BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,085,649	C→A	5.4%	T138K (ACG→AAG)	gshB →	glutathione synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,085,649	0	C	A	5.4%	1055.8 / 23.8	277	T138K (ACG→AAG)	gshB	glutathione synthetase
Reads supporting (aligned to +/- strand): ref base C (124/138); new base A (9/6); total (133/144)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 288 total aligned reads displayed.

CCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCCTGA  >  CP009273/3085500‑3085798

cccGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAc                                                                                                                                                       <  2:3959222/150‑1 (MQ=255)

 ccgccgTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACg                                                                                                                                                      >  2:1405495/1‑150 (MQ=255)

 ccgccgTTTGATACCGAGTTTATCTACGAGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACg                                                                                                                                                      <  2:1969891/150‑1 (MQ=255)

   gccgTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCt                                                                                                                                                    <  1:1041625/150‑1 (MQ=255)

     cgTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTgg                                                                                                                                                  >  1:4194726/1‑150 (MQ=255)

      gTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGt                                                                                                                                                 >  2:707705/1‑150 (MQ=255)

      gTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGt                                                                                                                                                 >  2:2928297/1‑150 (MQ=255)

       tttGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGtt                                                                                                                                                <  2:4293823/150‑1 (MQ=255)

        ttGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTa                                                                                                                                               <  1:2355342/150‑1 (MQ=255)

         tGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAc                                                                                                                                              <  2:2831885/150‑1 (MQ=255)

         tGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAc                                                                                                                                              >  2:1759803/1‑150 (MQ=255)

          gATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAcg                                                                                                                                             <  2:374429/150‑1 (MQ=255)

              ccGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCa                                                                                                                                         <  1:3959567/150‑1 (MQ=255)

               cGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCaa                                                                                                                                        >  1:3799285/1‑150 (MQ=255)

               cGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCaa                                                                                                                                        >  1:2213420/1‑150 (MQ=255)

                gAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAAt                                                                                                                                       <  1:1758434/150‑1 (MQ=255)

                 aGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATa                                                                                                                                      >  2:1255312/1‑150 (MQ=255)

                    ttATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAg                                                                                                                                   >  1:637321/1‑150 (MQ=255)

                     tATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAgc                                                                                                                                  >  1:3444344/1‑150 (MQ=255)

                      aTCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAgcg                                                                                                                                 >  1:330971/1‑150 (MQ=255)

                      aTCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAgcg                                                                                                                                 <  1:1115255/150‑1 (MQ=255)

                         tACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAg                                                                                                                              >  2:3401684/1‑150 (MQ=255)

                         tACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAg                                                                                                                              >  2:961514/1‑150 (MQ=255)

                         tACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAg                                                                                                                              <  1:2639069/150‑1 (MQ=255)

                           cgcgACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCt                                                                                                                            <  1:1082056/150‑1 (MQ=255)

                            gcgACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTa                                                                                                                           <  2:3151532/150‑1 (MQ=255)

                              gACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTaaa                                                                                                                         >  2:4139954/1‑150 (MQ=255)

                              gACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTaaa                                                                                                                         >  2:2075809/1‑150 (MQ=255)

                               aCCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTaaaa                                                                                                                        <  1:436396/150‑1 (MQ=255)

                                 cTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGc                                                                                                                      <  1:1395211/150‑1 (MQ=255)

                                  tataTTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCg                                                                                                                     >  1:2731924/1‑150 (MQ=255)

                                   atatTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGt                                                                                                                    >  2:1349055/1‑150 (MQ=255)

                                    tatTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGtt                                                                                                                   >  1:1361988/1‑150 (MQ=255)

                                      ttCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCt                                                                                                                 <  2:2484047/150‑1 (MQ=255)

                                      ttCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCt                                                                                                                 <  2:790986/150‑1 (MQ=255)

                                        cTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTgg                                                                                                               <  2:3871584/150‑1 (MQ=255)

                                          ggAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGga                                                                                                             <  1:1865092/150‑1 (MQ=255)

                                          ggAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGga                                                                                                             >  2:1938588/1‑150 (MQ=255)

                                           gAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgag                                                                                                            <  1:3672709/150‑1 (MQ=255)

                                           gAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgag                                                                                                            <  2:51535/150‑1 (MQ=255)

                                           gAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgag                                                                                                            <  1:71624/150‑1 (MQ=255)

                                              cGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGaaa                                                                                                         <  2:2595844/150‑1 (MQ=255)

                                                tGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAaca                                                                                                       <  2:3264317/150‑1 (MQ=255)

                                                 gCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAacac                                                                                                      >  1:3556289/1‑150 (MQ=255)

                                                  ccGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAAcaca                                                                                                     <  1:1987214/150‑1 (MQ=255)

                                                    gAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGc                                                                                                   <  2:3446902/150‑1 (MQ=255)

                                                       gagaAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAc                                                                                                <  1:2976085/150‑1 (MQ=255)

                                                       gagaAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAc                                                                                                >  1:3214097/1‑150 (MQ=255)

                                                         gaAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcat                                                                                              <  1:795250/150‑1 (MQ=255)

                                                             gggACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCAtt                                                                                          <  2:3161239/150‑1 (MQ=255)

                                                                  gCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTaa                                                                                     <  1:1691501/150‑1 (MQ=255)

                                                                     gATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGcc                                                                                  <  2:835984/150‑1 (MQ=255)

                                                                        cGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCt                                                                               <  2:3135767/150‑1 (MQ=255)

                                                                        cGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCt                                                                               >  2:1437673/1‑150 (MQ=255)

                                                                         gTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTg                                                                              <  1:3114653/150‑1 (MQ=255)

                                                                         gTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTg                                                                              <  1:684489/150‑1 (MQ=255)

                                                                           tAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGa                                                                            <  2:2651772/150‑1 (MQ=255)

                                                                                   cGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATgg                                                                    <  2:2079573/150‑1 (MQ=255)

                                                                                      agagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTCTGggcg                                                                 >  2:3559532/1‑150 (MQ=255)

                                                                                      agagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcg                                                                 >  2:3458779/1‑150 (MQ=255)

                                                                                       gagCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcgg                                                                >  1:1942057/1‑150 (MQ=255)

                                                                                           cTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGcgcg                                                            >  2:1694449/1‑150 (MQ=255)

                                                                                            tGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGt                                                           >  1:2237508/1‑150 (MQ=255)

                                                                                                  aCTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAttt                                                     >  1:2116535/1‑150 (MQ=255)

                                                                                                   cTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtttt                                                    >  2:2733263/1‑150 (MQ=255)

                                                                                                     gTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTcc                                                  >  2:4529044/1‑150 (MQ=255)

                                                                                                     gTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTcc                                                  <  1:4102852/150‑1 (MQ=255)

                                                                                                     gTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTAGGGGCGGCGCGTCGATTTTcc                                                  >  2:1512083/1‑150 (MQ=255)

                                                                                                         cGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGt                                              >  1:443197/1‑150 (MQ=255)

                                                                                                         cGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGt                                              >  2:3691430/1‑150 (MQ=255)

                                                                                                           agaAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGa                                            >  1:1709818/1‑150 (MQ=255)

                                                                                                             aaaCTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGaaa                                          <  1:2862241/150‑1 (MQ=255)

                                                                                                               aCTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaaga                                        >  2:3190565/1‑150 (MQ=255)

                                                                                                               aCTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaaga                                        >  1:2817103/1‑150 (MQ=255)

                                                                                                               aCTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaaga                                        <  2:1147384/150‑1 (MQ=255)

                                                                                                                cTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaagaa                                       >  2:4001708/1‑150 (MQ=255)

                                                                                                                  gatgaCCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAgg                                     <  1:2415636/146‑1 (MQ=255)

                                                                                                                  gTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAgg                                     >  1:3632560/1‑150 (MQ=255)

                                                                                                                  gTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAgg                                     >  2:1120796/1‑150 (MQ=255)

                                                                                                                  gTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAgg                                     <  1:4260602/150‑1 (MQ=255)

                                                                                                                       ccGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATc                                <  1:80719/150‑1 (MQ=255)

                                                                                                                       ccGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATc                                <  1:1437673/150‑1 (MQ=255)

                                                                                                                           cTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCaaa                            >  1:2897720/1‑150 (MQ=255)

                                                                                                                            tGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAc                           <  1:2575103/150‑1 (MQ=255)

                                                                                                                              gTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCt                         <  1:3477948/150‑1 (MQ=255)

                                                                                                                                tctcTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCg                       <  2:3514913/150‑1 (MQ=255)

                                                                                                                                tctcTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCg                       <  1:3394661/150‑1 (MQ=255)

                                                                                                                                tctcTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCg                       >  1:950073/1‑150 (MQ=255)

                                                                                                                                tctcTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCg                       <  1:3447824/150‑1 (MQ=255)

                                                                                                                                  tctGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGc                     >  2:253618/1‑150 (MQ=255)

                                                                                                                                    tGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGt                   <  2:3388352/150‑1 (MQ=255)

                                                                                                                                       cTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGAt                >  1:1148365/1‑150 (MQ=255)

                                                                                                                                       cTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGAt                >  2:2333320/1‑150 (MQ=255)

                                                                                                                                         tAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTg              >  2:1684890/1‑150 (MQ=255)

                                                                                                                                          aaCGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGc             >  2:3261196/1‑150 (MQ=255)

                                                                                                                                            cGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCg           <  1:1567780/150‑1 (MQ=255)

                                                                                                                                                   aaCGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCct    <  2:4443834/150‑1 (MQ=255)

                                                                                                                                                   aaCGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCct    >  2:2197501/1‑150 (MQ=255)

                                                                                                                                                     cGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCctga  <  2:1362039/150‑1 (MQ=255)

                                                                                                                                                     cGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATGCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCctga  >  2:3794290/1‑150 (MQ=255)

CCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCCTGA  >  CP009273/3085500‑3085798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`