BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,807,214	G→T	5.7%	A59D (GCC→GAC)	ydjO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,807,214	0	G	T	5.7%	1049.3 / 26.6	279	A59D (GCC→GAC)	ydjO	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (123/140); new base T (4/12); total (127/152)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 283 total aligned reads displayed.

AATCTTTATTTATACCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCACCATAATTA  >  CP009273/1807065‑1807363

       atttatACCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCat                                                                                                                                                >  2:2814874/1‑150 (MQ=255)

           ataCCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATaa                                                                                                                                            <  2:2638955/150‑1 (MQ=255)

              ccTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTa                                                                                                                                         <  2:2511812/150‑1 (MQ=255)

              ccTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTa                                                                                                                                         <  2:2512306/150‑1 (MQ=255)

                tctTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGTCAATCATATAATTaaa                                                                                                                                       <  1:3169326/150‑1 (MQ=255)

                 ctTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAg                                                                                                                                      <  1:2798998/150‑1 (MQ=255)

                   tATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGtt                                                                                                                                    >  1:3613002/1‑150 (MQ=255)

                   tATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGtt                                                                                                                                    >  1:1344878/1‑150 (MQ=255)

                   tATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGtt                                                                                                                                    <  1:1915489/150‑1 (MQ=255)

                    aTCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGttt                                                                                                                                   >  2:2176969/1‑150 (MQ=255)

                    aTCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGttt                                                                                                                                   >  1:548265/1‑150 (MQ=255)

                    aTCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGttt                                                                                                                                   >  2:4151479/1‑150 (MQ=255)

                           tttCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTg                                                                                                                            <  1:827147/150‑1 (MQ=255)

                                    cATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGTCAATCATATAATTAAAGTTTGATTCTGCTATTTCAt                                                                                                                   <  1:3944837/150‑1 (MQ=255)

                                     aTAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATc                                                                                                                  <  1:983311/150‑1 (MQ=255)

                                     aTAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATc                                                                                                                  <  2:683137/150‑1 (MQ=255)

                                          gATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGt                                                                                                             >  1:50827/1‑150 (MQ=255)

                                           attattaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTc                                                                                                            >  2:2853201/1‑150 (MQ=255)

                                           attattaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTc                                                                                                            >  2:191260/1‑150 (MQ=255)

                                           attattaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTc                                                                                                            >  2:191187/1‑150 (MQ=255)

                                               ttaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACAt                                                                                                        >  2:865037/1‑150 (MQ=255)

                                                 aattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTg                                                                                                      <  2:1521783/150‑1 (MQ=255)

                                                 aattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTg                                                                                                      >  1:3460804/1‑150 (MQ=255)

                                                 aattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTg                                                                                                      >  2:913295/1‑150 (MQ=255)

                                                 aattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTg                                                                                                      >  1:2895030/1‑150 (MQ=255)

                                                  attattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGa                                                                                                     >  2:4107423/1‑150 (MQ=255)

                                                      ttaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCg                                                                                                 >  2:1656599/1‑150 (MQ=255)

                                                      ttaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCg                                                                                                 >  2:1656584/1‑150 (MQ=255)

                                                       taCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGt                                                                                                <  2:460973/150‑1 (MQ=255)

                                                       taCTCTAAATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGt                                                                                                <  2:3538050/150‑1 (MQ=255)

                                                        aCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTTGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGtt                                                                                               <  2:3296720/150‑1 (MQ=255)

                                                         ctctATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTg                                                                                              <  1:1662033/150‑1 (MQ=255)

                                                         ctctATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTg                                                                                              >  1:2365542/1‑150 (MQ=255)

                                                            tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGTCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGata                                                                                           <  1:3021713/150‑1 (MQ=255)

                                                            tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGata                                                                                           >  1:1184931/1‑150 (MQ=255)

                                                            tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGata                                                                                           <  2:487098/150‑1 (MQ=255)

                                                            tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGata                                                                                           <  2:2730332/150‑1 (MQ=255)

                                                            tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGata                                                                                           <  1:2488493/150‑1 (MQ=255)

                                                             atatTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGTCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGatat                                                                                          >  2:1670983/1‑150 (MQ=255)

                                                             atatTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGatat                                                                                          >  2:2278983/1‑150 (MQ=255)

                                                             atatTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATACTGCTATTTCATCGCAGTCACATTGACCCGTTGatat                                                                                          >  1:4019277/1‑150 (MQ=255)

                                                               atTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaa                                                                                        >  2:1583760/1‑150 (MQ=255)

                                                                tttAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaa                                                                                       <  2:620353/150‑1 (MQ=255)

                                                                 ttAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaag                                                                                      <  1:4533552/150‑1 (MQ=255)

                                                                   aaCATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaagaa                                                                                    >  2:2099905/1‑150 (MQ=255)

                                                                    aCATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaagaaa                                                                                   <  1:2543625/150‑1 (MQ=255)

                                                                      aTTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagt                                                                                 >  1:2628530/1‑150 (MQ=255)

                                                                        tGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtga                                                                               <  1:135658/150‑1 (MQ=255)

                                                                            cTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGc                                                                           <  1:4343616/150‑1 (MQ=255)

                                                                            cTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGc                                                                           <  1:3893990/150‑1 (MQ=255)

                                                                            cTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGc                                                                           <  1:2096452/150‑1 (MQ=255)

                                                                            cTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGc                                                                           <  2:761877/150‑1 (MQ=255)

                                                                               cAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGt                                                                        >  1:1306041/1‑150 (MQ=255)

                                                                                  atatGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAACAAAGTGAGTGCTGTCAt                                                                     >  2:1806136/1‑150 (MQ=255)

                                                                                    atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCAtag                                                                   <  1:1466183/150‑1 (MQ=255)

                                                                                    atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCAtag                                                                   >  2:3116007/1‑150 (MQ=255)

                                                                                    atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCAtag                                                                   >  1:2861522/1‑150 (MQ=255)

                                                                                     tGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCAtagt                                                                  <  1:3362073/150‑1 (MQ=255)

                                                                                      gTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCAtagta                                                                 >  2:3574174/1‑150 (MQ=255)

                                                                                        aTCAAAACTAAGTGCTGGCGCACCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAATTTTGATTCTGCTATTTCATCGCAGTCACATTGACACGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGa                                                               >  1:2811330/1‑150 (MQ=255)

                                                                                         tCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGAc                                                              <  2:3460449/150‑1 (MQ=255)

                                                                                          cAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACt                                                             <  2:4061571/150‑1 (MQ=255)

                                                                                          cAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACt                                                             <  2:4062300/150‑1 (MQ=255)

                                                                                           aaaaCTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGACAGTGAGTGCTGTCATAGTAGACTg                                                            >  2:4020317/1‑150 (MQ=255)

                                                                                            aaaCTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGc                                                           >  2:3070529/1‑150 (MQ=255)

                                                                                              aCTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAg                                                         >  2:3960730/1‑150 (MQ=255)

                                                                                                tAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGcc                                                       >  2:3653534/1‑150 (MQ=255)

                                                                                                 aaGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGATGATAAAAAGAAAGTGATTGCTGTCATAGTAGACTGCAGCct                                                      >  1:3260896/1‑150 (MQ=255)

                                                                                                  aGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCctt                                                     <  2:2380541/150‑1 (MQ=255)

                                                                                                    tGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCctttc                                                   <  2:1724612/150‑1 (MQ=255)

                                                                                                    tGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCctttc                                                   <  2:1756811/150‑1 (MQ=255)

                                                                                                       tGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGTCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCctttcttt                                                <  1:1670983/150‑1 (MQ=255)

                                                                                                       tGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCctttcttt                                                <  1:3839615/150‑1 (MQ=255)

                                                                                                       tGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCctttcttt                                                >  1:3792716/1‑150 (MQ=255)

                                                                                                               cTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGTCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGt                                        <  1:1112291/150‑1 (MQ=255)

                                                                                                               cTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGt                                        <  1:790333/150‑1 (MQ=255)

                                                                                                               cTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGt                                        <  1:2202085/150‑1 (MQ=255)

                                                                                                                tGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTc                                       <  2:3170041/150‑1 (MQ=255)

                                                                                                                tGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTc                                       <  2:3511791/150‑1 (MQ=255)

                                                                                                                       gAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGcc                                >  1:2283328/1‑150 (MQ=255)

                                                                                                                        aGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCa                               <  1:1265745/150‑1 (MQ=255)

                                                                                                                        aGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCa                               <  1:1264520/150‑1 (MQ=255)

                                                                                                                           catcatAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGc                            >  2:4160245/1‑150 (MQ=255)

                                                                                                                            atcatAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCt                           <  2:3096148/150‑1 (MQ=255)

                                                                                                                                tAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTa                       <  2:3424048/150‑1 (MQ=255)

                                                                                                                                tAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTa                       <  1:3508589/150‑1 (MQ=255)

                                                                                                                                tAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTa                       <  2:1151350/150‑1 (MQ=255)

                                                                                                                                   ttAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAata                    <  2:2152065/150‑1 (MQ=255)

                                                                                                                                    tAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAatat                   <  2:2597048/150‑1 (MQ=255)

                                                                                                                                      aTGGTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCAACGAAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGACATAGTAGACTTCAGCCTTTCTTTAAAAGAGACGAAATCCAGGCTATTAATAttt                 >  2:1289249/1‑150 (MQ=255)

                                                                                                                                        gTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTaa               <  1:3469582/150‑1 (MQ=255)

                                                                                                                                         ttAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTaaa              >  1:3035107/1‑150 (MQ=255)

                                                                                                                                          tAGACATATAGTAAATCATATAATTTAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAAt             <  2:3906279/150‑1 (MQ=255)

                                                                                                                                            gACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCa           >  2:3794208/1‑150 (MQ=255)

                                                                                                                                              cATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCTCATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCAcc         <  1:687148/150‑1 (MQ=255)

                                                                                                                                              cATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCAcc         >  2:298050/1‑150 (MQ=255)

                                                                                                                                                 ataGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCACCATa      <  1:4185284/150‑1 (MQ=255)

                                                                                                                                                 ataGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCATTTAATATTTAAATCACCATa      >  1:4418547/1‑150 (MQ=255)

                                                                                                                                                   aGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCACCATAAt    <  1:3417871/150‑1 (MQ=255)

                                                                                                                                                     gCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCACCATAATTa  <  1:261116/150‑1 (MQ=255)

AATCTTTATTTATACCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTAATATTTAAATCACCATAATTA  >  CP009273/1807065‑1807363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`