Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,689,180 T→G 62.1% E383D (GAA→GAC)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,689,1800TG62.1% 335.0 / 245.1 242E383D (GAA→GAC) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base G (59/91);  total (102/140)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

GCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCA  >  CP009273/1689032‑1689327
                                                                                                                                                    |                                                                                                                                                   
gcgTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGttt                                                                                                                                                    <  1:800893/150‑1 (MQ=255)
gcgTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGttt                                                                                                                                                    <  1:800952/150‑1 (MQ=255)
  gTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTcc                                                                                                                                                  <  1:3011234/150‑1 (MQ=255)
          cGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGAc                                                                                                                                          >  1:2328677/1‑150 (MQ=255)
               gTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCct                                                                                                                                     >  1:3125018/1‑150 (MQ=255)
               gTGGCGCGAAATATTCCCGAGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCct                                                                                                                                     >  1:3924612/1‑150 (MQ=255)
                tGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCctc                                                                                                                                    >  2:1338980/1‑150 (MQ=255)
                 ggCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCctct                                                                                                                                   <  2:2494725/150‑1 (MQ=255)
                  gcgcGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCtt                                                                                                                                  >  1:1616357/1‑150 (MQ=255)
                  gcgcGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCtt                                                                                                                                  <  2:413154/150‑1 (MQ=255)
                   cgcgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTc                                                                                                                                 <  1:2325834/150‑1 (MQ=255)
                   cgcgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTc                                                                                                                                 <  1:1559498/150‑1 (MQ=255)
                    gcgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTTGCTATACTCCACATCTCCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCttag                                                                                                                                <  2:4165400/150‑3 (MQ=255)
                    gcgAAATATTCCCGTGCACCGTGCGGACGGCTATCCGGTTAGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCg                                                                                                                                <  2:2913022/150‑1 (MQ=255)
                     cgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGc                                                                                                                               >  1:431747/1‑150 (MQ=255)
                      gAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCt                                                                                                                              >  1:617111/1‑150 (MQ=255)
                           atTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                         <  2:57337/150‑1 (MQ=255)
                           atTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                         >  1:2056004/1‑150 (MQ=255)
                            gtCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAg                                                                                                                        <  2:1339549/149‑1 (MQ=255)
                             tCCCGTGCACCGTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGt                                                                                                                       <  2:3931577/150‑1 (MQ=255)
                              cccGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGtt                                                                                                                      <  2:1391162/150‑1 (MQ=255)
                                 gTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCtt                                                                                                                   <  1:4244849/150‑1 (MQ=255)
                                  tGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCttt                                                                                                                  <  1:1989189/150‑1 (MQ=255)
                                  tGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCttt                                                                                                                  <  1:1989126/150‑1 (MQ=255)
                                  tGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCttt                                                                                                                  <  1:3489730/150‑1 (MQ=255)
                                     aCCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCgg                                                                                                               <  1:436864/150‑1 (MQ=255)
                                     aCCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCgg                                                                                                               <  1:436851/150‑1 (MQ=255)
                                     aCCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCgg                                                                                                               <  1:436806/150‑1 (MQ=255)
                                     aCCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCgg                                                                                                               >  1:3339781/1‑150 (MQ=255)
                                       cTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGATTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCt                                                                                                             >  2:4368978/1‑150 (MQ=255)
                                         tGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttg                                                                                                           >  2:1507000/1‑150 (MQ=255)
                                           cGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgtt                                                                                                         <  2:1089261/150‑1 (MQ=255)
                                            ggACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgttg                                                                                                        >  1:2163218/1‑150 (MQ=255)
                                            ggACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgttg                                                                                                        >  2:925330/1‑150 (MQ=255)
                                              aCGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGcc                                                                                                      <  2:3587116/150‑1 (MQ=255)
                                               cGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGccc                                                                                                     <  2:2730874/150‑1 (MQ=255)
                                               cGGGTATCCGGTTCGTTGGCAAGACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGccc                                                                                                     <  2:3598003/150‑1 (MQ=255)
                                                gggTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCg                                                                                                    >  1:2894385/1‑150 (MQ=255)
                                                gggTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCg                                                                                                    <  1:4315495/150‑1 (MQ=255)
                                                     tCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCg                                                                                               <  2:4308386/150‑1 (MQ=255)
                                                         gTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAc                                                                                           >  2:1186274/1‑150 (MQ=255)
                                                          ttCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAcc                                                                                          >  1:2742204/1‑150 (MQ=255)
                                                          ttCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAcc                                                                                          >  1:2741802/1‑150 (MQ=255)
                                                          ttCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAcc                                                                                          <  2:3189690/150‑1 (MQ=255)
                                                               tGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGc                                                                                     >  1:3861002/1‑150 (MQ=255)
                                                               tGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGc                                                                                     >  2:1139534/1‑150 (MQ=255)
                                                                ggCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGcc                                                                                    <  2:3858012/150‑1 (MQ=255)
                                                                ggCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGcc                                                                                    <  2:3858234/150‑1 (MQ=255)
                                                                 gCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCt                                                                                   <  1:3038840/150‑1 (MQ=255)
                                                                  cAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTa                                                                                  >  2:2979000/1‑150 (MQ=255)
                                                                  cAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTa                                                                                  >  2:37362/1‑150 (MQ=255)
                                                                    aTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaaa                                                                                <  1:2691580/150‑1 (MQ=255)
                                                                    aTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaaa                                                                                >  1:2505662/1‑150 (MQ=255)
                                                                    aTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaaa                                                                                >  1:2505647/1‑150 (MQ=255)
                                                                      aCTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAaga                                                                              <  1:4133803/150‑1 (MQ=255)
                                                                       cTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagag                                                                             <  1:2846153/150‑1 (MQ=255)
                                                                        tCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                            <  2:152039/150‑1 (MQ=255)
                                                                            caTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGtt                                                                        <  1:4153111/150‑1 (MQ=255)
                                                                            caTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGtt                                                                        >  1:1419512/1‑150 (MQ=255)
                                                                             aTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTa                                                                       <  2:750154/150‑1 (MQ=255)
                                                                                accacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAg                                                                    <  1:992048/150‑1 (MQ=255)
                                                                                accacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAg                                                                    <  1:3268350/150‑1 (MQ=255)
                                                                                 ccacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGc                                                                   <  1:71837/150‑1 (MQ=255)
                                                                                  cacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                                  <  2:239768/150‑1 (MQ=255)
                                                                                  cacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                                  >  1:3845360/1‑150 (MQ=255)
                                                                                  cacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                                  <  1:3488555/150‑1 (MQ=255)
                                                                                      cTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAca                                                              <  2:3596272/150‑1 (MQ=255)
                                                                                       ttGGGTGGTTTTTGTCAAGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACGGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcag                                                             >  1:4432466/1‑150 (MQ=255)
                                                                                         gggTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCTCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGACAGCTTCGAAACCAATGACTAAAGAGAGGTTAAAGCCGAcagca                                                           >  1:110726/1‑150 (MQ=255)
                                                                                             ggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGATACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagcag                                                       <  1:1510790/150‑1 (MQ=255)
                                                                                               tttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGtt                                                     <  2:3917198/150‑1 (MQ=255)
                                                                                                    gTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtcatc                                                <  1:3758108/150‑1 (MQ=255)
                                                                                                           gcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTAGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcacc                                         >  2:930119/1‑150 (MQ=255)
                                                                                                             tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                       <  1:1487876/150‑1 (MQ=255)
                                                                                                             tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                       >  2:4125955/1‑150 (MQ=255)
                                                                                                                   ctctTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGcc                                 <  2:3713796/150‑1 (MQ=255)
                                                                                                                      tttAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGTCTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATg                              >  1:3692489/1‑150 (MQ=255)
                                                                                                                        tAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGtt                            <  2:1841316/150‑1 (MQ=255)
                                                                                                                        tAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGtt                            <  1:4065158/150‑1 (MQ=255)
                                                                                                                        tAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGtt                            <  1:1257485/150‑1 (MQ=255)
                                                                                                                          aTCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCa                          >  2:3352958/1‑150 (MQ=255)
                                                                                                                          aTCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCa                          <  1:1070715/150‑1 (MQ=255)
                                                                                                                            cGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATc                        <  1:2185522/150‑1 (MQ=255)
                                                                                                                             gCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCt                       <  2:2328677/150‑1 (MQ=255)
                                                                                                                             gCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTAGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCt                       <  1:262027/150‑1 (MQ=255)
                                                                                                                                tGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGcc                    >  2:2142900/1‑150 (MQ=255)
                                                                                                                                 gTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGccc                   <  1:1804717/150‑1 (MQ=255)
                                                                                                                                 gTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGccc                   >  1:3132686/1‑150 (MQ=255)
                                                                                                                                  tAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCa                  <  1:43599/150‑1 (MQ=255)
                                                                                                                                     gTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTc               >  1:706980/1‑150 (MQ=255)
                                                                                                                                      tGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGTAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCg              >  2:40498/1‑150 (MQ=255)
                                                                                                                                        cgcTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAg            >  2:3238668/1‑150 (MQ=255)
                                                                                                                                        cgcTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAg            <  1:2244796/150‑1 (MQ=255)
                                                                                                                                            tGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAtc        >  1:2974803/1‑150 (MQ=255)
                                                                                                                                             gCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAtct       <  2:706980/150‑1 (MQ=255)
                                                                                                                                              cTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTCCCCAGTCGAGCAtctc      <  1:4125955/150‑1 (MQ=255)
                                                                                                                                               tGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATctct     <  2:2590168/150‑1 (MQ=255)
                                                                                                                                                gAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCtt    >  1:1697781/1‑150 (MQ=255)
                                                                                                                                                 aGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTc   >  1:2872130/1‑150 (MQ=255)
                                                                                                                                                  gTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCa  >  2:2352139/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCA  >  CP009273/1689032‑1689327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑