BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,982,943	A→T	14.2%	L10* (TTA→TAA)	yecH ←	DUF2492 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,982,943	0	A	T	14.2%	722.8 / 91.0	252	L10* (TTA→TAA)	yecH	DUF2492 family protein
Reads supporting (aligned to +/- strand): ref base A (114/102); new base T (9/27); total (123/129)
Fisher's exact test for biased strand distribution p-value = 2.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAACGTCGGTTTGAC  >  CP009273/1982794‑1983092

cTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGGATATTGCTCGCCTGATTCAATCATCATATTTa                                                                                                                                                       >  2:1013598/1‑150 (MQ=255)

cTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGACTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTa                                                                                                                                                       >  2:1889070/1‑150 (MQ=255)

     gctgcCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACt                                                                                                                                                  >  2:2832531/1‑150 (MQ=255)

      ctgcCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACtt                                                                                                                                                 <  1:276240/150‑1 (MQ=255)

      ctgcCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACtt                                                                                                                                                 >  2:2462941/1‑150 (MQ=255)

         ccAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCAt                                                                                                                                              >  2:301831/1‑150 (MQ=255)

         ccAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCAt                                                                                                                                              >  2:1641219/1‑150 (MQ=255)

         ccAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCAt                                                                                                                                              <  2:2772350/150‑1 (MQ=255)

          cAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATg                                                                                                                                             >  2:4169263/1‑150 (MQ=255)

            gAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGAc                                                                                                                                           <  2:3678777/150‑1 (MQ=255)

             aaaCGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGAcc                                                                                                                                          <  1:702631/150‑1 (MQ=255)

                      cAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCCATCATCATATTTAACACTTCATGACCGTGAATaga                                                                                                                                 >  2:1569665/1‑150 (MQ=255)

                      cAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATaga                                                                                                                                 >  1:2027658/1‑150 (MQ=255)

                            tcCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCAt                                                                                                                           <  2:4480150/150‑1 (MQ=255)

                              ccGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCg                                                                                                                         <  2:2699445/150‑1 (MQ=255)

                              ccGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCg                                                                                                                         <  1:1077347/150‑1 (MQ=255)

                                  tGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTccc                                                                                                                     <  2:3755142/150‑1 (MQ=255)

                                  tGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTccc                                                                                                                     >  2:2807307/1‑150 (MQ=255)

                                   gTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCcct                                                                                                                    <  1:4307929/150‑1 (MQ=255)

                                   gTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCcct                                                                                                                    <  2:4298854/150‑1 (MQ=255)

                                     cATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCcctcc                                                                                                                  <  1:3677379/150‑1 (MQ=255)

                                      aTCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCcctcct                                                                                                                 >  2:2536251/1‑150 (MQ=255)

                                      aTCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCcctcct                                                                                                                 >  2:1848405/1‑150 (MQ=255)

                                             cTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCaaaaaa                                                                                                          >  2:3207462/1‑150 (MQ=255)

                                              tGCCGAGCAGGTGTGAAAACGTGCCTGTTAACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCaaaaaaa                                                                                                         <  2:1279078/150‑1 (MQ=255)

                                                 cGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGcc                                                                                                      <  1:2806681/150‑1 (MQ=255)

                                                 cGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGcc                                                                                                      <  2:42233/150‑1 (MQ=255)

                                                  gAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTC‑AAAAAAGCCta                                                                                                    >  1:3775147/1‑150 (MQ=255)

                                                   aGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCta                                                                                                    <  2:2645783/150‑1 (MQ=255)

                                                     cAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagc                                                                                                  <  1:1175517/150‑1 (MQ=255)

                                                      aGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagcg                                                                                                 >  2:1712770/1‑150 (MQ=255)

                                                        gtgtGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagcgta                                                                                               <  2:1529094/150‑1 (MQ=255)

                                                        gtgtGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagcgta                                                                                               <  1:4150068/150‑1 (MQ=255)

                                                         tgtgAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagcgtag                                                                                              <  1:840724/150‑1 (MQ=255)

                                                           tgAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagcgtagcg                                                                                            <  1:2116146/150‑1 (MQ=255)

                                                           tgAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCtagcgtagcg                                                                                            >  2:201487/1‑150 (MQ=255)

                                                             aaaaCGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGAt                                                                                          >  2:3357962/1‑150 (MQ=255)

                                                              aaaCGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGAtt                                                                                         >  1:3000625/1‑150 (MQ=255)

                                                               aaCGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTg                                                                                        <  2:872088/150‑1 (MQ=255)

                                                                 cGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGcc                                                                                      <  1:2618907/150‑1 (MQ=255)

                                                                  gTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCg                                                                                     >  2:3554776/1‑150 (MQ=255)

                                                                  gTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCg                                                                                     >  2:1029289/1‑150 (MQ=255)

                                                                    gCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCt                                                                                   >  1:92922/1‑150 (MQ=255)

                                                                      cTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTa                                                                                 <  2:2568895/150‑1 (MQ=255)

                                                                      cTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTa                                                                                 <  2:2539344/150‑1 (MQ=255)

                                                                       tGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTAt                                                                                >  1:1296132/1‑150 (MQ=255)

                                                                       tGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTAt                                                                                <  1:2556354/150‑1 (MQ=255)

                                                                         ttCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGa                                                                              >  1:342963/1‑150 (MQ=255)

                                                                         ttCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGa                                                                              >  1:1888142/1‑150 (MQ=255)

                                                                             ccAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGAc                                                                          >  1:4370804/1‑150 (MQ=255)

                                                                               aaaaCGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACtt                                                                        >  1:647192/1‑150 (MQ=255)

                                                                                aaaCGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACttt                                                                       <  2:3118609/150‑1 (MQ=255)

                                                                                aaaCGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACttt                                                                       >  2:1053257/1‑150 (MQ=255)

                                                                                 aaCGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTg                                                                      <  1:2964806/150‑1 (MQ=255)

                                                                                 aaCGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTg                                                                      <  1:2965025/150‑1 (MQ=255)

                                                                                       cTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAg                                                                >  2:4204018/1‑150 (MQ=255)

                                                                                        tttAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAgc                                                               >  2:2889574/1‑150 (MQ=255)

                                                                                           aaTCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCa                                                            >  1:827622/1‑150 (MQ=255)

                                                                                            aTCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAg                                                           >  1:3198584/1‑150 (MQ=255)

                                                                                             tCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAgg                                                          <  1:201487/150‑1 (MQ=255)

                                                                                               gCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGAc                                                        >  2:1737266/1‑150 (MQ=255)

                                                                                                cAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACt                                                       >  2:2929183/1‑150 (MQ=255)

                                                                                                cAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACt                                                       <  1:2295186/150‑1 (MQ=255)

                                                                                                 aGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGTACTg                                                      <  2:1080707/150‑1 (MQ=255)

                                                                                                 aGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTg                                                      <  1:3723805/150‑1 (MQ=255)

                                                                                                    ttCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTTTGAAGACTTTGCGCCAGCGCAGGACTGAAt                                                   <  1:4461454/150‑1 (MQ=255)

                                                                                                      ccAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGc                                                 <  2:1009513/150‑1 (MQ=255)

                                                                                                      ccAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGc                                                 <  2:216091/150‑1 (MQ=255)

                                                                                                           cTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCttttt                                            <  2:3834304/150‑1 (MQ=255)

                                                                                                            tGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTa                                           >  1:1471860/1‑150 (MQ=255)

                                                                                                            tGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTa                                           >  1:286494/1‑150 (MQ=255)

                                                                                                            tGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTa                                           >  1:281343/1‑150 (MQ=255)

                                                                                                            tGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTa                                           >  1:2016981/1‑150 (MQ=255)

                                                                                                             ggCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTAt                                          <  1:115502/150‑1 (MQ=255)

                                                                                                             ggCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTAt                                          >  2:3315235/1‑150 (MQ=255)

                                                                                                             ggCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTAt                                          >  1:1185200/1‑150 (MQ=255)

                                                                                                              gCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTAtt                                         <  1:1062393/150‑1 (MQ=255)

                                                                                                                      atatTGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACAttt                                 <  1:3249949/150‑1 (MQ=255)

                                                                                                                       tatTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTta                                <  2:2027658/150‑1 (MQ=255)

                                                                                                                        atTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTtat                               >  1:512630/1‑150 (MQ=255)

                                                                                                                        atTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTtat                               <  1:1435287/150‑1 (MQ=255)

                                                                                                                         ttGCTCGCCTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTtata                              >  2:1019842/1‑150 (MQ=255)

                                                                                                                         ttGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTtata                              >  2:1344164/1‑150 (MQ=255)

                                                                                                                          tGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTatat                             <  2:4041854/150‑1 (MQ=255)

                                                                                                                            cTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATAttt                           <  2:2703507/150‑1 (MQ=255)

                                                                                                                            cTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATAttt                           <  1:2462942/150‑1 (MQ=255)

                                                                                                                             tCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTa                          >  2:572826/1‑150 (MQ=255)

                                                                                                                             tCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTa                          <  1:4479908/150‑1 (MQ=255)

                                                                                                                             tCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTa                          <  1:4481760/150‑1 (MQ=255)

                                                                                                                              cGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTac                         >  1:3411117/1‑150 (MQ=255)

                                                                                                                                 cTGATTCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACAcc                      <  1:1157366/150‑1 (MQ=255)

                                                                                                                                    aTTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCata                   >  2:2019128/1‑150 (MQ=255)

                                                                                                                                     ttCAATCATCATATTTTACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCatat                  <  1:1712770/150‑1 (MQ=255)

                                                                                                                                     ttCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCatat                  <  1:1830107/150‑1 (MQ=255)

                                                                                                                                          tcatcaTATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAAc             >  2:692302/1‑150 (MQ=255)

                                                                                                                                                tatTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTac                         >  1:4243804/1‑132 (MQ=255)

                                                                                                                                                 atTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAACGTCGGtt      >  1:3877218/1‑150 (MQ=255)

                                                                                                                                                  tttAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAACGTCGGttt     >  1:1577400/1‑150 (MQ=255)

                                                                                                                                                  tttAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAACGTCGGttt     >  1:177463/1‑150 (MQ=255)

                                                                                                                                                   ttAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAACGTCGGTTTg    >  1:2103965/1‑150 (MQ=255)

CTTTTGCTGCCAGAAACGCTACCAGCTCTCCCGCTGTCATCCCTTCTGCCGAGCAGGTGTGAAAACGTGCCTGTTCACCAAAACGCGCTTTAATCGCAGCTTCCAGACTGGCATGCGTATATTGCTCGCCTGATTCAATCATCATATTTAACACTTCATGACCGTGAATAGAGTCCATCGTCCCTCCTCAAAAAAAGCCTAGCGTAGCGATTGCCGCTTATGAAGACTTTGCGCCAGCGCAGGACTGAATGCTTTTTATTGTACATTTATATTTACACCATATGTAACGTCGGTTTGAC  >  CP009273/1982794‑1983092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`