Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,529,506 C→T 5.2% G204S (GGC→AGC)  yddE ← PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,529,5060CT 5.2% 1091.7 / 30.0 305G204S (GGC→AGC) yddEPhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base C (135/154);  new base T (6/10);  total (141/164)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

TTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA  >  CP009273/1529360‑1529654
                                                                                                                                                  |                                                                                                                                                    
tttAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTgg                                                                                                                                                   >  1:76541/1‑150 (MQ=255)
tttAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTgg                                                                                                                                                   >  1:2379336/1‑150 (MQ=255)
    acacGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCgg                                                                                                                                               <  1:2992445/150‑1 (MQ=255)
         cAACACGGTGCCATCGTGGGGCAATACGTTGTGATGTACCACCCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTg                                                                                                                                          <  2:4161794/150‑1 (MQ=255)
             acGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAAc                                                                                                                                      <  2:3031384/150‑1 (MQ=255)
               gTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACgg                                                                                                                                    >  2:3367228/1‑150 (MQ=255)
               gTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACgg                                                                                                                                    >  2:4372109/1‑150 (MQ=255)
                   ccATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaag                                                                                                                                >  1:3358010/1‑150 (MQ=255)
                   ccATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaag                                                                                                                                <  1:3468751/150‑1 (MQ=255)
                   ccATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaag                                                                                                                                <  1:3468981/150‑1 (MQ=255)
                         tGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCa                                                                                                                          >  2:113626/1‑150 (MQ=255)
                         tGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCa                                                                                                                          >  2:1038277/1‑150 (MQ=255)
                          ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                         >  2:3092838/1‑150 (MQ=255)
                           gggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAtt                                                                                                                        <  2:3253208/150‑1 (MQ=255)
                           gggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAtt                                                                                                                        <  2:4334020/150‑1 (MQ=255)
                           gggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAtt                                                                                                                        <  2:3073371/150‑1 (MQ=255)
                           gggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGCAAGAAACCAtt                                                                                                                        >  1:4412132/1‑150 (MQ=255)
                             gCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGc                                                                                                                      <  1:3692120/150‑1 (MQ=255)
                               aaTACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAACCCATTGCAg                                                                                                                    >  2:2780868/1‑150 (MQ=255)
                                  aCGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCa                                                                                                                 >  1:3055964/1‑150 (MQ=255)
                                   cGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCaa                                                                                                                <  1:2882752/150‑1 (MQ=255)
                                     ttGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATc                                                                                                              <  2:4475530/150‑1 (MQ=255)
                                      tgtgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGACCGGGAAGAAACCATTGCAGCCAATCt                                                                                                             >  1:85621/1‑150 (MQ=255)
                                      tgtgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCt                                                                                                             <  2:702139/150‑1 (MQ=255)
                                        tgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttt                                                                                                           <  2:1677331/150‑1 (MQ=255)
                                        tgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttt                                                                                                           <  2:2428476/150‑1 (MQ=255)
                                         gATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCtttt                                                                                                          <  1:2328208/150‑1 (MQ=255)
                                          aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                         <  2:428269/150‑1 (MQ=255)
                                          aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                         >  1:179307/1‑150 (MQ=255)
                                          aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                         <  1:2217488/150‑1 (MQ=255)
                                          aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                         <  2:406869/150‑1 (MQ=255)
                                          aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                         <  2:4002747/150‑1 (MQ=255)
                                           tGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTg                                                                                                        >  1:2201100/1‑150 (MQ=255)
                                           tGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTg                                                                                                        <  1:418073/150‑1 (MQ=255)
                                              accaaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTg                                                                                                     >  1:4213542/1‑150 (MQ=255)
                                              accaaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTg                                                                                                     >  1:3968223/1‑150 (MQ=255)
                                               ccaaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGa                                                                                                    <  1:1099315/150‑1 (MQ=255)
                                               ccaaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGa                                                                                                    <  1:1098382/150‑1 (MQ=255)
                                                caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                   <  1:2102627/150‑1 (MQ=255)
                                                caaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGAt                                                                                                   >  2:3435819/1‑150 (MQ=255)
                                                 aaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATa                                                                                                  >  1:3571729/1‑150 (MQ=255)
                                                 aaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATa                                                                                                  <  1:924108/150‑1 (MQ=255)
                                                    caTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCg                                                                                               >  2:1886814/1‑150 (MQ=255)
                                                     aTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCgg                                                                                              <  2:1252316/150‑1 (MQ=255)
                                                     aTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCgg                                                                                              >  1:4172550/1‑150 (MQ=255)
                                                       gCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGTAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTc                                                                                            >  2:4031732/1‑150 (MQ=255)
                                                        cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                           >  2:224652/1‑150 (MQ=255)
                                                        cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                           >  2:1273902/1‑150 (MQ=255)
                                                        cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGACACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                           >  2:1815108/1‑150 (MQ=255)
                                                        cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                           <  2:2998119/150‑1 (MQ=255)
                                                         aCCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAg                                                                                          <  2:4496363/150‑1 (MQ=255)
                                                            cATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAgcgc                                                                                       >  1:49777/1‑150 (MQ=255)
                                                            cATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAgcgc                                                                                       <  2:2031504/150‑1 (MQ=255)
                                                            cATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAgcgc                                                                                       >  1:3196784/1‑150 (MQ=255)
                                                                   ccATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAgg                                                                                >  1:1341176/1‑150 (MQ=255)
                                                                     aTTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTc                                                                              >  1:1755416/1‑150 (MQ=255)
                                                                      ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                             <  1:183987/150‑1 (MQ=255)
                                                                      ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                             <  1:185073/150‑1 (MQ=255)
                                                                       tGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAg                                                                            <  1:1965232/150‑1 (MQ=255)
                                                                       tGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAg                                                                            <  1:142292/150‑1 (MQ=255)
                                                                        ggCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAgg                                                                           <  2:2379336/150‑1 (MQ=255)
                                                                          cATTGCCAGTCACCGGATCTTCCACACTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                         <  2:1430597/150‑1 (MQ=255)
                                                                          cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                         >  1:3324597/1‑150 (MQ=255)
                                                                             tGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGaaa                                                                      <  2:3686401/150‑1 (MQ=255)
                                                                              gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                     <  2:612749/150‑1 (MQ=255)
                                                                                    cACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTc                                                               <  1:1648884/150‑1 (MQ=255)
                                                                                       cGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGat                                                            >  2:1263763/1‑150 (MQ=255)
                                                                                       cGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAGTAAGGTCAGGCGAAAGGGCGTCGat                                                            >  2:3908664/1‑150 (MQ=255)
                                                                                                     tACCAATCGCAGGCGAGAACATTCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGtt                                              <  2:4150699/150‑1 (MQ=255)
                                                                                                     tACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGtt                                              <  2:3585875/150‑1 (MQ=255)
                                                                                                        cAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTAGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAATGGGCGTCGATATCCACTTCCGGTTTCa                                           >  2:730115/1‑150 (MQ=255)
                                                                                                         aaTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAg                                          >  2:632596/1‑150 (MQ=255)
                                                                                                          aTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGt                                         <  1:3897416/150‑1 (MQ=255)
                                                                                                          aTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGt                                         <  1:446094/150‑1 (MQ=255)
                                                                                                             gCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTggg                                      <  1:249423/150‑1 (MQ=255)
                                                                                                                  cGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcat                                 <  1:4411220/150‑1 (MQ=255)
                                                                                                                   gagaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                <  2:1101474/150‑1 (MQ=255)
                                                                                                                   gagaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                >  1:3816202/1‑150 (MQ=255)
                                                                                                                    agaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGAtcatca                               <  2:2588624/150‑1 (MQ=255)
                                                                                                                      aaCATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACt                             >  2:4076949/1‑150 (MQ=255)
                                                                                                                         aTGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                          <  2:1958513/150‑1 (MQ=255)
                                                                                                                          tGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                         <  2:3752312/150‑1 (MQ=255)
                                                                                                                          tGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                         <  1:1172009/150‑1 (MQ=255)
                                                                                                                          tGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                         <  2:1750104/150‑1 (MQ=255)
                                                                                                                          tGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTg                         <  1:2852766/150‑1 (MQ=255)
                                                                                                                            cGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAg                       <  1:1455814/150‑1 (MQ=255)
                                                                                                                            cGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAg                       <  1:2386613/150‑1 (MQ=255)
                                                                                                                             gACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgt                      <  2:472568/150‑1 (MQ=255)
                                                                                                                                cATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                   >  1:2419195/1‑150 (MQ=255)
                                                                                                                                cATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                   >  2:3446934/1‑150 (MQ=255)
                                                                                                                                  tCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCt                 <  1:1298755/150‑1 (MQ=255)
                                                                                                                                   cGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtg                <  1:2015141/150‑1 (MQ=255)
                                                                                                                                    ggTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgt               >  2:4503691/1‑150 (MQ=255)
                                                                                                                                     gTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt              >  2:791497/1‑150 (MQ=255)
                                                                                                                                     gTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgtt              >  2:791547/1‑150 (MQ=255)
                                                                                                                                         cGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGttgttg          <  2:3509500/150‑1 (MQ=255)
                                                                                                                                         cGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGttgttg          >  1:599865/1‑150 (MQ=255)
                                                                                                                                          gTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGc         >  1:26595/1‑150 (MQ=255)
                                                                                                                                             tttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAc      <  2:789057/150‑1 (MQ=255)
                                                                                                                                                 ccTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGa  >  1:170158/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGA  >  CP009273/1529360‑1529654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑