Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,726,437 C→A 6.8% L1032I (CTC→ATC)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,726,4370CA 6.8% 825.1 / 25.8 220L1032I (CTC→ATC) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (106/99);  new base A (8/7);  total (114/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 228 total aligned reads displayed.

CAGCGATGATTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAGTATTT  >  CP009273/1726288‑1726586
                                                                                                                                                     |                                                                                                                                                     
cAGCGATGATTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTAtc                                                                                                                                                       >  1:2587757/1‑150 (MQ=255)
 aGCGATGATTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTAtct                                                                                                                                                      <  1:3238276/150‑1 (MQ=255)
 aGCGATGATTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTAtct                                                                                                                                                      >  1:3522564/1‑150 (MQ=255)
    gatgatTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCg                                                                                                                                                   >  1:3811496/1‑150 (MQ=255)
      tgatTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCggg                                                                                                                                                 >  2:2586899/1‑150 (MQ=255)
       gatTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATATCCGGGc                                                                                                                                                >  1:217202/1‑150 (MQ=255)
        atTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCt                                                                                                                                               >  2:1320952/1‑150 (MQ=255)
             cggttaGGAGAGTCTTGCCTGTATGGATGATGCCGGCAGGCTGCGTGTTGCCCTCGGCGTACGACTTCCAGAGTCATTGCCTGAGATTTTTTTACATAGAGTCAGTTACCCGCTTCGCGACCTATTTCTGCGCTATCTCCGGGCTCATGc                                                                                                                                          <  1:1562585/145‑1 (MQ=255)
              ggACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGct                                                                                                                                         >  2:3662604/1‑150 (MQ=255)
                  aGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTgg                                                                                                                                     >  2:1750786/1‑150 (MQ=255)
                    gAGCGTCTTGCCTGTATGGATTATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTc                                                                                                                                   >  2:1043020/1‑150 (MQ=255)
                        gTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACgg                                                                                                                               >  2:1523197/1‑150 (MQ=255)
                           ttGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTg                                                                                                                            >  1:77058/1‑150 (MQ=255)
                              ccTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGaac                                                                                                                         <  1:2085101/150‑1 (MQ=255)
                               cTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGaaca                                                                                                                        >  1:4190327/1‑150 (MQ=255)
                                            gccgccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAg                                                                                                           <  2:2872344/150‑1 (MQ=255)
                                             ccgccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGt                                                                                                          <  1:3841906/150‑1 (MQ=255)
                                             ccgccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGt                                                                                                          <  2:3349848/150‑1 (MQ=255)
                                              cgccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGtt                                                                                                         >  1:784690/1‑150 (MQ=255)
                                              cgccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGtt                                                                                                         >  2:2362775/1‑150 (MQ=255)
                                              cgccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGtt                                                                                                         >  2:2208596/1‑150 (MQ=255)
                                               gccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGttt                                                                                                        >  1:3403578/1‑150 (MQ=255)
                                                ccAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATATCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTa                                                                                                       >  2:2140625/1‑150 (MQ=255)
                                                 cAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAg                                                                                                      >  2:942579/1‑150 (MQ=255)
                                                       gCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCgg                                                                                                <  2:3687113/150‑1 (MQ=255)
                                                         gTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTa                                                                                              >  1:2442889/1‑150 (MQ=255)
                                                          tGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTAt                                                                                             <  1:907552/150‑1 (MQ=255)
                                                          tGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTAt                                                                                             <  2:853912/150‑1 (MQ=255)
                                                           gATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTAtt                                                                                            <  1:3596239/150‑1 (MQ=255)
                                                            aTGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTg                                                                                           <  2:4131810/150‑1 (MQ=255)
                                                               cccTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCa                                                                                        <  2:3027905/150‑1 (MQ=255)
                                                                 cTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCAtt                                                                                      <  1:1760456/150‑1 (MQ=255)
                                                                 cTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCAtt                                                                                      <  1:1759753/150‑1 (MQ=255)
                                                                   cGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGt                                                                                    <  2:2544206/150‑1 (MQ=255)
                                                                   cGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGt                                                                                    <  2:3122353/150‑1 (MQ=255)
                                                                   cGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCAGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGt                                                                                    <  1:3484889/150‑1 (MQ=255)
                                                                    ggCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTc                                                                                   >  2:4356704/1‑150 (MQ=255)
                                                                     gCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCg                                                                                  <  2:2597737/150‑1 (MQ=255)
                                                                         aCGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAaga                                                                              <  1:1447413/150‑1 (MQ=255)
                                                                          cGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTAATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAagag                                                                             >  1:2504454/1‑150 (MQ=255)
                                                                           gACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGagc                                                                            >  2:2092041/1‑150 (MQ=255)
                                                                            aCTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGagca                                                                           >  2:422154/1‑150 (MQ=255)
                                                                                ccAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATATCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCtt                                                                       <  2:2491572/150‑1 (MQ=255)
                                                                                 cAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTc                                                                      <  2:2997794/150‑1 (MQ=255)
                                                                                    agTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGc                                                                   <  1:3766830/150‑1 (MQ=255)
                                                                                       cATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAAc                                                                >  1:2979968/1‑150 (MQ=255)
                                                                                        aTTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACt                                                               >  1:2563276/1‑150 (MQ=255)
                                                                                            ccAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGt                                                           >  1:3879677/1‑150 (MQ=255)
                                                                                             cAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTg                                                          >  2:2823209/1‑150 (MQ=255)
                                                                                                   tttatttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAgg                                                    <  1:1842879/150‑1 (MQ=255)
                                                                                                   tttatttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAgg                                                    <  1:4320831/150‑1 (MQ=255)
                                                                                                   tttatttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAgg                                                    <  1:4024533/150‑1 (MQ=255)
                                                                                                     tatttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATATCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGt                                                  >  2:2130212/1‑150 (MQ=255)
                                                                                                     tatttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATATCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGt                                                  >  1:3714926/1‑150 (MQ=255)
                                                                                                      atttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTc                                                 <  2:1479455/150‑1 (MQ=255)
                                                                                                      atttaCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTc                                                 >  1:2935646/1‑150 (MQ=255)
                                                                                                          aCATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGt                                             >  1:3124482/1‑150 (MQ=255)
                                                                                                           cATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGtg                                            >  1:2634760/1‑150 (MQ=255)
                                                                                                           cATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGtg                                            >  1:3144177/1‑150 (MQ=255)
                                                                                                             tAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGtgat                                          >  1:640009/1‑150 (MQ=255)
                                                                                                              agagTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGtgatg                                         <  2:4424688/150‑1 (MQ=255)
                                                                                                              agagTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATATCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGtgatg                                         <  1:2140625/150‑1 (MQ=255)
                                                                                                               gagTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGtgatga                                        >  2:147326/1‑150 (MQ=255)
                                                                                                                agTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGaa                                       >  1:2360202/1‑150 (MQ=255)
                                                                                                                agTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGaa                                       >  2:976641/1‑150 (MQ=255)
                                                                                                                 gTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAAt                                      <  2:3290595/150‑1 (MQ=255)
                                                                                                                 gTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAAt                                      >  2:776788/1‑150 (MQ=255)
                                                                                                                 gTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAAt                                      >  2:186678/1‑150 (MQ=255)
                                                                                                                  tCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATc                                     <  1:3828871/150‑1 (MQ=255)
                                                                                                                  tCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATc                                     <  2:3185058/150‑1 (MQ=255)
                                                                                                                   cAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCt                                    >  1:2957335/1‑150 (MQ=255)
                                                                                                                   cAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCt                                    >  1:677949/1‑150 (MQ=255)
                                                                                                                    aGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTg                                   >  1:1656056/1‑150 (MQ=255)
                                                                                                                    aGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTg                                   <  2:77058/150‑1 (MQ=255)
                                                                                                                     gTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGc                                  <  2:2660825/150‑1 (MQ=255)
                                                                                                                     gTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGc                                  >  2:3991773/1‑150 (MQ=255)
                                                                                                                       tACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGcaa                                >  2:4436451/1‑150 (MQ=255)
                                                                                                                        aCCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGcaac                               >  1:2177266/1‑150 (MQ=255)
                                                                                                                            gCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGa                           <  1:1717203/150‑1 (MQ=255)
                                                                                                                            gCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGa                           <  1:978744/150‑1 (MQ=255)
                                                                                                                               tCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACAt                        >  2:1151084/1‑150 (MQ=255)
                                                                                                                               tCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACAt                        <  1:2753827/150‑1 (MQ=255)
                                                                                                                                  cgACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTg                     >  2:2072156/1‑150 (MQ=255)
                                                                                                                                  cgACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTg                     >  2:3078114/1‑150 (MQ=255)
                                                                                                                                   gACCTCTTTCTGCGCTATATCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTgg                    <  2:1915274/150‑1 (MQ=255)
                                                                                                                                   caCCACTATCTGCGCTATCTCCGGACTCATGCTCTGGTCACGTCTGAACAACTGTCACATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGATTCAGCAACTGCGAGAAAAGTCTCTGGTGATGAATCTGCAACAAGACATcaat                    >  2:2204407/2‑147 (MQ=255)
                                                                                                                                       tctTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGa                <  1:1682765/150‑1 (MQ=255)
                                                                                                                                       tctTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGa                <  1:1077821/150‑1 (MQ=255)
                                                                                                                                       tctTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGa                >  1:58657/1‑150 (MQ=255)
                                                                                                                                        ctTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAg               >  1:2855272/1‑150 (MQ=255)
                                                                                                                                         tttCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCCTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGc              <  2:499718/150‑1 (MQ=255)
                                                                                                                                           tCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGa            <  2:4274373/150‑1 (MQ=255)
                                                                                                                                           tCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGa            <  2:608189/150‑1 (MQ=255)
                                                                                                                                            cTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGAt           <  1:2208596/150‑1 (MQ=255)
                                                                                                                                            cTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGAt           <  1:2362775/150‑1 (MQ=255)
                                                                                                                                                gcTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAg       >  1:3190023/1‑150 (MQ=255)
                                                                                                                                                 cTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAGt      <  2:240883/150‑1 (MQ=255)
                                                                                                                                                   aTCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAGTAt    >  1:3364678/1‑150 (MQ=255)
                                                                                                                                                    tctcCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAGTAtt   >  1:205256/1‑150 (MQ=255)
                                                                                                                                                     ctcCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAGTAttt  >  2:3709428/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCGATGATTAGCGGACAGGAGCGTCTTGCCTGTATGGATGATGCCGCCAGGCTGCGTGATGCCCTCGGCGTACGACTACCAGAGTCATTGCCAGAGATTTATTTACATAGAGTCAGTTACCCGCTTCGCGACCTCTTTCTGCGCTATCTCCGGGCTCATGCTCTGGTCACGGCTGAACAACTGGCTCATGAGTTTAGTCTCGGTATTGCCATTGTCGAAGAGCAGCTTCAGCAACTGCGTGAACAGGGTCTGGTGATGAATCTGCAACAAGACATCTGGGTGAGCGATGAAGTATTT  >  CP009273/1726288‑1726586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑