BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,801,813	A→T	8.1%	T53S (ACT→TCT)	ydiZ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,801,813	0	A	T	8.1%	899.6 / 55.3	283	T53S (ACT→TCT)	ydiZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (126/134); new base T (14/9); total (140/143)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 288 total aligned reads displayed.

GTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCA  >  CP009273/1801664‑1801961

gTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAaca                                                                                                                                                      >  1:1689464/1‑150 (MQ=255)

    cGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACttt                                                                                                                                                  >  2:1872945/1‑150 (MQ=255)

     gATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACtttt                                                                                                                                                 >  2:1150477/1‑150 (MQ=255)

       tCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTgg                                                                                                                                               >  2:2385644/1‑150 (MQ=255)

        cTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGa                                                                                                                                              <  1:2164891/150‑1 (MQ=255)

         ttGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGAc                                                                                                                                             >  1:3557618/1‑150 (MQ=255)

          tGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACt                                                                                                                                            <  2:2588210/150‑1 (MQ=255)

            tCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTa                                                                                                                                          <  1:3003678/150‑1 (MQ=255)

                ttATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAg                                                                                                                                      <  1:3742026/150‑1 (MQ=255)

                ttATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAg                                                                                                                                      >  2:2417525/1‑150 (MQ=255)

                ttATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCGGGGAACTAACACTTTTGGACTTATCAg                                                                                                                                      >  2:2754845/1‑150 (MQ=255)

                  aTGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTa                                                                                                                                    <  2:3189609/150‑1 (MQ=255)

                    gTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTAcc                                                                                                                                  <  1:2123241/150‑1 (MQ=255)

                     tCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTAccc                                                                                                                                 >  1:645861/1‑150 (MQ=255)

                       aTAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCAATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCaa                                                                                                                               >  1:239239/1‑150 (MQ=255)

                          aCCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGt                                                                                                                            <  1:1891993/150‑1 (MQ=255)

                             gTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaa                                                                                                                         <  2:4229904/150‑1 (MQ=255)

                             gTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaa                                                                                                                         <  2:445622/150‑1 (MQ=255)

                                aTGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaa                                                                                                                      >  2:3511949/1‑150 (MQ=255)

                                 tGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaag                                                                                                                     >  2:1530589/1‑150 (MQ=255)

                                 tGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaag                                                                                                                     >  1:2579478/1‑150 (MQ=255)

                                  gTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaaga                                                                                                                    >  2:2612300/1‑150 (MQ=255)

                                    tGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGaaa                                                                                                                  >  1:2912887/1‑150 (MQ=255)

                                      cATGATGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGTCTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGTGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAAtt                                                                                                                <  1:893459/150‑1 (MQ=255)

                                      cATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATTTCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAAtt                                                                                                                <  2:4015007/150‑1 (MQ=255)

                                      cATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAAtt                                                                                                                >  1:2533833/1‑150 (MQ=255)

                                       aTGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTa                                                                                                               <  1:235193/150‑1 (MQ=255)

                                       aTGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTa                                                                                                               <  2:3678936/150‑1 (MQ=255)

                                        tGAGGAGACATTGACTGAAATTAACGAGTTGAATACTTACCTGACTCGCGACGTTTTTTTGCTCACTATGACGGATGATGAGGGAAATATTCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTag                                                                                                              <  1:3220050/150‑1 (MQ=255)

                                         gAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTaga                                                                                                             >  2:3777297/1‑150 (MQ=255)

                                          aGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTagag                                                                                                            >  2:3739521/1‑150 (MQ=255)

                                          aGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTagag                                                                                                            <  1:94387/150‑1 (MQ=255)

                                              tACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACt                                                                                                        >  1:3503922/1‑150 (MQ=255)

                                                cATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTgg                                                                                                      >  1:621272/1‑150 (MQ=255)

                                                cATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTgg                                                                                                      <  2:645861/150‑1 (MQ=255)

                                                 aTTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGt                                                                                                     <  1:179990/150‑1 (MQ=255)

                                                  ttGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGtt                                                                                                    >  1:58710/1‑150 (MQ=255)

                                                   tGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGttt                                                                                                   >  1:862750/1‑150 (MQ=255)

                                                    gACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTc                                                                                                  >  1:2795862/1‑150 (MQ=255)

                                                       tGAAATTATCGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCggg                                                                                               >  1:3302066/1‑150 (MQ=255)

                                                       tGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCggg                                                                                               >  1:139584/1‑150 (MQ=255)

                                                       tGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCggg                                                                                               >  1:1181293/1‑150 (MQ=255)

                                                       tGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCggg                                                                                               <  2:1614898/150‑1 (MQ=255)

                                                       tGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCggg                                                                                               >  1:1326622/1‑150 (MQ=255)

                                                         aaaTTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGc                                                                                             >  2:1819668/1‑150 (MQ=255)

                                                           aTTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGGACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCtt                                                                                           <  2:2321660/150‑1 (MQ=255)

                                                            ttAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTa                                                                                          >  1:2710100/1‑150 (MQ=255)

                                                             tAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTAc                                                                                         >  1:1496723/1‑150 (MQ=255)

                                                                 taGTTTAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCaa                                                                                     <  1:778939/149‑1 (MQ=255)

                                                                  aGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCaaa                                                                                    >  1:1002367/1‑150 (MQ=255)

                                                                   gTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAg                                                                                   >  1:2242958/1‑150 (MQ=255)

                                                                   gTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAg                                                                                   >  1:2788072/1‑150 (MQ=255)

                                                                          aatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAAcc                                                                            >  2:2755577/1‑150 (MQ=255)

                                                                          aatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAAcc                                                                            >  2:1467720/1‑150 (MQ=255)

                                                                           atgaCCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCg                                                                           <  2:108332/147‑1 (MQ=255)

                                                                            ttACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCgg                                                                          <  2:3575041/150‑1 (MQ=255)

                                                                              aCCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCa                                                                        <  2:4064470/150‑1 (MQ=255)

                                                                              aCCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTAATCAGTACACAAAGAGAAGAAGAAATTAGATAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCa                                                                        >  1:3910543/1‑150 (MQ=255)

                                                                                 tGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAg                                                                     <  2:2081767/150‑1 (MQ=255)

                                                                                   aCTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGAt                                                                   <  1:3575742/150‑1 (MQ=255)

                                                                                    cTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATc                                                                  <  2:2143414/150‑1 (MQ=255)

                                                                                     tCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATcc                                                                 >  2:2677063/1‑150 (MQ=255)

                                                                                      cgcgACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCt                                                                <  2:4317449/150‑1 (MQ=255)

                                                                                        cgACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGa                                                              >  1:2079594/1‑150 (MQ=255)

                                                                                         gACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGaa                                                             >  2:22737/1‑150 (MQ=255)

                                                                                         gACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGaa                                                             >  1:2012800/1‑150 (MQ=255)

                                                                                          aCGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGaaa                                                            <  2:2467967/150‑1 (MQ=255)

                                                                                            ggTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATc                                                          <  1:1311298/150‑1 (MQ=255)

                                                                                            ggTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATc                                                          >  1:34521/1‑150 (MQ=255)

                                                                                            ggTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATc                                                          >  2:2845524/1‑150 (MQ=255)

                                                                                             gTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCa                                                         >  1:2763704/1‑150 (MQ=255)

                                                                                               tttttGCTCACTATGACGGATGATGTGGGAAATATACATTAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAATAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGATATCAcc                                                       <  2:3910543/150‑1 (MQ=255)

                                                                                               tttttGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCAcc                                                       <  2:881126/150‑1 (MQ=255)

                                                                                                  ttGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATc                                                    <  1:4296539/150‑1 (MQ=255)

                                                                                                   tGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCa                                                   <  1:4267630/150‑1 (MQ=255)

                                                                                                    gCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACTCTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCAc                                                  <  2:139584/150‑1 (MQ=255)

                                                                                                     cTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACg                                                 <  2:331927/150‑1 (MQ=255)

                                                                                                          tATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTg                                            <  2:3369375/150‑1 (MQ=255)

                                                                                                           aTGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTgg                                           <  2:1214768/150‑1 (MQ=255)

                                                                                                               cGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGggagg                                       >  1:1044148/1‑150 (MQ=255)

                                                                                                               cGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGggagg                                       >  2:25238/1‑150 (MQ=255)

                                                                                                                ggATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGggagga                                      >  2:2496212/1‑150 (MQ=255)

                                                                                                                  atgatgAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAggaat                                    <  2:1320679/150‑1 (MQ=255)

                                                                                                                     atgaGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAggaatgga                                 <  2:621253/150‑1 (MQ=255)

                                                                                                                       gaGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAggaatggaat                               >  2:3913751/1‑150 (MQ=255)

                                                                                                                        aGGTAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATa                              <  1:168229/150‑1 (MQ=255)

                                                                                                                          ggAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGc                            <  1:1771194/150‑1 (MQ=255)

                                                                                                                                atCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGa                      <  1:1110442/150‑1 (MQ=255)

                                                                                                                                atCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGa                      <  1:2385691/150‑1 (MQ=255)

                                                                                                                                atCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGa                      <  1:4429752/150‑1 (MQ=255)

                                                                                                                                 tCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGaa                     <  2:1717017/150‑1 (MQ=255)

                                                                                                                                    aTGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAaaat                  >  2:4352689/1‑150 (MQ=255)

                                                                                                                                     tGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAaaata                 >  1:4468237/1‑150 (MQ=255)

                                                                                                                                      gAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAaaataa                >  2:3078822/1‑150 (MQ=255)

                                                                                                                                       aGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAaaataaa               >  1:936893/1‑150 (MQ=255)

                                                                                                                                       aGCTGGGAACTAACACTTGTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAaaataaa               <  2:2986668/150‑1 (MQ=255)

                                                                                                                                              aaCTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGtttt        <  1:4239562/150‑1 (MQ=255)

                                                                                                                                                cTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGtttttt      >  2:3354461/1‑149 (MQ=255)

                                                                                                                                                  aaCACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTggg    <  1:4068319/150‑1 (MQ=255)

                                                                                                                                                    cacTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCa  >  1:805222/1‑150 (MQ=255)

GTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCA  >  CP009273/1801664‑1801961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`