BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,610,766	C→T	42.9%	S249S (AGC→AGT)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,610,766	0	C	T	42.9%	138.0 / inf	254	S249S (AGC→AGT)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (67/78); new base T (53/56); total (120/134)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 255 total aligned reads displayed.

ATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGA  >  CP009273/1610618‑1610914

aTTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGcc                                                                                                                                                     <  1:1736837/150‑1 (MQ=255)

 ttttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCt                                                                                                                                                    >  1:3721525/1‑150 (MQ=255)

   ttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGa                                                                                                                                                  <  2:1654618/150‑1 (MQ=255)

     aTCTCTCGCGGTAATTTTGTAATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATg                                                                                                                                                <  2:2468827/150‑1 (MQ=255)

      tctctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATgg                                                                                                                                               >  1:4277232/1‑150 (MQ=255)

        tctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGAt                                                                                                                                             >  1:694009/1‑150 (MQ=255)

        tctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGAt                                                                                                                                             >  1:2644536/1‑150 (MQ=255)

               gTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAg                                                                                                                                      >  1:23030/1‑150 (MQ=255)

                tAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAgg                                                                                                                                     >  2:385175/1‑150 (MQ=255)

                tAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAgg                                                                                                                                     >  2:1816884/1‑150 (MQ=255)

                 aaTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGt                                                                                                                                    >  2:3354142/1‑150 (MQ=255)

                 aaTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGt                                                                                                                                    >  1:3960010/1‑150 (MQ=255)

                   ttttGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTc                                                                                                                                  <  1:1376372/150‑1 (MQ=255)

                         cATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTaacaa                                                                                                                            >  2:2043371/1‑150 (MQ=255)

                          aTACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTaacaaa                                                                                                                           >  1:2426970/1‑150 (MQ=255)

                          aTACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaacaaa                                                                                                                           >  1:4030926/1‑150 (MQ=255)

                                     aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                                <  1:996623/150‑1 (MQ=255)

                                     aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                                >  1:956365/1‑150 (MQ=255)

                                     aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                                <  1:4036082/150‑1 (MQ=255)

                                     aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                                >  1:814942/1‑150 (MQ=255)

                                     aTTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTAcc                                                                                                                <  2:132406/150‑1 (MQ=255)

                                      ttATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTAccc                                                                                                               <  2:2223589/150‑1 (MQ=255)

                                      ttATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTAccc                                                                                                               <  2:1471749/150‑1 (MQ=255)

                                         tGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGaa                                                                                                            >  1:3240723/1‑150 (MQ=255)

                                          ggAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAg                                                                                                           <  1:2023054/150‑1 (MQ=255)

                                             aTAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCt                                                                                                        <  1:2896471/150‑1 (MQ=255)

                                               aaCTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGc                                                                                                      <  2:3043290/150‑1 (MQ=255)

                                                 ctctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACGTGATCACCGCCATTCTCGACATAACGGACAGCGACTTAAAAGAGGATGTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCAt                                                                                                    >  2:2164296/1‑150 (MQ=255)

                                                  tctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATc                                                                                                   <  2:1127/150‑1 (MQ=255)

                                                  tctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACAAAAAGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATc                                                                                                   <  1:4437594/150‑1 (MQ=255)

                                                    tGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTg                                                                                                 >  1:903435/1‑150 (MQ=255)

                                                     gAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGc                                                                                                <  2:4095987/150‑1 (MQ=255)

                                                      aGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCt                                                                                               >  2:2106799/1‑150 (MQ=255)

                                                        gTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttt                                                                                             <  2:4105038/150‑1 (MQ=255)

                                                           cacaGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTa                                                                                          >  2:1573828/1‑150 (MQ=255)

                                                            anaGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTAt                                                                                         >  1:1191592/1‑150 (MQ=255)

                                                            acaGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTAt                                                                                         >  2:3726998/1‑150 (MQ=255)

                                                                aTGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCt                                                                                     >  1:3194389/1‑150 (MQ=255)

                                                                    ttCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTa                                                                                 <  2:2514808/150‑1 (MQ=255)

                                                                     tCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAg                                                                                <  1:2818425/150‑1 (MQ=255)

                                                                          aaTAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACTGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGaa                                                                           >  1:4438093/1‑150 (MQ=255)

                                                                          aaTAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGaa                                                                           >  1:515378/1‑150 (MQ=255)

                                                                           aTAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAAc                                                                          <  1:603179/150‑1 (MQ=255)

                                                                             aaaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACaa                                                                        <  1:1197025/150‑1 (MQ=255)

                                                                             aaaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACaa                                                                        >  2:4161625/1‑150 (MQ=255)

                                                                              aaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAg                                                                       >  1:73482/1‑150 (MQ=255)

                                                                              aaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAg                                                                       <  2:1693178/150‑1 (MQ=255)

                                                                                  tCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtc                                                                   <  1:2737051/150‑1 (MQ=255)

                                                                                      aaCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGTTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                               >  1:2054897/1‑150 (MQ=255)

                                                                                       aCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                              <  2:1913481/150‑1 (MQ=255)

                                                                                        cTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCa                                                             <  2:3277046/150‑1 (MQ=255)

                                                                                        cTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCa                                                             <  1:2457860/150‑1 (MQ=255)

                                                                                         ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                              >  2:226348/1‑148 (MQ=255)

                                                                                         ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                              <  1:226331/148‑1 (MQ=255)

                                                                                         ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                              <  1:226402/148‑1 (MQ=255)

                                                                                         ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcctc                                                              <  1:2523607/148‑1 (MQ=255)

                                                                                         ttGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAt                                                            <  1:2660806/150‑1 (MQ=255)

                                                                                          agATCACCTACATTCTCGAAATAAAGGACAGAGAATTAAAAGATGATTTGTTTCAAAGTCTGATGGATTGATTAGGTTATAACAAACAGGTAGTACCCGAATTCTGCATCTGATTTTTATCCCTTTTAGCAGAAATAGATTCTCCTCAtt                                                           <  2:3657991/149‑1 (MQ=255)

                                                                                           gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                          >  2:4166506/1‑150 (MQ=255)

                                                                                             tCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTc                                                        <  1:4161713/150‑1 (MQ=255)

                                                                                             tCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTc                                                        <  2:2617824/150‑1 (MQ=255)

                                                                                              cACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCa                                                       <  2:4195872/150‑1 (MQ=255)

                                                                                                 cGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGaa                                                    <  2:490030/150‑1 (MQ=255)

                                                                                                  gCCATTCTCGACATAACGGACAGCGAATTAATAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCACTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                   <  1:2201326/150‑1 (MQ=255)

                                                                                                   ccATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATCTTCAGAACt                                                  >  2:953782/1‑150 (MQ=255)

                                                                                                        ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                             >  1:525529/1‑150 (MQ=255)

                                                                                                        ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                             >  2:2475075/1‑150 (MQ=255)

                                                                                                         tcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGtttt                                            >  1:3230800/1‑150 (MQ=255)

                                                                                                          cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                           >  1:104179/1‑150 (MQ=255)

                                                                                                          cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                           >  1:2180842/1‑150 (MQ=255)

                                                                                                              aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                       >  1:4536655/1‑150 (MQ=255)

                                                                                                              aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                       <  2:2811636/150‑1 (MQ=255)

                                                                                                                aaCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttttt                                     <  2:2741499/150‑1 (MQ=255)

                                                                                                                aaCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttttt                                     >  2:2605207/1‑150 (MQ=255)

                                                                                                                 aCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTc                                    <  1:1027147/150‑1 (MQ=255)

                                                                                                                  cGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCg                                   <  2:768778/150‑1 (MQ=255)

                                                                                                                    gACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGcc                                 >  1:3304071/1‑150 (MQ=255)

                                                                                                                     aCAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCa                                >  1:4132653/1‑150 (MQ=255)

                                                                                                                       aGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAat                              >  1:1510400/1‑150 (MQ=255)

                                                                                                                        gCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAata                             <  2:3240723/150‑1 (MQ=255)

                                                                                                                         cGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAatat                            >  1:203409/1‑150 (MQ=255)

                                                                                                                           aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                          >  1:3739300/1‑150 (MQ=255)

                                                                                                                             ttAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTGCTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTa                        <  1:3865667/150‑1 (MQ=255)

                                                                                                                                 aaGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGc                    >  1:2978228/1‑150 (MQ=255)

                                                                                                                                 aaGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGc                    >  1:2979961/1‑150 (MQ=255)

                                                                                                                                  agagGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCa                   <  1:986248/150‑1 (MQ=255)

                                                                                                                                  agagGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCa                   <  1:782559/150‑1 (MQ=255)

                                                                                                                                     ggATTTGTTGCAAAGTCTGATTGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTa                <  2:4337231/150‑1 (MQ=255)

                                                                                                                                      gATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTAt               >  1:4388088/1‑150 (MQ=255)

                                                                                                                                         ttgttgCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCAc            >  1:1870711/1‑150 (MQ=255)

                                                                                                                                          tgttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCAcc           >  1:3413576/1‑150 (MQ=255)

                                                                                                                                           gttgCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCa          <  1:1465569/150‑1 (MQ=255)

                                                                                                                                           gttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCa          <  1:3018836/150‑1 (MQ=255)

                                                                                                                                            ttgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCaa         >  2:2379969/1‑150 (MQ=255)

                                                                                                                                             tgCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAt        <  2:580843/150‑1 (MQ=255)

                                                                                                                                             tgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAt        <  2:4438093/150‑1 (MQ=255)

                                                                                                                                              gCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAtt       <  1:3882362/150‑1 (MQ=255)

                                                                                                                                              gCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAtt       <  1:3883104/150‑1 (MQ=255)

                                                                                                                                              gCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAtt       >  2:4519120/1‑150 (MQ=255)

                                                                                                                                                   gCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGa  <  1:2154215/150‑1 (MQ=255)

ATTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGA  >  CP009273/1610618‑1610914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`