Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,097,200 C→A 8.2% R277R (CGG→AGG)  mutY → adenine DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,097,2000CA 8.2% 993.2 / 41.2 269R277R (CGG→AGG) mutYadenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (122/125);  new base A (5/17);  total (127/142)
Fisher's exact test for biased strand distribution p-value = 2.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 276 total aligned reads displayed.

CGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAG  >  CP009273/3097051‑3097347
                                                                                                                                                     |                                                                                                                                                   
cGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                                                                                                                                                     <  1:2218733/150‑1 (MQ=255)
cGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                                                                                                                                                     >  1:64543/1‑150 (MQ=255)
cGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAc                                                                                                                                                     <  2:666643/150‑1 (MQ=255)
   tGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGc                                                                                                                                                  >  1:2256834/1‑150 (MQ=255)
    gCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCa                                                                                                                                                 <  2:2759846/150‑1 (MQ=255)
    gCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCa                                                                                                                                                 <  2:2527893/150‑1 (MQ=255)
       ggAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGAt                                                                                                                                              <  2:3797195/150‑1 (MQ=255)
       ggAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGAt                                                                                                                                              >  1:4416722/1‑150 (MQ=255)
        gAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGAGTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGAtt                                                                                                                                             >  2:1155649/1‑150 (MQ=255)
         aGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtg                                                                                                                                            >  1:2870578/1‑150 (MQ=255)
         aGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtg                                                                                                                                            <  1:2377293/150‑1 (MQ=255)
           cgcACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtgct                                                                                                                                          >  2:1509423/1‑150 (MQ=255)
           cgcACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtgct                                                                                                                                          <  2:736270/150‑1 (MQ=255)
            gcACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtgctg                                                                                                                                         >  2:3057851/1‑150 (MQ=255)
            gcACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtgctg                                                                                                                                         >  1:3032357/1‑150 (MQ=255)
             cACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATtgctgc                                                                                                                                        >  2:1395076/1‑150 (MQ=255)
                cGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGa                                                                                                                                     >  1:1536734/1‑150 (MQ=255)
                cGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGa                                                                                                                                     >  1:1538424/1‑150 (MQ=255)
                cGGCTACTTTGTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTGTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGa                                                                                                                                     <  2:2752966/150‑1 (MQ=255)
                  gCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATa                                                                                                                                   >  1:2652481/1‑150 (MQ=255)
                   cTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATaa                                                                                                                                  >  1:4396031/1‑150 (MQ=255)
                   cTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATaa                                                                                                                                  >  1:708435/1‑150 (MQ=255)
                    tACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAAc                                                                                                                                 <  2:4445803/150‑1 (MQ=255)
                    tACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAAc                                                                                                                                 >  1:4139681/1‑150 (MQ=255)
                      cTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCt                                                                                                                               >  1:3137955/1‑150 (MQ=255)
                      cTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCt                                                                                                                               >  2:100816/1‑150 (MQ=255)
                      cTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTACGCCGAGCGGATTGTGGGGCGGTTTAAACTGTTTCCCGCAGATTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCt                                                                                                                               >  1:2931261/1‑150 (MQ=255)
                          ttGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACg                                                                                                                           <  1:1171518/150‑1 (MQ=255)
                           tGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGc                                                                                                                          >  2:3959392/1‑150 (MQ=255)
                            gCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCa                                                                                                                         <  2:3053322/150‑1 (MQ=255)
                            gCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCa                                                                                                                         >  1:518853/1‑150 (MQ=255)
                            gCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCa                                                                                                                         <  2:3055118/150‑1 (MQ=255)
                               aTTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACt                                                                                                                      <  1:3437318/150‑1 (MQ=255)
                               aTTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACt                                                                                                                      <  2:1445563/150‑1 (MQ=255)
                                          aaGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTc                                                                                                           >  2:3257286/1‑150 (MQ=255)
                                           aGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAAGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCg                                                                                                          <  1:1944602/150‑1 (MQ=255)
                                               gAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCAt                                                                                                      <  1:1474019/150‑1 (MQ=255)
                                                 aGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATAc                                                                                                    <  2:2269495/150‑1 (MQ=255)
                                                 aGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATAc                                                                                                    <  1:1349466/150‑1 (MQ=255)
                                                  gTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATAcc                                                                                                   >  2:2112870/1‑150 (MQ=255)
                                                    aTTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCtt                                                                                                 >  1:2734064/1‑150 (MQ=255)
                                                    aTTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCtt                                                                                                 <  1:773647/150‑1 (MQ=255)
                                                    aTTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCtt                                                                                                 <  1:773681/150‑1 (MQ=255)
                                                     ttGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTc                                                                                                <  2:363520/150‑1 (MQ=255)
                                                     gtGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTc                                                                                                <  2:364106/149‑1 (MQ=255)
                                                      tGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCa                                                                                               <  2:662075/150‑1 (MQ=255)
                                                       gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAg                                                                                              <  2:1956000/150‑1 (MQ=255)
                                                       gCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAg                                                                                              <  2:1956227/150‑1 (MQ=255)
                                                               aGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTcc                                                                                      >  2:480263/1‑150 (MQ=255)
                                                               aGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAAGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCAttttc                                                                                      >  2:923257/1‑148 (MQ=255)
                                                                 cGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCAc                                                                                    >  2:3081991/1‑150 (MQ=255)
                                                                 cGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCAc                                                                                    <  1:365003/150‑1 (MQ=255)
                                                                    ccgccgAGCGGTTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGATAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACTCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCTCTTa                                                                                 <  2:3219829/150‑1 (MQ=255)
                                                                    ccgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTa                                                                                 >  1:1696545/1‑150 (MQ=255)
                                                                     cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAg                                                                                <  2:4087030/150‑1 (MQ=255)
                                                                     cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAg                                                                                <  2:2655243/150‑1 (MQ=255)
                                                                      gccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGa                                                                               >  1:189317/1‑150 (MQ=255)
                                                                       ccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGat                                                                              <  1:1940067/150‑1 (MQ=255)
                                                                       ccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTGTCGGCATACCTTCAGCCATTTTCACTTAGat                                                                              <  2:2119602/150‑1 (MQ=255)
                                                                        cgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAAGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGata                                                                             <  1:970927/150‑1 (MQ=255)
                                                                          aGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATAtt                                                                           <  1:2883597/150‑1 (MQ=255)
                                                                            cGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATtgt                                                                         >  2:607459/1‑150 (MQ=255)
                                                                                 tgtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTa                                                                    <  2:4324998/150‑1 (MQ=255)
                                                                                  gtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAt                                                                   <  1:3755448/150‑1 (MQ=255)
                                                                                   tgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCGCTTAGATATTGTGCCTAtg                                                                  <  1:2746276/150‑1 (MQ=255)
                                                                                   tgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAAGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAtg                                                                  >  1:2577205/1‑150 (MQ=255)
                                                                                    ggggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAtgt                                                                 <  1:2091576/150‑1 (MQ=255)
                                                                                    ggggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAtgt                                                                 <  1:4337906/150‑1 (MQ=255)
                                                                                    ggggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTAtgt                                                                 <  1:3539157/150‑1 (MQ=255)
                                                                                       gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGc                                                              <  1:335916/150‑1 (MQ=255)
                                                                                         ggTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCtt                                                            <  1:3982041/150‑1 (MQ=255)
                                                                                          gTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTc                                                           <  2:2237264/150‑1 (MQ=255)
                                                                                             tataCTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCg                                                        >  1:849349/1‑150 (MQ=255)
                                                                                                      tCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAt                                               <  2:596736/150‑1 (MQ=255)
                                                                                                        ccGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTc                                             <  1:793072/150‑1 (MQ=255)
                                                                                                        ccGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAAAGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTc                                             <  2:1698166/150‑1 (MQ=255)
                                                                                                         cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCa                                            <  2:809352/150‑1 (MQ=255)
                                                                                                           cAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCAcc                                          <  2:1751804/150‑1 (MQ=255)
                                                                                                           cAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCAcc                                          <  1:3616545/150‑1 (MQ=255)
                                                                                                             gTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                        >  1:2398392/1‑150 (MQ=255)
                                                                                                                tGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTg                                     >  2:4050409/1‑150 (MQ=255)
                                                                                                                    gacgaAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATg                                 <  2:1890186/150‑1 (MQ=255)
                                                                                                                     acgaAGAAAGTTTGCGGCAGTGGCTGGCGCAAAGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATgg                                <  1:913368/150‑1 (MQ=255)
                                                                                                                       gaagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAt                              >  1:3380292/1‑150 (MQ=255)
                                                                                                                         agaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGa                            <  2:3106883/150‑1 (MQ=255)
                                                                                                                          gaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGaa                           <  1:4480042/150‑1 (MQ=255)
                                                                                                                           aaaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAg                          <  2:3334669/150‑1 (MQ=255)
                                                                                                                            aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAgg                         <  1:2502907/150‑1 (MQ=255)
                                                                                                                            aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAgg                         >  1:57884/1‑150 (MQ=255)
                                                                                                                            aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAgg                         >  1:57955/1‑150 (MQ=255)
                                                                                                                             aGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGGGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGc                        <  1:4476305/150‑1 (MQ=255)
                                                                                                                             aGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGc                        <  2:1739259/150‑1 (MQ=255)
                                                                                                                                   cGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATgcg                  >  2:1205052/1‑150 (MQ=255)
                                                                                                                                   cGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAGTCACCGGCTGCATGGATGAAGGCAATgcg                  >  2:2947773/1‑150 (MQ=255)
                                                                                                                                    ggCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATgcgc                 >  2:2430260/1‑150 (MQ=255)
                                                                                                                                      cAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGctc               <  1:4517755/150‑1 (MQ=255)
                                                                                                                                         tggctggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTgg            >  2:3681947/1‑150 (MQ=255)
                                                                                                                                          ggctggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGt           <  2:4540352/150‑1 (MQ=255)
                                                                                                                                             tggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGtata        >  1:1062277/1‑150 (MQ=255)
                                                                                                                                                   aaCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAg  >  2:2692093/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAG  >  CP009273/3097051‑3097347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑