BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,591,894	C→A	5.5%	pseudogene (351/5323 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,591,894	0	C	A	5.5%	1033.2 / 23.0	273	pseudogene (351/5323 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand): ref base C (133/125); new base A (5/10); total (138/135)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 274 total aligned reads displayed.

TTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTGGTTAAGGAGG  >  CP009273/1591745‑1592042

 ttAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCa                                                                                                                                                     >  1:4015370/1‑150 (MQ=255)

  tAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAg                                                                                                                                                    <  1:153007/150‑1 (MQ=255)

  tAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAg                                                                                                                                                    <  1:2894027/150‑1 (MQ=255)

   aaTGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGc                                                                                                                                                   >  2:1133383/1‑150 (MQ=255)

   aaTGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGc                                                                                                                                                   >  1:2244676/1‑150 (MQ=255)

      gAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTc                                                                                                                                                >  1:4200251/1‑150 (MQ=255)

       aaCCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCTACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTcc                                                                                                                                               <  1:1540725/150‑1 (MQ=255)

       aaCCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTcc                                                                                                                                               <  1:1539921/150‑1 (MQ=255)

        aCCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTccc                                                                                                                                              >  1:2741805/1‑150 (MQ=255)

            gtgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGtt                                                                                                                                          <  2:3676988/150‑1 (MQ=255)

            gtgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGtt                                                                                                                                          <  1:1139630/150‑1 (MQ=255)

             tgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTc                                                                                                                                         >  1:387557/1‑150 (MQ=255)

             tgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTc                                                                                                                                         >  1:387625/1‑150 (MQ=255)

              gCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTcc                                                                                                                                        <  1:1655252/150‑1 (MQ=255)

              gCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTcc                                                                                                                                        <  1:3912248/150‑1 (MQ=255)

                  tAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGAt                                                                                                                                    <  1:3000532/150‑1 (MQ=255)

                   aGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGAtt                                                                                                                                   <  1:2566814/150‑1 (MQ=255)

                        ccTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGAccc                                                                                                                              >  1:1702726/1‑150 (MQ=255)

                          tATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCcac                                                                                                                            >  1:2963468/1‑150 (MQ=255)

                           aTTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCcaga                                                                                                                           >  2:2917797/1‑148 (MQ=255)

                           aTTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCcaca                                                                                                                           <  1:2227425/150‑1 (MQ=255)

                            tttCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACAt                                                                                                                          <  1:1851693/150‑1 (MQ=255)

                               cGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTGAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTa                                                                                                                       <  2:1719523/150‑1 (MQ=255)

                                    aatTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGc                                                                                                                  >  2:3815655/1‑150 (MQ=255)

                                    aatTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGc                                                                                                                  >  2:3969669/1‑150 (MQ=255)

                                      ttCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCt                                                                                                                <  2:1084017/150‑1 (MQ=255)

                                       tCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCtt                                                                                                               >  2:2014112/1‑150 (MQ=255)

                                       tCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCtt                                                                                                               >  2:2504060/1‑150 (MQ=255)

                                        cGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTg                                                                                                              >  2:2958084/1‑150 (MQ=255)

                                          gtcgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTATGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCa                                                                                                            >  2:3790600/1‑150 (MQ=255)

                                           tcgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCat                                                                                                           <  1:3969791/150‑1 (MQ=255)

                                           tcgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGACTACTGTCGAAGAACCTCATCTTAAATAGCCTCCATCTACGTGACCCTTCTGTTTAATATTCAGCTTCCCGGTTCCGGATTGACCCACATTTAAATGCCTTGCat                                                                                                           <  2:1905881/150‑1 (MQ=255)

                                            cgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCatt                                                                                                          <  1:1545767/150‑1 (MQ=255)

                                            cgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCatt                                                                                                          <  1:4214741/150‑1 (MQ=255)

                                             gtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCatta                                                                                                         <  2:3418043/150‑1 (MQ=255)

                                               cAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCattatt                                                                                                       >  1:1323046/1‑150 (MQ=255)

                                                aGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTAttt                                                                                                      >  1:2842475/1‑150 (MQ=255)

                                                 gAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTc                                                                                                     >  2:651410/1‑150 (MQ=255)

                                                     agagTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCt                                                                                                 >  2:4457814/1‑150 (MQ=255)

                                                       agTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTAt                                                                                               >  1:2124653/1‑150 (MQ=255)

                                                          ccTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTACTCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCAt                                                                                            <  2:3554726/150‑1 (MQ=255)

                                                          ccTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGACTTGCATTATTTCCGCTATCAt                                                                                            >  2:4048613/1‑150 (MQ=255)

                                                            tctcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATAc                                                                                          <  2:852263/150‑1 (MQ=255)

                                                             ctcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACa                                                                                         >  1:421839/1‑150 (MQ=255)

                                                                ccTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATc                                                                                      <  1:382328/150‑1 (MQ=255)

                                                                 cTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCg                                                                                     <  1:990407/150‑1 (MQ=255)

                                                                 cTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCg                                                                                     <  1:344685/150‑1 (MQ=255)

                                                                       aTTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGt                                                                               <  2:4310533/150‑1 (MQ=255)

                                                                       aTTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGt                                                                               >  2:3025381/1‑150 (MQ=255)

                                                                       aTTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGt                                                                               <  2:1849214/150‑1 (MQ=255)

                                                                                cccGACGCCTCCTGTCGAAGATCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCaa                                                                      <  2:4535478/150‑1 (MQ=255)

                                                                                cccGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCaa                                                                      <  2:1830576/150‑1 (MQ=255)

                                                                                   gACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAAc                                                                   >  2:2081868/1‑150 (MQ=255)

                                                                                   gACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCGTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAAc                                                                   >  2:1361704/1‑150 (MQ=255)

                                                                                     cGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACAt                                                                 >  1:1004473/1‑150 (MQ=255)

                                                                                     cGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACAt                                                                 <  1:3233040/150‑1 (MQ=255)

                                                                                        ctcctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTaa                                                              <  1:587594/150‑1 (MQ=255)

                                                                                        ctcctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTaa                                                              >  2:4057471/1‑150 (MQ=255)

                                                                                          cctGTCGAATAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCAAGTTCCTTATTGACACACATTTAAAGGCCTTGCATTATTTCCTCTATCATAAAATCGCCAGGTGCCACCCAAAACATTAACg                                                            <  2:4010280/150‑1 (MQ=255)

                                                                                           ctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACgg                                                           <  2:3452889/150‑1 (MQ=255)

                                                                                           ctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACgg                                                           <  2:1049287/150‑1 (MQ=255)

                                                                                            tGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATATCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGt                                                          >  2:1150336/1‑150 (MQ=255)

                                                                                             gTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTg                                                         >  2:3258555/1‑150 (MQ=255)

                                                                                              tCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGc                                                        <  1:3030730/150‑1 (MQ=255)

                                                                                              tCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGc                                                        >  1:3832771/1‑150 (MQ=255)

                                                                                               cGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGcc                                                       >  1:937596/1‑150 (MQ=255)

                                                                                                gaagaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGccc                                                      >  1:3200677/1‑150 (MQ=255)

                                                                                                 aagaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCACAACATTAACGGTGCCct                                                     >  2:648202/1‑150 (MQ=255)

                                                                                                  agaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctc                                                    >  2:1958859/1‑150 (MQ=255)

                                                                                                          aTCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCt                                            >  2:2743209/1‑150 (MQ=255)

                                                                                                           tCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTc                                           <  2:1407664/150‑1 (MQ=255)

                                                                                                               aaaTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTa                                       <  2:3125176/150‑1 (MQ=255)

                                                                                                               aaaTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTa                                       <  2:4260563/150‑1 (MQ=255)

                                                                                                               aaaTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTa                                       >  1:946493/1‑150 (MQ=255)

                                                                                                               aaaTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTa                                       <  2:1160396/150‑1 (MQ=255)

                                                                                                                 aTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAgc                                     >  2:973463/1‑150 (MQ=255)

                                                                                                                 aTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAgc                                     >  2:972805/1‑150 (MQ=255)

                                                                                                                 aTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAgc                                     <  2:4290552/150‑1 (MQ=255)

                                                                                                                 aTAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAgc                                     <  1:4487221/150‑1 (MQ=255)

                                                                                                                     ccTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCa                                 >  1:4346060/1‑150 (MQ=255)

                                                                                                                     ccTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCa                                 >  2:568818/1‑150 (MQ=255)

                                                                                                                     ccTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCAGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCa                                 <  2:2126326/150‑1 (MQ=255)

                                                                                                                      cTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCac                                >  2:2784645/1‑149 (MQ=255)

                                                                                                                      cTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTGGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCaa                                <  1:2081868/150‑1 (MQ=255)

                                                                                                                       tCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAAt                               >  1:2895025/1‑150 (MQ=255)

                                                                                                                          aTCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGAc                            >  2:2454250/1‑150 (MQ=255)

                                                                                                                            cGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGc                          >  2:3278380/1‑150 (MQ=255)

                                                                                                                              aCGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTg                        <  1:1414310/150‑1 (MQ=255)

                                                                                                                              aCGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTg                        <  1:3678786/150‑1 (MQ=255)

                                                                                                                                gTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGgt                      <  2:4340912/150‑1 (MQ=255)

                                                                                                                                  gACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGgtag                    >  2:2251489/1‑150 (MQ=255)

                                                                                                                                   aCCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGgtagt                   <  2:387794/150‑1 (MQ=255)

                                                                                                                                      cTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAAt                <  1:1452354/150‑1 (MQ=255)

                                                                                                                                      cTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAAt                <  1:881493/150‑1 (MQ=255)

                                                                                                                                          tGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTg            >  1:838576/1‑150 (MQ=255)

                                                                                                                                           gTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTgg           >  1:1800915/1‑150 (MQ=255)

                                                                                                                                            tttAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTGGt          <  2:4200251/150‑1 (MQ=255)

                                                                                                                                             ttAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCACCAATGACGCTGGTAGTAATAGTGGtt         >  1:2951216/1‑150 (MQ=255)

                                                                                                                                                   ttCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTGGTTAaggag   <  2:1325626/150‑1 (MQ=255)

                                                                                                                                                   ttCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTGGTTAaggag   >  2:831899/1‑150 (MQ=255)

TTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTCATTAGCGCCAATGACGCTGGTAGTAATAGTGGTTAAGGAGG  >  CP009273/1591745‑1592042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`