BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,353,889	C→A	9.8%	V335V (GTG→GTT)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,353,889	0	C	A	9.8%	833.3 / 42.5	231	V335V (GTG→GTT)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand): ref base C (113/95); new base A (11/12); total (124/107)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

AACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGT  >  CP009273/2353741‑2354038

aaCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCa                                                                                                                                                      <  2:2065797/150‑1 (MQ=255)

 aCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAc                                                                                                                                                     >  1:3721690/1‑150 (MQ=255)

 aCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAc                                                                                                                                                     <  1:2780713/150‑1 (MQ=255)

  ccGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAcc                                                                                                                                                    <  2:2342692/150‑1 (MQ=255)

  ccGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAcc                                                                                                                                                    >  1:3130800/1‑150 (MQ=255)

  ccGGGTTTATCAAGCACCGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAcc                                                                                                                                                    >  1:3130744/1‑150 (MQ=255)

   cGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCg                                                                                                                                                   >  2:4250010/1‑150 (MQ=255)

      gTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCAt                                                                                                                                                >  2:2616085/1‑150 (MQ=255)

           tCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTga                                                                                                                                           >  1:676978/1‑150 (MQ=255)

           tCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTga                                                                                                                                           >  1:3374988/1‑150 (MQ=255)

             aaGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTgaga                                                                                                                                         >  1:1018938/1‑150 (MQ=255)

             aaGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTgaga                                                                                                                                         <  1:902468/150‑1 (MQ=255)

               gCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGt                                                                                                                                       <  2:1945829/150‑1 (MQ=255)

               gCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGt                                                                                                                                       <  2:1946327/150‑1 (MQ=255)

                cACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTa                                                                                                                                      >  1:2090542/1‑150 (MQ=255)

                cACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTa                                                                                                                                      >  2:2754660/1‑150 (MQ=255)

                 aCTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTaa                                                                                                                                     <  2:3200568/150‑1 (MQ=255)

                     aTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAg                                                                                                                                 >  1:986425/1‑150 (MQ=255)

                       ttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGAc                                                                                                                               <  2:1452550/150‑1 (MQ=255)

                        tATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACg                                                                                                                              >  1:1653140/1‑150 (MQ=255)

                            aaTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAAcc                                                                                                                          >  2:3917679/1‑150 (MQ=255)

                            aaTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAAcc                                                                                                                          <  1:1871348/150‑1 (MQ=255)

                              tACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGt                                                                                                                        <  2:3324156/150‑1 (MQ=255)

                              tACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGt                                                                                                                        <  2:2840071/150‑1 (MQ=255)

                                   ccATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGc                                                                                                                   >  2:2577442/1‑150 (MQ=255)

                                    cATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGca                                                                                                                  >  2:3852971/1‑150 (MQ=255)

                                           cGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAg                                                                                                           >  2:1475286/1‑150 (MQ=255)

                                            ggAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGt                                                                                                          >  1:2884925/1‑150 (MQ=255)

                                            ggAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGt                                                                                                          >  1:2381556/1‑150 (MQ=255)

                                            ggAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGt                                                                                                          >  1:2381603/1‑150 (MQ=255)

                                             gAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGtt                                                                                                         <  2:461346/150‑1 (MQ=255)

                                              aaCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTg                                                                                                        <  2:843259/150‑1 (MQ=255)

                                                ccGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGcc                                                                                                      <  1:4368109/150‑1 (MQ=255)

                                                  ggCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGcccc                                                                                                    <  1:1958678/150‑1 (MQ=255)

                                                    cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                  <  2:3169500/150‑1 (MQ=255)

                                                    cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                  <  2:3832912/150‑1 (MQ=255)

                                                            gCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTgg                                                                                          >  1:1845507/1‑150 (MQ=255)

                                                               gAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGa                                                                                       >  2:3453593/1‑150 (MQ=255)

                                                               gAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGa                                                                                       >  2:3125953/1‑150 (MQ=255)

                                                                 aTCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGAtt                                                                                     >  2:670986/1‑150 (MQ=255)

                                                                    gggTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGTATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCg                                                                                  <  2:575282/150‑1 (MQ=255)

                                                                      gTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGc                                                                                >  2:2998133/1‑150 (MQ=255)

                                                                       tCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCa                                                                               <  2:2494586/150‑1 (MQ=255)

                                                                       tCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCa                                                                               <  1:2272703/150‑1 (MQ=255)

                                                                                ggACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacc                                                                      <  1:3543425/150‑1 (MQ=255)

                                                                                   cGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGc                                                                   <  1:2311930/150‑1 (MQ=255)

                                                                                   cGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGc                                                                   <  1:2311819/150‑1 (MQ=255)

                                                                                     cATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGt                                                                 >  1:3932600/1‑150 (MQ=255)

                                                                                      aTCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTg                                                                >  2:110937/1‑150 (MQ=255)

                                                                                        cGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGt                                                              >  1:4208171/1‑150 (MQ=255)

                                                                                        cGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGt                                                              <  1:1323363/150‑1 (MQ=255)

                                                                                        cGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGt                                                              <  1:74433/150‑1 (MQ=255)

                                                                                             aaCAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAac                                                         <  2:1841538/150‑1 (MQ=255)

                                                                                                aaTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccac                                                      >  1:2980954/1‑150 (MQ=255)

                                                                                                aaTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccac                                                      >  1:271139/1‑150 (MQ=255)

                                                                                                 aTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccacc                                                     <  2:3130769/150‑1 (MQ=255)

                                                                                                 aTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccacc                                                     <  2:3130813/150‑1 (MQ=255)

                                                                                                    cGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCa                                                  <  2:3422787/150‑1 (MQ=255)

                                                                                                      ggCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCAcc                                                >  1:4171910/1‑150 (MQ=255)

                                                                                                       gCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCa                                               <  2:301868/150‑1 (MQ=255)

                                                                                                       gCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCa                                               <  2:2262415/150‑1 (MQ=255)

                                                                                                        cTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAg                                              >  2:3565554/1‑150 (MQ=255)

                                                                                                            tcatcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCaa                                          <  2:2941650/150‑1 (MQ=255)

                                                                                                            tcatcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCaa                                          >  1:1461636/1‑150 (MQ=255)

                                                                                                             catcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAAc                                         >  2:884818/1‑150 (MQ=255)

                                                                                                               tcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACAt                                       <  2:145787/150‑1 (MQ=255)

                                                                                                                 aGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATcc                                     <  2:2647171/150‑1 (MQ=255)

                                                                                                                 aGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATcc                                     <  1:2627538/150‑1 (MQ=255)

                                                                                                                  ggAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCg                                    <  2:3963976/150‑1 (MQ=255)

                                                                                                                           tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATaa                           <  1:3307532/150‑1 (MQ=255)

                                                                                                                           tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATaa                           <  2:4419714/150‑1 (MQ=255)

                                                                                                                              aTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGt                        <  1:4382912/150‑1 (MQ=255)

                                                                                                                               tGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                       >  1:60191/1‑150 (MQ=255)

                                                                                                                               tGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                       <  1:56733/150‑1 (MQ=255)

                                                                                                                               tGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                       <  2:3690487/150‑1 (MQ=255)

                                                                                                                                ggCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCg                      >  1:3348964/1‑150 (MQ=255)

                                                                                                                                  cGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGAt                    >  2:3081647/1‑150 (MQ=255)

                                                                                                                                   gTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATa                   <  1:507636/150‑1 (MQ=255)

                                                                                                                                   gTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATa                   <  1:507490/150‑1 (MQ=255)

                                                                                                                                   gTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATa                   >  1:1768689/1‑150 (MQ=255)

                                                                                                                                     aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAcc                 >  1:1074854/1‑150 (MQ=255)

                                                                                                                                     aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAcc                 >  1:1073589/1‑150 (MQ=255)

                                                                                                                                       tGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCgg               >  1:3656159/1‑150 (MQ=255)

                                                                                                                                        ggTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGt              >  2:2015749/1‑150 (MQ=255)

                                                                                                                                         gTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGtt             >  1:1239620/1‑150 (MQ=255)

                                                                                                                                         gTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGTGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGtt             >  1:228359/1‑150 (MQ=255)

                                                                                                                                          tGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGttt            >  2:1426065/1‑150 (MQ=255)

                                                                                                                                          tGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGttt            >  1:241855/1‑150 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          >  1:2681151/1‑150 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          <  1:926632/150‑1 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          <  2:4195101/150‑1 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          >  1:2214566/1‑150 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          <  2:1443601/150‑1 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          >  1:1691728/1‑150 (MQ=255)

                                                                                                                                            aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          <  2:1946177/150‑1 (MQ=255)

                                                                                                                                              ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGa        >  1:825176/1‑150 (MQ=255)

                                                                                                                                                tGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGaaa      >  2:583990/1‑150 (MQ=255)

                                                                                                                                                   tCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCg   >  2:178566/1‑150 (MQ=255)

                                                                                                                                                    cACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGt  >  1:304405/1‑150 (MQ=255)

                                                                                                                                                    cACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGt  <  2:3237102/150‑1 (MQ=255)

AACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGT  >  CP009273/2353741‑2354038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`