Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,043 C→A 70.4% W8L (TGG→TTG)  ccmD ← cytochrome c biogenesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,0430CA70.4% 281.7 / 247.2 244W8L (TGG→TTG) ccmDcytochrome c biogenesis protein
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base A (78/92);  total (113/131)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 244 total aligned reads displayed.

CTCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCA  >  CP009273/2288895‑2289190
                                                                                                                                                    |                                                                                                                                                   
  cACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAgg                                                                                                                                                  <  1:320852/150‑1 (MQ=255)
      cgcCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGc                                                                                                                                              <  1:2794230/150‑1 (MQ=255)
      cgcCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGc                                                                                                                                              <  1:2753699/150‑1 (MQ=255)
            cTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGc                                                                                                                                        <  1:880972/150‑1 (MQ=255)
               ttGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAgg                                                                                                                                     >  1:3458082/1‑150 (MQ=255)
                tGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAggg                                                                                                                                    <  2:1055372/150‑1 (MQ=255)
                  cgccacgccaCGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGt                                                                                                                                  <  2:3317319/150‑1 (MQ=255)
                     cacgccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCATAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCAt                                                                                                                               <  2:1873348/150‑1 (MQ=255)
                     cacgccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACAAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCAt                                                                                                                               >  2:4202246/1‑150 (MQ=255)
                        gccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTa                                                                                                                            <  1:4071080/150‑1 (MQ=255)
                        gccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTa                                                                                                                            >  1:1744356/1‑150 (MQ=255)
                        gccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTa                                                                                                                            >  1:3724201/1‑150 (MQ=255)
                         ccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTAc                                                                                                                           <  2:775935/150‑1 (MQ=255)
                              cAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCct                                                                                                                      <  1:2073058/150‑1 (MQ=255)
                                gAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCTCCAGACAGACTAAATAGGCGTAACCGCCCATTGCGAAAAATTCATTCTAGGAAGCAAATGCAGGGGTCATTTACGGCctct                                                                                                                    <  2:2477734/150‑1 (MQ=255)
                                   ttGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCtttt                                                                                                                 >  2:42263/1‑150 (MQ=255)
                                      cgcgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAg                                                                                                              >  1:3507737/1‑150 (MQ=255)
                                        cgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTa                                                                                                            <  1:2616790/150‑1 (MQ=255)
                                        cgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTa                                                                                                            <  1:3753536/150‑1 (MQ=255)
                                        cgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTa                                                                                                            <  1:3435999/150‑1 (MQ=255)
                                          aTGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATc                                                                                                          <  2:1678583/150‑1 (MQ=255)
                                           tGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCa                                                                                                         <  2:1941533/150‑1 (MQ=255)
                                           tGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCa                                                                                                         <  2:1190741/150‑1 (MQ=255)
                                            gTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTtttcagtttcag                                                                                                        <  1:3123586/150‑1 (MQ=255)
                                               gCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtc                                                                                                     <  2:4423331/150‑1 (MQ=255)
                                               gCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtc                                                                                                     <  2:4423369/150‑1 (MQ=255)
                                                  tCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcact                                                                                                  <  1:2691929/150‑1 (MQ=255)
                                                   cACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactc                                                                                                 >  1:183827/1‑150 (MQ=255)
                                                        aGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCa                                                                                            <  2:3462614/150‑1 (MQ=255)
                                                        aGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCa                                                                                            >  1:2313649/1‑150 (MQ=255)
                                                         gTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCac                                                                                           <  2:3848067/150‑1 (MQ=255)
                                                         gTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCac                                                                                           <  2:3848428/150‑1 (MQ=255)
                                                           gCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacgg                                                                                         <  1:2029623/150‑1 (MQ=255)
                                                            cACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacgga                                                                                        <  2:2276236/150‑1 (MQ=255)
                                                             aCGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggac                                                                                       <  1:2791814/150‑1 (MQ=255)
                                                             aCGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggac                                                                                       <  1:505368/150‑1 (MQ=255)
                                                               gACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacgg                                                                                     >  2:1731162/1‑150 (MQ=255)
                                                               gACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacgg                                                                                     >  2:2127319/1‑150 (MQ=255)
                                                               gACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacgg                                                                                     >  1:535920/1‑150 (MQ=255)
                                                                   aaaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                                 <  2:4511149/150‑1 (MQ=255)
                                                                   aaaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                                 <  1:4029350/150‑1 (MQ=255)
                                                                     aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtttt                                                                               >  1:2935802/1‑150 (MQ=255)
                                                                        caccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTcc                                                                            >  1:1632881/1‑150 (MQ=255)
                                                                           caGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATc                                                                         <  1:15790/150‑1 (MQ=255)
                                                                           caGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATc                                                                         <  2:2034641/150‑1 (MQ=255)
                                                                            aGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCa                                                                        >  1:257372/1‑150 (MQ=255)
                                                                            aGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCa                                                                        >  1:1228910/1‑150 (MQ=255)
                                                                               ggAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCa                                                                     <  1:129302/150‑1 (MQ=255)
                                                                                 aaTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaa                                                                   <  1:4427084/150‑1 (MQ=255)
                                                                                 aaTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaa                                                                   <  1:2554058/150‑1 (MQ=255)
                                                                                 aaTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaa                                                                   >  2:838021/1‑150 (MQ=255)
                                                                                  aTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaaa                                                                  >  1:1248988/1‑150 (MQ=255)
                                                                                      cGGTCATCACCACCGCCAGCCAGACAAAAAATGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCaa                                                              >  1:2695326/1‑150 (MQ=255)
                                                                                        gTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                            <  1:1950794/150‑1 (MQ=255)
                                                                                        gTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATCCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                            <  1:37252/150‑1 (MQ=255)
                                                                                         tcatcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCATAtt                                                           <  2:3657974/150‑1 (MQ=255)
                                                                                          catcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTa                                                          >  1:3538006/1‑150 (MQ=255)
                                                                                          catcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTa                                                          <  1:3844417/150‑1 (MQ=255)
                                                                                               ccaccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcat                                                     >  1:1105263/1‑150 (MQ=255)
                                                                                                caccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatc                                                    <  2:243359/150‑1 (MQ=255)
                                                                                                caccGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatc                                                    >  1:284057/1‑150 (MQ=255)
                                                                                                 accGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatcc                                                   <  1:3769532/150‑1 (MQ=255)
                                                                                                    gccagccaGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgca                                                >  2:2650186/1‑150 (MQ=255)
                                                                                                      cagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGACGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                              >  2:3623130/1‑150 (MQ=255)
                                                                                                      cagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                              >  2:641253/1‑150 (MQ=255)
                                                                                                      cagccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                              >  2:1656920/1‑150 (MQ=255)
                                                                                                       agccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtca                                             <  2:757634/150‑1 (MQ=255)
                                                                                                       agccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtca                                             >  1:3517574/1‑150 (MQ=255)
                                                                                                        gccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcag                                            >  1:2517776/1‑150 (MQ=255)
                                                                                                         ccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagc                                           >  1:3526501/1‑150 (MQ=255)
                                                                                                         ccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagc                                           <  2:1513495/150‑1 (MQ=255)
                                                                                                           agACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgt                                         <  1:3523541/150‑1 (MQ=255)
                                                                                                              cAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTAACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcag                                      >  1:4234387/1‑150 (MQ=255)
                                                                                                              cAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTAACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcag                                      >  1:4234172/1‑150 (MQ=255)
                                                                                                               aaaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                     >  2:1829118/1‑150 (MQ=255)
                                                                                                               aaaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                     >  1:2330181/1‑150 (MQ=255)
                                                                                                                aaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagcg                                    >  2:4268392/1‑150 (MQ=255)
                                                                                                                  aaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTCCGCATCCGCATCAGCGTCAGCGTg                                  <  2:466115/150‑1 (MQ=255)
                                                                                                                   aaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTgg                                 >  1:379733/1‑150 (MQ=255)
                                                                                                                   aaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTgg                                 >  1:4507915/1‑150 (MQ=255)
                                                                                                                    aGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGc                                <  2:4487606/150‑1 (MQ=255)
                                                                                                                    aGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGc                                <  2:1437706/150‑1 (MQ=255)
                                                                                                                       cGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGa                             <  2:2109707/150‑1 (MQ=255)
                                                                                                                       cGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGa                             >  1:3035637/1‑150 (MQ=255)
                                                                                                                       cGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGa                             >  1:753224/1‑150 (MQ=255)
                                                                                                                          aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                          <  2:3383555/150‑1 (MQ=255)
                                                                                                                          aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                          >  1:1712504/1‑150 (MQ=255)
                                                                                                                                ccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                    >  1:1025210/1‑150 (MQ=255)
                                                                                                                                ccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                    >  1:4138069/1‑150 (MQ=255)
                                                                                                                                ccATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAg                    >  1:4138468/1‑150 (MQ=255)
                                                                                                                                 cATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAgg                   >  2:3254834/1‑150 (MQ=255)
                                                                                                                                   ttGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGaa                 >  1:2819857/1‑150 (MQ=255)
                                                                                                                                    tGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAg                >  2:381393/1‑150 (MQ=255)
                                                                                                                                    tGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAg                >  2:3450700/1‑150 (MQ=255)
                                                                                                                                      cGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGcc              >  2:2581007/1‑150 (MQ=255)
                                                                                                                                            attcattcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAAt        >  2:4084276/1‑150 (MQ=255)
                                                                                                                                            attcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAAt        <  2:2838615/150‑1 (MQ=255)
                                                                                                                                            attcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAAt        <  1:3846854/150‑1 (MQ=255)
                                                                                                                                                  tcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACca  >  2:526011/1‑150 (MQ=255)
                                                                                                                                                  tcCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACca  >  2:527281/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCA  >  CP009273/2288895‑2289190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑