Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,899,495 G→A 6.0% K146K (AAG→AAA)  yobD → UPF0266 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,899,4950GA 6.0% 975.5 / 35.0 281K146K (AAG→AAA) yobDUPF0266 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (137/127);  new base A (7/10);  total (144/137)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 285 total aligned reads displayed.

AAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAA  >  CP009273/1899346‑1899642
                                                                                                                                                     |                                                                                                                                                   
 aaGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGc                                                                                                                                                    >  1:3094040/1‑150 (MQ=255)
   ggTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCtt                                                                                                                                                  >  2:3297133/1‑150 (MQ=255)
   ggTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCtt                                                                                                                                                  <  1:3163090/150‑1 (MQ=255)
    gTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAACTtc                                                                                                                                                 <  2:1514997/150‑1 (MQ=255)
      tttttCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTtctc                                                                                                                                               >  2:929960/1‑150 (MQ=255)
           cttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTc                                                                                                                                          >  2:3053693/1‑150 (MQ=255)
           cttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTc                                                                                                                                          <  2:1646359/150‑1 (MQ=255)
           cttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTc                                                                                                                                          >  1:3831918/1‑150 (MQ=255)
           cttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTc                                                                                                                                          >  1:2605806/1‑150 (MQ=255)
              cGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAAc                                                                                                                                       <  1:4251597/150‑1 (MQ=255)
               gCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACt                                                                                                                                      <  2:1751419/150‑1 (MQ=255)
                        tGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGtt                                                                                                                             <  1:1281280/150‑1 (MQ=255)
                            ttGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGa                                                                                                                         <  1:3850158/150‑1 (MQ=255)
                             tGAATATAGCCGAATCAAAGCGATGAGCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGaa                                                                                                                        <  2:2066842/150‑1 (MQ=255)
                              gAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAACTTCTCGTTTCAACTCAATAAGTTATGAAt                                                                                                                       >  2:3989318/1‑150 (MQ=255)
                               aaTATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAtt                                                                                                                      <  1:1246919/150‑1 (MQ=255)
                                atatAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAttt                                                                                                                     >  2:651248/1‑150 (MQ=255)
                                atatAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAttt                                                                                                                     >  2:146396/1‑150 (MQ=255)
                                atatAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAttt                                                                                                                     <  2:4291041/150‑1 (MQ=255)
                                atatAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAttt                                                                                                                     <  2:1580107/150‑1 (MQ=255)
                                   taGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGc                                                                                                                  >  2:3062463/1‑150 (MQ=255)
                                   taGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGc                                                                                                                  >  2:3062732/1‑150 (MQ=255)
                                     gCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCa                                                                                                                <  1:1276613/150‑1 (MQ=255)
                                       cGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCaaa                                                                                                              >  1:3644027/1‑150 (MQ=255)
                                         aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCaaaac                                                                                                            >  2:396052/1‑148 (MQ=255)
                                         aaTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGc                                                                                                            <  1:4379933/150‑1 (MQ=255)
                                          aTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAATTCAATAAGTTATGAATTTAGCCAAAGCt                                                                                                           <  1:1383234/150‑1 (MQ=255)
                                          aTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAATTCAATAAGTTATGAATTTAGCCAAAGCt                                                                                                           <  1:1383113/150‑1 (MQ=255)
                                          aTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCt                                                                                                           >  2:776537/1‑150 (MQ=255)
                                          aTCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCt                                                                                                           >  2:2037070/1‑150 (MQ=255)
                                           tCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTa                                                                                                          <  1:1092818/150‑1 (MQ=255)
                                                 cGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTa                                                                                                    <  2:720720/150‑1 (MQ=255)
                                                   aTGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAATTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgt                                                                                                  <  1:2825319/150‑1 (MQ=255)
                                                   aTGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgt                                                                                                  <  1:2824769/150‑1 (MQ=255)
                                                    tGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtg                                                                                                 >  2:3337777/1‑150 (MQ=255)
                                                     gAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtgt                                                                                                >  2:2261152/1‑150 (MQ=255)
                                                      aaCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTa                                                                                               <  2:2518446/150‑1 (MQ=255)
                                                           gTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTAttttt                                                                                          >  2:1449810/1‑150 (MQ=255)
                                                           gTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTAttttt                                                                                          >  2:3930713/1‑150 (MQ=255)
                                                              ggAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaat                                                                                       <  1:373416/150‑1 (MQ=255)
                                                              ggAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaat                                                                                       >  1:1009802/1‑150 (MQ=255)
                                                              ggAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaat                                                                                       >  1:1873546/1‑150 (MQ=255)
                                                               gAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaata                                                                                      >  2:3681614/1‑150 (MQ=255)
                                                                       cGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                              >  2:2006600/1‑150 (MQ=255)
                                                                       cGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                              >  2:2006281/1‑150 (MQ=255)
                                                                        gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACAt                                                                             <  1:2216279/150‑1 (MQ=255)
                                                                        gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACAt                                                                             <  1:2428068/150‑1 (MQ=255)
                                                                        gtgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACAt                                                                             <  2:3174315/150‑1 (MQ=255)
                                                                          gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                           >  2:2818325/1‑150 (MQ=255)
                                                                          gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                           >  2:293928/1‑150 (MQ=255)
                                                                          gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAg                                                                           <  1:713844/150‑1 (MQ=255)
                                                                           ttGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGc                                                                          >  2:1448093/1‑150 (MQ=255)
                                                                           ttGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGc                                                                          >  2:4184599/1‑150 (MQ=255)
                                                                           ttGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGc                                                                          >  2:1282594/1‑150 (MQ=255)
                                                                             ggTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCtt                                                                        <  1:3741106/150‑1 (MQ=255)
                                                                             ggTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCtt                                                                        >  2:2997044/1‑150 (MQ=255)
                                                                             ggTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCtt                                                                        >  2:1969835/1‑150 (MQ=255)
                                                                                 aTGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGc                                                                    <  1:3567614/150‑1 (MQ=255)
                                                                                 aTGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGc                                                                    <  1:2885834/150‑1 (MQ=255)
                                                                                     aaTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtata                                                                <  2:2467316/150‑1 (MQ=255)
                                                                                      aTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTatat                                                               <  1:1972093/150‑1 (MQ=255)
                                                                                       ttAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATAtt                                                              <  2:413681/150‑1 (MQ=255)
                                                                                           aaCAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACct                                                          <  1:494056/150‑1 (MQ=255)
                                                                                              aGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCtt                                                       <  1:1445329/150‑1 (MQ=255)
                                                                                                 gTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTccc                                                    >  1:3985502/1‑150 (MQ=255)
                                                                                                  tCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCt                                                   <  2:3475445/150‑1 (MQ=255)
                                                                                                   cGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAATTTATGAATTTAGCCAAAGCTATGGTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCtt                                                  >  1:2698575/1‑150 (MQ=255)
                                                                                                      cTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                               <  1:4409459/150‑1 (MQ=255)
                                                                                                      cTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                               >  2:4324432/1‑150 (MQ=255)
                                                                                                          tAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGtt                                           <  1:993666/150‑1 (MQ=255)
                                                                                                          tAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAACTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGtt                                           >  1:4500762/1‑150 (MQ=255)
                                                                                                           aaTCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGtta                                          >  2:529881/1‑150 (MQ=255)
                                                                                                           aaTCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGtta                                          >  2:1381140/1‑150 (MQ=255)
                                                                                                                gcgTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttatttat                                     >  2:4168382/1‑150 (MQ=255)
                                                                                                                gcgTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttatttat                                     <  1:204268/150‑1 (MQ=255)
                                                                                                                  gTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTatttattt                                   >  1:3897874/1‑150 (MQ=255)
                                                                                                                   ttCGAAATATCGACGATCTGGAAAAAATTTATAAACTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTAtttt                                  <  1:102987/150‑1 (MQ=255)
                                                                                                                      gAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                               >  2:26394/1‑150 (MQ=255)
                                                                                                                      gAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                               >  2:1268665/1‑150 (MQ=255)
                                                                                                                      gAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAAc                               >  2:1268537/1‑150 (MQ=255)
                                                                                                                              gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                       <  2:3661893/150‑1 (MQ=255)
                                                                                                                                 gaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGat                    >  1:1430148/1‑150 (MQ=255)
                                                                                                                                  aTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGata                   >  2:2140222/1‑150 (MQ=255)
                                                                                                                                    cttgAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatata                 <  2:3824085/147‑1 (MQ=255)
                                                                                                                                      gtaaaaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGAtatata               <  2:511491/148‑1 (MQ=255)
                                                                                                                                      ggAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGAtatata               >  1:1773035/1‑150 (MQ=255)
                                                                                                                                      ggAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGAtatata               >  1:1759784/1‑150 (MQ=255)
                                                                                                                                       gAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaa              >  1:2402988/1‑150 (MQ=255)
                                                                                                                                       gAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTGATTTATTTTAACGTTTCATTGATCTATaa              >  2:4113828/1‑150 (MQ=255)
                                                                                                                                       gAAAAAATTTATAAGCTTCTCGTTCCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaa              >  2:4529414/1‑150 (MQ=255)
                                                                                                                                         aaaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAAt            >  1:416615/1‑150 (MQ=255)
                                                                                                                                          aaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATc           >  1:939764/1‑150 (MQ=255)
                                                                                                                                           aaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATcc          >  1:3439085/1‑150 (MQ=255)
                                                                                                                                             aTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCaa        <  1:1687921/150‑1 (MQ=255)
                                                                                                                                             aTTTATAAACTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTGTTTATTTTAACGTTTCATTGATATATAAATCCaa        >  2:878141/1‑150 (MQ=255)
                                                                                                                                                tataAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATg     >  2:1848483/1‑150 (MQ=255)
                                                                                                                                                 ataAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGATTAGGCTATATTACATCTTCCCTAATTTGTTATTTATTTTAACGTTTCAGTGATATATAAATCCAAATGa    >  1:1542223/1‑150 (MQ=255)
                                                                                                                                                 ataAGCTTCTCGTTTCAACTCAATAAATTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGa    <  2:1367284/150‑1 (MQ=255)
                                                                                                                                                   aaGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGaaa  <  2:3681862/150‑1 (MQ=255)
                                                                                                                                                   aaGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGaaa  >  1:1925972/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAA  >  CP009273/1899346‑1899642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑