Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,984,987 C→A 69.5% T38T (ACG→ACT)  yecA ← UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,984,9870CA69.5% 235.5 / 221.5 213T38T (ACG→ACT) yecAUPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base C (30/35);  new base A (70/78);  total (100/113)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 216 total aligned reads displayed.

TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984840‑1985136
                                                                                                                                                   |                                                                                                                                                     
     tATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACTGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATa                                                                                                                                                <  2:3734029/150‑1 (MQ=255)
     tATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATa                                                                                                                                                >  2:3750460/1‑150 (MQ=255)
      aTCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATaa                                                                                                                                               >  2:1353293/1‑150 (MQ=255)
      aTCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATaa                                                                                                                                               >  2:1352833/1‑150 (MQ=255)
        cGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAc                                                                                                                                             >  2:3217742/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAAcc                                                                                                                                            >  1:4354815/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAcc                                                                                                                                            >  2:1993145/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCAGAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGTCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAAcc                                                                                                                                            <  2:4321588/150‑1 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                         >  1:4454607/1‑150 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                         >  1:3601157/1‑150 (MQ=255)
             atatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTcc                                                                                                                                        <  2:3821351/150‑1 (MQ=255)
              tatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCa                                                                                                                                       >  2:1537712/1‑150 (MQ=255)
              tatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCa                                                                                                                                       <  2:3754759/150‑1 (MQ=255)
                  ttGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTc                                                                                                                                   <  2:1662563/150‑1 (MQ=255)
                  ttGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTc                                                                                                                                   <  1:2902558/150‑1 (MQ=255)
                        aGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCcac                                                                                                                             >  2:1380295/1‑150 (MQ=255)
                        aGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCcac                                                                                                                             >  1:1334160/1‑150 (MQ=255)
                            aGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCTATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCa                                                                                                                         <  2:1331359/150‑1 (MQ=255)
                            aGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCa                                                                                                                         <  2:4296230/150‑1 (MQ=255)
                               ttCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAgg                                                                                                                      >  1:1687819/1‑150 (MQ=255)
                                 cATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAt                                                                                                                    <  1:1727668/150‑1 (MQ=255)
                                  aTAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATg                                                                                                                   >  1:496711/1‑150 (MQ=255)
                                        cgcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                             <  1:2104741/150‑1 (MQ=255)
                                          cgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTc                                                                                                           >  2:3926474/1‑150 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                          >  1:1944474/1‑150 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                          <  1:859564/150‑1 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                          >  1:4112258/1‑150 (MQ=255)
                                             cATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgt                                                                                                        >  2:873335/1‑150 (MQ=255)
                                                  ctTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGtgttgt                                                                                                   <  2:3153061/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCCCACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:287277/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACCCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:2465094/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:1415653/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:285656/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                                 <  2:1470420/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                                 <  1:3842681/150‑1 (MQ=255)
                                                       tctGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTg                                                                                              <  1:1515301/150‑1 (MQ=255)
                                                             gCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGa                                                                                        >  1:2217670/1‑150 (MQ=255)
                                                              cccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGaa                                                                                       >  1:1284108/1‑150 (MQ=255)
                                                               ccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAat                                                                                      >  2:3024092/1‑150 (MQ=255)
                                                                 aGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAatat                                                                                    <  2:514529/150‑1 (MQ=255)
                                                                  gcgcGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATc                                                                                   <  1:1635868/150‑1 (MQ=255)
                                                                  gcgcGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATc                                                                                   <  1:3572712/150‑1 (MQ=255)
                                                                    gcgGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGt                                                                                 <  1:1608908/150‑1 (MQ=255)
                                                                      ggCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTcc                                                                               <  1:158449/150‑1 (MQ=255)
                                                                      ggCAAATAGTCAGCCCCACCCAACACTGAAACCAGCCACTGTTCAGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTcc                                                                               >  2:2901135/1‑150 (MQ=255)
                                                                        cacaTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAg                                                                             <  2:831884/150‑1 (MQ=255)
                                                                            tAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAt                                                                         <  1:667997/150‑1 (MQ=255)
                                                                             aGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAtt                                                                        >  2:2342881/1‑150 (MQ=255)
                                                                             aGTCAGCCCCACCCCACACGGCAACCAGCCACTGGTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATCAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAtt                                                                        <  1:2825951/150‑1 (MQ=255)
                                                                                  gCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAc                                                                   <  1:1566109/150‑1 (MQ=255)
                                                                                  gCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAc                                                                   <  1:1566289/150‑1 (MQ=255)
                                                                                   ccccaccacaCACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACt                                                                  <  2:3424917/150‑1 (MQ=255)
                                                                                     ccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCa                                                                <  1:4008499/150‑1 (MQ=255)
                                                                                     ccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCa                                                                >  1:3757897/1‑150 (MQ=255)
                                                                                     ccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCa                                                                <  1:231009/150‑1 (MQ=255)
                                                                                      caccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAc                                                               >  2:2320935/1‑150 (MQ=255)
                                                                                      caccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAc                                                               <  1:998628/150‑1 (MQ=255)
                                                                                        ccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACtt                                                             >  1:3899080/1‑150 (MQ=255)
                                                                                         cccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACttt                                                            <  2:3888042/150‑1 (MQ=255)
                                                                                            acacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGt                                                         >  1:568627/1‑150 (MQ=255)
                                                                                             cacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGtt                                                        >  1:4020058/1‑150 (MQ=255)
                                                                                             cacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGtt                                                        <  2:3067669/150‑1 (MQ=255)
                                                                                              acGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGttt                                                       >  1:3431259/1‑150 (MQ=255)
                                                                                               cGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTa                                                      <  2:562292/150‑1 (MQ=255)
                                                                                                ggCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTaa                                                     >  2:2563696/1‑150 (MQ=255)
                                                                                                  cAACCAGCCACTGTTCCGGTTCAGTCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACg                                                   >  1:3798593/1‑150 (MQ=255)
                                                                                                   aaCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACgg                                                  >  2:4373115/1‑150 (MQ=255)
                                                                                                     ccAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTc                                                <  1:3305966/150‑1 (MQ=255)
                                                                                                      cAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTcc                                               >  1:1618757/1‑150 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATCAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              <  1:2133191/150‑1 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              <  1:2133080/150‑1 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              >  2:1991181/1‑150 (MQ=255)
                                                                                                            cTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAACCCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGtttt                                         <  1:779603/150‑1 (MQ=255)
                                                                                                              gTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCa                                       >  1:2372832/1‑150 (MQ=255)
                                                                                                              gTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCa                                       >  1:2372954/1‑150 (MQ=255)
                                                                                                               ttCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCAt                                      >  2:2514401/1‑150 (MQ=255)
                                                                                                                tCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATa                                     <  2:903196/150‑1 (MQ=255)
                                                                                                                tCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATa                                     <  2:1860139/150‑1 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                >  1:3221162/1‑150 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                <  1:3591165/150‑1 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                <  1:3591142/150‑1 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                >  2:2160298/1‑150 (MQ=255)
                                                                                                                       cAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtttt                              <  1:3089078/150‑1 (MQ=255)
                                                                                                                        aaTCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTc                             <  2:4112258/150‑1 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           <  1:566990/150‑1 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           <  1:3138656/150‑1 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           <  1:4094737/150‑1 (MQ=255)
                                                                                                                             ctTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                        >  1:1760390/1‑150 (MQ=255)
                                                                                                                             ctTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                        >  1:2225609/1‑150 (MQ=255)
                                                                                                                             cgtGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                        <  2:1688481/148‑1 (MQ=255)
                                                                                                                                   gagaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtct                  >  1:4540270/1‑150 (MQ=255)
                                                                                                                                   gagaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACAACAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtct                  >  1:3923062/1‑150 (MQ=255)
                                                                                                                                      aaCTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCaa               <  1:4334395/150‑1 (MQ=255)
                                                                                                                                       aCTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAAc              <  1:267424/150‑1 (MQ=255)
                                                                                                                                         tCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTc            >  1:889536/1‑150 (MQ=255)
                                                                                                                                         tCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTc            <  2:3303238/150‑1 (MQ=255)
                                                                                                                                             cacCGCAGTCATTAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCa        <  1:3024089/150‑1 (MQ=255)
                                                                                                                                             cacCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCa        >  1:2905486/1‑150 (MQ=255)
                                                                                                                                                cgccgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGGTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCa     >  2:4303996/1‑150 (MQ=255)
                                                                                                                                                   cgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCt  <  2:1944474/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984840‑1985136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑