Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,038,769 C→A 13.3% V117V (GTC→GTA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,038,7690CA13.3% 800.9 / 66.8 245V117V (GTC→GTA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (113/99);  new base A (20/12);  total (133/112)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGG  >  CP009273/2038621‑2038918
                                                                                                                                                    |                                                                                                                                                     
cGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGGTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                                                                                                                                      <  2:1416157/150‑1 (MQ=255)
cGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTcc                                                                                                                                                      <  2:334971/150‑1 (MQ=255)
  gTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAATGAGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                                                                                                                                    <  2:572861/150‑1 (MQ=255)
  gTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                                                                                                                                    <  2:2860017/150‑1 (MQ=255)
      ccTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                                                                                                                                                >  2:2443506/1‑150 (MQ=255)
        tACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                                                                                                                              <  2:4085507/150‑1 (MQ=255)
        tACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                                                                                                                              <  2:1838692/150‑1 (MQ=255)
         acacCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAg                                                                                                                                             <  1:1961264/150‑1 (MQ=255)
             ccTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                                                                                                                         <  1:2378986/150‑1 (MQ=255)
             ccTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                                                                                                                         <  2:2408126/150‑1 (MQ=255)
                tGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGa                                                                                                                                      <  2:4276678/150‑1 (MQ=255)
                  gAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaa                                                                                                                                    >  1:2283417/1‑150 (MQ=255)
                    gCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa                                                                                                                                  <  1:2582304/150‑1 (MQ=255)
                        tGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                                                                                                                              >  1:283433/1‑150 (MQ=255)
                        tGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                                                                                                                              >  2:2099683/1‑150 (MQ=255)
                         ggAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAtt                                                                                                                             <  2:251560/150‑1 (MQ=255)
                           aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                                                                                                                           <  1:594373/150‑1 (MQ=255)
                             tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                                                                                                                         <  2:3740487/150‑1 (MQ=255)
                                  ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTGTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCccgcc                                                                                                                    >  2:3886501/1‑150 (MQ=255)
                                   cAAAGCGTTTCCGTACGTTTCGGTATTTCTGTGTCTTAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCACGAGGTTTTTTTATTGTACGCCAGGGTGATGAACTGTATGTCCAGGCTCAAGTTAGTGAAAATAAATTAACCccgccg                                                                                                                   <  2:1245529/150‑1 (MQ=255)
                                   cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccg                                                                                                                   <  2:142192/150‑1 (MQ=255)
                                   cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccg                                                                                                                   <  1:754119/150‑1 (MQ=255)
                                     aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgcc                                                                                                                 >  2:1927282/1‑150 (MQ=255)
                                     aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgcc                                                                                                                 >  2:1927257/1‑150 (MQ=255)
                                     aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgcc                                                                                                                 <  2:1633921/150‑1 (MQ=255)
                                     aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgcc                                                                                                                 <  1:1054650/150‑1 (MQ=255)
                                        cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg                                                                                                              >  2:3382938/1‑150 (MQ=255)
                                        cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGAGGAACTGGATGTCCCGGCCCAAGTGAGTGAAGAAAAATTAACCCCGCCGCCggg                                                                                                              >  2:3074443/1‑150 (MQ=255)
                                         gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATTTCCGCCAGGGTGATGAACTGCATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGt                                                                                                             >  1:2922167/1‑150 (MQ=255)
                                         gTTACCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAATAAATTAACCCCGCCGCCGGGt                                                                                                             >  2:1618920/1‑150 (MQ=255)
                                         gTTACCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGt                                                                                                             >  2:2022208/1‑150 (MQ=255)
                                            gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt                                                                                                          >  1:3899877/1‑150 (MQ=255)
                                                     ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAc                                                                                                 >  2:2705775/1‑150 (MQ=255)
                                                      tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACa                                                                                                <  2:2995844/150‑1 (MQ=255)
                                                       cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACaa                                                                                               >  1:116719/1‑150 (MQ=255)
                                                         gTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAAcc                                                                                             >  2:1072199/1‑150 (MQ=255)
                                                           aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTc                                                                                           >  1:3398373/1‑150 (MQ=255)
                                                           aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTc                                                                                           <  1:811960/150‑1 (MQ=255)
                                                           aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTc                                                                                           <  1:1894639/150‑1 (MQ=255)
                                                            tttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCg                                                                                          <  1:3468315/150‑1 (MQ=255)
                                                              tCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAg                                                                                        <  1:4076045/150‑1 (MQ=255)
                                                                ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTCATAGCCGTGACAACCTCGAGCa                                                                                      >  2:2727642/1‑150 (MQ=255)
                                                                ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCa                                                                                      >  2:2239201/1‑150 (MQ=255)
                                                                ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCa                                                                                      >  2:4476432/1‑150 (MQ=255)
                                                                   ggCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATCGCAGTGACAACCTCGAGCAACa                                                                                   >  2:3972862/1‑150 (MQ=255)
                                                                     cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGa                                                                                 <  1:3034905/150‑1 (MQ=255)
                                                                     cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGGGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGa                                                                                 >  2:533278/1‑150 (MQ=255)
                                                                     cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGa                                                                                 >  1:136563/1‑150 (MQ=255)
                                                                       gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATa                                                                               >  1:2449391/1‑150 (MQ=255)
                                                                       gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATa                                                                               <  1:2192868/150‑1 (MQ=255)
                                                                         gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGc                                                                             >  2:3490042/1‑150 (MQ=255)
                                                                          ttACGCAAACTCAACCAGATTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCAGCCGCAGGGTCATAGCAGTGACAACCTCGAGCAACAGATAGcc                                                                            >  2:2097865/1‑150 (MQ=255)
                                                                              gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTa                                                                        >  2:4497970/1‑150 (MQ=255)
                                                                                 aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACtt                                                                     >  2:3571031/1‑150 (MQ=255)
                                                                                  aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTc                                                                    <  1:1373060/150‑1 (MQ=255)
                                                                                   cTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTca                                                                   <  1:309141/150‑1 (MQ=255)
                                                                                     cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTcaca                                                                 <  2:2331038/150‑1 (MQ=255)
                                                                                      aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATACTGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAg                                                                <  2:3305330/150‑1 (MQ=255)
                                                                                      aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAg                                                                >  2:852951/1‑150 (MQ=255)
                                                                                         cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCaa                                                             >  1:2818645/1‑150 (MQ=255)
                                                                                         cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCaa                                                             >  1:2075889/1‑150 (MQ=255)
                                                                                          aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCaaa                                                            <  1:3446464/150‑1 (MQ=255)
                                                                                            tttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATc                                                          <  2:4100090/150‑1 (MQ=255)
                                                                                            tttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATc                                                          <  2:2582709/150‑1 (MQ=255)
                                                                                              tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCgg                                                        >  2:3446774/1‑150 (MQ=255)
                                                                                                gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGt                                                      >  2:2603205/1‑150 (MQ=255)
                                                                                                gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGt                                                      >  2:311505/1‑150 (MQ=255)
                                                                                                 tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGtc                                                     <  1:2436715/150‑1 (MQ=255)
                                                                                                    gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTctct                                                  >  2:1702089/1‑150 (MQ=255)
                                                                                                       tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCt                                               >  2:1080832/1‑150 (MQ=255)
                                                                                                          tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGc                                            >  1:1362290/1‑150 (MQ=255)
                                                                                                           cGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGcc                                           >  2:2955992/1‑150 (MQ=255)
                                                                                                            gAGTTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCg                                          >  1:4105856/1‑150 (MQ=255)
                                                                                                            gAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCg                                          <  2:2599281/150‑1 (MQ=255)
                                                                                                              ggTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGaa                                        >  1:138859/1‑150 (MQ=255)
                                                                                                               gTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTTAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAg                                       <  1:4310856/150‑1 (MQ=255)
                                                                                                                  ttGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGata                                    >  1:3157230/1‑150 (MQ=255)
                                                                                                                  ttGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGata                                    >  1:4241314/1‑150 (MQ=255)
                                                                                                                  ttGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGata                                    >  1:20098/1‑150 (MQ=255)
                                                                                                                  ttGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGata                                    >  1:20142/1‑150 (MQ=255)
                                                                                                                     aTAATGTCCGCCAGGGTGATGAACTGGATGTACCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATCGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGa                                 >  2:1954662/1‑150 (MQ=255)
                                                                                                                         tGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAg                             <  2:323140/150‑1 (MQ=255)
                                                                                                                           tCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCg                           <  2:384426/150‑1 (MQ=255)
                                                                                                                           tCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCg                           >  1:1598999/1‑150 (MQ=255)
                                                                                                                            ccGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGa                          <  1:346357/150‑1 (MQ=255)
                                                                                                                            ccGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGa                          <  1:365873/150‑1 (MQ=255)
                                                                                                                                cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCaa                      >  2:3392816/1‑150 (MQ=255)
                                                                                                                                cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCaa                      >  2:4371382/1‑150 (MQ=255)
                                                                                                                                cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCaa                      >  2:4371396/1‑150 (MQ=255)
                                                                                                                                cAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCaa                      <  1:516560/150‑1 (MQ=255)
                                                                                                                                 aGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAg                     >  2:2336147/1‑150 (MQ=255)
                                                                                                                                 aGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAg                     >  1:407630/1‑150 (MQ=255)
                                                                                                                                        tgaACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCaaa              <  1:2605260/150‑1 (MQ=255)
                                                                                                                                         gaACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGATCAACAGATATCCAGTAATTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAat             >  1:1799398/1‑150 (MQ=255)
                                                                                                                                         gaACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAat             >  1:653447/1‑150 (MQ=255)
                                                                                                                                            cTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATg          >  1:1011159/1‑150 (MQ=255)
                                                                                                                                              ggATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGgc        <  2:889687/150‑1 (MQ=255)
                                                                                                                                               gATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGgcg       >  1:2318663/1‑150 (MQ=255)
                                                                                                                                                   tCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTg   <  1:1200471/150‑1 (MQ=255)
                                                                                                                                                    cccGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGACAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTgg  >  2:706222/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACAACCTCGAGCAACAGATAGCCAGTACTTCACAGCAAATCGGGTCTCTGCTCGCCGAAGATATGAACAGCGAGCAAGCGGCAAATATGGCGCGTGG  >  CP009273/2038621‑2038918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑