Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,498,906 C→A 7.7% G41C (GGT→TGT)  ypdE ← aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,498,9060CA 7.7% 1050.8 / 41.0 283G41C (GGT→TGT) ypdEaminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (130/131);  new base A (14/8);  total (144/139)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 287 total aligned reads displayed.

AGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTT  >  CP009273/2498757‑2499054
                                                                                                                                                     |                                                                                                                                                    
aGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGAcc                                                                                                                                                      <  1:1932090/150‑1 (MQ=255)
   cATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATc                                                                                                                                                   <  1:806881/150‑1 (MQ=255)
    aTGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCa                                                                                                                                                  >  1:4525867/1‑150 (MQ=255)
        gTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATc                                                                                                                                              >  1:3635285/1‑150 (MQ=255)
          aCGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGc                                                                                                                                            <  1:2758304/150‑1 (MQ=255)
             ttGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCAct                                                                                                                                         <  2:4046985/150‑1 (MQ=255)
                ccAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCActtct                                                                                                                                      >  2:1513814/1‑150 (MQ=255)
                 cAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCActtctt                                                                                                                                     <  1:4278822/150‑1 (MQ=255)
                   aCCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCtttt                                                                                                                                   <  1:2659688/150‑1 (MQ=255)
                    ccGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCttttt                                                                                                                                  >  1:3505826/1‑150 (MQ=255)
                    ccGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCttttt                                                                                                                                  <  1:3851761/150‑1 (MQ=255)
                    ccGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCttttt                                                                                                                                  >  1:1233802/1‑150 (MQ=255)
                      ggCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGc                                                                                                                                >  1:3736701/1‑150 (MQ=255)
                       gcagcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCa                                                                                                                               <  1:1612585/150‑1 (MQ=255)
                       gcagcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCa                                                                                                                               <  2:3908087/150‑1 (MQ=255)
                         agcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAgg                                                                                                                             >  2:2293667/1‑150 (MQ=255)
                         agcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAgg                                                                                                                             >  1:1455386/1‑150 (MQ=255)
                          gcaCATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGc                                                                                                                            >  2:4312052/1‑150 (MQ=255)
                            acaTCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGa                                                                                                                          >  2:805629/1‑150 (MQ=255)
                               tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                                                                                                       <  1:4130554/150‑1 (MQ=255)
                               tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                                                                                                       <  2:990632/150‑1 (MQ=255)
                               tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                                                                                                       <  1:656085/150‑1 (MQ=255)
                               tCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATcc                                                                                                                       <  1:1623712/150‑1 (MQ=255)
                                cAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCg                                                                                                                      >  2:2628608/1‑150 (MQ=255)
                                    cGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttc                                                                                                                  >  1:2590102/1‑150 (MQ=255)
                                     gCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttct                                                                                                                 <  2:46820/150‑1 (MQ=255)
                                      ccccTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGcttctt                                                                                                                >  2:1877076/1‑150 (MQ=255)
                                         cTTCGCGGGAGATGCTGCGCACCATACATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCca                                                                                                             <  1:3909882/150‑1 (MQ=255)
                                         cTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCca                                                                                                             <  1:2477902/150‑1 (MQ=255)
                                            cgcgGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCcagca                                                                                                          >  2:2684297/1‑150 (MQ=255)
                                              cgGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAgg                                                                                                        >  2:2462632/1‑150 (MQ=255)
                                               gggAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGa                                                                                                       >  1:410172/1‑150 (MQ=255)
                                                 gagaTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATc                                                                                                     >  2:3288815/1‑150 (MQ=255)
                                                  agaTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCt                                                                                                    <  2:4490598/150‑1 (MQ=255)
                                                   gaTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTg                                                                                                   <  2:1754427/150‑1 (MQ=255)
                                                    aTGCTGCGCACCATAACTCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGc                                                                                                  <  2:3833040/150‑1 (MQ=255)
                                                    aTGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGc                                                                                                  <  2:4107213/150‑1 (MQ=255)
                                                     tgctgcGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGcc                                                                                                 >  1:3326114/1‑150 (MQ=255)
                                                     tgctgcGCACCATAAATCCCACTTAGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGcc                                                                                                 >  1:3264565/1‑150 (MQ=255)
                                                        tgcGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCa                                                                                              >  1:3878398/1‑150 (MQ=255)
                                                                aTAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGt                                                                                      >  2:718097/1‑150 (MQ=255)
                                                                aTAAATCCCACTTCGTCCATATGCACACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGt                                                                                      >  1:420278/1‑150 (MQ=255)
                                                                   aaTCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTcc                                                                                   <  1:2531211/150‑1 (MQ=255)
                                                                    aTCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCg                                                                                  >  1:2228598/1‑150 (MQ=255)
                                                                    aTCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCg                                                                                  >  2:2050289/1‑150 (MQ=255)
                                                                     tCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCga                                                                                 >  1:3405973/1‑150 (MQ=255)
                                                                        cACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCgagga                                                                              >  1:2980160/1‑150 (MQ=255)
                                                                         aCTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCgaggag                                                                             <  1:2805726/150‑1 (MQ=255)
                                                                               tCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATc                                                                       >  1:4193621/1‑150 (MQ=255)
                                                                               tCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATc                                                                       >  1:3245113/1‑150 (MQ=255)
                                                                                  atatGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCa                                                                    >  1:512091/1‑150 (MQ=255)
                                                                                   tatGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCAt                                                                   <  1:719051/150‑1 (MQ=255)
                                                                                    atGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATc                                                                  >  1:1128004/1‑150 (MQ=255)
                                                                                    atGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATc                                                                  >  2:1290400/1‑150 (MQ=255)
                                                                                     tGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCt                                                                 <  2:3635285/150‑1 (MQ=255)
                                                                                     tGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCt                                                                 <  2:1368874/150‑1 (MQ=255)
                                                                                      gcgcACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGACGCACTTCCTGTTCCGAGGAGGCGATCGCATCTg                                                                <  2:2033636/150‑1 (MQ=255)
                                                                                       cgcACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGc                                                               >  2:2236127/1‑150 (MQ=255)
                                                                                        gcACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGcc                                                              <  1:3328936/150‑1 (MQ=255)
                                                                                           caGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCg                                                           <  1:979299/150‑1 (MQ=255)
                                                                                            aGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGc                                                          >  2:4150374/1‑150 (MQ=255)
                                                                                            aGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGc                                                          >  1:872461/1‑150 (MQ=255)
                                                                                                  tcaCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACg                                                    <  2:272433/150‑1 (MQ=255)
                                                                                                    aCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCt                                                  >  2:1665155/1‑150 (MQ=255)
                                                                                                    aCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCt                                                  >  1:4473930/1‑150 (MQ=255)
                                                                                                     ccTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCtt                                                 <  2:593697/150‑1 (MQ=255)
                                                                                                        tCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTa                                              <  2:2868294/150‑1 (MQ=255)
                                                                                                          ggACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAAt                                            <  2:2943241/150‑1 (MQ=255)
                                                                                                          ggACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAAt                                            >  1:3125861/1‑150 (MQ=255)
                                                                                                          ggACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAAt                                            <  2:689926/150‑1 (MQ=255)
                                                                                                              cTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCg                                        >  1:2901922/1‑150 (MQ=255)
                                                                                                              cTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTGTTAATAGCg                                        >  1:2323440/1‑150 (MQ=255)
                                                                                                               tGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGa                                       >  1:2333522/1‑150 (MQ=255)
                                                                                                               tGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGa                                       <  2:159878/150‑1 (MQ=255)
                                                                                                                    attcattcAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAAt                                  <  2:4517720/150‑1 (MQ=255)
                                                                                                                     ttcattcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATc                                 <  2:2115198/150‑1 (MQ=255)
                                                                                                                       cattcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCa                               <  2:1033884/150‑1 (MQ=255)
                                                                                                                        attcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCAt                              <  1:1513814/150‑1 (MQ=255)
                                                                                                                          tcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTa                            >  2:2327991/1‑150 (MQ=255)
                                                                                                                          tcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTa                            <  1:4022958/150‑1 (MQ=255)
                                                                                                                           caGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTAt                           >  2:2665212/1‑150 (MQ=255)
                                                                                                                           caGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTAt                           <  1:2258983/150‑1 (MQ=255)
                                                                                                                            aGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGAAAAGCCGAACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATg                          >  1:3699917/1‑150 (MQ=255)
                                                                                                                              gCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGcc                        <  2:4139208/150‑1 (MQ=255)
                                                                                                                               cGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCct                       >  2:2443697/1‑150 (MQ=255)
                                                                                                                                  aTCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCtctc                    >  1:1305062/1‑150 (MQ=255)
                                                                                                                                    cAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTccc                  <  1:1084148/150‑1 (MQ=255)
                                                                                                                                    cAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTccc                  <  1:4376285/150‑1 (MQ=255)
                                                                                                                                    cAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTccc                  <  1:414277/150‑1 (MQ=255)
                                                                                                                                    cAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTccc                  <  1:4150374/150‑1 (MQ=255)
                                                                                                                                    cAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTccc                  <  1:4375379/150‑1 (MQ=255)
                                                                                                                                     aGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGGTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCg                 <  2:4477128/150‑1 (MQ=255)
                                                                                                                                      gCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGt                >  2:4214026/1‑150 (MQ=255)
                                                                                                                                       cACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCAGTATGCCTCTCCCGTg               >  2:4350780/1‑150 (MQ=255)
                                                                                                                                            aTCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCaa          <  1:1806069/150‑1 (MQ=255)
                                                                                                                                               ccAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAacac       >  1:2924079/1‑150 (MQ=255)
                                                                                                                                                cAGACAATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACt      >  1:1009597/1‑150 (MQ=255)
                                                                                                                                                  gACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGt    <  1:2684297/150‑1 (MQ=255)
                                                                                                                                                    ccATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGttt  >  2:3161109/1‑150 (MQ=255)
                                                                                                                                                    ccATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCAGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGttt  >  2:1352032/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTT  >  CP009273/2498757‑2499054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑