Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,934,843 G→T 87.9% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,934,8430GT87.9% 662.4 / 89.4 257H50N (CAT→AAT) ygdDUPF0382 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (24/7);  new base T (109/117);  total (133/124)
Fisher's exact test for biased strand distribution p-value = 3.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 259 total aligned reads displayed.

ACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGG  >  CP009273/2934699‑2934992
                                                                                                                                                |                                                                                                                                                     
   aaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCt                                                                                                                                               <  1:1137965/150‑1 (MQ=255)
      gCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGt                                                                                                                                            >  1:923306/1‑150 (MQ=255)
            gggACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGa                                                                                                                                      >  2:1393764/1‑150 (MQ=255)
              gACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAgg                                                                                                                                    <  1:2248635/150‑1 (MQ=255)
              gACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAgg                                                                                                                                    >  2:790228/1‑150 (MQ=255)
                cAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCAGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGcc                                                                                                                                  >  2:2022613/1‑150 (MQ=255)
                 aGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCg                                                                                                                                 <  1:582601/150‑1 (MQ=255)
                      cAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAATCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTg                                                                                                                            >  2:1328152/1‑150 (MQ=255)
                           aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                       >  2:2562454/1‑150 (MQ=255)
                           aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                       >  2:3913190/1‑150 (MQ=255)
                           aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                       >  2:2840885/1‑150 (MQ=255)
                              aaaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGc                                                                                                                    >  1:3869230/1‑150 (MQ=255)
                                    tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                              <  2:2405117/150‑1 (MQ=255)
                                    tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                              <  2:2405999/150‑1 (MQ=255)
                                     gCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtctc                                                                                                             <  2:2601193/150‑1 (MQ=255)
                                        gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                          <  2:3236543/150‑1 (MQ=255)
                                         cTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACg                                                                                                         <  2:2275057/150‑1 (MQ=255)
                                         cTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACg                                                                                                         <  2:2576035/150‑1 (MQ=255)
                                           gAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGc                                                                                                       >  1:1766325/1‑150 (MQ=255)
                                              caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                    >  2:2992685/1‑150 (MQ=255)
                                              caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                    >  1:2366726/1‑150 (MQ=255)
                                              caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGAGACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                    <  2:2630973/150‑1 (MQ=255)
                                               aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCTTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                   >  1:1164119/1‑150 (MQ=255)
                                                acacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCa                                                                                                  <  1:2056192/150‑1 (MQ=255)
                                                acacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCa                                                                                                  <  1:4263790/150‑1 (MQ=255)
                                                acacCGTGCCTAACGCGAGGAAAACGCTACTACAGTAAAACCAGATACTGATGCGACGCTGCTTTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTAGAGGCCGGTCTTGATCCAGCCCATCTCAACGGCCCCCa                                                                                                  <  2:3971618/150‑1 (MQ=255)
                                                 cacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCAt                                                                                                 <  1:75195/150‑1 (MQ=255)
                                                 cacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCAt                                                                                                 <  1:73527/150‑1 (MQ=255)
                                                 cacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCAt                                                                                                 >  1:477356/1‑150 (MQ=255)
                                                  acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                                >  1:1014317/1‑150 (MQ=255)
                                                  acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                                >  2:1034636/1‑150 (MQ=255)
                                                  acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                                >  2:1034755/1‑150 (MQ=255)
                                                   ccGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATgg                                                                                               <  2:55010/150‑1 (MQ=255)
                                                    cGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGt                                                                                              <  2:2522598/150‑1 (MQ=255)
                                                        ccTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTa                                                                                          <  2:1570323/150‑1 (MQ=255)
                                                          tAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACt                                                                                        >  2:428565/1‑150 (MQ=255)
                                                             cgcgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATACGAGGCCGGTCTGGATCCAGCCCAGCTCAACGGCCCCCATGGTTTTACTTaa                                                                                     >  1:3677527/1‑150 (MQ=255)
                                                              gcgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAac                                                                                    <  1:278577/150‑1 (MQ=255)
                                                               cgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAaca                                                                                   >  2:300792/1‑150 (MQ=255)
                                                                gAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAacac                                                                                  >  2:4451904/1‑150 (MQ=255)
                                                                 aGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTCAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAAcaca                                                                                 <  2:3865930/150‑1 (MQ=255)
                                                                 aGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAAcaca                                                                                 <  2:3866185/150‑1 (MQ=255)
                                                                  ggAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACAt                                                                                <  1:4347117/150‑1 (MQ=255)
                                                                   gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                               <  2:414881/150‑1 (MQ=255)
                                                                   gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                               >  1:666706/1‑150 (MQ=255)
                                                                   gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                               <  2:414950/150‑1 (MQ=255)
                                                                   gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                               >  1:666492/1‑150 (MQ=255)
                                                                   gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCACCCATGGTTTTACTTAACACATg                                                                               <  2:3658281/150‑1 (MQ=255)
                                                                     aaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcg                                                                             <  2:2312637/150‑1 (MQ=255)
                                                                      aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                            >  1:581557/1‑150 (MQ=255)
                                                                      aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                            >  2:471321/1‑150 (MQ=255)
                                                                      aaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgc                                                                            <  2:578313/150‑1 (MQ=255)
                                                                       aCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgcg                                                                           <  1:1317096/150‑1 (MQ=255)
                                                                       aCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgcg                                                                           <  1:1317337/150‑1 (MQ=255)
                                                                            aCTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCaaa                                                                      >  1:36273/1‑150 (MQ=255)
                                                                              tCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAg                                                                    <  2:4019387/150‑1 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAATATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   >  1:1118975/1‑150 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   <  2:3498001/150‑1 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   <  2:2417129/150‑1 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   >  1:3391736/1‑150 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   <  2:1225912/150‑1 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   >  2:3019690/1‑150 (MQ=255)
                                                                               ccAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgc                                                                   <  2:3514986/150‑1 (MQ=255)
                                                                                 aGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgcgc                                                                 <  1:4428109/150‑1 (MQ=255)
                                                                                 aGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgcgc                                                                 >  2:95988/1‑150 (MQ=255)
                                                                                   tAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGccc                                                               >  2:177403/1‑150 (MQ=255)
                                                                                     aaaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCag                                                             <  2:1136667/150‑1 (MQ=255)
                                                                                     aaaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCag                                                             >  1:1025983/1‑150 (MQ=255)
                                                                                      aaCCAGATACTGATGCGTCGCTGCATTGCCTCCGCCAGACCTACGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTATGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCaga                                                            <  1:1507273/150‑1 (MQ=255)
                                                                                       aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                           <  2:3364278/150‑1 (MQ=255)
                                                                                       aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                           <  2:3364295/150‑1 (MQ=255)
                                                                                       aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                           <  2:117697/150‑1 (MQ=255)
                                                                                       aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                           <  2:1730128/150‑1 (MQ=255)
                                                                                        ccAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGc                                                          >  2:854979/1‑150 (MQ=255)
                                                                                        ccAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGc                                                          >  2:3790648/1‑150 (MQ=255)
                                                                                         cAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGcc                                                         >  1:2813881/1‑150 (MQ=255)
                                                                                          aGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCca                                                        >  1:4291694/1‑150 (MQ=255)
                                                                                           tctACTGATGAGTCTCTGCATTGCCTCCGCCAGACCTAAGTTCGCCAGCGTATTAAACGCCTGGTATTAGAGGCCGGTCTGGATCCTGCCCATCTCAACGTCCCCCTTGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcac                                                       <  2:3335786/148‑1 (MQ=255)
                                                                                           gATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcac                                                       >  1:2162538/1‑150 (MQ=255)
                                                                                           gATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcac                                                       >  1:3187271/1‑150 (MQ=255)
                                                                                            aTACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                      >  1:1960406/1‑150 (MQ=255)
                                                                                            aTACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                      >  1:1961063/1‑150 (MQ=255)
                                                                                                 gATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAAt                                                 >  2:562802/1‑150 (MQ=255)
                                                                                                  aTGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATg                                                <  1:1186507/150‑1 (MQ=255)
                                                                                                   tGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGa                                               <  1:2614647/150‑1 (MQ=255)
                                                                                                      gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                            >  1:4342121/1‑150 (MQ=255)
                                                                                                       aCGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGcc                                           <  2:2304786/150‑1 (MQ=255)
                                                                                                          cTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCt                                        >  2:2459473/1‑150 (MQ=255)
                                                                                                             cATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAAt                                     <  1:1393764/150‑1 (MQ=255)
                                                                                                              aTTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATg                                    >  2:3406636/1‑150 (MQ=255)
                                                                                                                tGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggc                                  >  2:1147245/1‑150 (MQ=255)
                                                                                                                    aCCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGgcggcg                              <  2:266986/150‑1 (MQ=255)
                                                                                                                        ccAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCACAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaaa                          <  2:1049864/150‑1 (MQ=255)
                                                                                                                           gACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCa                       <  2:1805089/150‑1 (MQ=255)
                                                                                                                                aaGATCGCCAGCGTTTTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACTTGCGCGCCAAAAGCGCCCAGAGCCACAATAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa                  <  1:526249/150‑1 (MQ=255)
                                                                                                                                      gCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACgg            >  1:1642773/1‑150 (MQ=255)
                                                                                                                                      gCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACgg            >  2:1594487/1‑150 (MQ=255)
                                                                                                                                            gTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTc      >  1:3993438/1‑150 (MQ=255)
                                                                                                                                             tATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCa     <  2:923306/150‑1 (MQ=255)
                                                                                                                                                gAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATgg  <  2:945301/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGG  >  CP009273/2934699‑2934992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑