Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,125 G→T 6.9% G218C (GGT→TGT)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,1250GT 6.9% 859.4 / 25.4 231G218C (GGT→TGT) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (98/117);  new base T (5/11);  total (103/128)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 234 total aligned reads displayed.

TCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGG  >  CP009273/2618982‑2619274
                                                                                                                                               |                                                                                                                                                     
  aGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATa                                                                                                                                               >  2:2398424/1‑150 (MQ=255)
     tttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaaa                                                                                                                                            >  1:4007412/1‑150 (MQ=255)
     tttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaaa                                                                                                                                            <  2:2727630/150‑1 (MQ=255)
        gCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgcc                                                                                                                                         <  2:4365406/150‑1 (MQ=255)
        gCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgcc                                                                                                                                         <  2:2957164/150‑1 (MQ=255)
         cccAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccg                                                                                                                                        >  1:894750/1‑150 (MQ=255)
            aGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGccc                                                                                                                                     >  1:3957909/1‑150 (MQ=255)
            aGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGccc                                                                                                                                     >  1:1684203/1‑150 (MQ=255)
                ttATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGa                                                                                                                                 >  1:1024676/1‑150 (MQ=255)
                   ttttCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAgt                                                                                                                              <  1:2249215/150‑1 (MQ=255)
                   ttttCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAgt                                                                                                                              >  1:953017/1‑150 (MQ=255)
                    tttCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAgtg                                                                                                                             <  1:3315790/150‑1 (MQ=255)
                       ccTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTa                                                                                                                          >  2:3809243/1‑150 (MQ=255)
                          ggCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATg                                                                                                                       >  1:1589224/1‑150 (MQ=255)
                          ggCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATg                                                                                                                       >  1:1978974/1‑150 (MQ=255)
                           gCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATgg                                                                                                                      <  1:4385742/150‑1 (MQ=255)
                           gCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATgg                                                                                                                      <  2:3516482/150‑1 (MQ=255)
                             ggggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGaa                                                                                                                    <  2:4469325/150‑1 (MQ=255)
                              gggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGaaa                                                                                                                   >  1:2981304/1‑150 (MQ=255)
                               ggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAAt                                                                                                                  >  2:4224420/1‑150 (MQ=255)
                                  catcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATggg                                                                                                               <  1:4193099/150‑1 (MQ=255)
                                   atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGc                                                                                                              >  1:1367593/1‑150 (MQ=255)
                                   atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGc                                                                                                              <  2:2569885/150‑1 (MQ=255)
                                     caATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTATTGGAAATGGGCga                                                                                                            >  2:3677938/1‑150 (MQ=255)
                                     caATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCga                                                                                                            <  1:1118754/150‑1 (MQ=255)
                                      aaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgag                                                                                                           >  1:3053047/1‑150 (MQ=255)
                                       aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                          <  1:1932287/150‑1 (MQ=255)
                                       aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                          >  2:3330024/1‑150 (MQ=255)
                                         aaaGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaaa                                                                                                        >  1:3856307/1‑150 (MQ=255)
                                         aaaGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGaaa                                                                                                        <  1:3818103/150‑1 (MQ=255)
                                            gagaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGAc                                                                                                     <  1:1046361/150‑1 (MQ=255)
                                             agaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACg                                                                                                    <  2:1312645/150‑1 (MQ=255)
                                             agaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACg                                                                                                    >  1:2686852/1‑150 (MQ=255)
                                               aaTTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACggg                                                                                                  >  1:2695060/1‑150 (MQ=255)
                                                aTTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGa                                                                                                 >  1:3365187/1‑150 (MQ=255)
                                                   ttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATaa                                                                                              <  1:323532/150‑1 (MQ=255)
                                                   ttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATaa                                                                                              >  1:3568636/1‑150 (MQ=255)
                                                     cAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAAtt                                                                                            >  2:1318910/1‑150 (MQ=255)
                                                       tctcgcTAGCATGTCGGCATCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAc                                                                                          <  1:3737743/147‑1 (MQ=255)
                                                       gcgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAAGTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAc                                                                                          <  1:55230/150‑1 (MQ=255)
                                                         gcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCAGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAccc                                                                                        >  2:2890231/1‑150 (MQ=255)
                                                          cgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAcccc                                                                                       <  1:3901328/150‑1 (MQ=255)
                                                          cgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAcccc                                                                                       <  1:3901299/150‑1 (MQ=255)
                                                          cgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAcccc                                                                                       <  1:144872/150‑1 (MQ=255)
                                                           gcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                                      <  1:1409744/150‑1 (MQ=255)
                                                                 aTGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGt                                                                                >  2:4122391/1‑150 (MQ=255)
                                                                  tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTc                                                                               <  2:2693677/150‑1 (MQ=255)
                                                                   ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                              <  1:2090373/150‑1 (MQ=255)
                                                                   ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCt                                                                              <  2:3779210/150‑1 (MQ=255)
                                                                     cggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTgg                                                                            <  2:844212/150‑1 (MQ=255)
                                                                      ggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGa                                                                           >  1:252960/1‑150 (MQ=255)
                                                                      ggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGa                                                                           >  1:3177997/1‑150 (MQ=255)
                                                                      ggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGa                                                                           <  2:190026/150‑1 (MQ=255)
                                                                           cacaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAAc                                                                      <  2:3023014/150‑1 (MQ=255)
                                                                            acaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACt                                                                     <  2:1010303/150‑1 (MQ=255)
                                                                            acaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACt                                                                     >  2:271763/1‑150 (MQ=255)
                                                                             caAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTg                                                                    <  2:2668055/150‑1 (MQ=255)
                                                                             caAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTg                                                                    <  2:2912693/150‑1 (MQ=255)
                                                                              aaaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTgg                                                                   >  2:4194969/1‑150 (MQ=255)
                                                                               aaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGt                                                                  >  2:1665420/1‑150 (MQ=255)
                                                                                   cTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaaaa                                                              <  2:1451444/150‑1 (MQ=255)
                                                                                     ggAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaaga                                                            <  1:1240711/150‑1 (MQ=255)
                                                                                       aaaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaagaag                                                          >  1:3448871/1‑150 (MQ=255)
                                                                                        aaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGt                                                         <  2:4386879/150‑1 (MQ=255)
                                                                                         aCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGtt                                                        >  2:1956019/1‑150 (MQ=255)
                                                                                          cTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGttt                                                       <  2:1278558/150‑1 (MQ=255)
                                                                                          cTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGttt                                                       <  1:2627103/150‑1 (MQ=255)
                                                                                             tAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAc                                                    <  1:3949102/150‑1 (MQ=255)
                                                                                             tAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAc                                                    <  2:3417465/150‑1 (MQ=255)
                                                                                             tAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTAc                                                    >  1:1545620/1‑150 (MQ=255)
                                                                                              aaCCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACg                                                   <  1:866876/150‑1 (MQ=255)
                                                                                                ccTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTc                                                 >  1:586246/1‑150 (MQ=255)
                                                                                                   ggCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCAcc                                              >  2:2845683/1‑150 (MQ=255)
                                                                                                    gCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTTAAAGAAGTTTTACGTCACCg                                             <  2:320107/150‑1 (MQ=255)
                                                                                                    gCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCg                                             <  2:2614872/150‑1 (MQ=255)
                                                                                                     cAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAAGGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGt                                            >  2:124727/1‑150 (MQ=255)
                                                                                                     cAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGt                                            >  1:1718934/1‑150 (MQ=255)
                                                                                                       aTTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTaa                                          >  2:1604666/1‑150 (MQ=255)
                                                                                                          ccGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAttt                                       <  1:1845883/150‑1 (MQ=255)
                                                                                                           cGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                                      >  1:2975910/1‑150 (MQ=255)
                                                                                                           cGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                                      >  1:3788139/1‑150 (MQ=255)
                                                                                                                tCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATc                                 <  1:4192327/150‑1 (MQ=255)
                                                                                                                 cAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCg                                >  2:2439811/1‑150 (MQ=255)
                                                                                                                    ggCTGGATCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTg                             <  2:2330938/150‑1 (MQ=255)
                                                                                                                          aaCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTa                       >  1:2112157/1‑150 (MQ=255)
                                                                                                                               tGCACTGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCggg                  <  2:3494443/150‑1 (MQ=255)
                                                                                                                               tGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCggg                  <  1:1723014/150‑1 (MQ=255)
                                                                                                                                gCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGt                 >  2:859934/1‑150 (MQ=255)
                                                                                                                                gCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGAGt                 <  2:3824928/150‑1 (MQ=255)
                                                                                                                                gCAAGGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGAGt                 <  2:3825010/150‑1 (MQ=255)
                                                                                                                                 cAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTc                <  2:3856226/150‑1 (MQ=255)
                                                                                                                                   aTGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCtt              <  2:2686852/150‑1 (MQ=255)
                                                                                                                                     gggCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTc            <  1:3157053/150‑1 (MQ=255)
                                                                                                                                       gcgcTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCg          <  2:2286104/150‑1 (MQ=255)
                                                                                                                                           ttCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAaga      >  2:4073631/1‑150 (MQ=255)
                                                                                                                                            tCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAagag     <  2:1589174/150‑1 (MQ=255)
                                                                                                                                            tCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAagag     <  2:1595392/150‑1 (MQ=255)
                                                                                                                                            tCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAagag     <  2:1978974/150‑1 (MQ=255)
                                                                                                                                               ggTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCgg  <  2:4525898/150‑1 (MQ=255)
                                                                                                                                               ggTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCgg  <  2:4526341/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
TCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTCCGAAGAGCGG  >  CP009273/2618982‑2619274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑