Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,967,724 G→T 5.6% I550I (ATC→ATA)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,967,7240GT 5.6% 1015.2 / 23.5 268I550I (ATC→ATA) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base G (132/121);  new base T (4/11);  total (136/132)
Fisher's exact test for biased strand distribution p-value = 6.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 270 total aligned reads displayed.

CGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATC  >  CP009273/2967575‑2967873
                                                                                                                                                     |                                                                                                                                                     
cGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCg                                                                                                                                                       >  2:870757/1‑150 (MQ=255)
   acaATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATa                                                                                                                                                    <  2:188201/150‑1 (MQ=255)
     aaTATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCTATAcc                                                                                                                                                  <  1:378271/150‑1 (MQ=255)
     aaTATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATAcc                                                                                                                                                  <  2:2141075/150‑1 (MQ=255)
      atatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCa                                                                                                                                                 >  1:10288/1‑150 (MQ=255)
      atatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCa                                                                                                                                                 >  2:363044/1‑150 (MQ=255)
       tatCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAg                                                                                                                                                <  2:1831278/150‑1 (MQ=255)
         tCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAggg                                                                                                                                              <  2:516689/150‑1 (MQ=255)
          cGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGa                                                                                                                                             <  1:677302/150‑1 (MQ=255)
            ccAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGAcc                                                                                                                                           <  2:3060390/150‑1 (MQ=255)
            ccAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGAcc                                                                                                                                           <  2:1988113/150‑1 (MQ=255)
             cAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCg                                                                                                                                          <  2:3185408/150‑1 (MQ=255)
                tgtCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCTATACCAGGGACCGaca                                                                                                                                       <  1:1490735/150‑1 (MQ=255)
                  tCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGacaac                                                                                                                                     <  1:1630804/150‑1 (MQ=255)
                   cATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGacaaca                                                                                                                                    <  1:1738813/150‑1 (MQ=255)
                      aCCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATGCCAGGGACCGACAACAGgc                                                                                                                                 >  2:1842258/1‑150 (MQ=255)
                      aCCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGgc                                                                                                                                 >  1:2236745/1‑150 (MQ=255)
                       ccAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCTATACCAGGGACCGACAACAGgcg                                                                                                                                <  1:2095248/150‑1 (MQ=255)
                        cAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGgcgc                                                                                                                               <  2:245125/150‑1 (MQ=255)
                          gCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGgcgcgc                                                                                                                             >  1:821197/1‑150 (MQ=255)
                           ccGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCa                                                                                                                            >  1:4111348/1‑150 (MQ=255)
                            cgcgCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCAt                                                                                                                           >  1:1591693/1‑150 (MQ=255)
                             gcgcTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATc                                                                                                                          >  2:2308957/1‑150 (MQ=255)
                              cgcTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATcc                                                                                                                         >  1:3994287/1‑150 (MQ=255)
                                cTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCAt                                                                                                                       >  2:745506/1‑150 (MQ=255)
                                 tCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCAtt                                                                                                                      >  1:3285568/1‑150 (MQ=255)
                                  ccATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTc                                                                                                                     >  2:2055588/1‑150 (MQ=255)
                                   cATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTcc                                                                                                                    <  2:3461203/150‑1 (MQ=255)
                                    aTTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCt                                                                                                                   >  1:3606075/1‑150 (MQ=255)
                                    aTTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCt                                                                                                                   >  1:2646465/1‑150 (MQ=255)
                                    aTTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCt                                                                                                                   <  2:3540811/150‑1 (MQ=255)
                                      ttCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTgg                                                                                                                 <  1:4088822/150‑1 (MQ=255)
                                        cgccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTa                                                                                                               >  2:3696187/1‑150 (MQ=255)
                                         gccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAg                                                                                                              >  2:2527720/1‑150 (MQ=255)
                                          ccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCTATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGa                                                                                                             >  1:1860153/1‑150 (MQ=255)
                                                aCATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGc                                                                                                       <  1:1263992/150‑1 (MQ=255)
                                                aCATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGc                                                                                                       <  1:1264213/150‑1 (MQ=255)
                                                 cATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGcc                                                                                                      >  2:1107297/1‑150 (MQ=255)
                                                   ttCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    <  1:1303145/150‑1 (MQ=255)
                                                   ttCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    >  2:2674808/1‑150 (MQ=255)
                                                   ttCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    >  2:3231693/1‑150 (MQ=255)
                                                   ttCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTACGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    <  2:3822258/150‑1 (MQ=255)
                                                   ttCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTATTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    <  1:683859/150‑1 (MQ=255)
                                                   ttCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTGTCAGTTGCAGGCGTCCGCCCTCTTCTATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    >  2:1529334/1‑150 (MQ=255)
                                                   ttCTCGGCGGTGGGCACGTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTACGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCt                                                                                                    <  2:4448083/150‑1 (MQ=255)
                                                    tctcGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCta                                                                                                   <  2:617164/150‑1 (MQ=255)
                                                    tctcGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCAGTCCTGGTAGAATACGCCCta                                                                                                   <  2:2600970/150‑1 (MQ=255)
                                                     ctcGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCtac                                                                                                  <  2:3495780/150‑1 (MQ=255)
                                                      tcGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCtacc                                                                                                 <  2:3134726/150‑1 (MQ=255)
                                                             tGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCgg                                                                                          <  1:629086/150‑1 (MQ=255)
                                                              gggCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCggg                                                                                         >  2:1358795/1‑150 (MQ=255)
                                                                gCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGtt                                                                                       <  1:3748282/150‑1 (MQ=255)
                                                                  aCTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTc                                                                                     <  2:1465178/150‑1 (MQ=255)
                                                                   cTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCTATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTcg                                                                                    <  2:983498/150‑1 (MQ=255)
                                                                   cTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTcg                                                                                    >  1:2244614/1‑150 (MQ=255)
                                                                    ttCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTcgc                                                                                   >  1:1110273/1‑150 (MQ=255)
                                                                      ccAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTcgccg                                                                                 <  2:280150/150‑1 (MQ=255)
                                                                      ccAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTcgccg                                                                                 <  1:2883910/150‑1 (MQ=255)
                                                                         gTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCa                                                                              <  2:2340371/150‑1 (MQ=255)
                                                                           acacCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATc                                                                            <  2:2013685/150‑1 (MQ=255)
                                                                            cacCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCg                                                                           >  2:4386183/1‑150 (MQ=255)
                                                                            cacCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCg                                                                           >  1:4417667/1‑150 (MQ=255)
                                                                               cTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTa                                                                        <  1:2527720/150‑1 (MQ=255)
                                                                                         cACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGa                                                              <  1:1333911/150‑1 (MQ=255)
                                                                                           cccGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGaaa                                                            >  2:2846990/1‑150 (MQ=255)
                                                                                           cccGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGGTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGaaa                                                            <  2:3050516/150‑1 (MQ=255)
                                                                                             cGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACg                                                          >  1:3046394/1‑150 (MQ=255)
                                                                                               cAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGAc                                                        >  2:50707/1‑150 (MQ=255)
                                                                                               cAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGAc                                                        >  2:2705530/1‑150 (MQ=255)
                                                                                                 gATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAg                                                      >  1:732031/1‑150 (MQ=255)
                                                                                                   tAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCTATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGgc                                                    >  1:3319718/1‑150 (MQ=255)
                                                                                                   tAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGgc                                                    >  2:1517137/1‑150 (MQ=255)
                                                                                                   tAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGgc                                                    >  1:1039207/1‑150 (MQ=255)
                                                                                                   tAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGgc                                                    >  2:1030121/1‑150 (MQ=255)
                                                                                                    aGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGgcg                                                   >  1:3587634/1‑150 (MQ=255)
                                                                                                        gTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCa                                               <  2:3499845/150‑1 (MQ=255)
                                                                                                        gTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCa                                               >  1:4268996/1‑150 (MQ=255)
                                                                                                          tCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCAtt                                             >  1:529789/1‑150 (MQ=255)
                                                                                                           ccttATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTc                                            <  2:3999755/148‑1 (MQ=255)
                                                                                                           cATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTc                                            <  2:4214476/150‑1 (MQ=255)
                                                                                                             ttATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCgg                                          >  1:3500369/1‑150 (MQ=255)
                                                                                                             ttATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCgg                                          >  2:3194010/1‑150 (MQ=255)
                                                                                                               aTGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTc                                        >  1:3209951/1‑150 (MQ=255)
                                                                                                               aTGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTc                                        <  2:3428448/150‑1 (MQ=255)
                                                                                                                tGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCa                                       <  2:486818/150‑1 (MQ=255)
                                                                                                                tGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTACGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCa                                       <  1:1659007/150‑1 (MQ=255)
                                                                                                                 gTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCAc                                      >  1:4488808/1‑150 (MQ=255)
                                                                                                                   tCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGc                                    >  1:2606726/1‑150 (MQ=255)
                                                                                                                    cGGCCCTTTCAGTTGCAGGCGTCCGCCCTCATCGAAACCAGGGACCGACAACAGGCGCGCATCCATACCTGGTAGAATAAGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATACGGTCACGcc                                   >  1:1684552/1‑150 (MQ=255)
                                                                                                                       cccTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTa                                <  1:3868789/150‑1 (MQ=255)
                                                                                                                       cccTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTa                                <  1:2902846/150‑1 (MQ=255)
                                                                                                                            tCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCtt                           >  2:46641/1‑150 (MQ=255)
                                                                                                                             cAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTc                          >  2:3010442/1‑150 (MQ=255)
                                                                                                                                 tGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAgg                      >  1:2716078/1‑150 (MQ=255)
                                                                                                                                    aGGCGTCCGCCCTCTTCGAGACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACTACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATg                   <  2:1754492/150‑1 (MQ=255)
                                                                                                                                     ggCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATgc                  <  1:2048744/150‑1 (MQ=255)
                                                                                                                                      gCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATgcg                 <  1:1843480/150‑1 (MQ=255)
                                                                                                                                             cccTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCa          <  2:2855759/150‑1 (MQ=255)
                                                                                                                                                 ctTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAAtt      <  1:4497509/150‑1 (MQ=255)
                                                                                                                                                    cGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTAt   >  1:642075/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCACAATATCGCCAGTGTCATACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATC  >  CP009273/2967575‑2967873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑