BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,383,189	G→T	10.4%	M1M (ATG→ATT) †	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,383,189	0	G	T	10.4%	982.7 / 60.0	278	M1M (ATG→ATT) †	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base G (137/112); new base T (10/19); total (147/131)
Fisher's exact test for biased strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 283 total aligned reads displayed.

CCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTA  >  CP009273/1383043‑1383338

   tGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAAcc                                                                                                                                                 >  1:1855688/1‑150 (MQ=255)

    ggAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACACCAAGGAGTAAAGATGAGAACCg                                                                                                                                                >  2:3853216/1‑150 (MQ=255)

    ggAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCg                                                                                                                                                <  2:661066/150‑1 (MQ=255)

       aaTGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACACGGAGTAAAGATGAGAACCGTTa                                                                                                                                             >  2:1652457/1‑150 (MQ=255)

       aaTGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTa                                                                                                                                             <  1:3652854/150‑1 (MQ=255)

       aaTGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTa                                                                                                                                             >  2:495518/1‑150 (MQ=255)

        aTGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTaa                                                                                                                                            >  2:1984129/1‑150 (MQ=255)

         tGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAg                                                                                                                                           >  2:2444301/1‑150 (MQ=255)

          gAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAgg                                                                                                                                          <  1:3790187/150‑1 (MQ=255)

          gAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAgg                                                                                                                                          >  2:1221743/1‑150 (MQ=255)

            aCGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTa                                                                                                                                        <  2:2353444/150‑1 (MQ=255)

            aCGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTa                                                                                                                                        <  2:4314315/150‑1 (MQ=255)

             cGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTAt                                                                                                                                       <  1:3159278/150‑1 (MQ=255)

              ggTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTAtt                                                                                                                                      <  1:841937/150‑1 (MQ=255)

                tttGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCg                                                                                                                                    <  1:2467178/150‑1 (MQ=255)

                 ttGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGa                                                                                                                                   >  2:2834957/1‑150 (MQ=255)

                  tgtgACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAg                                                                                                                                  >  2:4389761/1‑150 (MQ=255)

                    tgACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGa                                                                                                                                <  1:3196386/150‑1 (MQ=255)

                    tgACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTCTTCGAGGa                                                                                                                                >  2:4462787/1‑150 (MQ=255)

                     gACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGaa                                                                                                                               >  1:4108928/1‑150 (MQ=255)

                      aCATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGAAg                                                                                                                              <  2:1269671/150‑1 (MQ=255)

                      aCATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAg                                                                                                                              >  2:1975020/1‑150 (MQ=255)

                       cATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGc                                                                                                                             >  2:2375393/1‑150 (MQ=255)

                       cATGATTATCTTTCCTGATTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGc                                                                                                                             <  2:3723090/150‑1 (MQ=255)

                         tGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGGCAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCt                                                                                                                           <  1:2569814/150‑1 (MQ=255)

                          gATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTg                                                                                                                          <  1:2423056/150‑1 (MQ=255)

                          gATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAAGAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGAAGCCTg                                                                                                                          <  2:3683685/150‑1 (MQ=255)

                           aTTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTGGAGGAAGCCTgg                                                                                                                         <  1:170125/150‑1 (MQ=255)

                             tATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGcc                                                                                                                       >  1:296299/1‑150 (MQ=255)

                                cTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCtt                                                                                                                    <  2:2006331/150‑1 (MQ=255)

                                  ttCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTAc                                                                                                                  <  1:2478419/150‑1 (MQ=255)

                                       gTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATAcc                                                                                                             >  1:3715516/1‑150 (MQ=255)

                                         ttACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATAcccc                                                                                                           <  2:1966480/150‑1 (MQ=255)

                                         tgaCATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATAcccc                                                                                                           <  2:1187668/148‑1 (MQ=255)

                                          tACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCg                                                                                                          >  2:4026828/1‑150 (MQ=255)

                                          tACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCg                                                                                                          <  2:3376104/150‑1 (MQ=255)

                                           aCATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGt                                                                                                         >  1:4057967/1‑150 (MQ=255)

                                           aCATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGt                                                                                                         >  1:1805744/1‑150 (MQ=255)

                                            cATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGACGCCTGGCCCTTACATACCCCGtt                                                                                                        >  2:4162384/1‑150 (MQ=255)

                                             atatAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGttt                                                                                                       >  1:3072168/1‑150 (MQ=255)

                                                taGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTtgtg                                                                                                    >  2:2234734/1‑150 (MQ=255)

                                                 aGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGa                                                                                                   <  2:685289/150‑1 (MQ=255)

                                                  gTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTGTGTGAt                                                                                                  >  1:1529620/1‑150 (MQ=255)

                                                    tAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTg                                                                                                <  2:181952/150‑1 (MQ=255)

                                                      aCGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGcc                                                                                              <  1:2849350/150‑1 (MQ=255)

                                                      aCGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAAAGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGcc                                                                                              <  2:4089131/150‑1 (MQ=255)

                                                       cGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGccc                                                                                             <  2:821060/150‑1 (MQ=255)

                                                           acacCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCgggg                                                                                         >  1:3118138/1‑150 (MQ=255)

                                                            cacCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGa                                                                                        <  1:2903323/150‑1 (MQ=255)

                                                              ccTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAg                                                                                      <  1:2012076/150‑1 (MQ=255)

                                                               ctcgTTGATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGACCCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGt                                                                                     <  2:3534559/147‑1 (MQ=255)

                                                               cTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGt                                                                                     >  2:517241/1‑150 (MQ=255)

                                                               cTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGt                                                                                     <  2:3534392/150‑1 (MQ=255)

                                                               cTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTAAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGGTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGt                                                                                     >  2:516753/1‑150 (MQ=255)

                                                                tAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTc                                                                                    >  2:3909991/1‑150 (MQ=255)

                                                                  gTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGc                                                                                  >  1:1469761/1‑150 (MQ=255)

                                                                    ttATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAg                                                                                <  2:4245279/150‑1 (MQ=255)

                                                                         cTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAg                                                                           >  1:775642/1‑150 (MQ=255)

                                                                         cTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAg                                                                           <  2:189201/150‑1 (MQ=255)

                                                                         cTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAg                                                                           >  1:775679/1‑150 (MQ=255)

                                                                         cTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAg                                                                           >  1:767834/1‑150 (MQ=255)

                                                                           aaCTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAgcg                                                                         >  1:2184465/1‑150 (MQ=255)

                                                                           aaCTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAgcg                                                                         <  1:445253/150‑1 (MQ=255)

                                                                            aCTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAgcgc                                                                        <  1:1409489/150‑1 (MQ=255)

                                                                              tGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTCTTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACgcgcgc                                                                      >  2:3568753/1‑150 (MQ=255)

                                                                                 cAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtg                                                                   >  1:334153/1‑150 (MQ=255)

                                                                                      acacAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtggt                                                              <  1:2051961/150‑1 (MQ=255)

                                                                                       cacaGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtggtg                                                             >  1:3446605/1‑150 (MQ=255)

                                                                                       cacaGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtggtg                                                             >  1:3961510/1‑150 (MQ=255)

                                                                                          aGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGtt                                                          >  2:1456695/1‑150 (MQ=255)

                                                                                            cAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGa                                                        >  1:1135021/1‑150 (MQ=255)

                                                                                             aaaCGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGaa                                                       <  1:527392/150‑1 (MQ=255)

                                                                                               aCGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACt                                                     >  2:174393/1‑150 (MQ=255)

                                                                                               aCGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGGGGTGGTGGTTGAACt                                                     >  1:4367649/1‑150 (MQ=255)

                                                                                                 gCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTgg                                                   >  2:2080863/1‑150 (MQ=255)

                                                                                                   tATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGgaa                                                 <  1:4026828/150‑1 (MQ=255)

                                                                                                       tGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGaagaag                                             >  1:2320767/1‑150 (MQ=255)

                                                                                                          gcTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                          >  2:3010344/1‑150 (MQ=255)

                                                                                                          gcTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                          >  2:2795349/1‑150 (MQ=255)

                                                                                                            ttAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGt                                        <  2:3072168/150‑1 (MQ=255)

                                                                                                              aaTCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTAt                                      >  2:408173/1‑150 (MQ=255)

                                                                                                               aTCCGCAGACCACCGCGACAACAAGGAGTAAAGATTAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTAtt                                     <  2:2528770/150‑1 (MQ=255)

                                                                                                                       accaccGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCAc                             <  2:2830253/150‑1 (MQ=255)

                                                                                                                        ccaccGCGACAACTAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCAcc                            <  2:943590/150‑1 (MQ=255)

                                                                                                                        ccaccGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCAcc                            <  2:949214/150‑1 (MQ=255)

                                                                                                                        ccaccGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCAcc                            <  1:1780952/150‑1 (MQ=255)

                                                                                                                           ccGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGc                         <  1:710773/150‑1 (MQ=255)

                                                                                                                             gcgACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGa                       <  2:3469634/150‑1 (MQ=255)

                                                                                                                               gACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAAt                     >  2:1405292/1‑150 (MQ=255)

                                                                                                                               gACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAAt                     >  2:1788953/1‑150 (MQ=255)

                                                                                                                                   acaaGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCAc                 <  1:4285290/150‑1 (MQ=255)

                                                                                                                                   acaaGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCAc                 <  1:2834957/150‑1 (MQ=255)

                                                                                                                                      aGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGcc              <  2:179713/150‑1 (MQ=255)

                                                                                                                                           tAAAGATTAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGGTTGTGATTGCCCGGGGAAGTCGCAGGGAAGCGCGCGTGGTGGTGGTTGAACTGGAGGCTGAGGGTATTAAAGGCACCGGCGACTGGACGCCGTATc         >  2:2711284/1‑150 (MQ=255)

                                                                                                                                            aaaGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATcc        >  1:1677079/1‑150 (MQ=255)

                                                                                                                                              aGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACCTACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCcgc      <  2:929829/150‑1 (MQ=255)

                                                                                                                                              aGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCcgc      >  2:1357499/1‑150 (MQ=255)

                                                                                                                                                 tGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGtt   <  1:935650/150‑1 (MQ=255)

                                                                                                                                                  gagaACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTa  <  2:4057972/150‑1 (MQ=255)

                                                                                                                                                  gagaACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTa  >  1:3623528/1‑150 (MQ=255)

CCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTA  >  CP009273/1383043‑1383338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`