| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 161,068 | A→C | 11.3% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 161,068 | 0 | A | C | 11.3% | 258.4 / 8.2 | 126 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (36/73); new base C (3/14); total (39/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > CP009273/160920‑161217 | cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt < 2:183020/150‑1 (MQ=255)cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt < 2:183025/150‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAATAGTATTCGTAAGTTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa > 1:318333/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 1:1458907/150‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 1:1458899/150‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 1:2823482/150‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCAAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 2:1958662/150‑1 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa > 1:875219/1‑150 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa > 2:1615904/1‑150 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCa > 2:1617287/1‑150 (MQ=255) aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:2584608/1‑150 (MQ=255) aatataTGAACGGGGGTTATCCCAAACTTGTCGGGCGGGACGACCGGGAAAATACTGCACCGCCGCGACGTACTAAAATTTATTCGTATGGTTGTTTTT‑CTCCCTCTCCTTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGtt < 2:809570/145‑1 (MQ=255) agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgg > 1:2417273/1‑148 (MQ=255) tGAATGGGCGTTATCCAAACCATGTCTGGCCGGACGACCCGGCAAATCCGGCACCGCCGCGACGGACGAAAATGTATTCGTATGGTTGGTTTT‑CCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccct < 2:2729983/150‑1 (MQ=37) tGAACGGGCGTTATCCCAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTATGGGTGGTTTT‑CTCCCCCTCCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcaccct < 2:2729994/150‑1 (MQ=38) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc > 1:4047527/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc > 1:4047505/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑ATCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaaccac > 2:1757881/1‑148 (MQ=255) tttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 2:2226211/149‑1 (MQ=255) gtaaTCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 2:297051/147‑1 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 2:296926/150‑1 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGCCCCCACAACCCAAc > 1:1715873/1‑150 (MQ=38) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCaaaa > 1:958371/1‑149 (MQ=38) ttATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:2332315/150‑1 (MQ=255) aTCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCCCCCAAAacca > 1:2077064/1‑149 (MQ=38) aTCCCAAACATGTCGGGCCGTACGACCCGGAAAATACTGCACCGACGCGACGTACGAAAAAGTTTTCGTAAGGTTGGTTTT‑CTCCCTCTCCGTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCct < 2:997357/150‑1 (MQ=38) ccccAAACTTGTCGGGCGGGACGACCCGGAAAAACCTCCACCGACGCCACGTACTAAAAATTATTCTTATGGTGGTTTTT‑CGCCCTCTCCCTGTGGGAGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc < 1:307401/149‑1 (MQ=255) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCAAAcccaca > 2:856692/1‑147 (MQ=38) aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTGGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt < 2:2630880/150‑1 (MQ=255) cTGGCCGTACGACCCGGAAATTAGTGCTCCGACGCTTGGGACTAAAATTTTGTGGTATGTTTGTTTTT‑CTCCCTCTCCGTGTGGGAGAGGTCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTTACCCTCTCCCTCAAGGga < 2:48075/150‑1 (MQ=255) cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTACGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 2:1751830/150‑1 (MQ=255) cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 2:4014339/150‑1 (MQ=255) gCCGGACGACCCGGCAAATACTCCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTT‑GTCCCTGTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 1:1810995/150‑1 (MQ=39) ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCGAAACCACTACCTCAAGGGAGAgggg > 2:1088188/1‑150 (MQ=37) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAGTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:616875/150‑1 (MQ=255) cgacCCGGCAAATACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc < 2:62461/150‑1 (MQ=255) cgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAACCCGCACCCGCCAGGGAGAGGGGTcc > 2:4321546/1‑150 (MQ=37) cgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTAAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc < 1:1267283/150‑1 (MQ=255) cgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTAAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc < 1:2771870/150‑1 (MQ=255) ccccGGCAAATACTGCCCCTACGCGACGGACTAAAAATTTTTCGTAAGGTTGGTTTT‑CCCCCTCACCCTTTGGGAGTGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa < 1:1965274/149‑1 (MQ=255) acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGGGCCCCCACCCCAAACCACACCCACAACGGGGGGGGGTCCgg > 1:2318402/1‑149 (MQ=25) acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCCAAACCCCACCCCCAAGGGAGTGGGGTcccg > 1:2320251/1‑148 (MQ=25) ccGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGTTTTA‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 1:3221345/150‑1 (MQ=255) ccggCACCGACGCGACGGACGAAAACGTAGGCGTACGGTTGGGTTT‑CGCCCTCTCCCTGTGGCAGAGGGCCGGGGTGCGGGCCTCCGCGCGCACGGTCACCCTCCCCCTAACCCTCTCCCTCACGGGAGCGGGGACCGAGCGAGCACaaa < 2:4393356/147‑1 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCGCCCCCTAACCCACACCCACAAGGGCGAGGGGGCCGGTCGAGCCCAAAttat > 2:3924175/1‑148 (MQ=37) cgacgcgacgGACGAAAAAGTATTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 1:4153515/150‑1 (MQ=255) cgacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 1:4153574/150‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCCCACATTTTgggag > 2:2395761/1‑150 (MQ=38) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTAACCCACACCCAAAACCACAACCTCAAGGGAGATGGGCACGATCGAGCCCAAATTTTgagag > 2:2395754/1‑150 (MQ=25) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGAGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag < 2:3762727/150‑1 (MQ=255) cgacgGACGAAAAATTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGata < 1:3702724/150‑1 (MQ=255) acggacgAAATAGTTTTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 2:3516277/150‑1 (MQ=255) acggacgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 2:3516402/150‑1 (MQ=255) acggacgAAAAAGTATGCGTACGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 2:3516280/150‑1 (MQ=255) acggacgAAAAAGCCATCGTAAGGTTGGGTTT‑CGCCCTCTCCCGGGGGGAGAGGGCCGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCCCCCTCACCCTCTCCCTCAAGGGAGAGGCGCCCGATCGAGCACAAATTTTGAGAGATAtc < 2:3516281/150‑1 (MQ=255) cgtacgAAAAATTTTTCGTAAGGTTGGTTTT‑CTCCCTCTCCCGGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 2:1242487/147‑1 (MQ=38) cggactAAAATGTATTCTTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 2:1241692/150‑1 (MQ=255) cggactAAAAATTTTTCTTATGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 2:1242435/150‑1 (MQ=38) cggacgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCGCTCCCACCACGGAGAGGGGCCCGATCGAGCACAAAATTTGAGAGatatat > 2:3949544/1‑148 (MQ=37) cggacgAAAAAGTAGTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 2:3900943/150‑1 (MQ=255) ggacgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtctt < 2:580069/150‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCACAAGGGAGAGGGGACCCATAGAGCACAAAATTTTAGAGATAtcatatta > 1:2612097/1‑144 (MQ=25) aaaaaGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 1:1709669/150‑1 (MQ=255) aaaaGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCGAACCCTCTCCCTCAACGGAGAGGGGACCGATCGAGCCCCAATTTTGAGAGATATATTCTTCTg > 2:2528384/1‑150 (MQ=38) aGTATTGGTATGGTTTGTTTT‑CTCCCTCTCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAAGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGATGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt < 1:3906651/150‑1 (MQ=38) ttCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa < 2:132866/150‑1 (MQ=255) cGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc < 2:969456/150‑1 (MQ=255) ttatgtttggttTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:655149/144‑1 (MQ=255) tacggttggttTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag < 1:3275004/147‑1 (MQ=255) tggttggttTT‑CTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTATCTTGTAACagaa < 1:2848725/149‑1 (MQ=255) tggtggttTTCCTCCCTCTCCGTGTGGGAGAGGGCGGGGGTTAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:795763/147‑1 (MQ=255) gttggttTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTGACCCTCACCCGAACCCACTCCCGCCAGGGAGAGGGGCCCGCGAGAGCCCCAAGTTTGGGGGTGATCTTCTTCTGTCTTGTAACaggaga > 2:3000434/1‑150 (MQ=21) gttggttTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCAAACCCAAACCCACAAGGGCGAGGGGTCCGATCGAGCCCACAATTTGAGAGATATATTATTATTTATTTTAAAataaga > 1:3839190/1‑150 (MQ=16) ggttTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACAACCTCAAGGGAGAGGGGACCGATCGAGCACAAAATTTTAGTGATATCATCTTCTGTCGTGTAACAgaagaaaa > 1:3706570/1‑148 (MQ=25) actccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaaa < 1:1700846/149‑1 (MQ=255) ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCTACCCCAAAACCACTACCACAAGGGTGAGGGGACCGATAGAGGACAAATTTTGAGAGATATCTTCTTCTGTATTGTAAAAGAATAACAGTAAAt > 2:4232030/1‑150 (MQ=25) ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAt < 2:2284711/150‑1 (MQ=255) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:2320013/150‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt < 2:4047527/150‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt < 2:4047505/150‑1 (MQ=255) cgccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt < 2:4047791/148‑1 (MQ=255) tccgtGTGGTTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtt < 1:3473715/150‑1 (MQ=255) tccgtGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtt < 1:3473688/150‑1 (MQ=255) cccgGTGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt < 1:3146750/150‑1 (MQ=255) cccgGTGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt < 2:1110507/150‑1 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg < 2:3985831/150‑1 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg < 2:3985944/150‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 2:716565/150‑1 (MQ=255) gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCTCACCCAAACCCAAACCCGCAAAGGGGAGGGGCCCCATAGAGCACAAAATTTGAGAGATATATTATTGTTTCTTTTAACAAAATCAAAGAAAATAGGGCTTTTTCGcc > 2:4349160/1‑150 (MQ=255) ggAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:1243020/150‑1 (MQ=255) gtgggCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa < 1:3640766/148‑1 (MQ=255) gtgggCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa < 1:3640765/148‑1 (MQ=255) gcgggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt < 2:958371/147‑1 (MQ=255) gTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgag < 1:856692/150‑1 (MQ=255) cgggCATCAGCGCGCACGTTCACCCTAACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa < 1:1983200/149‑1 (MQ=255) aGGGCATCAGCGCGCACGTTCACCCGCACCCAAAACCACACCCAAAAGGGAGAGGGGACCCATCGAGCACAAAATTTTAGATATATATTAATATGTATTTTAAAATAAAAAAAGAAAATAGGGCTTTTTAGCCAGAAAAATGAGGAGaaa > 2:309824/1‑150 (MQ=255) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg < 2:629341/150‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 1:2038038/150‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 2:1689866/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 2:2020724/150‑1 (MQ=255) | CGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > CP009273/160920‑161217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |