Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,905,599 C→A 10.6% A356E (GCG→GAG)  yebQ → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,905,5990CA10.6% 945.2 / 60.9 272A356E (GCG→GAG) yebQputative transporter
Reads supporting (aligned to +/- strand):  ref base C (106/137);  new base A (8/21);  total (114/158)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 273 total aligned reads displayed.

AGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACGGCT  >  CP009273/1905450‑1905744
                                                                                                                                                     |                                                                                                                                                 
aGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCcgc                                                                                                                                                   >  2:349777/1‑150 (MQ=255)
   tttACTTCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTc                                                                                                                                                >  1:2849727/1‑150 (MQ=255)
        ttCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTg                                                                                                                                           <  2:1815400/150‑1 (MQ=255)
           tGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCgg                                                                                                                                        >  1:2677888/1‑150 (MQ=255)
           tGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCGTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCgg                                                                                                                                        >  1:2678004/1‑150 (MQ=255)
             acacCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGat                                                                                                                                      <  1:1988000/150‑1 (MQ=255)
             acacCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGat                                                                                                                                      <  1:795064/150‑1 (MQ=255)
              cacCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGata                                                                                                                                     >  2:197147/1‑150 (MQ=255)
                   tGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAat                                                                                                                                >  2:3063509/1‑150 (MQ=255)
                   tGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAat                                                                                                                                >  1:3363450/1‑150 (MQ=255)
                    ggCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAata                                                                                                                               >  1:1174489/1‑150 (MQ=255)
                    ggCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAata                                                                                                                               >  1:3170888/1‑150 (MQ=255)
                      ccGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAAtatt                                                                                                                             <  1:2573628/150‑1 (MQ=255)
                      ccGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCGTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAatata                                                                                                                             >  1:3609692/1‑149 (MQ=255)
                        gTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAAtattat                                                                                                                           >  2:189827/1‑150 (MQ=255)
                         ttAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATc                                                                                                                          <  2:4123688/150‑1 (MQ=255)
                             cAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGc                                                                                                                      <  1:2243154/150‑1 (MQ=255)
                              aaCGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGcc                                                                                                                     >  2:2587011/1‑150 (MQ=255)
                              aaCGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGcc                                                                                                                     <  1:4279251/150‑1 (MQ=255)
                                  aTGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCgatg                                                                                                                 <  1:574469/150‑1 (MQ=255)
                                      tGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCt                                                                                                             <  2:73032/150‑1 (MQ=255)
                                      tGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAACATTATCTGGCCGATGATCt                                                                                                             >  1:3144181/1‑150 (MQ=255)
                                      tGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCt                                                                                                             <  1:2933152/150‑1 (MQ=255)
                                      tGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCt                                                                                                             <  1:651006/150‑1 (MQ=255)
                                       gATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCtt                                                                                                            >  2:2678610/1‑150 (MQ=255)
                                       gATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCtt                                                                                                            <  2:4326368/150‑1 (MQ=255)
                                         tGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAt                                                                                                          <  1:2813554/150‑1 (MQ=255)
                                         tGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAt                                                                                                          <  1:3104420/150‑1 (MQ=255)
                                         tGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAt                                                                                                          <  1:3118638/150‑1 (MQ=255)
                                          ggCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAtg                                                                                                         <  1:2719523/150‑1 (MQ=255)
                                          ggCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAtg                                                                                                         <  1:431426/150‑1 (MQ=255)
                                          ggCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAtg                                                                                                         <  1:3321646/150‑1 (MQ=255)
                                          ggCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAtg                                                                                                         <  1:1416101/150‑1 (MQ=255)
                                           gCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAtgt                                                                                                        <  2:33583/150‑1 (MQ=255)
                                            cTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTAtgtg                                                                                                       <  2:4305576/150‑1 (MQ=255)
                                              ccGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATgtggt                                                                                                     >  1:568013/1‑150 (MQ=255)
                                                gCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGc                                                                                                   <  2:1538240/150‑1 (MQ=255)
                                                   ggCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTgg                                                                                                <  1:442452/150‑1 (MQ=255)
                                                   ggCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTgg                                                                                                >  1:4249934/1‑150 (MQ=255)
                                                      aGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGAtt                                                                                             >  2:1359186/1‑150 (MQ=255)
                                                      aGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGAtt                                                                                             >  2:3626061/1‑150 (MQ=255)
                                                        gCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTg                                                                                           <  1:2408081/150‑1 (MQ=255)
                                                        gCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTg                                                                                           <  1:2326262/150‑1 (MQ=255)
                                                          tATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGc                                                                                         <  1:1158111/150‑1 (MQ=255)
                                                               gattgaACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTAtt                                                                                    <  1:337973/150‑1 (MQ=255)
                                                                attgaACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTc                                                                                   >  1:3266453/1‑150 (MQ=255)
                                                                attgaACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATAATATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTc                                                                                   <  1:3701264/150‑1 (MQ=255)
                                                                  tgaACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCa                                                                                 <  1:2627365/150‑1 (MQ=255)
                                                                   gaACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAg                                                                                <  2:399901/150‑1 (MQ=255)
                                                                     aCGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGTCTTTTTTCCCTGGTTCTGCTGCCAGCGTCACCTGCGGATATCAATTTTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTAGTCCAGTc                                                                              <  2:3311848/150‑1 (MQ=255)
                                                                      cgcgTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCa                                                                             >  1:1088949/1‑150 (MQ=255)
                                                                         gTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCAccc                                                                          <  1:1011438/150‑1 (MQ=255)
                                                                            cATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAAt                                                                       >  2:3055555/1‑150 (MQ=255)
                                                                            cATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCC‑GTCACCCAATa                                                                      <  2:1073417/150‑1 (MQ=255)
                                                                              tGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATaa                                                                     <  1:2333024/150‑1 (MQ=255)
                                                                              tGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATaa                                                                     <  1:2333222/150‑1 (MQ=255)
                                                                               gCAGGAGTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAAc                                                                    <  2:4058802/150‑1 (MQ=255)
                                                                                  ggATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACcac                                                                 <  2:3803422/150‑1 (MQ=255)
                                                                                  ggATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACcac                                                                 >  2:2523724/1‑150 (MQ=255)
                                                                                   gATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACcaca                                                                <  2:134410/150‑1 (MQ=255)
                                                                                     ttGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACAcc                                                              <  1:410442/150‑1 (MQ=255)
                                                                                      tGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCa                                                             >  2:1171025/1‑150 (MQ=255)
                                                                                         tGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCatta                                                          <  2:2757839/150‑1 (MQ=255)
                                                                                          gggggCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCattat                                                         >  2:3693554/1‑150 (MQ=255)
                                                                                              gCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTAGTCCAGTCACCCAATAACCACACCATTATTAcc                                                     <  2:3907315/150‑1 (MQ=255)
                                                                                               cTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCt                                                    <  2:2802671/150‑1 (MQ=255)
                                                                                                 ttAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTcc                                                  <  2:1333663/150‑1 (MQ=255)
                                                                                                    gggTTGTTCATCATGGCTGCGGGGCTTTTGTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCcgcg                                               <  2:1433078/150‑1 (MQ=255)
                                                                                                    gggTTGTTCATAATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATATTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCAAACCATAATTACCTCcgcc                                               >  2:763661/1‑149 (MQ=255)
                                                                                                     cgttgttCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCgcgc                                              >  2:1284705/2‑150 (MQ=255)
                                                                                                       ttgttCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCt                                            <  2:1839679/150‑1 (MQ=255)
                                                                                                       ttgttCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCt                                            <  2:578127/150‑1 (MQ=255)
                                                                                                        tgttCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTc                                           <  2:2449589/150‑1 (MQ=255)
                                                                                                          ttCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTcgc                                         >  2:3353929/1‑150 (MQ=255)
                                                                                                          ttCATCATGGATGCGGGGCTTTTTTCCCTGGTTCTGATGCCCGCGACACCTGCGGATATCAAAATTATCTTGCCGATGATCTTATGTGGTGCTGGATTTGGATTATAACAGTCACACAAAAACCACACCAATATTACCTCCGCGCCTcgc                                         >  2:1077766/1‑150 (MQ=255)
                                                                                                           tcatcaTGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTcgcg                                        <  2:2096735/150‑1 (MQ=255)
                                                                                                              tcatGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAAc                                     <  1:1531810/150‑1 (MQ=255)
                                                                                                              tcatGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAAc                                     <  1:2175955/150‑1 (MQ=255)
                                                                                                                  ggCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAg                                 <  2:3226204/150‑1 (MQ=255)
                                                                                                                   gCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGc                                <  2:1227069/150‑1 (MQ=255)
                                                                                                                    cTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCg                               <  2:2244901/150‑1 (MQ=255)
                                                                                                                    cTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCg                               <  1:3450263/150‑1 (MQ=255)
                                                                                                                     tGCGGGGCTTTTTTCCCTGGTTCTGCCGCCCGCGTCACCTGCTGATATCAATATTATCTGGCCGATGATCTTATGAGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCgg                              >  2:2388701/1‑150 (MQ=255)
                                                                                                                      gCGGGGCGTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGt                             <  2:4415387/150‑1 (MQ=255)
                                                                                                                        ggggCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTgg                           >  1:1295970/1‑150 (MQ=255)
                                                                                                                        ggggCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTgg                           >  2:1576790/1‑150 (MQ=255)
                                                                                                                         gggCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGa                          <  2:2545504/150‑1 (MQ=255)
                                                                                                                            cTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGcc                       <  1:2786775/150‑1 (MQ=255)
                                                                                                                              tttttCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAg                     >  2:3406520/1‑150 (MQ=255)
                                                                                                                                    ccTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCAt               >  2:4186477/1‑150 (MQ=255)
                                                                                                                                      tGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGt             >  2:2946165/1‑150 (MQ=255)
                                                                                                                                       ggTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGtt            <  1:3047032/150‑1 (MQ=255)
                                                                                                                                       ggTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGtt            <  1:3727885/150‑1 (MQ=255)
                                                                                                                                            tgctgcCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGaa       >  1:172612/1‑150 (MQ=255)
                                                                                                                                              ctgcCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACg     >  1:4321716/1‑150 (MQ=255)
                                                                                                                                              ctgcCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACg     <  2:2677888/150‑1 (MQ=255)
                                                                                                                                               tgcCCGAGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACgg    >  1:994130/1‑150 (MQ=255)
                                                                                                                                               tgcCCGAGTCACCTGCGGAGATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACgg    >  1:1161943/1‑150 (MQ=255)
                                                                                                                                                gcCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACGGc   >  1:3284778/1‑150 (MQ=255)
                                                                                                                                                 cccGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACGGCt  >  2:2544080/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTTTTTCCCTGGTTCTGCTGCCCGCGTCACCTGCGGATATCAATATTATCTGGCCGATGATCTTATGTGGTGCTGGATTTGGCTTATTCCAGTCACCCAATAACCACACCATTATTACCTCCGCGCCTCGCGAACGTAGCGGTGGAGCCAGTGGCATGTTAGGAACGGCT  >  CP009273/1905450‑1905744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑