Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,661,763 C→A 100% P353P (CCG→CCT)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,661,7630CA97.3% 905.7 / 11.8 276P353P (CCG→CCT) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (136/132);  total (140/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

GAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAA  >  CP009273/1661618‑1661909
                                                                                                                                                 |                                                                                                                                                  
 aaTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGaa                                                                                                                                               <  2:3219658/150‑1 (MQ=255)
  aTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAag                                                                                                                                              >  2:290264/1‑150 (MQ=255)
       caaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGat                                                                                                                                         <  2:312472/150‑1 (MQ=255)
       caaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGat                                                                                                                                         >  2:3954761/1‑150 (MQ=255)
        aaaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGata                                                                                                                                        >  1:507323/1‑150 (MQ=255)
        aaaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGata                                                                                                                                        >  1:2391169/1‑150 (MQ=255)
          aGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGATAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCTGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGatatg                                                                                                                                      <  2:1150055/150‑2 (MQ=255)
           gAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCt                                                                                                                                     >  2:1795185/1‑150 (MQ=255)
             aCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGACGAGGATATCTGc                                                                                                                                   >  1:2436268/1‑150 (MQ=255)
             aCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGc                                                                                                                                   <  2:2606721/150‑1 (MQ=255)
              ccGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGcc                                                                                                                                  <  1:3408342/150‑1 (MQ=255)
               cGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCg                                                                                                                                 >  1:3811966/1‑150 (MQ=255)
               cGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCg                                                                                                                                 >  1:3812030/1‑150 (MQ=255)
                gTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGc                                                                                                                                >  1:1568747/1‑150 (MQ=255)
                 ttATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCt                                                                                                                               >  2:1874036/1‑150 (MQ=255)
                  tataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCtt                                                                                                                              >  1:3340906/1‑150 (MQ=255)
                    taCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTa                                                                                                                            <  1:284905/150‑1 (MQ=255)
                     aCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATCCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTAc                                                                                                                           <  2:1309311/150‑1 (MQ=255)
                         cgcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGTGGATATATGCCGCTTTACTTaa                                                                                                                       >  2:1137699/1‑150 (MQ=255)
                          gcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAc                                                                                                                      >  1:4303676/1‑150 (MQ=255)
                             tCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGt                                                                                                                   <  1:3230797/150‑1 (MQ=255)
                               ttttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGa                                                                                                                 <  2:4074965/150‑1 (MQ=255)
                                   aCCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCa                                                                                                             <  1:1792636/150‑1 (MQ=255)
                                   aCCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCa                                                                                                             <  1:3728538/150‑1 (MQ=255)
                                    ccAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCaa                                                                                                            >  1:2725780/1‑150 (MQ=255)
                                      aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATc                                                                                                          >  2:4094514/1‑150 (MQ=255)
                                        tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                        <  1:824104/150‑1 (MQ=255)
                                        tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                        >  2:378481/1‑150 (MQ=255)
                                         gCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGt                                                                                                       >  2:3440881/1‑150 (MQ=255)
                                          cAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTa                                                                                                      <  2:467250/150‑1 (MQ=255)
                                          cAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTa                                                                                                      >  1:3483526/1‑150 (MQ=255)
                                              gcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttt                                                                                                  <  1:3642679/150‑1 (MQ=255)
                                               ccTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttttt                                                                                                 <  2:1056524/150‑1 (MQ=255)
                                                cTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttttt                                                                                                >  2:2466356/1‑150 (MQ=255)
                                                 tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTtg                                                                                               <  1:2194934/150‑1 (MQ=255)
                                                         tGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaa                                                                                       >  1:3021577/1‑150 (MQ=255)
                                                         tGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaa                                                                                       >  1:2227427/1‑150 (MQ=255)
                                                          gCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGATGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaa                                                                                      <  1:2106496/150‑1 (MQ=255)
                                                            ccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaata                                                                                    <  2:2391169/150‑1 (MQ=255)
                                                              tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                  >  1:1694863/1‑150 (MQ=255)
                                                                gTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATATAtt                                                                                <  1:1057902/150‑1 (MQ=255)
                                                                gTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAtt                                                                                <  1:1472922/150‑1 (MQ=255)
                                                                   aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                             <  1:1939248/150‑1 (MQ=255)
                                                                   aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                             >  2:2222811/1‑150 (MQ=255)
                                                                     aaaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                           >  2:1907248/1‑150 (MQ=255)
                                                                     aaaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                           >  1:844693/1‑150 (MQ=255)
                                                                      aaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCat                                                                          >  1:2623493/1‑150 (MQ=255)
                                                                         cTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgat                                                                       >  2:2560125/1‑150 (MQ=255)
                                                                         cTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgat                                                                       <  1:3908937/150‑1 (MQ=255)
                                                                           gAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATgg                                                                     >  1:1093027/1‑150 (MQ=255)
                                                                              tACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCaa                                                                  >  1:3466839/1‑150 (MQ=255)
                                                                               aCTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCTCCATGATGGCAAg                                                                 >  2:4450409/1‑150 (MQ=255)
                                                                               aCTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAg                                                                 >  2:3539562/1‑150 (MQ=255)
                                                                               aCTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAg                                                                 >  2:3539570/1‑150 (MQ=255)
                                                                               aCTCTCAACGCTGATGTGATGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACATAACGGTGAGCCAATCAGTATTTTTTTTTGGTTAAATAAATTCACAATTATGGCAAg                                                                 >  2:3539534/1‑150 (MQ=255)
                                                                                ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGa                                                                >  2:2022543/1‑150 (MQ=255)
                                                                                ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGa                                                                <  1:1937872/150‑1 (MQ=255)
                                                                                 tctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGaa                                                               >  2:4006378/1‑150 (MQ=255)
                                                                                 tctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGaa                                                               >  1:3043772/1‑150 (MQ=255)
                                                                                 tctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCGGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGaa                                                               <  1:4026314/150‑1 (MQ=255)
                                                                                   tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                             >  2:4086337/1‑150 (MQ=255)
                                                                                   tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                             >  2:2084503/1‑150 (MQ=255)
                                                                                   tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                             >  2:3636753/1‑150 (MQ=255)
                                                                                   tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                             >  2:1372580/1‑150 (MQ=255)
                                                                                    cAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                            >  1:4045513/1‑150 (MQ=255)
                                                                                    cAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                            <  1:4226995/150‑1 (MQ=255)
                                                                                      aCGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcgc                                                          <  1:2174702/150‑1 (MQ=255)
                                                                                        gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                        <  2:1699265/150‑1 (MQ=255)
                                                                                        gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                        <  2:1448905/150‑1 (MQ=255)
                                                                                           gATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGAc                                                     <  1:4274236/150‑1 (MQ=255)
                                                                                             tgtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACAt                                                   <  2:2725783/150‑1 (MQ=255)
                                                                                                  tGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgcg                                              <  1:290340/150‑1 (MQ=255)
                                                                                                    aCTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                            <  1:1047458/150‑1 (MQ=255)
                                                                                                    aCTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                            <  2:3834839/150‑1 (MQ=255)
                                                                                                      tataCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCa                                          >  1:1428609/1‑150 (MQ=255)
                                                                                                         aCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAccc                                       <  2:3811966/150‑1 (MQ=255)
                                                                                                          cGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                      >  2:43991/1‑150 (MQ=255)
                                                                                                           gCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                                     >  1:2054785/1‑150 (MQ=255)
                                                                                                             aGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGt                                   >  2:42977/1‑150 (MQ=255)
                                                                                                             aGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGt                                   <  1:436929/150‑1 (MQ=255)
                                                                                                                 aGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                               >  2:3734083/1‑150 (MQ=255)
                                                                                                                 aGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                               <  1:2488798/150‑1 (MQ=255)
                                                                                                                 aGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                               >  1:2079540/1‑150 (MQ=255)
                                                                                                                 aGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                               <  2:2346858/150‑1 (MQ=255)
                                                                                                                  gggCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatga                              <  1:3126448/150‑1 (MQ=255)
                                                                                                                   ggCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                             >  2:83364/1‑150 (MQ=255)
                                                                                                                   ggCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                             >  2:767253/1‑150 (MQ=255)
                                                                                                                    gCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgatg                            <  1:2268833/150‑1 (MQ=255)
                                                                                                                       tgctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                         >  1:370749/1‑150 (MQ=255)
                                                                                                                       tgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                         <  1:3330266/150‑1 (MQ=255)
                                                                                                                       tgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                         <  1:3985189/150‑1 (MQ=255)
                                                                                                                       tgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTAACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                         <  2:854686/150‑1 (MQ=255)
                                                                                                                       tgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGATTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTAACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCt                         <  2:4037517/150‑1 (MQ=255)
                                                                                                                          tgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt                      <  2:2655180/150‑1 (MQ=255)
                                                                                                                             cGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTTGGTTAAATAAATTCACCATGATGGCACGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGcc                   >  2:4193483/1‑150 (MQ=255)
                                                                                                                                  gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg              <  2:3344266/150‑1 (MQ=255)
                                                                                                                                    tGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGAt            >  2:1687813/1‑150 (MQ=255)
                                                                                                                                     gTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATc           <  1:4331362/150‑1 (MQ=255)
                                                                                                                                       cTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgc         >  2:2134772/1‑150 (MQ=255)
                                                                                                                                         gagaTGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccg       >  1:1921006/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAA  >  CP009273/1661618‑1661909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑