Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,991,843 G→A 7.1% L40F (CTC→TTC)  yecS ← ABC family putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,991,8430GA 7.1% 769.2 / 34.0 227L40F (CTC→TTC) yecSABC family putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base G (120/90);  new base A (6/10);  total (126/101)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 235 total aligned reads displayed.

GGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991695‑1991992
                                                                                                                                                    |                                                                                                                                                     
ggAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCgaga                                                                                                                                                      >  1:4533015/1‑150 (MQ=255)
  aaTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGAACATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAAt                                                                                                                                                    >  1:1006510/1‑150 (MQ=255)
   aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                   >  1:970337/1‑150 (MQ=255)
   aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                   <  2:604337/150‑1 (MQ=255)
    tGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaa                                                                                                                                                  <  2:2530589/150‑1 (MQ=255)
     gggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGACTaaa                                                                                                                                                 >  2:4182403/1‑150 (MQ=255)
     gggATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATaaa                                                                                                                                                 >  2:1090633/1‑150 (MQ=255)
          cTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGa                                                                                                                                            >  1:369674/1‑150 (MQ=255)
          cTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGa                                                                                                                                            >  1:3925758/1‑150 (MQ=255)
           tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           >  2:297/1‑150 (MQ=255)
           tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           >  1:2914347/1‑150 (MQ=255)
           tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           >  1:4238810/1‑150 (MQ=255)
                 gATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTaa                                                                                                                                     <  2:4356605/150‑1 (MQ=255)
                    aCCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGcc                                                                                                                                  >  2:1470485/1‑150 (MQ=255)
                     ccAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCa                                                                                                                                 >  1:429447/1‑150 (MQ=255)
                     ccAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCa                                                                                                                                 <  1:1340460/150‑1 (MQ=255)
                        aaCTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaaaa                                                                                                                              <  2:662037/150‑1 (MQ=255)
                           tGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAAc                                                                                                                           >  2:134580/1‑150 (MQ=255)
                           tGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAAc                                                                                                                           >  2:133450/1‑150 (MQ=255)
                             cGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACAt                                                                                                                         <  1:3302204/150‑1 (MQ=255)
                             cGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACAt                                                                                                                         <  2:3035720/150‑1 (MQ=255)
                             cGGCAGGCCGTAATAGATCATAAACAGTTGTGAGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACAt                                                                                                                         <  2:3646517/150‑1 (MQ=255)
                              ggCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATg                                                                                                                        >  1:2460143/1‑150 (MQ=255)
                                cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACGCGTGCAGTAAGCCAAAAAACATgcc                                                                                                                      <  2:1972134/150‑1 (MQ=255)
                                cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                      <  2:1971848/150‑1 (MQ=255)
                                cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                      <  2:2002281/150‑1 (MQ=255)
                                   gCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAACCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATgccgcc                                                                                                                   <  2:948147/150‑1 (MQ=255)
                                   gCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccgcc                                                                                                                   <  2:3896545/150‑1 (MQ=255)
                                      gTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGAt                                                                                                                >  2:4154886/1‑150 (MQ=255)
                                        aaTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATAc                                                                                                              >  1:2017597/1‑150 (MQ=255)
                                         aTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACt                                                                                                             <  1:4319505/150‑1 (MQ=255)
                                              tCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt                                                                                                        <  2:2284478/150‑1 (MQ=255)
                                               cATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTg                                                                                                       >  2:343854/1‑150 (MQ=255)
                                                 tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     <  2:1910311/150‑1 (MQ=255)
                                                    aCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCg                                                                                                  >  1:3476749/1‑150 (MQ=255)
                                                    aCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCg                                                                                                  <  1:2503053/150‑1 (MQ=255)
                                                     cAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGt                                                                                                 >  2:2793302/1‑150 (MQ=255)
                                                      aGTTGTGCGATTAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                >  2:2696027/1‑150 (MQ=255)
                                                      aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                <  2:1043821/150‑1 (MQ=255)
                                                      aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                >  1:1889196/1‑150 (MQ=255)
                                                          gtgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAcc                                                                                            >  1:1947846/1‑150 (MQ=255)
                                                            gCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCa                                                                                          <  1:2067774/150‑1 (MQ=255)
                                                               aTGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGcc                                                                                       >  1:1889936/1‑150 (MQ=255)
                                                               aTGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGcc                                                                                       <  2:2699481/150‑1 (MQ=255)
                                                                  aGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCt                                                                                    >  2:2462026/1‑150 (MQ=255)
                                                                  aGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCt                                                                                    >  2:2463106/1‑150 (MQ=255)
                                                                     ggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTc                                                                                 <  1:2664946/150‑1 (MQ=255)
                                                                     ggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTc                                                                                 <  1:3997968/150‑1 (MQ=255)
                                                                        gTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagc                                                                              <  1:2800988/150‑1 (MQ=255)
                                                                        gTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagc                                                                              <  1:516695/150‑1 (MQ=255)
                                                                         tACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagca                                                                             >  2:3140317/1‑150 (MQ=255)
                                                                          aCCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagcag                                                                            <  1:3788427/150‑1 (MQ=255)
                                                                          aCCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTATcagcag                                                                            >  2:3519483/1‑150 (MQ=255)
                                                                            cACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGa                                                                          >  2:906671/1‑150 (MQ=255)
                                                                            cACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGa                                                                          >  2:906534/1‑150 (MQ=255)
                                                                            cACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGa                                                                          >  1:1271670/1‑150 (MQ=255)
                                                                                  aaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCa                                                                    >  1:171696/1‑150 (MQ=255)
                                                                                  aaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCa                                                                    >  2:3467221/1‑150 (MQ=255)
                                                                                  aaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCa                                                                    <  1:3651054/150‑1 (MQ=255)
                                                                                   aaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCaa                                                                   >  2:4448521/1‑150 (MQ=255)
                                                                                   aaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCaa                                                                   >  2:2209759/1‑150 (MQ=255)
                                                                                    aTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAg                                                                  >  2:2435109/1‑150 (MQ=255)
                                                                                     tAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAgg                                                                 >  2:3741578/1‑150 (MQ=255)
                                                                                     tAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAgg                                                                 >  2:1480082/1‑150 (MQ=255)
                                                                                      agagATATAAAAACGCGCCAGCCTGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGa                                                                >  1:4457113/1‑150 (MQ=255)
                                                                                        agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  2:493883/150‑1 (MQ=255)
                                                                                        agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGACCGGCAAGGAAt                                                              >  1:3839054/1‑150 (MQ=255)
                                                                                         gaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATc                                                             >  1:303566/1‑150 (MQ=255)
                                                                                          atatAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCa                                                            <  1:1551987/150‑1 (MQ=255)
                                                                                           tataAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCaa                                                           <  1:2311140/150‑1 (MQ=255)
                                                                                                aaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAATCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAAcc                                                      >  2:1918874/1‑150 (MQ=255)
                                                                                                aaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAAcc                                                      <  1:2199893/150‑1 (MQ=255)
                                                                                                   cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGt                                                   >  2:4304764/1‑150 (MQ=255)
                                                                                                     cgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTg                                                 <  1:1443889/150‑1 (MQ=255)
                                                                                                        cagccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtat                                              <  1:4070798/150‑1 (MQ=255)
                                                                                                            cagcGCACGGTCCAGATTGGCGACAGACGCATCAGAGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCACTTTAAGCAGGAACGGCAAGGAATCAATAACCATTTGTATACtt                                          <  2:1570186/150‑1 (MQ=255)
                                                                                                            cagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACtt                                          <  1:1366073/150‑1 (MQ=255)
                                                                                                            cagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACtt                                          >  2:4451694/1‑150 (MQ=255)
                                                                                                               cgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCt                                       <  2:2691415/150‑1 (MQ=255)
                                                                                                                gcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                      >  1:4235526/1‑150 (MQ=255)
                                                                                                                gcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                      >  1:1699705/1‑150 (MQ=255)
                                                                                                                 cACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTg                                     >  1:844150/1‑150 (MQ=255)
                                                                                                                  aCGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGc                                    >  1:703746/1‑150 (MQ=255)
                                                                                                                    gggCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCAt                                  >  1:4027244/1‑150 (MQ=255)
                                                                                                                       ccAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCTAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAg                               <  1:2275324/150‑1 (MQ=255)
                                                                                                                        cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGc                              <  2:1195055/150‑1 (MQ=255)
                                                                                                                        cAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTATCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCaggcgc                              >  2:4320753/1‑145 (MQ=255)
                                                                                                                           aTTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGtt                           >  2:156340/1‑150 (MQ=255)
                                                                                                                            ttGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGttt                          <  1:2561119/150‑1 (MQ=255)
                                                                                                                              ggCGACAGACGCATCAGCGCGAGCATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTcc                        <  2:2478691/150‑1 (MQ=255)
                                                                                                                                cGACAGACGCATCAGCGCGAGAATAAACCCGCGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTCTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCgg                      <  2:3106446/150‑1 (MQ=255)
                                                                                                                                  aCAGACGCATCAGAGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAg                    <  2:32539/150‑1 (MQ=255)
                                                                                                                                     gACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                 <  1:148821/150‑1 (MQ=255)
                                                                                                                                        gCATCAGCGCGAGAATAACCCCGAGCAGTAAGCCAAAAACCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATCACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAAc              <  2:2534393/150‑1 (MQ=255)
                                                                                                                                        gCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAAc              <  1:3279002/150‑1 (MQ=255)
                                                                                                                                         cATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACg             >  1:1996343/1‑150 (MQ=255)
                                                                                                                                          aTCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGt            >  2:1749116/1‑150 (MQ=255)
                                                                                                                                            cAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGGCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgg          <  2:2913143/150‑1 (MQ=255)
                                                                                                                                                 cgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgatg     >  2:2820232/1‑150 (MQ=255)
                                                                                                                                                   agaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGACTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgatgat   >  2:3913128/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991695‑1991992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑