Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,237,654 C→A 6.6% M149I (ATG→ATT)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,237,6540CA 6.6% 1013.9 / 30.9 272M149I (ATG→ATT) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (125/128);  new base A (9/9);  total (135/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 283 total aligned reads displayed.

TCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAA  >  CP009273/3237505‑3237803
                                                                                                                                                     |                                                                                                                                                     
tCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTc                                                                                                                                                       >  2:3984455/1‑150 (MQ=255)
   ttAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATc                                                                                                                                                    <  2:71053/150‑1 (MQ=255)
     aaaGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctg                                                                                                                                                  >  2:268508/1‑150 (MQ=255)
       aGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgct                                                                                                                                                <  1:1165684/150‑1 (MQ=255)
       aGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgct                                                                                                                                                <  1:3437574/150‑1 (MQ=255)
        ggTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAACGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctgctg                                                                                                                                               <  2:3325664/150‑1 (MQ=255)
          tCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCa                                                                                                                                             >  2:4115021/1‑150 (MQ=255)
          tCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCa                                                                                                                                             <  1:2087983/150‑1 (MQ=255)
                gTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGc                                                                                                                                       <  1:3901641/150‑1 (MQ=255)
                  tCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcg                                                                                                                                     >  1:348935/1‑150 (MQ=255)
                    gATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcg                                                                                                                                   >  2:4199732/1‑150 (MQ=255)
                        ttGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcgcgcg                                                                                                                               >  2:666756/1‑150 (MQ=255)
                        ttGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcgcgcg                                                                                                                               >  2:510454/1‑150 (MQ=255)
                        ttGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcgcgcg                                                                                                                               >  2:4297899/1‑150 (MQ=255)
                           aaGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGgag                                                                                                                            >  2:3729895/1‑150 (MQ=255)
                              gCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAg                                                                                                                         <  1:2843735/150‑1 (MQ=255)
                               cTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGt                                                                                                                        >  1:379329/1‑150 (MQ=255)
                                tttGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGtt                                                                                                                       >  1:3931130/1‑150 (MQ=255)
                                     ccGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCt                                                                                                                  <  2:2816363/150‑1 (MQ=255)
                                      cGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTg                                                                                                                 >  2:1841765/1‑150 (MQ=255)
                                      cGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTg                                                                                                                 <  2:3862479/150‑1 (MQ=255)
                                      cGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTg                                                                                                                 >  2:3910667/1‑150 (MQ=255)
                                       ggACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgc                                                                                                                >  1:584462/1‑150 (MQ=255)
                                          cGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGcc                                                                                                             >  2:2867490/1‑150 (MQ=255)
                                           gCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCca                                                                                                            <  1:1440586/150‑1 (MQ=255)
                                           gCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCca                                                                                                            >  2:3400748/1‑150 (MQ=255)
                                                  cGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTc                                                                                                     <  1:2603174/150‑1 (MQ=255)
                                                   ggCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCg                                                                                                    >  2:841192/1‑150 (MQ=255)
                                                   ggCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCg                                                                                                    <  1:4528628/150‑1 (MQ=255)
                                                   ggCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCg                                                                                                    >  2:3482684/1‑150 (MQ=255)
                                                     cagcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTACAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGtt                                                                                                  <  2:2974260/150‑1 (MQ=255)
                                                       gcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTAc                                                                                                <  1:3771380/150‑1 (MQ=255)
                                                       gcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTAc                                                                                                <  1:383482/150‑1 (MQ=255)
                                                        caCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACa                                                                                               >  2:2528266/1‑150 (MQ=255)
                                                          cTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACAtt                                                                                             <  2:1204245/150‑1 (MQ=255)
                                                           tttGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTc                                                                                            <  2:1074233/150‑1 (MQ=255)
                                                            ttGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCg                                                                                           <  1:2727326/150‑1 (MQ=255)
                                                              gATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGtt                                                                                         <  2:1006668/150‑1 (MQ=255)
                                                                 ggTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCa                                                                                      >  2:3217391/1‑150 (MQ=255)
                                                                 ggTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCa                                                                                      >  1:555651/1‑150 (MQ=255)
                                                                      aaGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAttt                                                                                 >  1:3623061/1‑150 (MQ=255)
                                                                            cGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCgg                                                                           <  1:2536917/150‑1 (MQ=255)
                                                                             gTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGc                                                                          >  1:1041972/1‑150 (MQ=255)
                                                                             gTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGc                                                                          >  1:1768111/1‑150 (MQ=255)
                                                                                tttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGt                                                                       >  1:1635718/1‑150 (MQ=255)
                                                                                 ttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGtt                                                                      <  1:2931151/150‑1 (MQ=255)
                                                                                      gATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACgg                                                                 >  1:4350501/1‑150 (MQ=255)
                                                                                      gATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACgg                                                                 <  2:4252840/150‑1 (MQ=255)
                                                                                       aTCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGga                                                                <  1:1672372/150‑1 (MQ=255)
                                                                                       aTCTCTGCGTGATGCTACAGAGAATCGATAGGGTCATCGGTGGTGCAGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCTTTCCAGATTTCATCGGCAGTTGACGga                                                                >  2:3256074/1‑150 (MQ=255)
                                                                                        tctcTGCGTGATGCTCCAGAGACTCGATCGGGTCTTCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCCGATGTCATCGGCAGTGGACGgag                                                               <  2:3523254/150‑1 (MQ=255)
                                                                                           ctGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGaaa                                                            <  1:4290582/150‑1 (MQ=255)
                                                                                              cGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCa                                                         <  1:2241349/150‑1 (MQ=255)
                                                                                               gTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCaa                                                        >  1:2006252/1‑150 (MQ=255)
                                                                                               gTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCaa                                                        >  1:2006867/1‑150 (MQ=255)
                                                                                                  aTGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACTTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAttt                                                     <  2:230967/150‑1 (MQ=255)
                                                                                                   tGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTg                                                    >  2:3955146/1‑150 (MQ=255)
                                                                                                     cTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGTCGGAGAAAGCAATTTGcc                                                  >  2:95095/1‑150 (MQ=255)
                                                                                                     cTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGcc                                                  <  2:3302090/150‑1 (MQ=255)
                                                                                                     cTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGcc                                                  >  2:94479/1‑150 (MQ=255)
                                                                                                           agaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGAt                                            <  1:1836204/150‑1 (MQ=255)
                                                                                                            gaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATa                                           <  2:3782486/150‑1 (MQ=255)
                                                                                                            gaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATa                                           <  1:316250/150‑1 (MQ=255)
                                                                                                            gaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATa                                           <  1:3196138/150‑1 (MQ=255)
                                                                                                             aaTCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAc                                          >  1:3032659/1‑150 (MQ=255)
                                                                                                             aaTCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAc                                          >  1:3226195/1‑150 (MQ=255)
                                                                                                              atcgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAcc                                         >  2:1461239/1‑150 (MQ=255)
                                                                                                              atcgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAcc                                         >  2:328959/1‑150 (MQ=255)
                                                                                                                cgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaa                                       >  2:903263/1‑150 (MQ=255)
                                                                                                                 gatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaaa                                      <  1:3084649/150‑1 (MQ=255)
                                                                                                                  atcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAac                                     <  2:1124678/150‑1 (MQ=255)
                                                                                                                  atcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAac                                     <  2:1128513/150‑1 (MQ=255)
                                                                                                                  atcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAac                                     <  1:3766063/150‑1 (MQ=255)
                                                                                                                   tcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacg                                    <  2:4515401/150‑1 (MQ=255)
                                                                                                                     gggTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacgga                                  >  1:2434571/1‑150 (MQ=255)
                                                                                                                      ggTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggac                                 <  1:4236940/150‑1 (MQ=255)
                                                                                                                      ggTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggac                                 >  2:4376643/1‑150 (MQ=255)
                                                                                                                      ggTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggac                                 <  2:787230/150‑1 (MQ=255)
                                                                                                                      ggTCATCGGTGGTGCCGACCAATTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggac                                 >  2:1899372/1‑150 (MQ=255)
                                                                                                                       gTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacg                                <  2:597703/150‑1 (MQ=255)
                                                                                                                             ggtggtGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAg                          >  1:3240647/1‑150 (MQ=255)
                                                                                                                               tggtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGtt                        <  2:2911459/150‑1 (MQ=255)
                                                                                                                                  tgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGa                     >  1:3915776/1‑150 (MQ=255)
                                                                                                                                  tgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGa                     <  1:1879035/150‑1 (MQ=255)
                                                                                                                                    ccGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAgg                   >  2:1611970/1‑150 (MQ=255)
                                                                                                                                     cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGt                  <  1:1076263/150‑1 (MQ=255)
                                                                                                                                     cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGt                  >  2:3727593/1‑150 (MQ=255)
                                                                                                                                     cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGt                  <  1:708114/150‑1 (MQ=255)
                                                                                                                                      gACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTg                 >  1:60138/1‑150 (MQ=255)
                                                                                                                                        ccATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCg               >  2:3794704/1‑150 (MQ=255)
                                                                                                                                         cATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGATGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGt              >  1:993651/1‑150 (MQ=255)
                                                                                                                                           ttttCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTcc            >  1:2235039/1‑150 (MQ=255)
                                                                                                                                            tttCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCa           <  1:924699/150‑1 (MQ=255)
                                                                                                                                            tttCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCa           <  2:208763/150‑1 (MQ=255)
                                                                                                                                               cACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTg        >  1:1248049/1‑150 (MQ=255)
                                                                                                                                                 cGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGt      <  1:2368003/150‑1 (MQ=255)
                                                                                                                                                 cGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGt      >  2:899401/1‑150 (MQ=255)
                                                                                                                                                     cATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATaa  <  1:3819897/150‑1 (MQ=255)
                                                                                                                                                     cATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATaa  <  1:4115021/150‑1 (MQ=255)
                                                                                                                                                     cATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATaa  >  2:1171821/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATAA  >  CP009273/3237505‑3237803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑