Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,899,073 C→A 10.9% R2S (CGC→AGC)  ygcG → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,899,0730CA10.9% 961.3 / 65.0 293R2S (CGC→AGC) ygcGuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (116/145);  new base A (23/9);  total (139/154)
Fisher's exact test for biased strand distribution p-value = 4.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 295 total aligned reads displayed.

GAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAT  >  CP009273/2898925‑2899221
                                                                                                                                                    |                                                                                                                                                    
gaaAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATGAGAATTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGAGGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAAATTATAATgcg                                                                                                                                                     >  1:627643/1‑150 (MQ=255)
  aaaTAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCt                                                                                                                                                   >  2:958513/1‑150 (MQ=255)
  aaaTAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCt                                                                                                                                                   <  2:2873900/150‑1 (MQ=255)
    aTAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAt                                                                                                                                                 <  1:1010739/150‑1 (MQ=255)
    aTAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAt                                                                                                                                                 <  1:751441/150‑1 (MQ=255)
     tAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAtt                                                                                                                                                <  2:1136062/150‑1 (MQ=255)
     tAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAtt                                                                                                                                                >  1:2718189/1‑150 (MQ=255)
     tAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATATATCCAGATTTAATGAGAAATTTATAATGCGCTAtt                                                                                                                                                >  1:3680941/1‑150 (MQ=255)
      aaaGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAttt                                                                                                                                               >  2:1704570/1‑150 (MQ=255)
      aaaGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAttt                                                                                                                                               <  1:3139012/150‑1 (MQ=255)
      aaaGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTAttt                                                                                                                                               <  1:703363/150‑1 (MQ=255)
       aaGAAAATTGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTc                                                                                                                                              <  2:3555582/150‑1 (MQ=255)
       aaGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTc                                                                                                                                              <  1:3786973/150‑1 (MQ=255)
          aaaaTGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATa                                                                                                                                           <  1:3431213/150‑1 (MQ=255)
          aaaaTGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATa                                                                                                                                           >  2:1228952/1‑150 (MQ=255)
          aaaaTGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATa                                                                                                                                           <  1:1517381/150‑1 (MQ=255)
             aTGGATTTAATCATGTCAAAACATTCACCAGAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTa                                                                                                                                        >  2:2209529/1‑150 (MQ=255)
             aTGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTa                                                                                                                                        >  2:2041739/1‑150 (MQ=255)
                 aTTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGt                                                                                                                                    >  2:1502410/1‑150 (MQ=255)
                   ttAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTc                                                                                                                                  <  2:1873707/150‑1 (MQ=255)
                   ttAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTc                                                                                                                                  <  2:2033773/150‑1 (MQ=255)
                    tAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCa                                                                                                                                 >  1:240318/1‑150 (MQ=255)
                     aaTCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCAc                                                                                                                                <  1:4470181/150‑1 (MQ=255)
                      aTCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACt                                                                                                                               <  2:1637526/150‑1 (MQ=255)
                         aTGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTc                                                                                                                            <  1:2953434/150‑1 (MQ=255)
                         aTGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTc                                                                                                                            <  1:1882931/150‑1 (MQ=255)
                          tGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCg                                                                                                                           <  1:215713/150‑1 (MQ=255)
                           gTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGt                                                                                                                          >  1:10692/1‑150 (MQ=255)
                               aaaCATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGt                                                                                                                      <  2:2622589/150‑1 (MQ=255)
                                aaCATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGtt                                                                                                                     <  2:1564648/150‑1 (MQ=255)
                                   aTTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCt                                                                                                                  >  2:2269126/1‑150 (MQ=255)
                                    ttCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCtt                                                                                                                 <  2:2100698/150‑1 (MQ=255)
                                        ccAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTttgtt                                                                                                             <  2:30137/150‑1 (MQ=255)
                                         cAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTttgttg                                                                                                            <  2:34880/150‑1 (MQ=255)
                                         cAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTttgttg                                                                                                            <  2:2477580/150‑1 (MQ=255)
                                           aaaTTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGct                                                                                                          >  2:1665437/1‑149 (MQ=255)
                                              ttATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAAGATAAAATTACCGATGCCGCCGTCAATTTATATATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGcc                                                                                                       >  1:3689667/1‑150 (MQ=255)
                                               tataTTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGccc                                                                                                      >  2:3984235/1‑150 (MQ=255)
                                               tataTTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGccc                                                                                                      >  1:4035868/1‑150 (MQ=255)
                                               tataTTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGccc                                                                                                      <  1:437703/150‑1 (MQ=255)
                                                 tatttattTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCccaa                                                                                                    >  1:596234/1‑150 (MQ=255)
                                                  atttatttTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTCCCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTCATGTTCACTTGCGTATGTTCCTTTGTTGCAGCcctac                                                                                                   >  2:2750987/1‑150 (MQ=255)
                                                   tttatttTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCccaacc                                                                                                  >  2:2302607/1‑150 (MQ=255)
                                                    ttatttTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCccaacca                                                                                                 <  1:2892614/150‑1 (MQ=255)
                                                    ttatttTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGGccaacca                                                                                                 <  2:3667678/150‑1 (MQ=255)
                                                        tttttCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTa                                                                                             >  2:2738096/1‑150 (MQ=255)
                                                          tttCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTAtt                                                                                           >  1:1299656/1‑150 (MQ=255)
                                                          tttCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTAtt                                                                                           >  1:3570082/1‑150 (MQ=255)
                                                            tCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGt                                                                                         >  1:3902263/1‑150 (MQ=255)
                                                             cTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTc                                                                                        >  2:2051328/1‑150 (MQ=255)
                                                                    aGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAAt                                                                                 >  1:1930318/1‑150 (MQ=255)
                                                                    aGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAAt                                                                                 >  2:4454248/1‑150 (MQ=255)
                                                                       ttttAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTAc                                                                              >  2:2065172/1‑150 (MQ=255)
                                                                       ttttAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTAc                                                                              <  1:1705571/150‑1 (MQ=255)
                                                                        tttAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACa                                                                             <  2:2695712/150‑1 (MQ=255)
                                                                           aaTGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGca                                                                          >  1:3806296/1‑150 (MQ=255)
                                                                           aaTGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGca                                                                          >  2:3992062/1‑150 (MQ=255)
                                                                             tGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGcaac                                                                        >  1:425714/1‑150 (MQ=255)
                                                                              gATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGcaaca                                                                       >  1:3068407/1‑150 (MQ=255)
                                                                              gATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGcaaca                                                                       >  1:3069425/1‑150 (MQ=255)
                                                                              gATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGcaaca                                                                       >  2:1939279/1‑150 (MQ=255)
                                                                               aTAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGcaacaa                                                                      <  1:3181122/150‑1 (MQ=255)
                                                                                   cGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTa                                                                  >  2:3411151/1‑150 (MQ=255)
                                                                                    gAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTAc                                                                 >  2:2761538/1‑150 (MQ=255)
                                                                                     aaTATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACg                                                                >  2:625887/1‑150 (MQ=255)
                                                                                             aTTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTAc                                                        <  2:511521/150‑1 (MQ=255)
                                                                                                aCCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAg                                                     >  2:3294551/1‑150 (MQ=255)
                                                                                                 ccGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTCATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGt                                                    <  1:1800061/150‑1 (MQ=255)
                                                                                                 ccGATGCCGCCGTCAATTTATTTAACCAGAATTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGAAAGTTCCTTTGTTGCAGCCCAACCAAATATTGTCCCAAAATTACAGAAACAAGTTACGGATATTACGAga                                                    >  1:3730701/1‑149 (MQ=255)
                                                                                                  cGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTa                                                   >  1:786301/1‑150 (MQ=255)
                                                                                                     tGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCt                                                <  1:3112797/150‑1 (MQ=255)
                                                                                                      gccgccGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCtt                                               <  1:2247563/150‑1 (MQ=255)
                                                                                                       ccgccgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTa                                              >  1:1435638/1‑150 (MQ=255)
                                                                                                        cgccgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTaa                                             <  2:2196392/150‑1 (MQ=255)
                                                                                                             tCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTca                                        >  1:1219814/1‑150 (MQ=255)
                                                                                                              cAATTTATTTATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTcac                                       >  2:1258975/1‑150 (MQ=255)
                                                                                                               aaTTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTcaca                                      <  1:1376606/150‑1 (MQ=255)
                                                                                                                atttatttATCCAGATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaa                                     >  1:1645164/1‑150 (MQ=255)
                                                                                                                 tttatttaTCCATATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaag                                    <  2:3677036/150‑1 (MQ=255)
                                                                                                                 tttatttaTCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaag                                    <  2:416282/150‑1 (MQ=255)
                                                                                                                        atCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaagaaaagaa                             >  1:82424/1‑150 (MQ=255)
                                                                                                                            aGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAagaaagaa                         >  1:3479344/1‑150 (MQ=255)
                                                                                                                            aGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAagaaagaa                         >  2:4041344/1‑150 (MQ=255)
                                                                                                                             gATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAAc                        <  2:3794218/150‑1 (MQ=255)
                                                                                                                             gATTTAATGAGAAATTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAAc                        <  2:3902263/150‑1 (MQ=255)
                                                                                                                               tttAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTg                      <  2:1353078/150‑1 (MQ=255)
                                                                                                                               tttAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTg                      <  2:4133789/150‑1 (MQ=255)
                                                                                                                                 tAATGAGAAATTTATAATGAGATATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGAc                    <  1:3294551/150‑1 (MQ=255)
                                                                                                                                  aaTGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACg                   <  1:3788443/150‑1 (MQ=255)
                                                                                                                                     gagaAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGcac                <  1:1777677/150‑1 (MQ=255)
                                                                                                                                      agaAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGcaca               <  2:1474401/150‑1 (MQ=255)
                                                                                                                                       gaAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACaa              <  1:232263/150‑1 (MQ=255)
                                                                                                                                          aTTTATAATGAGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGtt           >  2:3544327/1‑150 (MQ=255)
                                                                                                                                           tttATAATGCGCTATTTCATACTAATTTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACATGTTa          <  2:1711471/150‑1 (MQ=255)
                                                                                                                                            ttATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAg         >  2:3876925/1‑150 (MQ=255)
                                                                                                                                              ataATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGaa       <  2:4064798/150‑1 (MQ=255)
                                                                                                                                              ataATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGaa       >  2:668023/1‑150 (MQ=255)
                                                                                                                                              ataATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGaa       <  1:897728/150‑1 (MQ=255)
                                                                                                                                                 aTGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCt    >  2:814583/1‑150 (MQ=255)
                                                                                                                                                   gcgcTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAt  <  2:3229267/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAT  >  CP009273/2898925‑2899221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑