Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,115,361 C→A 14.8% R367L (CGC→CTC)  cpsG ← phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,115,3610CA14.8% 868.2 / 92.4 277R367L (CGC→CTC) cpsGphosphomannomutase
Reads supporting (aligned to +/- strand):  ref base C (109/127);  new base A (20/21);  total (129/148)
Fisher's exact test for biased strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 284 total aligned reads displayed.

AGTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATA  >  CP009273/2115212‑2115510
                                                                                                                                                     |                                                                                                                                                     
aGTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTcgc                                                                                                                                                       >  1:3242930/1‑150 (MQ=255)
 gTCGGCAACGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTcgcg                                                                                                                                                      <  1:2525365/150‑1 (MQ=255)
   cGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTa                                                                                                                                                    >  1:3803644/1‑150 (MQ=255)
       aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAg                                                                                                                                                >  1:2272399/1‑150 (MQ=255)
       aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAg                                                                                                                                                >  1:3646193/1‑150 (MQ=255)
       aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAg                                                                                                                                                >  1:1744247/1‑150 (MQ=255)
       aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAg                                                                                                                                                <  2:2759470/150‑1 (MQ=255)
         aGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGtt                                                                                                                                              <  1:2886073/150‑1 (MQ=255)
         aGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGtt                                                                                                                                              <  1:2886293/150‑1 (MQ=255)
              aTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGccc                                                                                                                                         <  1:1158499/150‑1 (MQ=255)
              aTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTCCCAGTTCGccc                                                                                                                                         >  2:2571724/1‑150 (MQ=255)
                  tGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCg                                                                                                                                     <  1:1214212/150‑1 (MQ=255)
                   gATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGt                                                                                                                                    >  1:1619889/1‑150 (MQ=255)
                      gCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGtttt                                                                                                                                 <  2:973300/150‑1 (MQ=255)
                           cGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCtt                                                                                                                            <  1:1405486/150‑1 (MQ=255)
                           cGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGATGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCtt                                                                                                                            <  2:241848/150‑1 (MQ=255)
                            ggTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCttt                                                                                                                           >  2:3849535/1‑150 (MQ=255)
                               gCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAg                                                                                                                        <  2:2717857/150‑1 (MQ=255)
                               gCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAg                                                                                                                        <  2:3580195/150‑1 (MQ=255)
                                  aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGca                                                                                                                     <  2:1025191/150‑1 (MQ=255)
                                    ccACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcaca                                                                                                                   >  2:4002574/1‑150 (MQ=255)
                                    ccACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcaca                                                                                                                   >  2:418342/1‑150 (MQ=255)
                                    ccACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcaca                                                                                                                   >  2:418443/1‑150 (MQ=255)
                                     cACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTGAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCacac                                                                                                                  <  2:3248783/150‑1 (MQ=255)
                                      aCCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACAcc                                                                                                                 >  2:4542178/1‑150 (MQ=255)
                                        cGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAg                                                                                                               >  2:182447/1‑150 (MQ=255)
                                         gCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGt                                                                                                              >  1:553112/1‑150 (MQ=255)
                                          ccAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCAGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCCGtt                                                                                                             >  2:2528907/1‑150 (MQ=255)
                                          ccAGCGCCTCAATGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATATCACCGCTTGCAGGAAACGCCGCCATCCGTTCGCGTACCAGTTCGCCCAGCGTTTTATCTTACAGGCAAACAAGtt                                                                                                             >  2:229224/1‑150 (MQ=255)
                                           cAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTc                                                                                                            >  1:1424368/1‑150 (MQ=255)
                                            aGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCg                                                                                                           <  2:2784210/150‑1 (MQ=255)
                                             gcgcCTCACGGCTAAAATGCTGGTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCgg                                                                                                          <  2:2472201/150‑1 (MQ=255)
                                               gcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCg                                                                                                        <  2:2071820/150‑1 (MQ=255)
                                               gcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCg                                                                                                        <  1:1281476/150‑1 (MQ=255)
                                                 cTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGAc                                                                                                      <  1:1774773/150‑1 (MQ=255)
                                                     cGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagc                                                                                                  >  2:3763692/1‑150 (MQ=255)
                                                      ggCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagca                                                                                                 <  1:932865/150‑1 (MQ=255)
                                                      ggCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagca                                                                                                 <  1:1683121/150‑1 (MQ=255)
                                                         tAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGcc                                                                                              >  1:4366019/1‑150 (MQ=255)
                                                          aaaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCa                                                                                             <  1:1766793/150‑1 (MQ=255)
                                                           aaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCAc                                                                                            <  2:3117232/150‑1 (MQ=255)
                                                            aaTGCTGTTCCACGCGGTTAATCGCCACAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACg                                                                                           >  1:1060465/1‑150 (MQ=255)
                                                                 tGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATc                                                                                      >  1:2731017/1‑150 (MQ=255)
                                                                 tGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATc                                                                                      >  1:2229361/1‑150 (MQ=255)
                                                                     ccACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGc                                                                                  <  1:1061263/150‑1 (MQ=255)
                                                                      cACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGcc                                                                                 <  2:2030668/150‑1 (MQ=255)
                                                                      cACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGcc                                                                                 <  2:2029472/150‑1 (MQ=255)
                                                                           ggTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGt                                                                            <  1:4464569/150‑1 (MQ=255)
                                                                            gTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTc                                                                           <  1:2819487/150‑1 (MQ=255)
                                                                               aaTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCa                                                                        <  2:3334569/150‑1 (MQ=255)
                                                                               aaTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCa                                                                        >  2:4161830/1‑150 (MQ=255)
                                                                                  cGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTa                                                                     >  1:2115229/1‑150 (MQ=255)
                                                                                  cGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTa                                                                     <  2:1764973/150‑1 (MQ=255)
                                                                                   gCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTaa                                                                    >  2:4475234/1‑150 (MQ=255)
                                                                                     cTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGc                                                                  >  2:1171170/1‑150 (MQ=255)
                                                                                     cTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGc                                                                  >  2:219586/1‑150 (MQ=255)
                                                                                      tCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCg                                                                 <  2:187743/150‑1 (MQ=255)
                                                                                      tCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCg                                                                 <  1:3300908/150‑1 (MQ=255)
                                                                                        aaCGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGaa                                                               >  2:800493/1‑150 (MQ=255)
                                                                                         aCGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGaaa                                                              <  2:4286357/150‑1 (MQ=255)
                                                                                          cGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAAt                                                             <  1:2409602/150‑1 (MQ=255)
                                                                                           gggTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATc                                                            <  2:3663369/150‑1 (MQ=255)
                                                                                           gggTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATc                                                            <  1:4137596/150‑1 (MQ=255)
                                                                                                gcgcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGa                                                       >  1:3371593/1‑150 (MQ=255)
                                                                                                 cgcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa                                                      >  2:756959/1‑150 (MQ=255)
                                                                                                   ccAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGt                                                    <  2:3178948/150‑1 (MQ=255)
                                                                                                    cAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTa                                                   <  1:83805/150‑1 (MQ=255)
                                                                                                    cAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTa                                                   >  2:3457450/1‑150 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                  <  2:4284280/150‑1 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                  >  2:300228/1‑150 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                  >  2:1523725/1‑150 (MQ=255)
                                                                                                       tttGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtg                                                >  2:3796404/1‑150 (MQ=255)
                                                                                                         tGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggt                                              <  2:2228535/150‑1 (MQ=255)
                                                                                                           cTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggtgg                                            >  1:4152995/1‑150 (MQ=255)
                                                                                                           cTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggtgg                                            >  1:2702212/1‑150 (MQ=255)
                                                                                                                gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                       <  1:3946728/150‑1 (MQ=255)
                                                                                                                gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                       <  2:2461062/150‑1 (MQ=255)
                                                                                                                 aTCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTc                                      <  1:1781359/150‑1 (MQ=255)
                                                                                                                 aTCTCACCGCGTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTc                                      <  2:3625381/150‑1 (MQ=255)
                                                                                                                   ctcACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAt                                    <  1:2101354/150‑1 (MQ=255)
                                                                                                                     cACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAttt                                  <  1:4189670/150‑1 (MQ=255)
                                                                                                                     cACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAttt                                  <  1:4189579/150‑1 (MQ=255)
                                                                                                                       ccGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg                                <  1:911007/150‑1 (MQ=255)
                                                                                                                           ttGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGccac                            <  1:418347/150‑1 (MQ=255)
                                                                                                                               cGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATa                        >  2:3251507/1‑150 (MQ=255)
                                                                                                                               cGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATa                        >  1:2336729/1‑150 (MQ=255)
                                                                                                                                ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg                       >  1:1940184/1‑150 (MQ=255)
                                                                                                                                ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg                       >  2:878628/1‑150 (MQ=255)
                                                                                                                                ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATCGTGGGCGCTCAATTCGCCACCATAg                       >  2:4412591/1‑150 (MQ=255)
                                                                                                                                  aaaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGAt                     >  1:1556998/1‑150 (MQ=255)
                                                                                                                                        cgccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTc               >  2:1574541/1‑150 (MQ=255)
                                                                                                                                        cgccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTc               >  2:1574318/1‑150 (MQ=255)
                                                                                                                                        cgccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAGGGTGGGCGCTCATTTCGCCACCATAGATGGCGTc               >  2:4157058/1‑150 (MQ=255)
                                                                                                                                         gccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCt              >  2:3047359/1‑150 (MQ=255)
                                                                                                                                          ccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCtt             <  2:1619889/150‑1 (MQ=255)
                                                                                                                                             tCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCt          <  1:3797211/150‑1 (MQ=255)
                                                                                                                                             tCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCt          <  2:553112/150‑1 (MQ=255)
                                                                                                                                              ccGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt         <  2:2606135/150‑1 (MQ=255)
                                                                                                                                              ccGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt         >  2:1417531/1‑150 (MQ=255)
                                                                                                                                              ccGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt         <  2:1332676/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATA  >  CP009273/2115212‑2115510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑