Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,498,834 C→A 12.6% E65* (GAA→TAA)  ypdE ← aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,498,8340CA12.6% 956.0 / 78.7 285E65* (GAA→TAA) ypdEaminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (124/125);  new base A (19/17);  total (143/142)
Fisher's exact test for biased strand distribution p-value = 8.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 285 total aligned reads displayed.

TCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCG  >  CP009273/2498686‑2498982
                                                                                                                                                    |                                                                                                                                                    
tctccGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCg                                                                                                                                                     >  2:493208/4‑150 (MQ=255)
 cGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGt                                                                                                                                                    >  1:2115198/1‑150 (MQ=255)
   ccGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTcc                                                                                                                                                  >  2:3751800/1‑150 (MQ=255)
     gTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCat                                                                                                                                                <  2:2082183/150‑1 (MQ=255)
     gTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCat                                                                                                                                                <  2:2082812/150‑1 (MQ=255)
     gTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCat                                                                                                                                                <  2:3277331/150‑1 (MQ=255)
      tCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCata                                                                                                                                               >  1:2904734/1‑150 (MQ=255)
      tCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCata                                                                                                                                               <  1:1716814/150‑1 (MQ=255)
       cAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCatat                                                                                                                                              >  2:4486213/1‑150 (MQ=255)
             ggCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGcac                                                                                                                                        <  2:1639497/150‑1 (MQ=255)
              gCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGcaca                                                                                                                                       <  1:887494/150‑1 (MQ=255)
                    aaTTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGatcat                                                                                                                                 <  1:1763180/150‑1 (MQ=255)
                    aaTTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGatcat                                                                                                                                 <  2:4251093/150‑1 (MQ=255)
                         tGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCt                                                                                                                            >  2:4369723/1‑150 (MQ=255)
                          gCACTCGTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCtt                                                                                                                           <  2:3830175/150‑1 (MQ=255)
                           cACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTc                                                                                                                          >  1:2033600/1‑150 (MQ=255)
                           cACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTc                                                                                                                          >  1:2033668/1‑150 (MQ=255)
                               ctTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGAc                                                                                                                      >  2:228261/1‑150 (MQ=255)
                               ctTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGAc                                                                                                                      <  1:1187739/150‑1 (MQ=255)
                               ctTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGAc                                                                                                                      >  1:689941/1‑150 (MQ=255)
                               ctTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGAc                                                                                                                      >  2:979299/1‑150 (MQ=255)
                                ttCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGAcc                                                                                                                     >  1:736841/1‑150 (MQ=255)
                                 tCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCt                                                                                                                    >  1:1033752/1‑150 (MQ=255)
                                   aCGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGt                                                                                                                  >  2:4022958/1‑150 (MQ=255)
                                      ggtggtGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGa                                                                                                               <  2:103906/150‑1 (MQ=255)
                                       gtggtgATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCCCCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGat                                                                                                              >  2:3761721/1‑150 (MQ=255)
                                        tggtgATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGatt                                                                                                             <  1:56982/150‑1 (MQ=255)
                                        tggtgATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGatt                                                                                                             <  1:1318598/150‑1 (MQ=255)
                                           tgATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTACGCGGGAGATGCTGCGCACCATAAATCCCACTACGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGaaacat                                                                                                          >  2:755288/1‑145 (MQ=255)
                                            gATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGattcatt                                                                                                         <  1:4120698/150‑1 (MQ=255)
                                             aTGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGattcattc                                                                                                        <  2:3121/150‑1 (MQ=255)
                                             aTGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGattcattc                                                                                                        <  2:1934323/150‑1 (MQ=255)
                                                 gcACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGc                                                                                                    >  2:2908225/1‑150 (MQ=255)
                                                 gcACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGc                                                                                                    >  2:3174874/1‑150 (MQ=255)
                                                 gcACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGc                                                                                                    <  1:2344960/150‑1 (MQ=255)
                                                 gcACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGc                                                                                                    <  1:2344993/150‑1 (MQ=255)
                                                  cACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCg                                                                                                   <  1:1281223/150‑1 (MQ=255)
                                                     cGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGAt                                                                                                <  1:1057342/150‑1 (MQ=255)
                                                      ggCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATc                                                                                               <  2:1712942/150‑1 (MQ=255)
                                                      ggCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATc                                                                                               <  2:1712862/150‑1 (MQ=255)
                                                          gCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCa                                                                                           >  2:4130078/1‑150 (MQ=255)
                                                           cAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCAc                                                                                          <  1:3680700/150‑1 (MQ=255)
                                                            aGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCAcc                                                                                         <  1:3235880/150‑1 (MQ=255)
                                                            aGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCAcc                                                                                         <  1:4342036/150‑1 (MQ=255)
                                                            aGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCAcc                                                                                         <  1:552703/150‑1 (MQ=255)
                                                                   gggCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATccct                                                                                  >  1:4371769/1‑149 (MQ=255)
                                                                   gggCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCa                                                                                  <  2:2179186/150‑1 (MQ=255)
                                                                     gCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGa                                                                                >  1:3248676/1‑150 (MQ=255)
                                                                       aGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGAcc                                                                              <  1:1932090/150‑1 (MQ=255)
                                                                          cATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATc                                                                           <  1:807780/150‑1 (MQ=255)
                                                                          cATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATc                                                                           <  1:4430851/150‑1 (MQ=255)
                                                                          cATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATc                                                                           <  1:806799/150‑1 (MQ=255)
                                                                           aTGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCa                                                                          >  1:4525867/1‑150 (MQ=255)
                                                                           aTGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCa                                                                          >  2:4175315/1‑150 (MQ=255)
                                                                                tACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGTACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCg                                                                     <  2:1449536/150‑1 (MQ=255)
                                                                                 aCGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGc                                                                    <  1:2758414/150‑1 (MQ=255)
                                                                                 aCGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGc                                                                    >  1:2824063/1‑150 (MQ=255)
                                                                                         aaCCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCttt                                                            >  2:2302784/1‑150 (MQ=255)
                                                                                          aCCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCtttt                                                           <  1:2659843/150‑1 (MQ=255)
                                                                                          aCCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCtttt                                                           >  2:1538430/1‑150 (MQ=255)
                                                                                           ccGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCttttt                                                          >  1:1233802/1‑150 (MQ=255)
                                                                                           ccGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCttttt                                                          <  1:3851761/150‑1 (MQ=255)
                                                                                             ggCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGc                                                        >  1:3737099/1‑150 (MQ=255)
                                                                                              gcagcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCa                                                       <  2:3908087/150‑1 (MQ=255)
                                                                                                agcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAgg                                                     >  1:1455386/1‑150 (MQ=255)
                                                                                                 gcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGc                                                    >  2:4312052/1‑150 (MQ=255)
                                                                                                     aTCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATc                                                <  2:198188/150‑1 (MQ=255)
                                                                                                      tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                               <  2:990833/150‑1 (MQ=255)
                                                                                                      tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                               <  1:4130554/150‑1 (MQ=255)
                                                                                                      tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                               >  1:2238525/1‑150 (MQ=255)
                                                                                                      tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                               <  1:656085/150‑1 (MQ=255)
                                                                                                      tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                               <  1:1623712/150‑1 (MQ=255)
                                                                                                       cAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCg                                              >  2:2628608/1‑150 (MQ=255)
                                                                                                        aaTCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGc                                             >  1:3325826/1‑150 (MQ=255)
                                                                                                         aTCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGct                                            >  1:3519796/1‑150 (MQ=255)
                                                                                                           cGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttc                                          >  1:2590102/1‑150 (MQ=255)
                                                                                                           cGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttc                                          >  1:291200/1‑150 (MQ=255)
                                                                                                            gCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttct                                         >  1:1550675/1‑150 (MQ=255)
                                                                                                             ccccTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttctt                                        >  2:1877076/1‑150 (MQ=255)
                                                                                                                cTTCGCGGGAGATGCTGCGCACCATACATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCca                                     <  1:3909882/150‑1 (MQ=255)
                                                                                                                cTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCca                                     <  1:2477902/150‑1 (MQ=255)
                                                                                                                  tCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCcagc                                   >  2:3323211/1‑150 (MQ=255)
                                                                                                                   cgcgGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCcagca                                  >  2:2684260/1‑150 (MQ=255)
                                                                                                                     cgGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAgg                                >  2:2462632/1‑150 (MQ=255)
                                                                                                                      gggAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGa                               >  1:410172/1‑150 (MQ=255)
                                                                                                                          gaTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTg                           <  2:1754427/150‑1 (MQ=255)
                                                                                                                           aTGCTGCGCACCATAACTCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGc                          <  2:3833040/150‑1 (MQ=255)
                                                                                                                           aTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGc                          <  2:4107213/150‑1 (MQ=255)
                                                                                                                           aTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGc                          <  2:1334083/150‑1 (MQ=255)
                                                                                                                               tgcGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCa                      >  1:3878398/1‑150 (MQ=255)
                                                                                                                                 cgcACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACt                    <  2:578827/150‑1 (MQ=255)
                                                                                                                                 cgcACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACt                    >  1:1457917/1‑150 (MQ=255)
                                                                                                                                 cgcACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACt                    >  2:4531082/1‑150 (MQ=255)
                                                                                                                                       aTAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGt              >  1:3733574/1‑150 (MQ=255)
                                                                                                                                       aTAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGt              >  2:718097/1‑150 (MQ=255)
                                                                                                                                       aTAAATCCCACTTCGTCCATATGCACACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGt              >  1:420278/1‑150 (MQ=255)
                                                                                                                                          aaTCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTcc           <  1:2531212/150‑1 (MQ=255)
                                                                                                                                            tCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCga         >  1:3405973/1‑150 (MQ=255)
                                                                                                                                              ccACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCgagg       >  1:4156228/1‑150 (MQ=255)
                                                                                                                                               cACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCgagga      >  1:2980160/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCG  >  CP009273/2498686‑2498982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑