Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,380,078 C→T 68.0% S247F (TCT→TTT)  yfbL → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,380,0780CT68.0% 383.9 / 278.5 278S247F (TCT→TTT) yfbLputative peptidase
Reads supporting (aligned to +/- strand):  ref base C (32/57);  new base T (87/101);  total (119/159)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 291 total aligned reads displayed.

TGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCG  >  CP009273/2379929‑2380227
                                                                                                                                                     |                                                                                                                                                     
tGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCAtc                                                                                                                                                       <  1:1237237/150‑1 (MQ=255)
tGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCAtc                                                                                                                                                       <  1:3509682/150‑1 (MQ=255)
tGGCTATTACGACTCTGCGCCTGGAAGCCAGAAGTACCCTTACCCGGCAAGGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCAtc                                                                                                                                                       <  1:3509246/150‑1 (MQ=255)
  gCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCAtttc                                                                                                                                                     <  2:1384676/150‑1 (MQ=255)
     aTTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAgg                                                                                                                                                  <  2:2923601/150‑1 (MQ=255)
      ttACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGa                                                                                                                                                 >  2:1088818/1‑150 (MQ=255)
      ttACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGa                                                                                                                                                 <  2:1931602/150‑1 (MQ=255)
        aCGCCTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAtt                                                                                                                                               <  2:739643/150‑1 (MQ=255)
        aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAtt                                                                                                                                               >  2:627380/1‑150 (MQ=255)
        aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAtt                                                                                                                                               <  2:3563306/150‑1 (MQ=255)
        aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAtt                                                                                                                                               <  2:2322363/150‑1 (MQ=255)
          gACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTa                                                                                                                                             <  1:1725620/150‑1 (MQ=255)
           aCTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTAt                                                                                                                                            <  1:4462006/150‑1 (MQ=255)
                 cgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGttt                                                                                                                                      <  1:3445511/150‑1 (MQ=255)
                 cgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGttt                                                                                                                                      <  1:226327/150‑1 (MQ=255)
                   ccTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTAt                                                                                                                                    <  2:1244113/150‑1 (MQ=255)
                    cTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTAtt                                                                                                                                   >  1:3940360/1‑150 (MQ=255)
                       gAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTa                                                                                                                                <  2:1820093/150‑1 (MQ=255)
                       gAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTa                                                                                                                                >  2:2395783/1‑150 (MQ=255)
                       gAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTa                                                                                                                                <  2:456281/150‑1 (MQ=255)
                       gAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTa                                                                                                                                <  2:101604/150‑1 (MQ=255)
                         aGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATg                                                                                                                              >  1:3636994/1‑150 (MQ=255)
                            cAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAAt                                                                                                                           <  1:4484620/150‑1 (MQ=255)
                            cAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAAt                                                                                                                           >  1:2961903/1‑150 (MQ=255)
                            cAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTGATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAAt                                                                                                                           <  1:515017/150‑1 (MQ=255)
                             aGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATa                                                                                                                          >  1:770785/1‑150 (MQ=255)
                              gAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAc                                                                                                                         <  1:1814672/150‑1 (MQ=255)
                              gAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAc                                                                                                                         <  2:3933728/150‑1 (MQ=255)
                                aTTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAccc                                                                                                                       <  2:2480687/150‑1 (MQ=255)
                                aTTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcac                                                                                                                       >  1:3781674/1‑148 (MQ=255)
                                 ttacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                      <  1:961085/150‑1 (MQ=255)
                                 ttacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                      <  1:2595946/150‑1 (MQ=255)
                                    cccttccccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTTTTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAgg                                                                                                                   <  2:2541532/150‑1 (MQ=255)
                                         acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTa                                                                                                              >  2:2669386/1‑150 (MQ=255)
                                         acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTa                                                                                                              >  1:257373/1‑150 (MQ=255)
                                            cGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTc                                                                                                           >  2:3719013/1‑150 (MQ=255)
                                             ggCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTcc                                                                                                          <  2:934035/150‑1 (MQ=255)
                                                aaTGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCgg                                                                                                       >  2:4426294/1‑150 (MQ=255)
                                                 aTGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGt                                                                                                      >  1:4157037/1‑150 (MQ=255)
                                                 aTGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGt                                                                                                      <  1:2276051/150‑1 (MQ=255)
                                                 aTGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGt                                                                                                      <  1:4161051/150‑1 (MQ=255)
                                                  tGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTa                                                                                                     >  2:2978668/1‑150 (MQ=255)
                                                  tGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTa                                                                                                     <  1:849712/150‑1 (MQ=255)
                                                  tGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTa                                                                                                     >  2:1634699/1‑150 (MQ=255)
                                                  tGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTa                                                                                                     <  1:3433679/150‑1 (MQ=255)
                                                    tCCTGGCTTTATCCCGATCGTGGAGACTTTATTGCCGTTGTCGTCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGAGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGTTAtt                                                                                                   <  2:2884782/150‑1 (MQ=255)
                                                    tCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTAtt                                                                                                   <  2:2010981/150‑1 (MQ=255)
                                                    tCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTAtt                                                                                                   <  2:2254172/150‑1 (MQ=255)
                                                    tCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTAtt                                                                                                   <  2:558420/150‑1 (MQ=255)
                                                      cTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGa                                                                                                 <  2:2362160/150‑1 (MQ=255)
                                                             tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTctct                                                                                          <  1:4403537/150‑1 (MQ=255)
                                                              aTCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTg                                                                                         >  2:337840/1‑150 (MQ=255)
                                                               tCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGa                                                                                        >  1:482004/1‑150 (MQ=255)
                                                                 ccGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGacc                                                                                      >  2:1070150/1‑150 (MQ=255)
                                                                  cGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGacca                                                                                     <  2:4054202/150‑1 (MQ=255)
                                                                      cGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTg                                                                                 <  1:4268946/150‑1 (MQ=255)
                                                                      cGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTg                                                                                 >  2:3022495/1‑150 (MQ=255)
                                                                      cGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTg                                                                                 <  1:3755118/150‑1 (MQ=255)
                                                                       ggggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGa                                                                                >  1:1097840/1‑150 (MQ=255)
                                                                           agaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAatta                                                                            <  1:1519609/150‑1 (MQ=255)
                                                                            gaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAATAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAattat                                                                           <  1:3093074/150‑1 (MQ=255)
                                                                            gaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAattat                                                                           <  1:3092927/150‑1 (MQ=255)
                                                                             aCTTTATTGCCGTGGTCGGCAGAATACATGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAattatt                                                                          <  2:245917/150‑1 (MQ=255)
                                                                                 tATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCa                                                                      >  2:3308649/1‑150 (MQ=255)
                                                                                 tATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCa                                                                      <  2:11799/150‑1 (MQ=255)
                                                                                  aTTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCaa                                                                     >  1:3043860/1‑150 (MQ=255)
                                                                                      ccGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACg                                                                 <  1:3413374/150‑1 (MQ=255)
                                                                                       cGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGa                                                                >  1:4056553/1‑150 (MQ=255)
                                                                                        gTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGat                                                               <  2:2285736/150‑1 (MQ=255)
                                                                                        gTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGat                                                               <  1:4167519/150‑1 (MQ=255)
                                                                                        gTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGat                                                               <  1:2052356/150‑1 (MQ=255)
                                                                                        gTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGat                                                               <  2:257373/150‑1 (MQ=255)
                                                                                                cAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGc                                                       >  1:191737/1‑150 (MQ=255)
                                                                                                  gAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCa                                                     >  1:2907811/1‑150 (MQ=255)
                                                                                                    aTACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATa                                                   <  2:142766/150‑1 (MQ=255)
                                                                                                     tACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATaa                                                  <  1:3342295/150‑1 (MQ=255)
                                                                                                       cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATg                                                <  1:284272/150‑1 (MQ=255)
                                                                                                          gATACCAACGCCGTTCGTCAGGTAAAAGCGGCATTGGTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTAGTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAtt                                             >  2:3187116/1‑150 (MQ=255)
                                                                                                               cAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGa                                        >  1:2524937/1‑150 (MQ=255)
                                                                                                               cAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGa                                        <  1:2166661/150‑1 (MQ=255)
                                                                                                               cAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGa                                        <  1:4071949/150‑1 (MQ=255)
                                                                                                                aaCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGac                                       <  2:3820134/150‑1 (MQ=255)
                                                                                                                 aCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACTCGATATTCCCGCCATAATGATTACTGaca                                      >  1:4423688/1‑150 (MQ=255)
                                                                                                                  cGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGacac                                     <  1:655407/150‑1 (MQ=255)
                                                                                                                  cGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGacac                                     <  1:2936749/150‑1 (MQ=255)
                                                                                                                    ccGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATGTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCg                                   <  2:3866420/150‑1 (MQ=255)
                                                                                                                       ttCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCtt                                >  2:3666492/1‑150 (MQ=255)
                                                                                                                          gTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCttttt                             >  1:4250602/1‑150 (MQ=255)
                                                                                                                           tCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTa                            >  1:3943016/1‑150 (MQ=255)
                                                                                                                                  aaaGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaat                     <  1:2851605/150‑1 (MQ=255)
                                                                                                                                     gCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATaaa                  >  2:1374633/1‑150 (MQ=255)
                                                                                                                                     gCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATaaa                  <  1:2445879/150‑1 (MQ=255)
                                                                                                                                       ggCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACa                >  2:4236572/1‑150 (MQ=255)
                                                                                                                                         cATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAAt              <  1:127822/150‑1 (MQ=255)
                                                                                                                                          aTTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATa             >  2:1683244/1‑150 (MQ=255)
                                                                                                                                                gtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACtt       >  1:509489/1‑150 (MQ=255)
                                                                                                                                                 tCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTg      >  1:1186212/1‑150 (MQ=255)
                                                                                                                                                 tCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTg      <  2:80190/150‑1 (MQ=255)
                                                                                                                                                  cATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGc     <  2:1577906/150‑1 (MQ=255)
                                                                                                                                                     ctcAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCg  <  1:1318241/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCG  >  CP009273/2379929‑2380227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑