                                                 tGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCga                      >  2:3845667/1‑150 (MQ=255)

                                                                                                                                  gAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCgat                     >  2:4529913/1‑150 (MQ=255)

                                                                                                                                      gCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGAtt                 >  1:638471/1‑150 (MQ=255)

                                                                                                                                      gCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTCTGCCAACCGATGAtt                 <  1:4432016/150‑1 (MQ=255)

                                                                                                                                      gCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATAGTGCGTGATTTGTGCCAACCGATGAtt                 <  2:2043688/150‑1 (MQ=255)

                                                                                                                                      gCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGATGGCGCTATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTGGTGCCAACCGATGAtt                 <  1:4432198/150‑1 (MQ=255)

                                                                                                                                       ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTa                <  1:4381287/150‑1 (MQ=255)

                                                                                                                                       ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTa                >  1:940657/1‑150 (MQ=255)

                                                                                                                                        cAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTAc               <  1:3374408/150‑1 (MQ=255)

                                                                                                                                         aaTAAAACCAATAATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACt              >  1:268730/1‑150 (MQ=255)

                                                                                                                                            aaaaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTc           >  1:354679/1‑150 (MQ=255)

                                                                                                                                             aaaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTcc          >  1:3067829/1‑150 (MQ=255)

                                                                                                                                             aaaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTcc          >  1:2077541/1‑150 (MQ=255)

                                                                                                                                             aaaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTcc          >  1:723402/1‑150 (MQ=255)

                                                                                                                                              aaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTccc         <  1:4427000/150‑1 (MQ=255)

                                                                                                                                               aCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCt        <  1:111009/150‑1 (MQ=255)

                                                                                                                                               aCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCt        >  2:1401152/1‑150 (MQ=255)

                                                                                                                                               aCCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCt        >  1:907966/1‑150 (MQ=255)

                                                                                                                                                ccAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCtg       >  1:4188747/1‑150 (MQ=255)

                                                                                                                                                ccAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCtg       <  1:1631187/150‑1 (MQ=255)

                                                                                                                                                ccAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCtg       <  1:1631147/150‑1 (MQ=255)

                                                                                                                                                 cAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCtgg      <  2:4431781/150‑1 (MQ=255)

                                                                                                                                                    tGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCtggtgg   >  2:1187288/1‑150 (MQ=255)

                                                                                                                                                     gATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGa  >  2:4176203/1‑150 (MQ=255)

TAGCTGCTTCGCCAGCGTAATGATTCATCAGCAAACAGTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTGTGCCAACCGATGATTACTTTCCCTGGTGGA  >  CP009273/1343718‑1344016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`