BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,932,701	C→A	5.9%	Q266K (CAG→AAG)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,932,701	0	C	A	5.9%	956.7 / 23.8	252	Q266K (CAG→AAG)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand): ref base C (117/120); new base A (6/9); total (123/129)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 253 total aligned reads displayed.

TGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCTGTG  >  CP009273/1932553‑1932850

     cGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAg                                                                                                                                                 <  1:1008935/150‑1 (MQ=255)

     cGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAg                                                                                                                                                 <  1:1173365/150‑1 (MQ=255)

     cGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAg                                                                                                                                                 <  2:1106250/150‑1 (MQ=255)

          aTTGTTGCCTAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTAGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTg                                                                                                                                            <  1:3702607/150‑1 (MQ=255)

          aTTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTg                                                                                                                                            <  1:2062222/150‑1 (MQ=255)

            tgttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGAt                                                                                                                                          >  2:3331987/1‑150 (MQ=255)

                gCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCcgt                                                                                                                                      <  2:744427/150‑1 (MQ=255)

                     ggTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgc                                                                                                                                 >  2:844882/1‑150 (MQ=255)

                     ggTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgc                                                                                                                                 >  2:3857820/1‑150 (MQ=255)

                       ttGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTgcgc                                                                                                                               >  2:174272/1‑150 (MQ=255)

                        tGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGcgcg                                                                                                                              <  1:4295800/150‑1 (MQ=255)

                          aaCGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTc                                                                                                                            >  1:2504572/1‑150 (MQ=255)

                           aCGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCa                                                                                                                           >  1:2782056/1‑150 (MQ=255)

                             gTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGc                                                                                                                         >  1:4077911/1‑150 (MQ=255)

                             gTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGc                                                                                                                         >  2:3138980/1‑150 (MQ=255)

                               gCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGAGTTGATCCGTCGTGCGCGTCAGCTa                                                                                                                       >  1:679624/1‑150 (MQ=255)

                                cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTaa                                                                                                                      <  1:1570668/150‑1 (MQ=255)

                                cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTaa                                                                                                                      <  1:3830451/150‑1 (MQ=255)

                                 ggAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTaaa                                                                                                                     <  1:129254/150‑1 (MQ=255)

                                  gAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAAc                                                                                                                    >  1:2390063/1‑150 (MQ=255)

                                  gAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAAc                                                                                                                    >  1:3334902/1‑150 (MQ=255)

                                     gCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGa                                                                                                                 <  1:1398839/150‑1 (MQ=255)

                                               gCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCa                                                                                                       <  1:1998334/150‑1 (MQ=255)

                                                 cAGGATGAAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                                                     >  2:4188874/1‑150 (MQ=255)

                                                  aGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                                                                                    >  2:4088764/1‑150 (MQ=255)

                                                   ggATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGc                                                                                                   >  1:3355143/1‑150 (MQ=255)

                                                   ggATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGc                                                                                                   <  2:2322797/150‑1 (MQ=255)

                                                    gATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                                                  >  2:3113953/1‑150 (MQ=255)

                                                      tGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                                                                                >  1:579612/1‑150 (MQ=255)

                                                      tGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCTTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                                                                                <  1:3252626/150‑1 (MQ=255)

                                                        cAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                                                              <  1:4131118/150‑1 (MQ=255)

                                                         aaTGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCa                                                                                             >  2:2053429/1‑150 (MQ=255)

                                                           tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAga                                                                                           >  2:2481598/1‑150 (MQ=255)

                                                           tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAga                                                                                           >  2:4243788/1‑150 (MQ=255)

                                                             gATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatg                                                                                         <  2:857239/150‑1 (MQ=255)

                                                              aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatga                                                                                        <  1:2833710/150‑1 (MQ=255)

                                                               tGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatgat                                                                                       <  1:2465682/150‑1 (MQ=255)

                                                                   atcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                                                                                   <  1:3182461/150‑1 (MQ=255)

                                                                    tcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGt                                                                                  >  2:2105494/1‑150 (MQ=255)

                                                                     catcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTc                                                                                 >  1:3387874/1‑150 (MQ=255)

                                                                      atcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCa                                                                                >  1:2136058/1‑150 (MQ=255)

                                                                           cGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAGTGAt                                                                           >  1:2571436/1‑150 (MQ=255)

                                                                              ctctGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                                                        <  1:2874798/150‑1 (MQ=255)

                                                                               tctGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACt                                                                       <  2:3227001/150‑1 (MQ=255)

                                                                                ctGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTa                                                                      <  2:1150315/150‑1 (MQ=255)

                                                                                ctGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTa                                                                      >  1:2175884/1‑150 (MQ=255)

                                                                                  gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAc                                                                    >  1:4250543/1‑150 (MQ=255)

                                                                                   aCGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcc                                                                   >  1:4367587/1‑150 (MQ=255)

                                                                                    cGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAccc                                                                  >  1:3960298/1‑150 (MQ=255)

                                                                                     gTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCg                                                                 >  1:1892288/1‑150 (MQ=255)

                                                                                     gTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCg                                                                 >  1:88081/1‑150 (MQ=255)

                                                                                      tGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGAGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGa                                                                >  2:3513230/1‑150 (MQ=255)

                                                                                       ggTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGAt                                                               <  1:3123079/150‑1 (MQ=255)

                                                                                       ggTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGAt                                                               <  1:3123184/150‑1 (MQ=255)

                                                                                        gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                                                              <  2:158909/150‑1 (MQ=255)

                                                                                           aTGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCg                                                           >  1:1109820/1‑150 (MQ=255)

                                                                                              ggtGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAcg                                                        <  2:2157765/148‑1 (MQ=255)

                                                                                                 gCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGt                                                     <  2:1063629/150‑1 (MQ=255)

                                                                                                    cGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCa                                                  <  1:1199140/150‑1 (MQ=255)

                                                                                                     gTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTAAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAg                                                 <  1:2353940/150‑1 (MQ=255)

                                                                                                         cGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGt                                             <  2:4478296/150‑1 (MQ=255)

                                                                                                         cGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGt                                             >  1:2292958/1‑150 (MQ=255)

                                                                                                          gACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTc                                            >  1:2609109/1‑150 (MQ=255)

                                                                                                             cTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTGGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATg                                         >  1:4476330/1‑150 (MQ=255)

                                                                                                              tCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATgg                                        <  2:143280/150‑1 (MQ=255)

                                                                                                              tCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATgg                                        <  2:2079267/150‑1 (MQ=255)

                                                                                                               cGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGa                                       <  1:3655390/150‑1 (MQ=255)

                                                                                                                ggTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGAc                                      <  1:854466/150‑1 (MQ=255)

                                                                                                                   gtgGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTa                                   >  2:1940874/1‑150 (MQ=255)

                                                                                                                   gtgGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTa                                   >  2:3950174/1‑150 (MQ=255)

                                                                                                                     ggAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGc                                 <  2:1388272/150‑1 (MQ=255)

                                                                                                                       aaaTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCaa                               <  1:783962/150‑1 (MQ=255)

                                                                                                                       aaaTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCaa                               <  1:783936/150‑1 (MQ=255)

                                                                                                                        aaTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCaaa                              <  2:2419270/150‑1 (MQ=255)

                                                                                                                        aaTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCaaa                              <  1:1711265/150‑1 (MQ=255)

                                                                                                                         aTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAAc                             >  2:2003366/1‑150 (MQ=255)

                                                                                                                         aTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAAc                             <  2:540600/150‑1 (MQ=255)

                                                                                                                         aTTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAAc                             <  2:539724/150‑1 (MQ=255)

                                                                                                                                 cccGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCt                     >  2:2699557/1‑150 (MQ=255)

                                                                                                                                  ccGGAACTGGTTGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTg                    >  2:4272735/1‑150 (MQ=255)

                                                                                                                                     gAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGATCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt                 >  1:1137668/1‑150 (MQ=255)

                                                                                                                                     gAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGAt                 <  2:1265638/150‑1 (MQ=255)

                                                                                                                                      aaCTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATg                <  1:770520/150‑1 (MQ=255)

                                                                                                                                      aaCTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATg                <  1:770427/150‑1 (MQ=255)

                                                                                                                                         tGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTa             >  1:1584846/1‑150 (MQ=255)

                                                                                                                                         tGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTa             >  2:3894424/1‑150 (MQ=255)

                                                                                                                                         tGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTa             >  1:3268198/1‑150 (MQ=255)

                                                                                                                                          ggTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTAc            <  1:3607376/150‑1 (MQ=255)

                                                                                                                                          ggTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTAc            <  1:1005652/150‑1 (MQ=255)

                                                                                                                                           gTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACt           >  1:2798707/1‑150 (MQ=255)

                                                                                                                                             cGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGa         >  1:1780517/1‑150 (MQ=255)

                                                                                                                                             cGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGa         >  2:1507821/1‑150 (MQ=255)

                                                                                                                                              ggCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGAc        <  2:4432779/150‑1 (MQ=255)

                                                                                                                                              ggCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGAc        <  1:4494209/150‑1 (MQ=255)

                                                                                                                                                cATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGc      <  1:1985445/150‑1 (MQ=255)

                                                                                                                                                cATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGc      >  2:827055/1‑150 (MQ=255)

                                                                                                                                                  ttCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCtg    <  1:192286/150‑1 (MQ=255)

                                                                                                                                                  ttCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCtg    <  1:2490767/150‑1 (MQ=255)

                                                                                                                                                   tCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCtgt   >  2:533103/1‑150 (MQ=255)

                                                                                                                                                    cAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCtgtg  >  2:2588450/1‑150 (MQ=255)

TGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCTGTG  >  CP009273/1932553‑1932850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`