Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 183,820 G→T 6.6% R440S (CGC→AGC)  glnD ← uridylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273183,8200GT 6.6% 1013.3 / 31.5 270R440S (CGC→AGC) glnDuridylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (137/115);  new base T (8/10);  total (145/125)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 274 total aligned reads displayed.

CTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGG  >  CP009273/183671‑183969
                                                                                                                                                     |                                                                                                                                                     
  ggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCACACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGAt                                                                                                                                                     <  1:1514620/150‑1 (MQ=255)
    cgcgTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGc                                                                                                                                                   <  2:1596197/150‑1 (MQ=255)
    cgcgTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGc                                                                                                                                                   <  1:755756/150‑1 (MQ=255)
       gTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCAtt                                                                                                                                                <  2:3935593/150‑1 (MQ=255)
        tttCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCTATGCATTg                                                                                                                                               <  1:2481235/150‑1 (MQ=255)
        tttCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGGTCATCCACCGTGTAGGCGTGGAACAGATCAAACAGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATTGCGATGCATTg                                                                                                                                               >  1:1791059/1‑150 (MQ=255)
         ttcttcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCAGATACGCGCCGAGCACGCTATGGCGATGCATTgg                                                                                                                                              >  2:2595426/1‑150 (MQ=255)
          tcttcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGc                                                                                                                                             >  1:982430/1‑150 (MQ=255)
           cttcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCa                                                                                                                                            >  2:3625324/1‑150 (MQ=255)
           cttcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCa                                                                                                                                            >  2:1724347/1‑150 (MQ=255)
            ttcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCaa                                                                                                                                           <  2:1205419/150‑1 (MQ=255)
                    cAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCcgc                                                                                                                                   <  1:3652200/150‑1 (MQ=255)
                      aaaCTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCgcgc                                                                                                                                 >  2:1104919/1‑150 (MQ=255)
                       aaCTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGcgcc                                                                                                                                <  2:3214395/150‑1 (MQ=255)
                               aGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCTATGCATTGGCAATAGCCCGCGCCGCACCGCt                                                                                                                        >  1:213547/1‑150 (MQ=255)
                                 tttCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTcc                                                                                                                      <  1:570988/150‑1 (MQ=255)
                                   tCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCgg                                                                                                                    >  2:1419215/1‑150 (MQ=255)
                                   tCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCgg                                                                                                                    <  2:808192/150‑1 (MQ=255)
                                   tCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCgg                                                                                                                    >  2:3685202/1‑150 (MQ=255)
                                       cATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTg                                                                                                                <  1:1826993/150‑1 (MQ=255)
                                       cATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTg                                                                                                                >  1:2232346/1‑150 (MQ=255)
                                           aCGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGc                                                                                                            >  1:1906490/1‑150 (MQ=255)
                                              cgGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGa                                                                                                         >  2:3340889/1‑150 (MQ=255)
                                               ggATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGaa                                                                                                        <  2:3827895/150‑1 (MQ=255)
                                               ggATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGaa                                                                                                        <  2:1766419/150‑1 (MQ=255)
                                                 aTAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATg                                                                                                      >  1:615418/1‑150 (MQ=255)
                                                  tAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGc                                                                                                     <  1:328417/150‑1 (MQ=255)
                                                   aGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCt                                                                                                    <  1:3038153/150‑1 (MQ=255)
                                                   aGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCt                                                                                                    <  2:3567394/150‑1 (MQ=255)
                                                   aGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCt                                                                                                    >  2:3988577/1‑150 (MQ=255)
                                                   aGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCt                                                                                                    >  2:1884997/1‑150 (MQ=255)
                                                   aGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCt                                                                                                    <  1:1390366/150‑1 (MQ=255)
                                                    gTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTc                                                                                                   <  1:3963950/150‑1 (MQ=255)
                                                          tCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAAc                                                                                             <  1:1509644/150‑1 (MQ=255)
                                                             tCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGt                                                                                          >  2:2430972/1‑150 (MQ=255)
                                                              ccACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtt                                                                                         >  1:3522662/1‑150 (MQ=255)
                                                              ccACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtt                                                                                         >  2:2164339/1‑150 (MQ=255)
                                                               cACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGttt                                                                                        <  1:4473779/150‑1 (MQ=255)
                                                               cACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGttt                                                                                        <  1:817131/150‑1 (MQ=255)
                                                               cACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGttt                                                                                        <  1:817115/150‑1 (MQ=255)
                                                                aCCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCTATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtttt                                                                                       <  1:2005938/150‑1 (MQ=255)
                                                                aCCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCTATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtttt                                                                                       <  1:747809/150‑1 (MQ=255)
                                                                aCCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtttt                                                                                       <  2:2188753/150‑1 (MQ=255)
                                                                aCCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAAAAGtttt                                                                                       >  2:802712/1‑150 (MQ=255)
                                                                 ccGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTc                                                                                      >  1:1974172/1‑150 (MQ=255)
                                                                  cGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCg                                                                                     >  2:1527959/1‑150 (MQ=255)
                                                                  cGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCg                                                                                     >  2:1060662/1‑150 (MQ=255)
                                                                    tgtAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTg                                                                                   >  2:392875/1‑150 (MQ=255)
                                                                    tgtAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTg                                                                                   >  1:2576327/1‑150 (MQ=255)
                                                                     gtAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGc                                                                                  <  2:575156/150‑1 (MQ=255)
                                                                     gtAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGc                                                                                  <  2:3098159/150‑1 (MQ=255)
                                                                     gtAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGc                                                                                  <  2:3098230/150‑1 (MQ=255)
                                                                      tAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCt                                                                                 <  2:3016036/150‑1 (MQ=255)
                                                                       aGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCtt                                                                                >  2:2354098/1‑150 (MQ=255)
                                                                       aGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCtt                                                                                >  2:2355177/1‑150 (MQ=255)
                                                                               aaCAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGGACGCTAAGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAata                                                                        <  1:3691880/150‑1 (MQ=255)
                                                                                aCAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAatat                                                                       <  1:4044557/150‑1 (MQ=255)
                                                                                 cAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATAtt                                                                      >  1:4219247/1‑150 (MQ=255)
                                                                                 cAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATAtt                                                                      >  1:497562/1‑150 (MQ=255)
                                                                                  aGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTa                                                                     >  1:4077758/1‑150 (MQ=255)
                                                                                   gATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTac                                                                    >  2:2282465/1‑150 (MQ=255)
                                                                                       aaaCTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAg                                                                >  1:3093372/1‑150 (MQ=255)
                                                                                       aaaCTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAg                                                                <  2:1906490/150‑1 (MQ=255)
                                                                                       aaaCTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAg                                                                >  2:2503490/1‑150 (MQ=255)
                                                                                         aCTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCg                                                              <  2:1169146/150‑1 (MQ=255)
                                                                                         aCTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCg                                                              >  1:1680812/1‑150 (MQ=255)
                                                                                           tGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCTATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGgt                                                            >  2:130425/1‑150 (MQ=255)
                                                                                            gCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGgtt                                                           >  2:2484441/1‑150 (MQ=255)
                                                                                            gCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGgtt                                                           <  1:2423802/150‑1 (MQ=255)
                                                                                             cATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGgttg                                                          <  2:642682/150‑1 (MQ=255)
                                                                                              aTCTGCCCGACGAGATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGgttgt                                                         <  2:240957/150‑1 (MQ=255)
                                                                                                 tGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGc                                                      >  1:3848167/1‑150 (MQ=255)
                                                                                                  gCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCa                                                     <  2:2347701/150‑1 (MQ=255)
                                                                                                   cccGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAg                                                    >  1:1571701/1‑150 (MQ=255)
                                                                                                   cccGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAg                                                    >  1:4021931/1‑150 (MQ=255)
                                                                                                   cccGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAg                                                    >  1:4342233/1‑150 (MQ=255)
                                                                                                      gacgaTATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATg                                                 <  1:3648628/150‑1 (MQ=255)
                                                                                                       acgaTATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCTATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATgg                                                <  1:444515/150‑1 (MQ=255)
                                                                                                         gaTATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCg                                              <  2:1026222/150‑1 (MQ=255)
                                                                                                          atatGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGa                                             >  1:2558136/1‑150 (MQ=255)
                                                                                                            atGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACg                                           >  1:124474/1‑150 (MQ=255)
                                                                                                            atGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACg                                           >  2:4473163/1‑150 (MQ=255)
                                                                                                             tGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACgg                                          >  1:4391547/1‑150 (MQ=255)
                                                                                                             tGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACgg                                          >  1:3411394/1‑150 (MQ=255)
                                                                                                              gCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACggg                                         >  1:4315727/1‑150 (MQ=255)
                                                                                                              gCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACggg                                         <  2:708506/150‑1 (MQ=255)
                                                                                                                 aCCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCAt                                      >  2:2669855/1‑150 (MQ=255)
                                                                                                                    aTTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCTCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGTTTGTTGCAGATGGCGACGGGCATGGc                                   <  2:4354407/150‑1 (MQ=255)
                                                                                                                    aTTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGc                                   <  2:2028628/150‑1 (MQ=255)
                                                                                                                    aTTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGATTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGc                                   <  2:2029086/150‑1 (MQ=255)
                                                                                                                       gCGGCATCTACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGACTGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTa                                <  2:2731156/150‑1 (MQ=255)
                                                                                                                          gCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACt                             <  2:4166760/150‑1 (MQ=255)
                                                                                                                           cATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTg                            >  1:3074908/1‑150 (MQ=255)
                                                                                                                                  gcgcCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGc                     <  2:1234634/150‑1 (MQ=255)
                                                                                                                                    gcCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCgt                   >  1:4147639/1‑150 (MQ=255)
                                                                                                                                        aGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCgtggtg               >  1:61277/1‑150 (MQ=255)
                                                                                                                                                aTGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAAt       >  1:1196267/1‑150 (MQ=255)
                                                                                                                                                 tGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATg      <  2:4115815/150‑1 (MQ=255)
                                                                                                                                                 tGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATg      >  1:3920697/1‑150 (MQ=255)
                                                                                                                                                     gATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCgg  >  2:3376109/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGG  >  CP009273/183671‑183969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑