Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,876,760 C→T 93.3% G246G (GGG→GGA)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,876,7600CT93.3% 987.8 / 43.9 297G246G (GGG→GGA) casACRISP RNA (crRNA) containing Cascade antiviral complex protein
Reads supporting (aligned to +/- strand):  ref base C (5/15);  new base T (128/149);  total (133/164)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 298 total aligned reads displayed.

TTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAATT  >  CP009273/2876613‑2876905
                                                                                                                                                   |                                                                                                                                                 
 tCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCa                                                                                                                                                <  1:2793395/150‑1 (MQ=255)
   cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAATCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAAt                                                                                                                                              <  2:840975/150‑1 (MQ=255)
   cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAAt                                                                                                                                              >  2:1284487/1‑150 (MQ=255)
    tCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATg                                                                                                                                             <  1:1432389/150‑1 (MQ=255)
     cAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATgg                                                                                                                                            >  2:2230416/1‑150 (MQ=255)
     cAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCATGTCCACAGCAAGAACATTTACCAATTCCAATgg                                                                                                                                            >  2:2911326/1‑150 (MQ=255)
      aaCCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATggg                                                                                                                                           >  1:94013/1‑150 (MQ=255)
      aaCCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATggg                                                                                                                                           >  2:624810/1‑150 (MQ=255)
         cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATc                                                                                                                                        <  1:398981/150‑1 (MQ=255)
         cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATc                                                                                                                                        <  2:3808907/150‑1 (MQ=255)
         cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATc                                                                                                                                        <  2:3810522/150‑1 (MQ=255)
           ccccTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGc                                                                                                                                      >  2:2677403/1‑150 (MQ=255)
              cTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATa                                                                                                                                   >  2:3078970/1‑150 (MQ=255)
                ttCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAt                                                                                                                                 >  1:3422645/1‑150 (MQ=255)
                     gACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAat                                                                                                                            >  1:3552435/1‑150 (MQ=255)
                       cTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAatat                                                                                                                          <  2:1671651/150‑1 (MQ=255)
                          ttACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCATAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcg                                                                                                                       >  1:2410234/1‑150 (MQ=255)
                           tACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                      >  1:1485314/1‑150 (MQ=255)
                           tACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                      >  1:4515882/1‑150 (MQ=255)
                            aCCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCt                                                                                                                     <  1:4036877/150‑1 (MQ=255)
                             ccAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTg                                                                                                                    <  2:3997775/150‑1 (MQ=255)
                               aGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                  >  1:2036642/1‑150 (MQ=255)
                               aGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                  <  1:2017356/150‑1 (MQ=255)
                                gACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGtt                                                                                                                 <  1:2141670/150‑1 (MQ=255)
                                gACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGtt                                                                                                                 <  1:1614556/150‑1 (MQ=255)
                                 aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTg                                                                                                                <  1:2023829/150‑1 (MQ=255)
                                  cAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGc                                                                                                               >  2:313225/1‑150 (MQ=255)
                                   aaGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCATAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGcc                                                                                                              <  2:2408768/150‑1 (MQ=255)
                                    aGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCa                                                                                                             >  1:475425/1‑150 (MQ=255)
                                    aGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCa                                                                                                             <  2:3323605/150‑1 (MQ=255)
                                      gggAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGa                                                                                                           <  2:1716095/150‑1 (MQ=255)
                                        gAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAAt                                                                                                         >  1:3723399/1‑150 (MQ=255)
                                            gCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGAc                                                                                                     >  1:582958/1‑150 (MQ=255)
                                            gCGGATGGGGCCATAGCCCATTAACTGTAAACGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGAc                                                                                                     <  2:4485925/150‑1 (MQ=255)
                                              ggATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCa                                                                                                   <  2:562139/150‑1 (MQ=255)
                                              ggATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCa                                                                                                   >  2:3544916/1‑150 (MQ=255)
                                                aTGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACg                                                                                                 <  1:3485247/150‑1 (MQ=255)
                                                aTGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACg                                                                                                 >  2:202888/1‑150 (MQ=255)
                                                aTGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACg                                                                                                 >  2:2747871/1‑150 (MQ=255)
                                                   gggCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGAc                                                                                              <  1:3748060/150‑1 (MQ=255)
                                                       cATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAc                                                                                          <  2:3845903/150‑1 (MQ=255)
                                                       cATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAc                                                                                          <  2:445596/150‑1 (MQ=255)
                                                        aTAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAcc                                                                                         <  1:987753/150‑1 (MQ=255)
                                                        aTAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAcc                                                                                         >  2:4138713/1‑150 (MQ=255)
                                                         tAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAccc                                                                                        <  1:4225366/150‑1 (MQ=255)
                                                             ccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAtt                                                                                    <  1:2698810/150‑1 (MQ=255)
                                                              cATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTg                                                                                   >  1:3228844/1‑150 (MQ=255)
                                                                ttAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGAc                                                                                 >  1:4250762/1‑150 (MQ=255)
                                                                 tAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACg                                                                                >  1:2918156/1‑150 (MQ=255)
                                                                 tAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACg                                                                                >  2:3043679/1‑150 (MQ=255)
                                                                   aCTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGaa                                                                              <  2:3427418/150‑1 (MQ=255)
                                                                    cTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAg                                                                             <  2:1081474/150‑1 (MQ=255)
                                                                        aaaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAgg                                                                         <  2:3686541/150‑1 (MQ=255)
                                                                          aGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGta                                                                       >  2:2593351/1‑150 (MQ=255)
                                                                           ggTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACACACCCAATTGACGAAGCAGGtat                                                                      <  2:1313190/150‑1 (MQ=255)
                                                                             tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGAcc                                                                                                    >  1:1760760/1‑118 (MQ=255)
                                                                             tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGAcc                                                                                                    <  2:1760760/118‑1 (MQ=255)
                                                                                 tttttCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTc                                                                >  1:2745394/1‑150 (MQ=255)
                                                                                  ttttCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCa                                                               <  1:255812/150‑1 (MQ=255)
                                                                                     tCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTg                                                            <  1:978198/150‑1 (MQ=255)
                                                                                      ccTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTgg                                                           <  1:624810/150‑1 (MQ=255)
                                                                                         tAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACt                                                        <  2:2036645/150‑1 (MQ=255)
                                                                                          aaGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACtt                                                       >  1:4384578/1‑150 (MQ=255)
                                                                                              aaaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATa                                                   <  1:916458/150‑1 (MQ=255)
                                                                                              aaaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATa                                                   >  1:2988139/1‑150 (MQ=255)
                                                                                                 ccGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGt                                                <  1:1464550/150‑1 (MQ=255)
                                                                                                  cGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGtt                                               <  1:3900241/150‑1 (MQ=255)
                                                                                                  cGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGtt                                               >  1:2495552/1‑150 (MQ=255)
                                                                                                     tataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            <  2:2478608/150‑1 (MQ=255)
                                                                                                     tataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTaa                                            >  1:107293/1‑150 (MQ=255)
                                                                                                      ataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAAt                                           <  2:1707716/150‑1 (MQ=255)
                                                                                                       taACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATc                                          >  2:849973/1‑150 (MQ=255)
                                                                                                         aCGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATTGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTATTCCa                                        <  2:4155520/150‑1 (MQ=255)
                                                                                                         aCGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCa                                        <  2:1901217/150‑1 (MQ=255)
                                                                                                          cGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCag                                       <  1:1673022/150‑1 (MQ=255)
                                                                                                           gCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCagg                                      >  2:1409493/1‑150 (MQ=255)
                                                                                                              aaTTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtag                                   >  1:2506195/1‑150 (MQ=255)
                                                                                                              aaTTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtag                                   >  1:1910853/1‑150 (MQ=255)
                                                                                                                 tGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggtt                                >  1:444699/1‑150 (MQ=255)
                                                                                                                 tGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggtt                                <  2:3893637/150‑1 (MQ=255)
                                                                                                                   cTTTCCTGTCCACAGCAAGAACATTTACCAATTCAAATGGGATAGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggttgg                              <  1:3669751/150‑1 (MQ=255)
                                                                                                                    tttCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggttggt                             >  1:2388722/1‑150 (MQ=255)
                                                                                                                      tCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           >  1:1002095/1‑150 (MQ=255)
                                                                                                                      tCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGttt                           >  2:3784877/1‑150 (MQ=255)
                                                                                                                         tGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTcc                        >  2:196425/1‑150 (MQ=255)
                                                                                                                         tGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTcc                        >  2:196879/1‑150 (MQ=255)
                                                                                                                             cacaGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAt                    <  1:3433653/150‑1 (MQ=255)
                                                                                                                               caGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgt                  >  2:2379214/1‑150 (MQ=255)
                                                                                                                               caGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgt                  <  1:3069790/150‑1 (MQ=255)
                                                                                                                                   aaGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGAtt              <  1:1304551/150‑1 (MQ=255)
                                                                                                                                    aGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc             <  2:1926139/150‑1 (MQ=255)
                                                                                                                                      aaCATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAt           <  2:230118/150‑1 (MQ=255)
                                                                                                                                      aaCATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAt           <  2:937067/150‑1 (MQ=255)
                                                                                                                                         aTTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTTACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAg        <  1:1364602/150‑1 (MQ=255)
                                                                                                                                         aTTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAg        >  1:3615518/1‑150 (MQ=255)
                                                                                                                                           ttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGa      <  1:3128787/150‑1 (MQ=255)
                                                                                                                                            tACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGaa     <  1:549569/150‑1 (MQ=255)
                                                                                                                                              ccAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAt   <  2:1485302/150‑1 (MQ=255)
                                                                                                                                               cAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAtt  <  1:2677403/150‑1 (MQ=255)
                                                                                                                                               cAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAtt  <  1:2285550/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTCCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAATT  >  CP009273/2876613‑2876905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑